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노영선 ( Young Sun Roh ),윤여백 ( Yea Baek Yoon ),김영진 ( Yong Jin Kim ),이성희 ( Seong Hee Rhee ),레브코로지스키 ( Lev Kolodzieyski ),임병무 ( Byung Moo Rim ),임채웅 ( Chae Woong Lim ) 한국가축위생학회 2001 韓國家畜衛生學會誌 Vol.24 No.2
To examine the prevalence and types of kidney lesions In Korean cattle, a survey was carried out at a abattoir in September 2000 and January 2001. The collected kidneys were examined grossly and histopathologically and investigated for the patterns of renal diseases with considering season and sex. Of 735 cattle (99 bulls and 246 cows in September and 140 bulls, 3 steers, and 247 cows in January) surveyed, 301 (41%) cattle had various renal lesions. The most common prominent finding was focal or multiple interstitial nephritis (36.2%). Other lesions included focal or multiple renal cyst (9.1%), infarction <0.1%), hemorrhage (0.3%), and renal atrophy (0.3%). Microscopically, main pathological lesions were acute or chronic interstitial nephritis with moderate infiltration of neutrophil or lymphocyte, respectively. Finally, the kidney lesions are more or less correlated with the meat quality, especially in female Korean cattle.
어린 shepherd 개에서 요독증의 조직 병리학적 연구
노영선 ( Young Sun Roh ),이승옥 ( Seung Ok Lee ),임채웅 ( Chae Woong Lim ),권오덕 ( Oh Deog Kwon ),윤여백 ( Yea Back Yoon ),이희문 ( Hee Moon Lee ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.22 No.4
A 7 month old male shepherd was presented with anorexia, diarrhea, and salivation, and euthanized due to no clinical improvement. Grossly, ulcers were seen on ventral surface of tongue and stomach. The mineralization was obvious in the intercostal space, lung and inner surface of aorta. Kidney was pale, firm and irregular. Histopathology confirmed uremic pneumonitis, end stage kidney and hyperplasia of parathyroid. This case was the typical uremic condition of juvenile dog, which was characterized the diffuse soft tissue mineralization due to hyperparathyroidism secondary to chronic renal failure.
HPLC/MS을 이용한 aminoglycoside 항생제동시 분석에 관한 연구
노영선 ( Young Sun Roh ),백귀정 ( Gui Jung Baek ),김승용 ( Seung Yong Kim ),최은영 ( Eun Young Choi ),서형석 ( Heyng Seok Seo ),허부홍 ( Boo Hong Hur ),정동석 ( Dong Suk Joung ) 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.1
A liquid chromatographic method has been developed for the analysis of aminoglycoside antibiotics(AMGs) using Heptafluorobutyric acid(HFBA) as a ion-pairing reagent. AMGs(amikacin, apramycin, dihydrostreptomycin, gentamicin, hygrosin B, kanamycin, neomycin, spectinomycin and tobramycin) were formed by reaction with HFBA as ion-pairing reagent. HFBA was attached to corresponding amino group of AMGs. These AMGs compounds were separated and detected by electrospray ionization mass spectrometry(ESI-MS). The experimental conditions for separation of AMGs were optimized and validated. A simple liquid chromatographic method for the determination of AMGs was demonstrated.
유산균 발효유 및 starter에서 항생제내성 유산균 분리 및 전달성 R plasmids 분포조사
백귀정 ( Kui Jeong Baek ),서형석 ( Heyng Seok Seo ),노영선 ( Young Sun Roh ),양해동 ( Hae Dong Yang ),허부홍 ( Boo Hong Hur ),서이원 ( Lee Won Seo ),정동석 ( Dong Suk Joung ),송희종 ( Hee Jong Song ) 한국가축위생학회 2006 韓國家畜衛生學會誌 Vol.29 No.3
Plasmids are covalently closed circular molecules of DNA that are stably inherited and replicate somewhat independently of the bacterial chromosome. Genes carried on plasmids can mediate a wide variety of important functions, including antibiotics(R plasmids) and heavy metals resistance, toxins production, cell penetration, iron chelation, complement resistance, and metabolic characteristics such as sucrose and lactose fermentation. Fifty strains of lactobacilli were isolated from 26 staters and 29 fermented milk products. They were classified 27 strains as Lactobacillus paracasei subsp paracasei , 11 stains as Lactococcus lactis subsp cremoris, 6 strains as L delbrueckii subsp lactis, 4 strains as L acidophius, and 2 strains as L delbrueckii subsp bulgaricus. All of these strains were examined for drug resistance and transferability of R plasmids. All of the isolates were sensitive to Am, C, CF, E, NB, P, T, and Te. But resistant to SXT 94%(47 strains), K 66%(33 strains), S 56%(28 strains), ENR 50%(25 strains), NOR 38% (19 strains) CIP 38%(19 strains), GM 16%(8 strains), and N 14%(7 strains), in order. And 32 different resistant patterns were found. The most frequently encountered patterns were CIP-ENR-K-NOR-S-SXT (5 strains). In vitro R plasmids transfer experiment, 57 antibiotic resistant strains which were not trans-fer to the recipient 2 Escherichia coli strains by conjugation. These results indicate that Lactobacillus in internal trade market` stater recognize R factor but transmissible R plasmid is not existed.