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이용욱,나승식,조성범,정지연,박성기 한국환경보건학회 1996 한국환경보건학회지 Vol.22 No.4
It was intended to investigate the effect of the microbiological kitchen hygiene such as dishclothes and scrubbers. The 8indicator organisms (standard plate counts, coliform, heterotroph, enterococcus, staphylococcus, heat-stable bacteria, psychrotroph, Pseudomonas aeruginosa) were detected highly in dishwaters, dishcloth and scrubber. Coliform and Staphylococcus aureus were appeared on dishcloth dominantly than the scrubber, and the scrubbers were intruded by hetrotrophs and psychrotrophs numerously than dishclothes. The germ-resistant sponge inhibited the growth of the most of test strain, and appeared the about 100% reduction rate after 24 hr, but did not affect Pseudomonas aeruginosa and P. fragi so typically after 24 hr. The anti-microorganism durability of germ-resistant sponge, treated with food soil, was maintained by 10 days, the early stage strain density was founded in 20 days, and the strains grew after 30 days.
혈액내 Lipoprotein(a) 농도의 결정요인에 관한 연구 : 서울시 일부 초, 중, 고교 교사들을 대상으로
최현오,이용욱,나승식,이후장 한국식품위생안전성학회 1998 한국식품위생안전성학회지 Vol.13 No.2
본 연구는 정상 성인에서 관상동맥질환의 위험인자로 알려진 혈액내 Lipoprotein(a)농도의 실태를 파악하고 이들 농도와 지질농도, 섬유소원, 공복혈당 및 일상생활요인과의 관계를 알아보고자 수행되었다. 연구대상은 서울지역 일부 초, 중, 고교 교사들로 의료보험 관리공단의 신검에서 건강상태가 양호하다고 판정받은 남자 80명, 여자 60명을 대상으로 혈액분석과 설문 조사를 1990년 11월 2일부터 11월 7일에 걸쳐 실시하였다. 혈액분석 결과에 따라 이 들을 정상군과 위험군으로 나누고 이들 군들 사이에서 Lipoprotein(a)농도와의 연관성을 알아보았다. 결과는 다음과 같다. 1. 전체 대상자들 중 관상동맥질환 유발의 위험수준인 30㎎/dL을 초과하는 대상자는 140명 중 37명으로 26.4%에 달했다. 대 상자들의 Lipoprotein(a) 평균농도는 남자는 15.94± 12.70 ㎎/dL,여자는 17.92±17.58㎎/dL로 성별간의 유의한 차이는 없었다. 2. 대상자의 연령에 따른 Lipoprotein(a)농도의 경우 연령간에 유의한 차이를 보였다(p<0.05). 3. 대상자의 혈액을 분석하여 각 항목에 대해 정상군과 위험군으로 나눈 후 Lipoprotein(a) 농도와 각 항목들간의 유의성을 살펴본 결과 1) Aspartate aminotransferase, Alanine aminotransferase와 Lipoprotein(a) 농도 사이에는 유의한 차이가 나타나지 않았다. 2) 공복혈당, 섬유소원과 Lipoprotein(a) 농도 사이에도 유의한 차이가 관찰되지 않았다. 3) 지질농도(총콜레스테롤, 고비중 지단백콜레스테롤, 저비중 지단백 콜레스테롤, 중성지방)와 Lipoprotein(a) 농도 사이에도 유의한 차이가 발견되지 않았다. 4) 일상생활요인과 Lipoprotein(a) 농도와의 경우 혈압, 비만도, 성별, 흡연 등에서는 유의한 차이가 나타나지 않았다. 그러나 육식 섭취, 음주, 염분 섭취의 정도에 따라서 이들과 Lipoprotein(a)농도 사이에 유의한 차이가 있는 것으로 나타났다(p<0.05). 5) 혈액분석 결과와 일상생활요소를 모두 통털어 Lipoprotein(a) 농도가 고려된 경우 나이와 육식섭취의 정도^(*)에 따라 정상군과 위험군에서 유의한 차이가 관찰되었다(p<0.05, p<0.01^(*)). ABSTRACT- The purpose of this study was to determine the association between blood lipids, fibrinogen, fasting blood sugar, lifestyle-related factors and lipoprotein(a). This study was per- formed with 140 healthy adults (male:80, female:60) among the teachers in primary, middle, and high school in Seoul in November 1996. Lipoprotein(a) concentration was measured with an enzyme-linked-immunosorbents assay(ELISA1, and the results showed that the distribution was highly skewed and the average concentrations of male and female were 16.94k 12.70 mg/dL and 17.92f 17.58 mg/dL, respectively. The percentage of subject with lipoprotein(a) concentrations higher than 30 mg/dL was 26.4%. Univariate analysis showed significant association between lipoprotein(a1 and age, alcohol, meat, and NaCl consumption (p<0.05). In multivariate analysis, lipoprotein(a1 correlated positively with age (p<0.05) and meat consumption (p<O.Ol). These data suggest that lifestyle-related factors may affect the lipoprotein(a1 concentration regarded as a risk factor of atherosclerosis disease. Key words 0 Lipids, fibrinogen, atherosclerosis, lipoprotein(a1, eholesterol
식품접촉물질에 부착된 Listeria monocytogenes의 증식 및 제거에 관한 연구
윤정희,고영림,나승식,이용욱 한국환경보건학회 2001 한국환경보건학회지 Vol.27 No.1
Microorganisms can attach firmly to food contact surface material and the resitance of adherent bacteria differ markedly from planktonic cells. Therefore, adherent cells are a potential contamination problem to the food preparation because of their high resistance. to sanitation and heat treatment. This study was carried out in order to investigate growth and decontamination of Listeria monocytogenes attached to stainless steel, glass and plastic. Listeria monocytogenes cells could attach to all types of surface at three temperatures after contact times for 24 hrs. The numbers of adherent cells were greater at higher temperatures, but not increased with incubation time. When recovery of adherent cells was investigated, after 24 grs, the numbers of adherent cells were about 10$^{7}$ , 10$^{10}$ , 11$^{11}$ at 4$^{\circ}C$, $25^{\circ}C$, 3$0^{\circ}C$ repectively. Planctonic cells decreased by 2 log cycles after exposure to the domestic sanitizer. Adherent cells showed high resistance to domestic sanitizers and that was dependent upon surface materials studied, being greatest on plastic followed by stainless steel and glass. Adherent cells were more resistant to heat treatment than planktonic cells. When adherent cells were exposed to the temperature of 5$0^{\circ}C$, 55$^{\circ}C$, 57.5$^{\circ}C$ for 10 min, their populations did not decrease significantly. When the temprature increased to 6$0^{\circ}C$, cells attached to all types of surfaces were completely inactivated for 10 min.
시판 식물성 식품의 저장온도 및 저장기간별 세균균속의 변화
박석기,이용욱,나승식 대한보건협회 2000 대한보건연구 Vol.26 No.2
Out of a total of 118 genera isolated from commercially distributed plant foods, 34 genera were identified and among them the predominant genera were Pseudomonas(28.8%) and followed by Bacillus(26.3%), Staphylococcus(11.0%). Out of 1345 isolates from commercially distributed plant foods stored at 10℃, 20℃ and 30℃ for 14 days, 25 genera were identified and Pseudomonas(30.3%), Bacillus(23.4%) and Staphylococcus(8.6%) were predominant. The predominant genus among 491 isolates isolated from commercially distributed plant foods stored at 10℃ was Pseudomonas(35.2%), followed by Bacillus(22.8%) and Staphylococcus(7.9%). The predominant genus among 419 isolates isolated from commercial plant foods stored at 20℃ was Pseudomonas(27.7%) followed by Bacillus(24.3%) and Staphylococcus(7.6%) and the predominant genus among 435 isolates isolated at 30℃ was Pseudomonas(27.4%) followed by Bacillus(23.2%) and Staphylococcus(10.1%). Consequently, Pseudomonas and Bacillus were the most influent bacteria for the storage time and temperature in the commercially distributed plant foods. When it was in the higher storage temperature, Bacillus was highly influenced, and in the lower storage temperature, Pseudomonas was influenced. But the longer the storage time was, the greater the hygienic influence of Bacillus, and the shorter the storage time was, the greater the hygienic influence of Pseudomonas was.