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나한식,조경상,김종두,이승일,문철웅,이호영,장숙진 朝鮮大學校 附設 醫學硏究所 1989 The Medical Journal of Chosun University Vol.14 No.2
Histiocytic medullary reticulosis is a clinicopathologic syndrome characterized by its acute onset and relentless progression to death within a few months. The major clinical features are fever, malaise, weakness, weight loss, lymphadenopathy, hepatosplenomegaly, jaundice and purpura. The common laboratory findings are severe anemia, leukopenia, and thrombocytopenia. Histologically, bone marrow were infiltrated with atypical, neoplastic histiocytes, many of which had ingested large number of red blood cells, leukocyte, platelet. We experienced one case of HMR who was a 22 year old male admitted via ER with malaise, general weakness, fever and headache. The patient died often 11 days of illness. Death was due to massive bleeding and DIC thought to be due to pancytopenia secondary to massive blood element phagocytosis. So, we reported one case which considered compatible for HMR, with a few elementary reviewed literatures.
Choi, Cheol-Hee,Kim, Hyang-Suk,Lee, Tae-Bum,Sue, Youn-Koung,Rha, Han-Sik,Jeong, Jae-Hwan,Lee, Hong-Young,Do, Nam-Yong,Park, You-Hwan,Min, Young-Don 조선대학교 부설 의학연구소 2000 The Medical Journal of Chosun University Vol.25 No.1
목적: 항암제에 대한 다약물내성의 출현이 임상 화학요법시 실패의 주된 원인중의 하나로 생각되고 있다. 최근 많은 내성 암세포에서 다약물내성관련단백(multidrug resistance-associated protein, MRP)이 다약물내성 기전으로 보고되고 있어, 이 단백의 유전자 발현의 분석이 절실히 요구되고 있다. 그러나 현재까지 보고된 MRP mRNA를 측정하기 위한 역전사-중합효소 연쇄반응(reverse transcription-PCR, RT-PCR)은 여러가지 문제점을 가지고 있다. 그러므로 본 연구에서는 RT-PCR방법을 이용하여 MRP mRNA를 보다 편리하고 정확하게 정량할 수 있는 방법을 확립하고자 하였다. 재료 및 방법: MRP를 과발현하는 세포주인 HeLaJ2c내성세포와 이 세포의 야생형(wild type)인 HeLaS3세포를 모델세포로 이용하여 RT-PCR방법의 조건을 최적화하였다. 정량성을 높히기위하여 b-actin mRNA를 내인성 대조물질로 사용하였다. MRP와 b-actin mRNAs의 세포내 함량의 큰 차이를 극복하기 위해서 각각의 PCR 증폭 kinetics를 측정하여 증폭이 plateau에 도달하기전의 PCR cycle 수를 선택하였다. 감도와 정량성을 높이기 위해서 동위원소를 사용하였을 뿐 아니라 신속하고 간편하게 정량할 수 있도록 MRP와 b-actin의 PCR조건은 같으면서 PCR산물의 크기는 서로 다르도록 primer쌍을 고안하였다. 이 RT-PCR방법을 이용하여 여러 암세포주와 백혈병환자의 시료에서 MRP mRNA의 상대적 양을 측정하였다. 결과: 본 연구에서 RT-PCR방법에 의한 MRP mRNA측정결과 Northern blot분석결과와 유사하였으며 재현성과 감도가 우수하였다. 이 같은 RT-PCR 방법으로 다양한 암세포주 및 백혈병환자의 암시료에서 다양한 정도로 발현되는 MRP mRNA를 간편하게 정량할 수 있었다. 결론: 본 연구에서 개선된 RT-PCR방법은 정량성이 높고, 편리하며, 감도가 뛰어나고, 재현성이 높아 임상시료를 포함한 암세포에서 MRP mRNA를 정량하는데 유용하게 이용될 수 있을 것으로 생각된다.
위장관 ( 胃腸管 ) : 정상인에서 호기내 수소농도 측정법을 이용한 유당불내성률
김만우(Man Woo Kim),김평남(Pyoung Nam Kim),나한식(Han Sik Na),오재선(Jae Sun Oh),이승일(Seung Il Lee),박찬국(Chan Guk Park) 대한소화기학회 1990 대한소화기학회지 Vol.22 No.3
N/A In order to detect lactose intolerance in study groups, we have performed the lactose breath test, lactulose breath test, and oral lactose tolerance test. The 50 cases (28 men, 22 women) of this study were selected from healty adults without known disease and free from gastrointestinal symptoms. 1) In 50 subjects, there were 32 subjects who had the intolerant symptoms such as abdominal pain, flatulence and diarrhea. 2) All subjects with intolerant symptoms were more than 20 ppm in breath hydrogen. 3) In two subjects among 11 cases with less than 20 ppm in breath hydrogen by lactose breath test have not increased by lactulose breath test (<10ppm). 4) The prevalence of lactose intolerance in healthy adults was 82 percent. From the above results, it is concluded that lactose breath test offers an attractive, noninvasive way of diagnosing lactose intolerance.
소화성 궤양환자의 흡연여부에 따른 혈청 Pepsinogen 1 치의 변화
오재선(Jae Sun Oh),김평남(Pyung Nam Kim),나한식(Han Sik Na),김중남(Jung Nam Kim),문철웅(Chul Oong Moon),김만우(Man Woo Kim),양성훈(Sung Hun Yang) 대한내과학회 1990 대한내과학회지 Vol.39 No.4
N/A Serum group I Pepsinogen (sPG I) levels were measured by radioimmunoassay in 159 patients undergoing endoscopy, then all patients were classified by endoscopic diagnosis, gastric mucosal histology, and smoking habits. The results were as follows: 1) Both nonulcer dyspepsia and duodenal ulcer smokers had significantly higher sPG I levels than their nonsmoking controls (p<0.005). 2) The frequency distvibution of superficial gastritis in the nonulcer dyspcpsia group with smoking habits revealed significantly higher levels than that of the nonsmoking controls (p<0.0025). 3) Serum PG Iin nonulcer dyspepsia patients revealed significantly higher levels in patients with superficial gastritis but there were not related to cigarette smoking. 4) The relationship between the mean level of serum PG I and fundic mucosal histology in duodenal ulcer patients was found to be statistically insignificant, but the mean level of sPG I in the smoking group showed a higher value than that of the nonsmoking controis (p< 0. 05). 5) The frequency distribution of high serum PG I level (> 80 ng/ml) in duodenal ulcer smokers was higher than that of the nonsmoking control group (chi square test: p<0.). From the above findings, it is concluded that the smoking induced increase in sPG I in duodenal ulcer is proposed to release an augmented pepsin secretory capacity, which can have aetiologic significance in association between smoking and duodenal ulcer.