고려인삼이 흰쥐의 장기조직 핵산 함유량에 미치는 영향

김철,최현,김정진,김종규,김명석,허만경,Kim, Chul,Choi, Hyun,Kim, Chung-Chin,Kim, Jong-Kyu,Kim, Myung-Suk,Huh, Man-Kyung 대한생리학회 1971 대한생리학회지 Vol.5 No.1

I. Chemical analysis A study was planned to see if administration of ginseng extract has any influence upon the adrenal, the hepatic, the splenic, and the pancreatic nucleic acid contents of rats, and to estimate the effect of ACTH administration as a substitute for stress reaction upon these nucleic acid contents of rats previously primed with ginseng. Ninety male rats$(body\;weight:\;150{\sim}200gm)$ were divided into the ginseng, the saline, and the normal control groups, which received for 5 days 0.5ml/100 gm body weight of ginseng extract solution (4 mg of ginseng alcohol extract in 1 ml of saline), same amount of saline, or no medication, respectively. On the 5th experimental day, each of the 3 groups was further divided into 2 subgroups yielding the ginseng, the ginseng-ACTIT, the saline, the saline-ACTH, the normal control, and the normal-ACTH subgroups. The ginseng, the saline, and the normal control subgroups were sacrificed 3 hours after the last medication, while the ginseng-ACTH, the saline·ACTH, and the normal-ACTH subgroups received ACTH(0.1 unit/subject) 1 hour after the last medication and were sacrificed after 1 more hour. The adrenal gland, the liver, the spleen and the pancreas of each rat were measured for RNA and DNA contents using the chemical method of Schmidt-Thannhauser-Schneider. Following results were obtained: 1. Adrenal RNA and DNA contents and RNA/DNA ratio were all significantly higher in the ginseng group compared with the values obtained from the normal control and the saline groups. Generally administration of ACTH reduced nucleic acid contents of the viscera examined. However, in the ginseng group the rate of decrease [(value of ginseng-ACTH subgroup-value of ginseng subgroup) x100/value of ginseng subgroup)] in adrenal RNA and DNA contents and in RNA/DNA ratio were more conspicuous than they were in the normal control and the saline groups. 2. Hepatic RNA and DNA contents and RNA/DNA ratio were all significantly less in the ginseng group than in the normal control and the saline groups. After ACTH, the rate of decrease in hepatic RNA, DNA, and RNA/DNA ratio of the ginseng· group was less conspicuous than those of the other 2 groups. 3. With regard to the splenic nucleic acid contents, the RNA and the RNA/DNA values of the ginseng group were higher than those of the normal control group but lower than those of the saline group, while the DNA value of the ginseng group was lower than that of the normal control group but higher than that of the saline group. Following administration of ACTH, the rate of decrease in RNA and DNA contents and in RNA/DNA ratio of the ginseng group was more conspicuous than that of the normal control group but less remarkable than that of the saline group. 4. Pancreatic RNA and DNA contents were notably lower in the ginseng group than in the normal control and the saline groups. However, the RNA/DNA ratio of the ginseng group was higher than that of the normal control and the saline groups.'After ACTH, the rate of decrease in pancreatic RNA and RNA/DNA ratio of the ginseng group was less than that of the normal. control group but more than that of the saline group, while the DNA content was actually increased in the ginseng group though it decreased in the normal control and the saline groups. Although the results are not clear enough for an accurate interpretation, they seem to indicate that ginseng exerts notable influence upon the RNA and DNA contents and the RNA/DNA ratio of the viscera stodied. On the whole the drug tends to increase the RNA and DNA contents and RNA/DNA ratio of the adrenal gland but seems to diminish the values of the other 3 viscera. In the early period following ACTH, ginseng facilitates the fall in RNA and DNA contents and RNA/DNA ratio of the adrenal gland, while it tends to reduce the fall in the values of the other viscera studied. II. Autoradiographic and histochemical analysis It was planned autoradiographically and his 1. 인삼주정추출물이 흰쥐의 부신, 간, 비장 및 췌장의 핵산 함유량에 어떤 영향을 미치며, 인삼 투여를 받고 있는 동물이 스트레스(ACTH의 투여로 대신)를 받을 경우에 이들 장기 핵산 함유량에 어떠한 변동이 오는지를 알기 위하여 90마리의 흰쥐($150{\sim}200gm$) 수컷을 인삼군과 식염수군 및 정상군으로 나누고 이것을 각각 다시 둘로 쪼개어 인삼군, 인삼-ACTH군, 식염수군, 식염수-ACTH군, 정상군 및 정상-ACTH군의 여섯 작은 무리로 나누어 다음과 같은 실험을 하였다. 인삼군과 인삼-ACTH군에서는 몸무게 100gm에 대하여 인삼주정추출액(생리적 식염수 1ml속에 4mg의 인삼주정추출물이 포함된 용액)을 0.5ml의 비율로 매일 등뒤 피하에 5일동안 주사하였으며, 식염수군과 식염수-ACTH군에는 생리적 식염수를 몸무게 100gm에 대하여 0.5ml의 비율로 인삼군과 동일한 방법으로 주사하였다. 실험시작 제 5일째에 정상군과 식염수군 및 인삼군은 각각 전처치 없이 또는 인삼 혹은 식염수 주사후 2시간 만에 도살하고, 정상-ACTH군, 인삼-ACTH군 및 식염수-ACTH군은 각각 직접 또는 인삼이나 식염수를 투여한 다음 1시간 만에 ACTH를 주사하고, 그 시각부터 1시간후에 도살하여 부신, 간, 비장 및 췌장 조직의 핵산 함유량을 측정하였다. 이들 측정치를 지표로하여 인삼이 장기 핵산에 미치는 영향을 관찰한바 다음과 같은 결과를 얻었다. 1) 부신조직 RNA, DNA 및 RNA/DNA는 인삼군의 값이 정상군 및 식염수군의 그것보다 현저하게 많다. 인삼군의 값에 대한 인삼-ACTH군 값의 감소율은 정상군 및 식염수군에 대한 정상-ACTH군 및 식염수-ACTH군의 그것보다 현저하게 크다. 2) 간 조직의 RNA, DNA 및 RNA/DNA는 인삼군의 값이 정상군 및 식염수군의 값보다 현저하게 감소되었다. 인삼군 값에 대한 인삼-ACTH군 값의 감소율은 정상군 및 식염수군에 대한 정상-ACTH군 및 식염수-ACTH군의 그것 보다 적다. 3) 비장 조직의 RNA와 RNA/DNA는 인삼군의 값이 정상군의 그것 보다 현저하게 증가되며, 식염수군 보다는 감소된다. 인삼군의 DNA는 정상군에서 보다 감소되며, 식염수군의 그것 보다 증가된다. 인삼군 값에 대한 인삼-ACTH군 값의 감소율은 정상군에 대한 정상-ACTH군의 그것 보다 크며, 식염수군에 대한 식염수-ACTH군의 그것 보다는 작다. 4) 췌장 조직의 RNA와 DNA는 인삼군의 값이 정상군 및 식염수군의 그것 보다 현저하게 감소된다. 인삼군의 RNA/DNA는 정상군 및 식염수군의 그것 보다 증가된다. 인삼군에 대한 인삼-ACTH군의 RNA와 RNA/DNA 감소율은 정상군에 대한 정상-ACTH군의 그것 보다 적으나 식염수군에 대한 실염수-ACTH군의 그것 보다는 현저하게 크다. 인삼-ACTH군의 DNA는 인삼군의 그것 보다 증가되었으나, 정상-ACTH군 및 식염수-ACTH군의 DNA는 정상군 및 식염수군의 그것보다 감소되었다. 이상의 실험성적만으로 명확한 해석을 내리기는 어려우나 인삼은 장기의 RNA, DNA 및 RNA/DNA 비율에 현저한 영향을 미치는 것으로 보인다. 대체로 인삼은 부신의 RNA와 DNA 함유량 및 RNA/DNA 비율을 모두 증가시키나 다른 장기에서는 이들 값을 감소시키는 경향이 있다. 한편 인삼의 투여를 받은 동물에서는 ACTH투여후 조기에 부신 조직의 RNA, DNA 및 RNA/DNA의 감소율이 커지며, 간, 비장 및 췌장 조직의 RNA, DNA 및 RNA/DNA의 감소율은 적어지는 경향이 있다. 2. 인삼 및 ACTH의 투여에 의하여 부신, 췌장, 간 및 비장의 DNA와 RNA 함유량이 변동하는 모습을 화학적 방법으로 추적하여 얻은 전편의 성적

대구,경북지역 요양보호사의 직무소진이 서비스 질에 미치는 영향

김철성 ( Chul Sung Kim ),조만복 ( Man Bok Jo ),김일범 ( Il Bum Kim ) 한국노인의료복지학회 2013 노인의료복지연구 Vol.5 No.1

This research showed that low-educational singles or divorced persons have a high job burnout according to general characteristics. And the worse health a person falls into, the higher job burnout a person gets by the difficulty of job performance. It is necessary to make job burnout low through responsible improvement of labor conditions. The improvement of appropriate job environment is required too because it showed that the long time work makes job burnout high. The systematic support is needed to maintain continuously the service with good quality because it showed that care workers with long-time work experience and good health are skilled in service. Also because it showed that care workers have usually provided the efficient service if the daily work hour is shorter, the long-time work should be avoided. The political support is required to provide the service with good quality through improvement of labor conditions because a person with the higher salary has usually provided the service of higher quality. According to the analysis result about the influence to service quality by job burnout, it was verified that burnout has an influence to the service quality. It showed that especially the lack of personal sense of accomplishment has a great effect to the service. Therefore the consolidation between workers and supporting welfare practice are really required to inspire personal sense of accomplishment of care workers through job condition improvement of facilities and emotionally stable work.

부식성장 예측모델을 이용한 가스배관의 신뢰도기반 구조건전성 평가

김익중(Ik Joong Kim),김철만(Chul Man Kim),김영표(Young Pyo Kim),김우식(Woo Sik Kim),최병학(Byung Hak Choe),김성준(Seong Jun Kim) 대한기계학회 2016 대한기계학회 춘추학술대회 Vol.2016 No.4

연성파손모델을 이용한 부식결함배관의 인장한계변형성능 평가

김익중(Ik-Joong Kim),장윤찬(Yun-Chan Jang),김철만(Chul-Man Kim),백종현(Jong-Hyun Baek),김영표(Young-Pyo Kim),김우식(Woo-Sik Kim),장윤영(Youn-Young Jang),허남수(Nam-Su Huh) 대한기계학회 2019 대한기계학회 춘추학술대회 Vol.2019 No.11

This paper presents a tensile strain capacity of API 5L X70 gas pipelines with corrosion defects using the ductile fracture model. Ductile fracture model was created based on H-M (Hancock-Mackenzie) model, and tensile tests were performed using notched tensile test specimen to produce fracture locus considering various stress triaxiality ratio. FE analysis results using the H-M model show overall good agreement with the tensile test result. Consequently, tensile limit strain capacity was performed on various corrosion defects pipe, and the consistency of the FE results was confirmed through comparison with the full-scale test results.

노인요양시설 요양보호사의 소진이 이직의도에 미치는 영향에서 조직몰입과 직무만족의 매개효과

김일범 ( Il Bum Kim ),조만복 ( Man Bok Jo ),김철성 ( Chul Sung Kim ) 한국노인의료복지학회 2013 노인의료복지연구 Vol.5 No.1

The result of this study is as follows. 386 workers were selected for this purpose, and there was a considerable difference in the intention of changing occupation according to the characteristics of sociology of population, such as age, marital status, and health. The group of younger age shows more intention of changing occupation; the unmarried group shows higher percentage to leave than the married group; and those who are in bad condition of health shows higher percentage than the healthy, which means that the management of their own health is very important. Conclusively, in order to reduce the number of nursing home workers to leave, a scheme to reduce the amount of work which helps reduce the exhaustion is required. Both the political consideration to increase job satisfaction and the surroundings to help organizational commitment are the two major factors which will reduce the number of leaving workers who are the necessary human power for the settlement of the successive long-term care insurance.

전단응력 상태를 고려한 고강도 강재의 파괴곡선 산출

김익중(Ik-Joong Kim),장윤찬(Yun-Chan Jang),김철만(Chul-Man Kim),허용화(Yong-Hwa Heo),선종오(Jong-Oh Sun),최신범(Shin-Beom Choi),정종하(Jong-Ha Jeong) 대한기계학회 2022 대한기계학회 춘추학술대회 Vol.2022 No.11

The stress triaxiality and lode angle parameters are the main factors that determine the failure criterion for ductile fracture. In this paper, a study were conducted on the relationship between the stress triaxiality ratio at failure point and the lode angle parameter for calculating the three-dimensional facture locus of API 5L X65 line-pipe steel. As a result, a non-standardized plate-type specimen was proposed.

전단응력 상태를 고려한 고강도 강재의 파괴곡선 산출

김익중(Ik-Joong Kim),장윤찬(Yun-Chan Jang),김철만(Chul-Man Kim),허용화(Yong-Hwa Heo),선종오(Jong-Oh Sun),최신범(Shin-Beom Choi),정종하(Jong-Ha Jeong) 대한기계학회 2022 대한기계학회 춘추학술대회 Vol.2022 No.11

The stress triaxiality and lode angle parameters are the main factors that determine the failure criterion for ductile fracture. In this paper, a study were conducted on the relationship between the stress triaxiality ratio at failure point and the lode angle parameter for calculating the three-dimensional facture locus of API 5L X65 line-pipe steel. As a result, a non-standardized plate-type specimen was proposed.

GTN 모델을 이용한 파괴인성 시험편의 균열진전 모사

김익중(Ik-Joong Kim),장윤찬(Yun-Chan Jang),김철만(Chul-Man Kim),김영표(Young-Pyo Kim),장윤영(Youn-Young Jang),허남수(Nam-Su Huh) 대한기계학회 2018 대한기계학회 춘추학술대회 Vol.2018 No.12

This paper presents a computational ductile fracture simulation of API 5L X70 gas pipelines using the GTN (Gurson-Tvergaard-Needleman) model. GTN model was used to analyze crack growth in SENT specimen. Experimental tests and FE analysis using the GTN model are performed for tensile bar, from which the parameters in the GTN model. Crack growth resistance curve was obtained by using SENT specimen. Consequently, comparison between FE results and experimental test result shows overall good agreements.

SENT 시험편을 이용한 실배관 파괴저항곡선 예측

김익중(Ik-Joong Kim),김영표(Young-Pyo Kim),김철만(Chul-Man Kim),장윤영(Youn-Young Jang),허남수(Nam-Su Huh),석창성(Chang-Sung Seok) 대한기계학회 2019 대한기계학회 논문집. Transactions of the KSME. C, 산업기술과 혁신 Vol.7 No.1

본 연구에서는 실 배관의 균열저항특성을 평가하기 위하여, 기존 SENB(Single edge notched bending, 단일측면노치굽힘시험)시험편 대비 실 배관과 유사한 구속조건을 갖는 것으로 알려진 SENT(Single edge notched tension, 단일측면노치인장시험)시험편을 이용하여 라인파이프 강재인 API 5L X70 의 파괴저항곡선을 산출하였다. 구속조건은 균열부위의 응력상태에 영향을 미치는 요인으로 구조물의 기하학적 형상, 균열 깊이, 하중조건, 재료물성 등에 의해 결정되는 것으로 알려져 있다. 이에, 시험편 형상 및 하중조건에 따른 구속영향을 정량적으로 평가하기 위하여, J-적분에 기초한 J-Q 해석을 수행하였다. 그리고 SENT 시험편을 이용하여 API 5L X70 강재의 파괴저항 곡선을 산출하였다. 시험은 BS 8571 규격에 따라 수행하였으며, 탄·소성 파괴역학 매개변수인 J-적분과 CTOD(Crack tip opening displacement, 균열선단개구변위)를 모두 이용하여 각각의 파괴저항곡선을 산출하였다. In this study, fracture resistance curve for API 5L X70 line-pipe steel was estimated by using SENT specimen, which is known to have similar constraint condition to the full-scale pipe. The constraints of structure are known to be affected by geometry, initial crack depth, loading condition and material properties. To assess the constraint effect for various geometries, such as SENB, SENT and full-scale pipe structure, the J-Q analysis was performed using the HRR stress field based on J-integral using finite element analysis. Fracture resistance curve obtained from SENT specimen were performed according to BS 8571 standards and J-R curve & CTOD-R curve were presented, respectively.

굽힘하중을 받는 매설 가스배관의 허용변형률 결정

김익중(Ik-Joong Kim),장윤찬(Yun-Chan Jang),유정수(Jung-Su Yoo),백종현(Jong-Hyun Baek),김철만(Chul-Man Kim),김영표(Young-Pyo Kim) 대한기계학회 2021 대한기계학회 춘추학술대회 Vol.2021 No.11

Recently, strain-based design and assessment(SBDA) method has been actively adopted to structural integrity of buried gas pipelines under ground movements. Large plastic deformation occus in pipe due to bending load, it shall be evaluated to simultaneously considering the possibility of tensile rupture and compression buckling. In this paper, various predictive formulas for calculating the allowable strain limit for gas pipelines and domestic applicability was reviewed.