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Identification of Two Superoxide Dismutases (FeSOD and NiSOD) from Streptomyces peucetius ATCC 27952
Kanth, Bashistha Kumar,Oh, Tae-Jin,Sohng, Jae-Kyung 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.5
In this study, the whole genome of Streptomyces peucetius ATCC 27952 was analyzed and two superoxide dismutases (SODs), named sp-sod1 and sp-sod2, were identified. The sp-sod1 is a putative Fe-Zn sod that is 636 bp in length. The sp-sod2 is a putative NiSOD that is 396 bp in length. The deduced amino acid sequence of sp-sod1 shared approximately 85 ~ 90% identity with the iron sod of S. griseus, S. coelicolor A3(2), and S. avermitilis MA-4680 whereas sp-sod2 shared approximately 87 ~ 94% identity with S. avermitilis, S. coelicolor A3(2) and S. seoulensis. The sp-sod1 was characterized to be FeSOD in the sod mutant E. coli QC871. The N-terminal deleted sp-sod2 along with a putative signal peptidase sp-sodX, which was immediately downstream, was co-expressed in E. coli. This recombinant E. coli strain did not produce the processed mature Sp-SOD2 unlike S. coelicolor Muller. However, Sp-SOD2 was confirmed to be NiSOD in S. lividans TK24.
Streptomyces peucetius ATCC 27952 에서 Cytochrome P450 CYP107P3 의 컴퓨터 모델링
바시스터 水原大學校 2016 論文集 Vol.30 No.-
Streptomyces peucetius ATCC 27952 에서 CYP107P3는 7-ethoxycumarin를 putidaredoxin reductase 및 putidaredoxin의 합성으로 E. coli 안에서 7 hydroxycumarin으로 변환한다. CYP107P3의 동성 모델 은 템플릿 1EGY (CYP107A1), CV9 (CYP105A1), 2VZ7 (CYP107L1) 및 2WM4 (CYP124) 를 선택에 의 해 만들어진. 1EGY (CYP107A1), 3CV9 (CYP105A1), 2VZ7 (CYP107L1) 및 2WM4 (CYP124) CYP107P3 와 35 %, 34 %, 38 %, 36 %의 정체성 각각을 공유한다. 그 모델 7-ethoxycumarin과의 상호 작용을 연구하는데 사용될 수 있도록 모델화 CYP107P3는 품질 평가했다.
Kaur Chetan,Kanth Bashistha Kumar,이가영,KUMARI SHIPRA,이긍주 한국식물생명공학회 2022 Plant biotechnology reports Vol.16 No.6
New Zealand spinach (Tetragonia tetragonioides) is a coastal plant species with a variety of medicinal uses in Korea. Due to its salt-tolerant capabilities, land reclamation using coastal plants such as Tetragonia have been widely employed. So far, the information about the dynamics or diferentially expressed genes related to salt response in New Zealand spinach (NZS) is scarce. We analyzed the expressed sequence tags of the seawater-treated NZS, to identify key genes and pathways involved in salt tolerance. Our results indicated that the salt responsive DEGs were related to ion transport, signal transduction and secondary metabolite synthesis. Further analysis of the transcriptome profle of NZS subjected to seawater treatment highlighted the roles in scavenging and abscisic acid signal transduction. The DEGs, regulating pectin remodeling and ROS scavenging may be the key genes for NZS to adapt to salinity environment. Furthermore, genes such as senescence-associated genes (SAGs) that are highly upregulated in glycophytes during salt stress were downregulated in NZS. Similarly, during abiotic stresses the transcription of genes involved in photosynthesis is severely reduced, but the transcripts of light responsive factors in NZS were slightly downregulated, showing the efciency of the salt regulation mechanism in NZS. This study represents the frst large-scale transcriptome analysis of New Zealand spinach. Elucidating the salt tolerant properties of halophytes can provide novel fndings to enhance the salt sensitive plants. Our fndings could further help the understanding of the stress tolerance mechanisms in plants in general and New Zealand Spinach can be used as a halophytic model in stress-tolerance studies.
백승필,전소영,( Bashistha Kumar Kanth ),민기하,이창현 한국공업화학회 2014 한국공업화학회 연구논문 초록집 Vol.2014 No.1
Carbonic anhydrase (CA) has recently been considered as an efficient biocatalyst applicable for CO<sub>2</sub> capture process. We constructed codonoptimized sequence of PP-zCA and set up its expression/purification system using E. coli host. Subsequently, we characterized various properties of novel PP-zCA. PP-zCA was most active at ~50°C and pH 8.0 in 50 mM Tris/SO<sub>4</sub> buffer, and retained 70 % of its activity after incubation at 70°C for 15 min. We also demonstrated that PP-zCA enzyme can catalyze the conversion of CO<sub>2</sub> to CaCO<sub>3</sub>. SEM image revealed rhombohedral calcite crystals. XRD analysis of precipitated CaCO<sub>3</sub> indicates formation of a calcite phase. The 2θ diffraction peaks occurred at 29.5, 36.1, 39.5, and 43.3°, corresponding to the calcite crystal faces (104), (110), (113), and (202), respectively and the representative crystal surface of calcite was 2θ = 29.42.
Kumari, Shipra,Kanth, Bashistha Kumar,Jeon, Yongsam,Jang, Ji-Young,Kim, Hyun-Soon,Lee, Geung-Joo Elsevier 2018 Scientia horticulturae Vol.236 No.-
<P>We have developed a cleaved amplified polymorphic sequence (CAPS) marker with the aim of identifying the best species among the different wild Lilium species native to Korea for breeding purpose. The CAPS marker is based on single nucleotide polymorphisms (SNPs) in the nucleotide sequences of the internal transcribed spacer genes, which are widely used in the breeding of angiosperms. We cloned and sequenced the ITS regions of nuclear ribosomal genes from 11 Lilium species and deduced the phylogenetic relationships among them. Phylogenetic analysis based on the maximum likelihood method showed that Lilium species formed a monophyletic Glade and were divided into two groups that linked to the two sections, Martagon and Sinomartagon, out of seven sections of Lilium. The lengths of the ITS1 and ITS2 regions in the wild native Lilium species were distinct, and nucleotide polymorphisms were distinguished in these regions. A conserved region in the sequence was found from position 330-340 in all the species. We analyzed the SNP sites and found a suitable restriction endonuclease, Eco52I, having six base pair recognition site, to be suitable for the development of the CAPS marker. Among the 11 Lilium species, only the amplicon from the ITS region of L. hansonii contained the restriction site and produced two distinct bands of around 623 and 268 bp upon digestion with Eco52I. The genotyping of the 11 wild Lilium species by the developed marker can be recommended for breeding programs as it provides an indirect selection of plants and can effectively differentiate wild native lilies. The method using CAPS marker is simple, quick, and highly reliable for identifying the best species for efficient breeding of Lilium.</P>