란탄족 기반의 Upconversion 나노입자 합성 연구

김한비,김민섭,이상엽 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0

란탄족 이온이 도핑된 upconversion 나노입자는 낮은 에너지를 흡수해 높은 에너지를 방출하는 광학 시스템이다. 란탄족은 4f-4f 전이 메커니즘을 이용하여 단색광에 의한 여기가 가능하여 디스플레이나 센서 등에 사용할 수 있다. 기존의 유기염료와 퀀텀닷과 달리 적외선을 이용하여 가시광선을 낼 수 있으므로, 높은 광 안정성을 가지며 광원에 의한 생체 및 세포의 손상을 줄일 수 있다. 본 연구에서는 란탄족 이온을 도핑한 upconversion 나노입자에 관한 합성 및 성능 연구를 진행하였다. 합성된 나노입자는 980 nm의 적외선을 흡수해 도핑되는 이온의 농도에 따라 540 nm, 660 nm의 다른 가시광을 방출하는 것을 확인하였다. 이에 따라 해당 나노 입자의 광학 특징을 이용하면 센서, 바이오 이미징 및 암치료 등 다양한 분야에 활용될 수 있을 것으로 기대된다.

Label-free and multiplex detection of Alzheimer’s disease biomarkers using gold nanoplasmonic biosensor based on LSPR with colorimetric assay

김한비,양수지,심상준 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.1

Alzheimer’s disease (AD) is a neurodegenerative disease associated with the loss of nerve cell in areas of the brain. To diagnose AD on early pathological stage, many AD studies focus on the blood-based diagnostic test which is easy to obtain the sample and reflects a change of biomarkers from CSF due to the drawbacks of common tests. Among the rich sources in blood, Amyloid beta 1-42, Amyloid beta 1-40 and Tau protein, which are closely associated with key mechanism of AD pathology occurring in the brain, are able to be supportive early screening and diagnostic tools. Here, we developed the multi-detection biosensor of those markers based on Rayleigh scattering and colorimetric assay depend on plasmonic properties of AuNPs. Also, we utilized chaotropic agent which interfere the binding between biomarkers and other plasma protein to increase the detection limit. This is the first platform to detect AD biomarkers in blood, which can be used to diagnose AD directly in the future.

Multi-detection of Alzheimer’s disease biomarkers using nanoplasmonic biosensor based on LSPR with colorimetric assay

김한비,양수지,심상준 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0

Alzheimer’s disease (AD) is a neurodegenerative disease associated with the loss of nerve cell in areas of the brain. To diagnose AD on early pathological stage, many AD studies focus on the blood-based diagnostic test which is easy to obtain the sample and reflects a change of biomarkers from CSF due to the drawbacks of common tests. Among the rich sources in blood, Amyloid beta 1-42, Amyloid beta 1-40 and Tau protein, which are closely associated with key mechanism of AD pathology occurring in the brain, are able to be supportive early screening and diagnostic tools. Here, we developed the multi-detection biosensor of those markers based on Rayleigh scattering and colorimetric assay depend on plasmonic properties of AuNPs. Also, we utilized chaotropic agent which interfere the binding between biomarkers and other plasma protein to increase the detection limit. This is the first platform to detect AD biomarkers in blood, which can be used to diagnose AD directly in the future.

Upconversion nanoparticles를 이용한 H₂O₂ 검출 연구

김한비,이상엽 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.1

란탄족 이온이 도핑된 광에너지 상향전환 나노입자(upconversion nanoparticle, UCNP)는 낮은 광에너지를 흡수해 높은 에너지를 방출하는 시스템이다. 일반적인 자외선을 흡수하는 유기염료와 퀀텀닷과 달리 UCNP는 낮은 에너지를 갖는 적외선을 흡수하여 가시광선의 빛을 낼 수 있으며, 높은 광 안정성을 가지므로, 일반적인 자외선과 달리 광원에 의한 생체 및 세포의 손상을 줄일 수 있다. 본 연구에서는 cysteamine으로 개질된 UCNP 콜로이드 입자에 porphyrin을 결합하여, Forster resonance energy transfer (FRET) 기반의 H₂O₂ 감지 시스템을 제시했다. UCNP를 energy donor, 540 nm의 가시광 파장을 흡수하는 porpyrin을 energy acceptor로 사용하여, 광에너지 상향에 의한 발광 비율 변화를 관측하여 H₂O₂ 감지를 구현했다. 이 나노입자 시스템은 향후 적외선을 이용해 세포 손상 없이 세포 내에서 발현되는 H₂O₂를 감지할 수 있을 것으로 기대된다.

Detection of ApoE4-mediated beta amyloid42 aggregation based on localized surface plasmon resonance biosensor

김한비,강민경,심상준 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.1

Alzheimer’s disease (AD), which is the most common form of dementia, is characterized by a widespread functional disturbance of the human brain. From the statistical report in 2013, just over a tenth of people in the over-65 age group have the disease in United States. In the over-85s, the proportion goes up to about a third. Brain plaque deposition in the form of beta amyloid (Aβ) is a pathological hallmark of AD. The Aβ deposition is facilitated by an isoform of Apolipoprotein E4 (ApoE4), which is a dominant genetic risk factor of AD. Here, we present a nanoplasmonic biosensor to detect the dynamics of ApoE4-mediated Aβ aggregation. This sensor is based on the localized surface plasmon resonance (LSPR) of a single gold nanoparticle. In this sensor, ApoE4 is exploited as an inducer of Aβ42 aggregation. According to the LSPR spectra, the aggregation of Aβ42 is more specific and rapid than that of Aβ40. In addition, a detection limit of 50 μM for Aβ42 can be obtained corresponding to the 12.5 nm LSPR-peak shift, which is in line with the requirement for clinical detection. This is the first platform for the real-time detection of Aβ aggregation, mimicking the biological conditions, which can be used to investigate AD directly in the future.

MZ 세대 취향 맞춤형 알고리즘 서비스

김한비,남채령,유지희 한국서비스경영학회 2022 한국서비스경영학회 학술대회 Vol.2022 No.11

Multiplex Molecular Point‑of‑Care Test for Syndromic Infectious Diseases

김한비,허희재,박은경,정두련,강민희 한국바이오칩학회 2021 BioChip Journal Vol.15 No.1

Point-of-care (POC) molecular diagnostics for clinical microbiology and virology has primarily focused on the detection of a single pathogen. More recently, it has transitioned into a comprehensive syndromic approach that employs multiplex capabilities, including the simultaneous detection of two or more pathogens. Multiplex POC tests provide higher accuracy to for actionable decisionmaking in critical care, which leads to pathogen-specific treatment and standardized usages of antibiotics that help prevent unnecessary processes. In addition, these tests can be simple enough to operate at the primary care level and in remote settings where there is no laboratory infrastructure. This review focuses on state-of-the-art multiplexed molecular point-of-care tests (POCT) for infectious diseases and efforts to overcome their limitations, especially related to inadequate throughput for the identification of syndromic diseases. We also discuss promising and imperative clinical POC approaches, as well as the possible hurdles of their practical applications as front-line diagnostic tests.

Versatile sensor system for drug-discovery based on localized surface plasmon resonance

김한비,송소진,차미선,심상준 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.1

Signal transducer and activator of transcription 3 (STAT3) protein is well-known cytoplasmic transcriptions factor that regulatory functions in cell proliferation, differentiation and survival. In many cancers, STAT3 protein found over-activated form and it lead to uncontrolled expression of the STAT3 target genes. So activated STAT3 protein is consideration to be ideal targets for discovery of new anti-cancer therapy. Here, we first demonstrate the tracking of STAT3 signaling pathway such as phosphorylation, dimerization of STAT3 protein based on localized surface plasmon resonance (LSPR) spectra. Dramatic LSPR Δ λmax shifts were measured in the STAT3 signaling pathway steps and these signals were reduced by the treatment of inhibitor of STAT3 dimer. These results suggest that our system is able to effectively applicate for drug discovery for anti-cancer therapy.

Developing the Biosensor of ApoE4-mediated β-amyloid aggregation on plasmonic single nanoparticle

김한비,강민경,심상준 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.0

영화<글러브>에 나타난 청각장애학생선수들의 사회화경험

김한비,임수원,권기남 한국스포츠사회학회 2017 한국스포츠사회학회지 Vol.30 No.3

The purpose of this research was to find out the difficulties that those hearing-impaired student-athletes were having while playing baseball in a sports film, G-love and to have an idea of what kind of socialization they got to experience. In order to achieve it, the film, G-love, was chosen and text-analyzed and the results are as follows. This study selected the sports film <G-love> based on a true story. Date ware analyzed by textual analysis. Firstly, the difficulties that they had turned out to the trouble of learning, limits against their wills, discrimination into sports, and negative prejudices against them. Secondly, they formed their identities as athletes through baseball and challenged for their limits with confidence of doing so. Not only was the cohesion of the team strengthened by their learning to cooperate, but also they could break the stereotype that people had towards the disabled by showing how good they became, through hard training to become qualified athletes, against nondisabled student-athletes. 본 연구의 목적은 스포츠영화 <글러브>에서 청각장애학생선수들이 야구를 하면서 겪게 되는 어려움을 알아보고, 야구를 통해 어떠한 사회화를 경험하게 되는지 살펴보는 것이다. 이러한 연구목적을 달성하기 위해 <글러브>영화를 선정하여 텍스트 분석을 실시하였으며, 이를 통해 도출된 결과는 다음과 같다. 첫째, 청각장애학생선수들이 야구를 하면서 겪는 어려움으로는 운동습득의 어려움, 타의적 한계, 스포츠로 이어지는 차별, 사람들의 부정적인 편견인 것으로 나타났다. 둘째, 청각장애학생선수들은 야구를 통해 선수로의 정체성을 형성하고, 할 수 있다는 자신감을 갖고 자신이 가진 한계에 도전하게 되었다. 또 야구를 통해 협동심과 팀워크를 배우게 되면서 팀의 결속력이 강화되었고, 힘든 훈련으로 선수로서 기량을 갖추어 일반학생선수들과의 훌륭한 경기를 보여줌으로써 사람들이 가진 장애인에 대한 선입견이나 편견을 깨트려주었다.