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Fungal Elicitor와 Exo-phytohormone이 청양고추(Capsicum annuum L.) 종자발아와 2차 대사산물에 미치는 효과
진한솔 ‧ 전우성 ‧ 김성재 ‧ 맹대현 ‧ 박세익 ‧ 이인순 ‧ 박흠대 대구대학교 산업기술연구소 2018 産業技術硏究 Vol.29 No.2
We studied growth rate and synthesis of secondary metabolite as capsaicin and polyphenolic compound during seed germination of Capsicum annuum L. on condition media with fungal elicitor and exo-phytohormone. The condition media was consisted MS (Murashige & Skoog) medium and sucrose for carbon source with different concentration of elicitor and exo-phytohormone. The fungal elicitor was made by Aspergillus niger KCCM 11240 and exo-phytohormone used kinetin, IAA (indole-3-acetic acid). The secondary metabolites were extracted from the seeding tissue by acetone as solvent after 20 days. As a result, the germination of seed was found on the third day and growth of seeding was observed on the seventh day. The capsaicin concentration was had the highest concentration in R0’’ (no exo-phytohormone, 180 mg/ml of fungal elicitor) extract among all extract solutions. The polyphenolic compound was had high concentration in R1’’ (0.1 mg/L of IAA and kinetin, 180 mg/ml of fungal elicitor) extract. The inhibition rate of lipid peroxidation was showed in proportion to the concentration of capsaicin. The effect of NO radical scavenging was measured more activity to natural pepper fruit than seeding cultured in vitro and that activity considered to be phytonutrient included capsaicin and polyphenolic compound. The fungal elicitor inhibited growth and differentiation of seeding, but it induced the synthesis of secondary metabolites. The secondary metabolites were in inverse proportion to total length of seeding. Therefore, capsaicin as phytonutrient can mass-produce by plant tissue culture with biotic stress.