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HPLC/MS을 이용한 aminoglycoside 항생제동시 분석에 관한 연구
노영선 ( Young Sun Roh ),백귀정 ( Gui Jung Baek ),김승용 ( Seung Yong Kim ),최은영 ( Eun Young Choi ),서형석 ( Heyng Seok Seo ),허부홍 ( Boo Hong Hur ),정동석 ( Dong Suk Joung ) 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.1
A liquid chromatographic method has been developed for the analysis of aminoglycoside antibiotics(AMGs) using Heptafluorobutyric acid(HFBA) as a ion-pairing reagent. AMGs(amikacin, apramycin, dihydrostreptomycin, gentamicin, hygrosin B, kanamycin, neomycin, spectinomycin and tobramycin) were formed by reaction with HFBA as ion-pairing reagent. HFBA was attached to corresponding amino group of AMGs. These AMGs compounds were separated and detected by electrospray ionization mass spectrometry(ESI-MS). The experimental conditions for separation of AMGs were optimized and validated. A simple liquid chromatographic method for the determination of AMGs was demonstrated.