수면이 균형조절과 시각적 자극 반응시간에 미치는 영향

박수경,박정아,박강희,김주헌,홍용근,Park, Sookyoung,Park, Jung-A,Park, Kanghui,Kim, Joo-Heon,Hong, Yonggeun 대한수면의학회 2016 수면·정신생리 Vol.23 No.2

목 적 : 수면은 신체의 기능을 회복시키고 항상성을 유지시키는 인체의 필수적인 생리작용으로, 수면의 질 저하는 전반적인 건강문제를 야기 시키는 것으로 보고되고 있다. 따라서 본 연구에서는 수면과 균형조절능력의 관계에 대해 규명하기 위하여, 수면이 균형조절과 시각적 반응시간에 미치는 영향에 대해 알아보고자 하였다. 방 법 : 본 연구에 참여하기를 서면 동의한 건강한 20대 남녀 58명을 대상으로 PSQI를 이용하여 수면의 질을 평가한 다음, PSQI 점수에 따라 대상자를 A군(PSQI <5)과 B군($PSQI{\geq}5$)으로 분류하였다. 이후 대상자들의 체성분과 신체특성, 균형조절, 시각적 반응시간 및 오류횟수를 각각 측정하였다. 결 과 : 수면의 질이 양호한 A군에 비해 수면의 질이 낮은 B군에서 아침기상 시 졸린 정도가 유의하게 높은 것으로 나타났다. 수면이 체성분에 미치는 영향에 대해 알아보기 위해 두 실험군의 신체조성을 비교한 결과, 수면의 질이 낮은 여자 대상자들의 체지방량과 체지방률이 유의하게 낮은 것으로 확인되었다. 또한 수면상태에 따른 균형을 비교한 결과, 유의한 차이가 나타나지 않았지만, 시각적 반응시간에서는 유의한 증가가 확인되었다. 특히 수면의 질이 낮은 B군에서 반응시간은 선형적으로 유의하게 증가하였으며, 오차횟수 역시 증가하는 것으로 나타났다. 결 론 : 수면의 질 저하는 반응속도를 지연시키고, 반응의 정확도를 감소시키는 것으로 나타났는데, 이러한 선택적 주의력의 저하는 균형조절에 영향을 끼칠 수 있고, 이로 인해 예상하지 못한 순간에 낙상 등의 손상을 입을 위험이 증가할 수 있다. Objectives: To find evidence that sleep is necessary for normal brain function, thus indicating that declines in both sleep quality and quantity are related to worse performance of many daily tasks and deteriorated physical functions. The present study investigates the relationships of balance control and reaction time with sleep quality. Methods: 58 healthy (male 20, female 38) volunteers with informed consent participated in this study. The Self-reported Pittsburgh Sleep Quality Index (PSQI) was used to evaluate sleep quality and relevant factors, and the subjects were divided into groups A (PSQI < 5) and B ($PSQI{\geq}5$) based on this index. Static balance control and reaction time to visual stimuli were conducted to assess their relationship with sleep quality. Results: Group B exhibited excessive daytime sleepiness significantly more often compared to group A. Static balance control did not markedly change relative to sleep quality, but reaction time and error to visual stimuli were significantly increased in group B compared to group A. Conclusion: These findings indicate that a decline in sleep quality can result in delayed reactions, as well as decreased accuracy of these reactions. They also suggest that low sleep quality may be associated with changes in physical functions, including balance control through reduced selective attention.

Profound Reduction of Motor Functions, Differential Expression of iNOS and Caveolins in Rat Brain with Focal Cerebral Ischemia

Sookyoung Park(박수경),Jinyoung Won,Min-Hee Rho,Rae-Jun Park,Jin-Sang Kim,Yonggeun Hong 한국실험동물학회 2009 한국실험동물학회 학술발표대회 논문집 Vol.2009 No.2

인체대장암세포주 SW480에 대한 재배 와송의 항암효과 연구

박수경(Sookyoung Park),원진영(Jinyoung Won),박강희(Kanghui Park),홍용근(Yonggeun Hong) 한국생명과학회 2018 생명과학회지 Vol.28 No.7

와송(둥근바위솔, Orostachys japonicus, OJ)은 면역조절, 항노화, 항산화, 독성제거 등의 치료적 효과를 가진 약용식물로 알려져 있는데, 본 연구에서는 인체대장암세포에 대한 재배 와송의 항암효과를 규명하고자 하였다. 인체대장암세포주 SW480에 와송열수추출물을 72시간 동안 처리한 결과, 대장암세포의 생존율은 농도-의존적으로 유의하게 감소하였다. 또한, 와송은 SW480 세포의 증식과 이주를 억제하는 것으로 나타났는데, 대조군의 스크래치 gap은 24시간부터 유의하게 감소하는 반면, 와송열수추출물을 처리한 실험군 I과 II의 스크래치 gap은 48시간부터 감소하기 시작하였다. 특히 실험군 I의 스크래치 gap은 72시간까지 유의하게 유지되었다. 와송이 생체 내에서 종양의 형성에 어떠한 영향을 미치는지 알아보기 위하여 대장암세포 주사 전, 31일 동안 수컷 C57BL/6 마우스(4주령)에게 와송열수추출물을 경구로 자유 섭취하게 하였다. 이후 SW480 세포(1×107 cells/100 μl)를 피하로 주입한 후 7일, 14일 그리고 28일에서 종양의 형성을 관찰하였고, 각 실험동물을 희생하여 종양의 무게와 부피(㎣)를 측정하여 비교 분석하였다. 와송열수추출물을 섭취하는 동안 실험군 I, II의 체중은 대조군에 비해 지속적으로 유의하게 증가하였다. SW480 세포 주입 이후 모든 실험군의 체중은 감소하였으나, 세포 주입 후 14일부터 와송 섭취 실험군의 체중은 유의하게 증가하는 것으로 나타났다. 대장암세포 주입 후 7일과 14일에서 와송을 섭취한 실험군의 종양 무게와 부피는 대조군보다 높았으나, 28일에서는 대조군보다 낮게 나타났고, 특히 실험군 II에서 종양 무게와 부피는 유의하게 감소하는 것으로 확인되었다. 이상의 결과를 통해 와송이 인체 대장암세포의 성장, 증식 및 이주를 억제하며, 생체 내 종양형성(tumorigenesis)을 예방적으로 억제함을 알 수 있었다. 따라서, 재배와송은 천연 항암제 소재로서 활용될 가능성을 가지는 것으로 보이며, 이에 대한 심층적인 연구가 필요할 것으로 사료된다. Orostachys japonicus (OJ) is a medicinal herb with immunoregulatory, anti-aging, anti-oxidative, and many other therapeutic properties. The purpose of this study was to elucidate the anti-cancer property of cultivated OJ. SW480 cell viability was significantly reduced by cumulative exposure to OJ extract. We also observed inhibitory effects of OJ after 72 hr through the growth and migration of SW480 cells using scratch assay. SW480 cells in OJ-free medium began to move into the scratch site at 24 hr; however, cells in medium containing OJ did not migrate into the scratch site until 48 hr. Male C57BL/6 mice (4 weeks old) were orally administered OJ extract for 31 days before injection of SW480 cells. At 7, 14, and 28 days after subcutaneous injection of SW480 cells, tumor weight and volume were analyzed. The body weight of the OJ-treated group was continuously increased during administration of the OJ extract relative to the control group. Injection of SW480 cells caused a reduction in body weight in all groups; however, the OJ-treated group exhibited a significant increase in body weight after 14 days. Tumor weight and volume were lower in the OJ-treated group than in the control group after 28 days. Although these results suggest that OJ suppresses the proliferation and migration of human colon cancer cells, additional studies are required to provide preclinical evidence before launching clinical trials evaluating OJ as an anti-cancer biohealth product.

Neuroprotective Effects of Forced Exercise on Focal Cerebral Ischemic Brain Damage of Spontaneously Hypertensive Rat

Sookyoung Park(박수경),Seunghoon Lee,Yunkyung Hong,Yonggeun Hong 한국실험동물학회 2009 한국실험동물학회 학술발표대회 논문집 Vol.2009 No.2

젊은 남성에서 부하 적용이 어깨 벌림 동안 봉우리밑 공간 간격에 미치는 영향

박강희 ( Park Kanghui ),박한규 ( Park Hankyu ),박수경 ( Park Sookyoung ) 대한통합의학회 2020 대한통합의학회지 Vol.8 No.1

Purpose : This study was performed to comparison of distance change of subacromial space according to shoulder abduction angle in loaded and unloaded conditions. Methods : The subjects were 20 male college students, and the subacromial space was measured using an ultrasound diagnostic apparatus. The participants shoulder abduction was measured during each shoulder resting position and 45 ° abduction with loads of 1 ㎏, 2 ㎏, 3 ㎏. The collected data were analyzed by a paired t-test and repeated measure ANOVA with the SPSS (Ver. 22) program. Results : The subacromial space showed statistically significant decreased in 45 ° abduction than resting position (p<.001). All the subacromial space increased during the according to loads (1 ㎏, 2 ㎏, 3 ㎏) at the resting position but at 45 ° abduction showed statistically significant decreased as the load increases (p<.05). Also, subacromial space showed significant changes in the 2㎏, 3㎏ compared with the 0㎏ loads. Conclusion : These results identified that shoulder abduction angle and load were related to subacromial space. When resting position, subacromial space is larger at loads than unload. As the load and shoulder abduction angle increase, subacromial space is reduced compared to resting.

초등학생의 스마트폰 사용과 체성분, 자세 및 근 속성에 대한 관계 분석

박시은 ( Park Sieun ),박수경 ( Park Sookyoung ) 대한통합의학회 2020 대한통합의학회지 Vol.8 No.3

Purpose : The purpose of this study was to determine the correlation between smartphone use, body composition, posture and muscle properties of elementary school students. Methods : A total of 22 healthy elementary school students in Daejeon, South Korea, participated in this study with informed consent. Smartphone use was evaluated with Smartphone addiction self-report scale (S-scale) for youth. Body composition (BMI, percent body fat, body fat mass, and skeletal muscle mass) was measured using Inbody. To assess posture, forward head posture and round shoulder posture were measured using Dartfish motion analysis. Muscle properties (muscle tone, stiffness, and elasticity) were measured on the upper trapezius, splenius capitis and sternocleidomastoid muscles using Myoton. To investigate the correlation between the main variables, Pearson correlation analysis was performed. Results : Disturbance of adaptive function, which is one of sub-component of S-scale, had a weak positive correlations with BMI (r=.538, p<.01), and body fat mass (r=.453, p<.05). However, scores of S-scale showed no correlation with posture and muscle properties. The correlation between posture and muscle properties was analyzed, and round shoulder posture revealed a weak positive correlation with respect to stiffness (r=.418, p<.05) and elasticity (r=.502, p<.05) of the sternocleidomastoid muscle. Conclusion : Scores of S-scale demonstrated a weak correlation with body composition (BMI) among elementary school students, whereas S-scale scores were not correlated with the posture and muscle properties. Further research is needed to identify and focus on potential high-risk user groups of elementary students who are more dependent upon smartphones.

3-Acetylpyridine에 의한 운동실조 동물모델에서 로타로드 운동과 전침이 근활성도와 혈청 BDNF에 미치는 영향

노민희(Min-Hee Rho),박수경(Sookyoung Park) 한국콘텐츠학회 2010 한국콘텐츠학회논문지 Vol.10 No.4

3-Acetylpyridine(3-AP)는 아래올리브핵을 선택적으로 파괴함으로써, 소뇌 손상을 유발하는 신경독소이다. 본 연구에서는 3-AP의 투여로 운동실조가 유발된 동물모델에서 로타로드 운동과 전침이 후지의 근활성도와 혈청 BDNF에 미치는 영향을 알아보고자 하였다. 본 연구를 위해 실험동물을 대조군, 3-AP군, 운동군, 전침군 그리고 운동전침군으로 무작위 배치하였다. 3-AP군의 최대점핑수직높이는 대조군에 비해 유의하게 감소하였고, 로타로드 운동과 전침의 적용 이후 점차 증가하는 것으로 나타났다. 또한 후지의 근활성도는 3-AP의 투여로 유의하게 증가하였고, 치료적 중재 이후 약간의 감소를 보였다. 3-AP군의 혈청 BDNF 농도는 대조군보다 유의하게 증가하였고, 운동군, 전침군 그리고 운동전침군에서는 3-AP군 보다 감소하는 것으로 나타났다. 이러한 결과를 통해 로타로드 운동과 전침은 운동실조 동물모델의 기능적 회복에 긍정적인 치료효과를 가지는 것으로 생각된다. The 3-Acetylpyridine(3-AP) induces cerebellar injury which is chemoablation of the inferior olive nucleus. The purpose of this study was to investigate the effects of exercise(Ex) and electroacupuncture(EA) on muscle activity of hindlimb and serum brain derived neurotrophic factor(BDNF) in the ataxia rats by the 3-AP. 12-week-aged male Sprague-Dawley rats were randomly divided into the 5 groups: Control, 3-AP, 3-AP+Ex, 3-AP+EA and 3-AP+Ex+EA groups. Maximal Height Vertical Jump(MHVJ) was significantly decreased in the 3-AP compared with control group, and it was increased in Ex, EA and Ex+EA groups than 3-AP group. The muscle activity of hindlimb was significantly increased in the 3-AP groups than control group, also it was most decreased in Ex+EA group. The concentration of BDNF of the serum was significantly decreased in the Ex, EA and Ex+EA groups than 3-AP group. The results of this study showed that dynamic exercise and electroacupuncture have a positive effect on functional recovery and in the ataxia rats by the 3-AP.

Toll-like Receptor 4-mediated Apoptotic Cell Death in Primary Isolated Human Cervical Cancers

Jinyoung Won(원진영),Yunkyung Hong(홍윤경),Sookyoung Park(박수경),Joo-Heon Kim(김주헌),Yonggeun Hong(홍용근) 한국생명과학회 2018 생명과학회지 Vol.28 No.6

Toll 유사수용체의 TLR4는 세포자연사(apoptosis)와 관련하여 세포의 생존과 증식에 영향을 미치는 것으로 알려져 있다. 본 연구에서는 TLR4의 활성이 부인과 질환 특이적 종양세포의 세포사멸기작에 어떠한 영향을 미치는지 살펴보았다. TLR4의 활성에 의한 세포자연사를 확인하기 위하여 부인암 조직(자궁경부암, 자궁내막암, 난소암)에서 종양세포를 분리하여 초대배양시스템을 구축하였고, lipopolysaccharide (LPS)에 의한 TLR4의 활성유도 과정에서 종양세포의 형태학적 변화를 살펴보았다. 또한, TLR4 매개성 세포사멸 기작을 확인하기 위하여 역전사 중합효소 연쇄반응(RT-PCR)을 통해 유전자 분석을 진행하였다. 연구 결과, 부인암의 초대배양세포에서 세포접촉저지(contact inhibition)현상이 감소되었고, 세포의 배가시간(doubling time)이 단축되어, 종양세포의 성장률 변화를 확인하였다(p<0.05). 자궁근육층(정상조직)의 초대배양세포에서는 민무늬근육 확인 인자인 ITGA5 (an alpha5 integrin marker)의 유전자 발현이 나타났으나, 자궁경부조직의 초대배양세포에서는 발현변화를 확인할 수 없었다. 종양세포의 유전자분석 결과에서 p53과 같은 종양억제인자의 발현이 유의적으로 증가한 반면(p<0.05), 세포사멸 신호기작과 관련하여 TLR4와 Caspase-3의 발현은 감소하였다(Caspase-3, p<0.05). LPS를 처리한 종양세포에서는 LPS 비처리군과 비교 시, TLR4의 발현증가와 함께 Caspase-3의 발현변화가 동반되었다. 이러한 결과들은 TLR4 매개성 apoptosis 유도가 종양세포의 증식억제에 중요한 영향을 미치는 것을 의미하며, TLR4 신호기작을 이용한 종양세포의 새로운 치료적 접근법을 제시할 것으로 기대한다. Toll-like receptor 4 (TLR4) has been implicated in cell proliferation and apoptosis in several types of cancer. In this study, the impact of TLR4 activation on apoptotic cell death in gynecologic cancers induced by lipopolysaccharide (LPS) was investigated. Cervical cancer cell lines were produced from isolated surgical specimens supplied by Paik Hospital. The primary cultures of normal myometrium and gynecologic cancers, including cervical, endometrial, and ovarian cancers, were used to examine the differences in morphological characteristics between normal and cancerous cells. A reverse transcription polymerase chain reaction analysis was used to determine the relative expression levels of TLR4 gene involved in apoptosis-associated signaling in cervical cancer cells. The cancer cell colonies showed a tendency to reach high levels of confluency compared with normal cells. In addition, an enhanced growth rate and loss of contact inhibition were observed in gynecologic cancer cells compared with normal cells (doubling times of 16.6 hr vs. 26 hr, respectively). The expression level of ITGA5, an alpha-5 integrin marker, was upregulated in normal myometrial cells, but this tendency was not exhibited in cervical cancer cells. Furthermore, p53 tumor suppressor gene expression was upregulated, whereas TLR4 and caspase-3 gene expressions were downregulated in cervical cancer cells. Notably, the expression levels of TLR4 and caspase-3 were increased significantly in LPS-treated cancer cells compared with those in non-LPS-treated cells. These results suggest that the TLR4-mediated caspase-dependent apoptotic signaling pathway could be suggested as a therapeutic target for the treatment of gynecologic cancers, including cervical cancers.

환자이동과 간호업무와의 관계에 관한 연구

박선화,김경순,박수경,이영미,정문희 한국보건통계학회 2002 보건정보통계학회지 Vol.27 No.1

This study is focused on understanding the relation between patient flow and nursing service. Investigated the number of patient and nurse who were under nursing service, after looking into the nursing list concerning the patient who were hospitalized at the mixed surgery unit and the sole surgery unit among the surgery unit in H hospital, one of Third Medical Institution in Seoul, from 1 January to 30 June, 2002. The result of study are as follows: 1. The mixed surgery unit is three times more than the sole surgery unit concerning the absolute number of admission and discharge's average in a day. 2. The factor of administration (transferring a room and unit, admission and discharge) and medical treatment (surgery), all of them that the mixed surgery unit is more than the sole surgery unit. This difference had the meaning statistically. 3. The number of patient per nurse is not different between the mixed surgery unit and sole surgery unit, however in case of the number of patient caused by movement factor, the mixed surgery unit is more than the sole surgery unit in a day and month. Consequently, it suggests to examine the working plan flexibly in a day and month with considering the administration factor such as movement, as thinking over the manpower arrangement in the unit with having the limited manpower.

消費者保護와 消費者行政

제미경,박수경 인제대학교 1992 仁濟論叢 Vol.8 No.1

The purpose of this study is to propose the planes for improving the Korean consumer administration more efficiently. For this study, the writer reviewed the present systems and actual situations of the consumer administrative organizations and legislation in Korea. The conclusions are as follows. As regards to the consumer administrative legislation, first, new regulatiosn and modifications to existing laws have to be adopted for preventing danger and injury, for reasonable measuring, labeling and contracting relations and for free competition of enterprise. Second, consumer education in school as well as in society has to be enforced by administrative and institutional support of the government. Finally, consumer organizations have to be brought up by administrative and financial support of the government. As regards to the consumer administrative organizations, first of all, a special consumer administrative organization which has holistic viewpoint about consumer protection, and which makes a plan of consumer policy and controls it has to be set up. And the organizations for consumer education and the systems for accepting the consumer complaints and testing the goods and services which consumers buy have to be set up around a wider area and the activities of those should be strengthened.