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Pseudonocardia autotrophica 유래의 신규 Cytochrome Cytochrome P450 Hydroxylase 유전자의 분리 및 염기서열 특성규명
명지선,박현주,한규범,김상년,김응수,Myeong Ji Seon,Park Hyun-Joo,Han Kyuboem,Kim Sang-Nyun,Kim Eung-Soo 한국미생물학회 2004 미생물학회지 Vol.40 No.3
Novel cytochrome P450 hydroxylase (CYP) genes were isolated and characterized from P. autotrophica cosmid DNA library using an actinomycete CYP-specific PCR product as a screening probe. The cosmids containing four unique CYP genes (pESK601, 602, 603, 604, 605) were identified, and the four CYP genes were completely sequenced to be homologous to other known Actinomycetes CYP genes involved in various secondary metabolic pathways. Among all novel actinomycete CYP genes found in P. autotrophica, the CYP genes present in pESK601 were revealed to be highly homologous to the CYP genes involved in polyene-type amphotericin and nystatin antibiotic biosynthesis. The nucleotide sequences of the CYP flanking region in pESK601 also revealed the polyene-type biosynthetic genes, implying the presence of a cryptic polyene-type antifungal biosynthetic gene cluster in P. autotrophica. 본 논문에서는 희소 방선균, Pseudonocardia autotrophica(KCTC 9441) 유래 신규 Cytochrome P450 hydroxylase(CYP) 유전자들을 분리하여 염기서열 특성을 규명하였으며, 기존에 밝혀진 다른 방선균 유래 CYP 유전자들과의 연관성을 알아보았다. 이를 위하여 P. autotrophica의 cosmid DNA library를 제작하였고, 방선균에서 발견되는 CYP 유전자군의 보존된 서열로부터 제작된 degenerate primers를 이용한 PCR을 수행하여, P. autotrophica cosmid DNA library를 검색하였다. P. autotrophica cosmid DNA library검색 결과, P. autotrophica에는 염기서열이 서로 다른 4종의 신규 CYP유전자가 존재함이 확인되었으며 (CYP601-1, 601-2, 602, 605), 이들 신규 CYP유전자들은 방선균 유래 2차대사산물의 생합성에 관여하는 CYP유전자와 높은 유사성을 나타냈다. 특히, pESK601에서 확인된 CYP 유전자 및 주변 유전자의 염기서 열을 검색한 결과, polyene 계열의 항진균제, amphotericin과 nystatin의 생합성 유전자들과 매우 높은 유사성을 보임으로써, P. autotrophica에는 신규polyene계열의 항진균제 화합물의 생합성 유전자 군이 존재함도 규명되었다.
카이로프랙틱 경추교정이 대학생들에 경추배열과 경부통증 미치는 영향에 대한 연구
명지선,함주현 보완대체의학연구소 2010 Research Journal of Complementary and Alternative Vol.- No.1
This study aims to discover the influences of different treatments: Chiropractic manipulative therapy or Mackenzie neck exercise for 8 weeks for groups of university students who complain about cervical pain. The targets of this study are students in Hanseo university located in Seosan. They are divided into two groups of 16 people. The result below was obtained from Chiropractic being performed once every week for 8 weeks or Meckenzie neck exercise for 7 seconds 10 times in a row as 2 sets for 8 weeks. Cervical lordosis angle of x-ray was increased. Apart from measurement 2, measurement 1 and 3 showed significant differences (p < 0.05). The range of motion of cervical lordosis was shown increasing significantly (p < 0.05). Visual analogue scale was shown decreasing significantly (p < 0.05). Cervical dysfunction index was shown decreasing significantly (p < 0.05) According to the result above, it has a positive influence on the cervical spine. Therefore we have proven the grounds for applying chiropractic treatment and Mckenzie neck exercise and think they will help university students with cervical pain rehabilitate and improve functionally.
조수경,김형영,명지선,남은우,정선영,장은진,유대현,성윤경 대한의학회 2019 Journal of Korean medical science Vol.34 No.8
Background: This study aimed to estimate the incidence and prevalence of idiopathic inflammatory myopathies (IIM) and associated comorbidities in Korea from 2006 to 2015. Methods: IIM between 2004 to 2015 were identified using the Korean National Health Insurance Service medical claim database. The case definition required more than one visit based on diagnostic codes including juvenile dermatomyositis (JDM), dermatomyositis (DM), or polymyositis (PM) and registration in the Individual Copayment Beneficiaries Program (ICBP) for rare and intractable diseases. IIM patients with a disease-free period of 24 months before the index date were defined as incident cases. The Elixhauser comorbidity score was calculated. Results: Using the base case definition, 1,150 prevalent patients with IIM (117 JDM, 521 DM, 512 PM) were recorded in 2006 and 2,210 (130 JDM, 1,101 DM, 869 PM) in 2015. The prevalence was estimated at 2.3–4.0 (0.9–1.2 for JDM, 1.2–2.7 for DM, 1.4–2.1 for PM)/100,000 person-year (PY). We identified 218 incident cases of IIM in 2006 (18 JDM, 98 DM, 102 PM) and 191 cases (7 JDM, 83 DM, 101 PM) in 2015. The incidence was estimated at 2.9–5.2 (0.7–1.9 for JDM, 1.8–4.0 for DM, 1.6–3.0 for PM)/1,000,000 PY. The mean age (± standard deviation) of prevalent patients with IIM was 51.2 (± 16.9) years, and the percentage of women was 72.1%. More than two-thirds of patients (70.7%) had more than two comorbidities. Twenty percent of patients had interstitial lung diseases. Conclusion: In Korea, the incidence and prevalence of IIM were 2.9–5.2/1,000,000 PY and 2.3–4.0/100,000 PY, respectively