PhastSystem을 이 용한 vWF Multimer의 검출

김천희 ( Chun Hee Kim ),김동제 ( Dong Jei Kim ),양성은 ( Seong Eun Yang ),박찬정 ( Chan Jeong Park ),지현숙 ( Hyun Sook Chi ) 대한임상검사과학회 1999 대한임상검사과학회지(KJCLS) Vol.31 No.2

vWF(von Willibrand Factor) is a large multimeric glycoprotein, plays a major role in mediating the adhesion of platelets during primary hemostasis. Than vWD(von Willibrand Disease) is characterized by quantitative and or qua1itative changes in vWF. Variations in the pattem of vWF multimers is important for subtyping of vWD. We have been detected the vWF multimer pattem based on the following principles. 1) SDS-agarose gel electrophoresis using PhastSystem, 2) Transfer onto a nitrocellulose membrane (diffusion transfer method), 3) Immunovisua1ization from primary and secondary antibody. From this method, we could acquire the vWF multimer band which is c1ean, efficient and reproducible.

보문 : 비이온계 계면활성제 수용액에서 면 오염포의 습윤특성과 세척성

김천희 ( Chun Hee Kim ) 韓國衣類學會 2007 한국의류학회지 Vol.31 No.3

강구조 대공간 구조시스템

김천희(Cheon Hee Kim),김춘호(Chun Ho Kim),이규복(Gyou Bok Lee) 한국강구조학회 2009 韓國鋼構造學會誌 Vol.21 No.4

유세포분석법을 이용한 말초혈액 림프구아형 (Lymphocyte Subset)검사의 정상 참고치

한상희 ( Sang Hee Han ),김천희 ( Chun Hee Kim ),조병철 ( Byung Cheol Cho ),박찬정 ( Chan Jeoung Park ) 대한임상검사과학회 2002 대한임상검사과학회지(KJCLS) Vol.34 No.2

To estimate the reference values of peripheral blood lymphocyte subsets, authors meas-ured CD3+, CD4+/CD3+, CD8+/CD3+, CD3-/CDI6+or CD56+ and CDI9+ cells in 37 hea1thy adults (29 males, 8 females, aged from 20 to 49 years) and 12 children (3 males, 9 females aged from 3 months to 7 years). EDTA blood samples were obtained from healthy subjects who had no history of viral or other illnesses or medication in Asan Medical Center during the period of January to November, 200 1. Measurement of peripheral blood lymphocytes subsets was performed by flow cytometry (FACScan, Becton-Dickinson Co., USA). The reference ranges follow; for healthy adults T cel1 (CD3\ 54-82%; T helperjinducer (Thfi cell) (CD4+/CD3\ 24-55%; T suppressor (Tsjc)cell (CD8+/ CD3\ 11-38%; B cell (CDI9\ 5-23%; NK cel1 (CD3-/1 6+ or 56\ 5-35%; and for children T cell (CD3\ 60-82%; πlji cell (CD4+/CD3+), 24-49%; Tsjc cell (CD8+/CD3\ 15-35%; B cel1 (CDI9\ 11-27%; and NK cell(CD3-jI6+or 56\ 4-20%. In healthy adults the mean percentages of T helper/inducer cells and natural killer cells showed an increasing tεndency rε, latεd to incrεasing ages, and those of T suppressor/cytotoxic cells showed the reverse results. In children the percentages of natural killer cells showed lower resu1ts and those of B cells showed higher resu1ts than in adu1ts. Our data obtained by the measurement of peripheral blood lymphocyte subsets will serve as the reference values in adults and children.

자동혈구계산기 SE-9000의 IMI channel과 HPC mode의 유용성 평가

임영근 ( Young Keun Lim ),김천희 ( Chun Hee Kim ),강윤희 ( Yun Hee Kang ),박찬정 ( Chan Jeong Park ),지현숙 ( Hyun Sook Chi ) 대한임상검사과학회 2000 대한임상검사과학회지(KJCLS) Vol.32 No.3

Background SE-9000(Sysmex, Japan) automated cell counter provides an estimate of immature cells referred to as heamopoietic progenitor cells (HPC). The purpose of this study was the evaluation of the Heamatology Analyzer, SE-9000 IMI(lmmature cell information) channel, HPC mode. Method 140 peripheral blood stem cell collections were achieved from the stem cell collection room. The linearity and precision of HPC and 1M! count and the correlation of the HPC count and CD 34+ stem cell count in peripheral blood stem cell collections. Result : The linearities of 1M! and HPC for SE-9000 was r=0.9878 and r=0.9592 each. The cefficients of vareations for 1MI and HPC of SE-9000 were : for HPC(low), 114.2%; for HPC(medium), 37.9%; for HPC(lrigh), 17.9%; for IMI(low), 56.5%; for 1MI (medium), 21.9%; for 1MI (high) , 31.4% respedtively. 1MI and HPC count showed the positive correlation with CD 34+ stem cell count with the correlation coefficients of 0.6633. Conclusion π1e SE-9000 enables the easy and rapid determination of the IMI and HPC count. the 1M! and HPC count shows good correlation with CD 34+ stema cell count by ISHAGE guideline using Flowcytometry. Whereas CD 34+ stem cell count is expensive and examined for long time. The 1M! and HPC count of SE-9000 is clinically useful for monitoring the peripheral blood stem cell count.

골수이식 후 미성숙 망상적혈구의 유용성 평가

서숙원 ( Suk Won Seo ),김천희 ( Chun Hee Kim ),지현숙 ( Hyun Sook Chi ) 대한임상검사과학회 2004 대한임상검사과학회지(KJCLS) Vol.36 No.1

Bone marrow transplantation(BMT) is widely used as curative means of various malignant and nonmalignant hematologic disorders, and early and accurate determination of engraftment is very important for critical management decisions. Reticulocyte counts performed by automated flow cytometric methods is a good indicator of erythropoietic activity and its evaluation has been proposed as an early predictor of bone marrow regeneration. Some reports highlighted the usefulness of the percentage of highly fluorescent reticulocytes and the sum of highly and medium fluorescent reticulocytes(immature reticulocyte fraction, IRF). In Asan Medical Center, the criteria for engraftment following BMT or PBSCT was defined as the first day of a 3-day trend of absolute neutrophil count(ANC)≥ 500/uL and platelet count≥ 30× 103/uL. In 1999, Grotto et al proposed an indidator of bone marrow recovery as the first day on which the IRF was twice the minimum value after bone marrow transplantation. To compare the both criterias, we got consecutive datas of immature reticulocyte fraction, absolute neutrophil count(ANC), WBC count, platelet count and reticulocyte count by XE-2100 automated hematology analyzer(Sysmex Co. Japan) from 33 patients daily after BMT. When compared to standard neutrophil engraftment(10-30 days, 16.2 ± 4.6 days), IRF engraftment (5-21 days, 11.0 ± 3.9 days) occured significantly earlier in 87.9% of patients(P<0.05). The mean engraftment day for WBC count(11-29 days, 16.4 ± 4.3 days) was similar to ANC, but platelet count and reticulocyte count revealed more delayed data (10-49 days, 19.1 ± 7.4 days vs 17-64 days, 31.4 ± 14.4 days). In conclusion, our results confirm that an increase in the immature reticulocyte population is the earliest sign of the hematopoietic recovery after BMT and that automated reticulocyte quantification including immature fraction may be integrated into clinical protocols to evaluate bone marrow reconstitution.

Factor 6 검사에 Heparin이 미치는 영향에 대한 증례 보고

김승일 ( Seung Il Kim ),최성욱 ( Sung Wuk Choi ),최미옥 ( Mi Ok Choi ),김천희 ( Chun Hee Kim ),최수진 ( Su Jin Choi ),지현숙 ( Hyun Sook Chi ) 대한임상검사과학회 2002 대한임상검사과학회지(KJCLS) Vol.34 No.2

Factor vi assay is a test for diagnosis of hemophilia A, and a way to monitor after factor replacement therapy and risk assessment of premature atherosc1erotic vascu1ar disease. The purpose of this study was to suggest the cases in which the factor vi assay was affected by heparin contamination in factor vi assay samples drawn from heparin therapy patients. According to the guide for the Factor vi assay in NCCLS (National Committee for Clinical Laboratory Standards) , two types of reference curves are employed, an extended curve and a working curve. ``The reference and patient results should be inspected for out1iers, parallelism, and linearity. We perform a factor vi assay according to the NCCLS guideline 4 point calibration curve and 1: 10, 1:20 di1uted samples for patients. However, 4 cases of heparin contamination showed the reverse resu1t of the patient``s sample. There was an exact error in linearity and parallelism between the standard curve and patients curve. The dilution used for patients of heparin contamination are 1: 10 to 1:40 때d compare with the calibration curve. We analyzed the heparin concentration on heparin contaminated plasma. If you assay three di1utions of test plasma on the patients, you could detect the error of the resu1t affected heparin.

NRBC검사에 었어서 XE-2100과 Manual N-RBC Count의 비교

장우혁 ( Woo Hyuck Chang ),김천희 ( Chun Hee Kim ),조병철 ( Byeng Chul Cho ),지현숙 ( Hyun Sook Chi ) 대한임상검사과학회 2000 대한임상검사과학회지(KJCLS) Vol.32 No.3

Background : π1e presence of NRBC is normal for frrst several days in newbom. π1e presence of NRBC in the peripheral blood in adu1t is not associated with a specific single c1inical diagnosis, but in other situations the appearance of NRBC indicates disordered erythropoiesis. In many cases, NRBC in peripheral blood is an important marker of significant hematological and non-hematological diseases. NRBC should therefore be identified precisely Most automated hematology analyzers have a flagging system to indicate the presence of NRBC in a sample qua1itatively. πus makes it necessary to correct the WBC count by substrating the number of NRBC according to manual microscopy. But, manual microscopic examination has low sensitivity, specificity, and reproducibi1ity. Method Peripheral blood specimens anticoagulatεd with EDTA wεrε studiεd. 100 samples were analysed on XE-2100 and manual NRBC count. Result Manual microscopic reference NRBC counts of 10 patient specimens showed good correlation with XE-2100 (y= 1.2692x - 0.4722 ; r = 0.9354). But, the manual microscopic NRBC result counted 100 cells indicates low correlation (y=0.7787x + 7.6241 ; r = 0.8640). Performance for with-in day precison showed a mean coefficient of variation (CV) for the XE-2100 of = 5.4% (2.0-8.4%), with a mean CV for manual NRBC counts of 22.1 %. The between day precision using QC material (SF-CHECK), was 2.94% for low control 1.29% for normal control, and 1.63% for hlgh control. Conclusion : We conc1ude that thls automated method using XE-2100 was suitable for accurate NRBC counting in peripheral blood specimens with improved performance in terms of accuracy, reproducibi1ity, and sensitivity when compared to manual microscopic methods.

XE-2100을 이용한 망상적혈구수와 미성숙망상적혈구 분획의 측정

최미옥 ( Mi Ok Choi ),김천희 ( Chun Hee Kim ),조병철 ( Byung Chul Cho ),지현숙 ( Hyun Sook Chi ) 대한임상검사과학회 2000 대한임상검사과학회지(KJCLS) Vol.32 No.3

We evaluated reticulocyte counting and measurement of inlmature reticulocyte fraction (IRF) with XE-21oo automated CBC analyzer (Sysmex Co. Japan). Reticulocyte counts and inlmature reticulocyte fraction were compared with those obtained by the R-3000 automated reticulocyte analyzer (Sysmex Co. Japan). To establish reticulocyte and immature reticulocyte fraction reference values, we measured reticulocyte count and immature reticulocyte fraction in healthy adult population. We performed with-in day precision studies on 3 patients showing low, medium, and high percentage of reticulocyte and between-day precision studies with Q.C material (Re-check Sysmex Co. Japan). The result were summarized as fol1ows 1. Comparison of XE-21oo and R-3000 : There was some variation of correlation between the level of reticulocyte counts such as r=0.75 in less than 0.5% group, r=0.75 in 0.51-1.80% group and r=0.78 in more than 1.81% group. 2. Reference value of reticulocyte count : Adult 0.6% - 2.0%, Reference value of inlmature reticulocyte fraction : Adult 1. 1 % - 11.3 % 3. Precision : With-in day precision (n=9) : The C.V of the reticulocyte counts for low (meanO.80%), medium (mean2.l5%), and high (meanI4.69%) reticulocyte count specimens was 7.27%, 4.08%, and 3.64%, respectively. Between day precision (n=23) : The C.V of the reticu10cyte and immature reticu10cyte fraction were 2.84% and 12.6%, respectively.

D-dimer 측정의 ELISA법과 STA LIAtest D-DI법의 비교

권수진 ( Su Jin Kwon ),김천희 ( Chun Hee Kim ) 대한임상병리사협회 1999 임상혈액검사학회 발표자료집 Vol.5 No.1

Background : The specific degradation of fibrin is reactive the mechanism responding to the formation of fibrin. Plasmin is the fibrinolytic enzyme derived from the inactive plasminogen. Plasmin breaks down fibrin. The D-dimer is the terminal fragment in the degradation of fibrin. To evaluate D-dimer as a marker for firinolysis in various kinds of thrombotic disorders, the quantitative study is increasing tendency for rapid diagnosis and monitoring of therapy. To get the rapid quantitative test results, more convenient and automated determination method is necessary. Thus Liatest D-Di, a new fast assay to determine D-dimers, has performances comparable to classical ELISA method. Method: This study was performed on 81 plasma samples. D-dimer measured. D-dimer was measured with STA-Liatest D-Di, an automated instrument using latex beads coated with 2 monoclonal D-dimer antibodies and agglutinated in presence of D-dimer. Results were compared with those obtained with the reference ELISA method.(Asserachrom D-dimer) Result: Among the 81 patients, 23 resented D-Di (STA-Liatest)>1000ng/ml (normal values<400 ng/ml). Thirty-five patients out of 81 had D-Di (STA-Liatest)<400 ng/ml and 46 patients presented high levels of D-Di(STA-Liatest)>400 ng/ml. The STA-Liatest D-dimer resluts were well correlated with it resluts obtained with reference method (Asserachrom D- Di, Coefficient of Correlationp r=0.76. p<0.01). Conclusion : Automated immunoturbidimetric STA-Liatest D-dimer was a rapid quantitative D-dimer assay and well adapted for emergency thats and : results were well correlated with those resluts obtained from the ELISA method.