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Production of gamma-aminobutyrate (GABA) in recombinant Corynebacterium glutamicum strains
박시재,김희택,주정찬 한국공업화학회 2018 한국공업화학회 연구논문 초록집 Vol.2018 No.0
Gamma-aminobutyrate (GABA) is an important precursor for synthesis of biodegradable polyamide 4 (PA4). Biorefinery for production of PA4 can be established by fermentative production of GABA from renewable resources using recombinant microorganisms. In this presentation, we report development of C. glutamicum strains expressing mutant glutamate decarboxylase. To develop recombinant strain capable of co-consumption of glucose and xylose, xylose utilization genes from E. coli was also introduced in recombinant C. glutamicum KCTC 1852. Detailed results will be presented. ** This work was supported by the Mid-career Researcher Program from the Ministry of Science and ICT (MSIT) through the National Research Foundation (NRF) of Korea (NRF-2016R1A2B4008707), a basic research grant from the KRIBB, and the Lignin Biorefinery from MSIT through the NRF of Korea (NRF- 2017M1A2A2087634).
박시재,오영훈,이상엽 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.0
As global concerns on the depletion of fossil resources and environmental pollutions increase, production of fuels and chemicals from bio-based materials has been attracting more attentions. In this presentation, we will report the development of metabolically engineered microorganisms for the production of bio-based plastics and chemicals that can be used as monomers for biopolymers. Detailed results about the development of microbial process for the production of lactate-containing PHAs such as PLA and 2HB containing PHAs and a hybrid process composed of biological production of nylon monomers precursors and chemical synthesis of nylon monomers that can be used for the synthesis of bio-based nylons will be presented. **Fund sources: NRF-2012-C1AAA001-2012M1A2A2026556, NRF-2013R1A1A2058379, Industrial Strategic Technology Development Program (10047910)]
Establishment of a biosynthesis pathway for (R)-3-hydroxyalkanoates in recombinant Escherichia coli
박시재,Seung Hwan Lee,오영훈,이상엽 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.4
A biosynthetic pathway for the production of (R)-3-hydroxyalkanoates (R3HAs) through in vivo depolymerizationof poly(3-hydroxyalkanoates) [P(3HAs)] was constructed in recombinant Escherichia coli fadA mutant WA101by introducing the Pseudomonas sp. 61-3 PHA synthase gene (phaC2) and the P. aeruginosa intracellular PHA depolymerasegene (phaZ). When recombinant E. coli WA101 strain expressing the phaC2 gene and the phaZ gene was culturedin Luria-Bertani (LB) medium containing 2 g/L of sodium decanoate, R3HAs could be produced to the concentrationof 0.49 g/L. The mole fraction of R3HAs was 7.5mol% of 3-hydroxybutyrate (3HB), 31.6mol% of 3-hydroxyhexanoate(3HHx), 30 mol% of 3-hydroxyoctanoate (3HO), 29.4mol% of 3-hydroxydecanoate (3HD), and 1.5mol% of3-hydroxydodecanoate (3HDD). When the E. coli 3-ketoacyl-ACP reductase gene (fabG) was overexpressed to providemore (R)-3-hydroxyacyl-CoA (R3HA-CoA), the concentration of R3HAs was increased up to 1.05 g/L. Also, expressionof the fabG gene resulted in the mole fraction change of produced R3HAs, in which 3HD fraction was enrichedfrom 29.4mol% to 57.9mol% with the decrease of 3HHx fraction from 31.6mol% to 9.6mol%. Interestingly, the onlyexpression of the fabG gene in E. coli WA101 could produce R3HAs to 0.55 g/L, which suggests that E. coli might haveunidentified CoA hydrolases that have substrate specificities toward R3HA-CoA. This study shows the enantiomericallypure RHAs can be efficiently produced by metabolically engineered E. coli with high yield.
2LJ-2 Development of recombinant Corynebacterium strains for the production of bio-nylons
박시재,케이앤바리투고,요키미코데이비드,정솔희,강경희,강태욱,현승민,김희택,이상엽,주정찬 한국공업화학회 2017 한국공업화학회 연구논문 초록집 Vol.2017 No.1
Biorefinery processes based on fermentative production employing microbial host strains have extensively been developed to substitute chemical processes based on fossil oils since target products produced from biomass-based renewable resources are carbon neutral products that can provide promising solutions for global warming. Microorganisms as host strains for fermentative production of target products have extensively been engineered by metabolic engineering strategies. Here, we will present the development of metabolically engineered Corynebacterium glutamicum strains for the production of bio-based nylon monomers precursors that can be used for the synthesis of bio- based nylons. <sup>**</sup>This work was supported by the R& D Program of MOTIE/KEIT (10049674) and the Mid-career Researcher Program from the Ministry of Science and ICT (MSIT) through the National Research Foundation (NRF) of Korea (NRF-2016R1A2B4008707)