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김현명,강세병,정승호,Kim, Hyeon Myeong,Gang, Se Byeong,Jeong, Seung Ho Korean Chemical Society 2001 Bulletin of the Korean Chemical Society Vol.22 No.9
Thermoanaerobacter ethanolicus is a strictly anaerobic and thermophilic bacterium whose optimum temperature ranges over $65-68^{\circ}C.$ T. ethanolicus was known to contain a bipolar very long chain fatty acyl component such as $\alpha$, $\omega-1316-dimethyloctacosanedioate$, as one of the major membrane components. However, exact physiological role of this unusual component in the membrane remains unknown. Such a very long chain fatty acyl component, $\alpha$, ${\omega}-1316-dimethyloctacosanedioate$, dimethyl ester (DME C30), was isolated, and purified from the membrane of T. ethanolicus. As a function of added concentrations of the $\alpha$, $\omega-1316-dimethyloctacosanedioate$, dimethyl ester (DME C30) or cholesterol into the standard liposomes, the acyl chain ordering effect was investigated by the steady-state anisotropy with 1,6-diphenyl-1,3,5-hexatriene (DPH) as a fluorescent probe. Acyl chain order parameter (S) of vesicles containing DME C30 is higher comparing with phosphatidylcholine (PC) only vesicles. This result was discussed thermodynamically with the aid of the simulated annealing molecular dynamics simulations. Through the investigation of all the possible conformational changes of DME C30 or cholesterol, we showed that DME C30 is very flexible and its conformation is variable depending on the temperature comparing with cholesterol, which is rigid and restricted at overall temperature. We propose that the conformational change of DME C30, not the configurational change, may be involved in the regulation of the membrane fluidity against the changes of external temperature.
1913년부터 2013년까지 한국에서 육성된 콩 품종의 Isoflavone 함량의 변이와 다변량 분석에 의한 품종군 분류
김현명(Hyun Myung Kim),장은규(Eun Kyu Jang),곽병삼(Byeong Sam Gwak),황태영(Tae Young Hwang),윤건식(Geon Sig Yun),황세구(Se Gu Hwang),정헌상(Heon Sang Jeong),김홍식(Hong Sig Kim) 한국육종학회 2018 한국육종학회지 Vol.50 No.1
1913년부터 2013년까지 국내에서 육성된 장류용, 나물용, 풋콩 및 올콩용, 밥밑용 품종을 포함한 172 품종에 대한 종실의 isoflavone 12개 성분의 함량에 대한 변이를 구명하고, 다변량분석에 의한 품종군을 분류하였다. 종실의 총 isoflavone의 평균함량은 837.2 μg/g이었고, 206.3~2666.2 μg/g의 범위이었다. 성분별로는 malonylgenistin의 평균함량이 390.0 μg/g로서 가장 높았고, 99.2~1149.1 μg/g의 범위이었고, glycitein의 평균 함량이 0.8 μg/g로서 가장 낮았으며 0.1~2.4 μg/g의 범위이었다. 성분그룹별로 보면 malonylglucoside group의 평균함량은 599.1 μg/g로서 가장 높았고 143.6~1796.4 μg/g의 범위이었으며, glucoside group의 평균함량은 202.4 μg/g이었고 36.9~801.8 μg/g의 범위이었다. acetylglucoside group의 평균함량은 27.5 μg/g이었고 3.7~103.9 μg/g의 범위이었으며, aglycone group의 평균함량은 10.0 μg/g로서 가장 낮았고 2.1~33.1 μg/g의 범위이었다. 총 isoflavone함량이 높은 품종은 나물용 품종인 아가8호(2666.2 μg/g), 아가4호(2569.3 μg/g) 및 아가9호(2345.0 μg/g)이었고, 총 isoflavone 함량이 낮은 품종은 장류용 품종인 대왕(328.8 μg/g), 풋콩 및 올콩 품종인 한올(599.3 μg/g) 그리고 밥밑용 품종인 검정콩1호(664.1 μg/g)이었다. Isoflavone성분에 대한 주성분분석을 실시한 결과, 제 1~3주성분까지의 누적기여도가 89.14%이었고, 제 3주성분만으로도 한국 콩 품종의 군집분류가 가능하였다. 군집분류에서 평균거리를 0.8로 하였을 때 7개의 군집으로 분류되었고, Ⅰ군집은 전체 품종의 57.6%가 속하는 가장 큰 군집이었고, 다음으로 Ⅲ군집이 35.5%가 속하는 큰 군집이었으며, 나머지 5개 군집은 5개 이하의 품종이 속하는 소군집이었다. 군집에 따른 총 isoflavone의 평균함량은 Ⅵ군집이 2526.9 μg/g으로 가장 높았으며, 다음으로 Ⅳ군집이 1546.6 μg/g, Ⅴ군집이 1503.5 μg/g으로 높았으며, 그 외 군집은 낮았다. This study was carried out to investigate the variation of 12 isoflavone components among 172 Korean soybean varieties released from 1913 to 2013. Cluster analysis was performed to classify the varieties based on the variation of isoflavone contents. Total isoflavone contents ranged from 206.3 μg/g to 2666.2 μg/g with an average of 837.2 μg/g. By each component, malonylgenistin content ranged from 99.2 μg/g to 1149.1 μg/g with the highest average of 390.0 μg/g, and glycitein content ranged from 0.1 μg/g to 2.4 μg/g with the lowest average of 0.8 μg/g. Among composition groups, the aglycone group contents ranged from 2.2 μg/g to 33.1 μg/g with the lowest average of 10.0 μg/g, and glucoside group contents ranged from 36.9 μg/g to 801.8 μg/g with an average of 202.4 μg/g. The malonylglucoside group contents ranged from 143.6 μg/g to 1796.4 μg/g with the highest average of 599.1 μg/g and acetylglucoside group contents ranged from 3.7 μg/g to 103.9 μg/g with an average of 27.5 μg/g. Varieties with high total isoflavone content were Aga8(2666.2 μg/g), Aga4(2569.3 μg/g), and Aga9(2345.0 μg/g) in bean sprouts group. The low total isoflavone content were observed from Deawang(328.8 μg/g) in soy sauce and paste group, Hanol(599.3 μg/g) in vegetable and early maturity group and Gemjeong1(664.1 μg/g) in cooking with rice group. The first three principal component axises accounted for 89.14% of the total variation observed. Dendrogram constructed using average linkage cluster method revealed that 172 Korean soybean varieties were divided into seven groups with the average distance of 0.8 between groups. The group Ⅰ includes 57.6% of the total varieties, which was the largest group among seven groups, followed by the group Ⅱ that includes 35.5% of the total varieties. However, the other groups were small groups containing less than five varieties. In comparison among the total isoflavone contents according to groups, the highest isoflavone content was observed in group Ⅵ with an average of 2526.9 μg/g, followed by group Ⅳ(1546.6 μg/g) and group Ⅴ(1503.5 μg/g). The others were found to contain low isoflavone content.