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광주지역 소 생고기 선호도 및 유통단계별 세균학적 분석
나호명 ( Ho Myung Na ),배성열 ( Seong Yeol Bae ),고바라다 ( Ba Ra Da Koh ),장미선 ( Mi Sun Jang ),성창민 ( Chang Min Sung ),김지연 ( Ji Yeon Kim ),박헌규 ( Heon Gyu Park ),문용운 ( Yong Un Mun ),김용환 ( Yong Hwan Kim ) 한국동물위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.4
Consumer`s preference and microbial inspections on fresh raw beef were carried out to understand the actual market status in Gwanju, Korea. Over 15 questions on questionnaire by 1,111 randomly selected respondents between April and May in 2011, results showed 65.5% positive on eating fresh raw beef, 63.8% negative on good hygiene condition of fresh raw beef, and 72.5% positive on the secure of the hygiene-safety for priority program, respectively. For microbial inspections, a total of 302 samples were collected from fresh raw beef purchased from slaughterhouse (n=122), transport (n=69) and consumer (n=81) stage, from lettuce (n=30) at consumer stage. The aerobic plate count (APC), E. coli count and food borne bacteria such as Salmonella spp., Listeria monocytogenes, Staphylococ-cus( S.) aureus and E. coli O157:H7 were tested in the samples. As results, the level of count on APC of fresh raw beef ranged 6×10(1)∼1.8×10(5) CFU/g from slaughterhouse, 2×10(2)∼8.3×10(5) CFU/g from transport stage and 1×10(2)∼4×10(5) CFU/g from consumer stage. The level of count on E. coli of fresh raw beef ranged 1∼9×10(1) CFU/g from slaughterhouse, 1∼7×10 CFU/g from transport stage and 1∼5.5×10 CFU/g from consumer stage. In total, 26 S. aureus were isolated, 10 (14.5%) from fresh raw beef at transport stage, 12 (14.8%) from fresh raw beef and 4 (13.3%) from lettuce at consumer stage. Enterotoxin of S. aureus was not detected among 26 isolates. All S. aureus isolates were typed using a DiversiLab(TM) rep-PCR system for genetic similarity test, showing over 95% of genetic relationship amon isolates.
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소 림프절에서 Mycobacterium bovis DNA의 신속 검출과 M. bovis와 M. tuberculosis 감별을 위한 real-time PCR 개발
고바라다 ( Ba Ra Da Koh ),장영부 ( Young Boo Jang ),구복경 ( Bok Kyung Ku ),조호성 ( Ho Seong Cho ),배성열 ( Seong Yeol Bae ),나호명 ( Ho Myung Na ),박성도 ( Seong Do Park ),김용환 ( Yong Hwan Kim ),문용운 ( Yong Un Mun ) 한국가축위생학회 2011 韓國家畜衛生學會誌 Vol.34 No.4
Mycobacterium bovis, a member of the M. tuberculosis complex (MTC), is the causative agent of bovine tuberculosis. Detection of M. bovis and M. tuberculosis using conventional culture- and biochemical- based assays is time-consuming. Therefore, a simple and sensitive molecular assay for rapid detection would be of great help in specific situations such as faster diagnosis of bovine tuberculosis (bTB) infection in the abattoirs. We developed a novel multiplex real-time PCR assay which was applied directly to biological samples with evidence of bTB and it was allowed to differentiate between M. bovis and M. tuberculosis. The primers and TaqMan probes were designed to target the IS1081 gene, the multi-copy insertion element in the MTC and the 12.7-kb fragment which presents in M. tuberculosis, not in the M. bovis genome. The assay was optimized and validated by testing 10 species of mycobacteria including M. bovis and M. tuberculosis, and 10 other bacterial species such as Escherichia coli, and cattle lymph nodes (n=113). The tests identified 96.4% (27/28) as M. bovis from the MTC-positive bTB samples using conventional PCR for specific insertion elements IS1081. And MTC-negative bTB samples (n=85) were tested using conventional PCR and the real-time PCR. When comparative analyses were conducted on all bovine samples, using conventional PCR as the gold standard, the relative accuracy of real-time PCR was 99.1%, the relative specificity was 100%, and the agreement quotient (kappa) was 0.976. The detection limits of the real-time PCR assays for M. bovis and M. tuberculosis genomic DNA were 10 fg and 0.1 pg per PCR reaction, respectively. Consequently, this multiplex real-time PCR assay is a useful diagnotic tool for the identification of MTC and differentiation of M. bovis and M. tuberculosis, as well as the epidemiologic surveillance of animals slaughtered in abattoir.
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