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원저(原著) : 활성화 C 단백 내성과 혈액응고 V 인자 돌연변이
박숙자 ( Sook Ja Park ),김동제 ( Dong Jei Kim ) 대한임상병리사협회 1996 임상혈액검사학회 발표자료집 Vol.3 No.1
Only 5~10 % of the inherited thrombophilia is thought to be associated with deficiencies of protein C, protein S or antithrombin III. Approximately half of the inherited thrombophilia has been found to have resistance to-activated protein C(APC). The APC resistance phenotype is associated with a single point mutation in the factor V gene that changes Arginine(506) in the APC cleavage site to a Glutamin. We performed quantitative test for protein C, protein S and free protein S in 42 thrombophilic patients. Then we did functional clotting time test for APC resistance and polymerase chain reaction for the detection of factor V gene mutation assay. In 42 patients, 29 patients had protein C or protein S deficiencies. Most of the patients with protein C or protein S deficiencies had thrombotic episodes as an initial presentation(37 %). However there were two thrombosis patients in normal distribution of protein C and protein S. Three patients showed significant resistance to APC. These patients also had mild protein C or protein S deficiencies. The factor V mutation didn`t find in 42 patients of this study. Further study for dectection of factor VIII mutation or other type of factor V mutation is necessary to find out more cases of inherited thrombophilia.
권수진 ( Su Jin Kwon ),김동제 ( Dong Jei Kim ),김덕희 ( Duk Hee Kim ) 대한임상병리사협회 1997 임상혈액검사학회 발표자료집 Vol.4 No.1
Neutrophils are the rapid deployment and effector arm of the immune system. They are present in large numbers in the circulation, through which they rapidly transit en route to tissiue, where they form the first line of celluar defense against invading microorganism. To fufill this defensive role, circulating neutrophils must adhere to the endothelium of capillaries and venules adjacent to the inflammatory locus, migrate through the vessel wall to the area of inflammation, phagocytose opsonized bacteria, kill ingested organisms and, finally, inactivate their own toxic products to prevent damage to normal tissue. Different aspects of this complex process, such as chemotaxis and phagocytosis, can be separately defined and studied in vitro but they are clearly linked by common mechanism. Margination, chemotaxis and phagocytosis all depend upon neutrophil adhesion. Respiratory burst activity depend upon generating reactive oxygen species with potent microbicidal activity. The complexity of the neutrophil response to inflammatory creates many difficulties for the study of neutrophil function in vitro. Subsequent function tests must be selected with an understanding of any associated technical problems. The investigation of abnormal neutrophil responses may necessitate the screening of several tests of function.
PhastSystem을 이 용한 vWF Multimer의 검출
김천희 ( Chun Hee Kim ),김동제 ( Dong Jei Kim ),양성은 ( Seong Eun Yang ),박찬정 ( Chan Jeong Park ),지현숙 ( Hyun Sook Chi ) 대한임상검사과학회 1999 대한임상검사과학회지(KJCLS) Vol.31 No.2
vWF(von Willibrand Factor) is a large multimeric glycoprotein, plays a major role in mediating the adhesion of platelets during primary hemostasis. Than vWD(von Willibrand Disease) is characterized by quantitative and or qua1itative changes in vWF. Variations in the pattem of vWF multimers is important for subtyping of vWD. We have been detected the vWF multimer pattem based on the following principles. 1) SDS-agarose gel electrophoresis using PhastSystem, 2) Transfer onto a nitrocellulose membrane (diffusion transfer method), 3) Immunovisua1ization from primary and secondary antibody. From this method, we could acquire the vWF multimer band which is c1ean, efficient and reproducible.
이창수 ( Chang Soo Lee ),박찬정 ( Chan Jeoung Park ),강소영 ( So Young Kang ),김지명 ( Ji Myung Kim ),김동제 ( Dong Jei Kim ),조병철 ( Byung Cheol Cho ),지현숙 ( Hyun Sook Chi ) 대한임상검사과학회 2002 대한임상검사과학회지(KJCLS) Vol.34 No.2
The CA-7000™(Sysmex Co., Japan) is a completely automated coagulation analyzer that performs clotting, chromogenic and immunological tests. We evaluated the analytical performances and usefulness of this analyzer. Neoplastin Cl Plus(Diagnostica STAGO, France), ThromborelR S(lSI 1.01, Dade Behring, USA), P1T A 5(Diagnostica STAGO, France) and Actin FS(Dade Behring, USA), CaCh(Dade Behring, USA) were used for measurement of prothrombin time(PT) and activated partial thromboplastin time(aP1T). We evaluated the precision and accuracy of PT and aP1T. Comparision study was performed by comparing the results of STA(Diagnostica STAGO, France) with those of CA-7000™. Interference of hemoglobin, lipid and bilirubin in the PT and aPTT measurement was examined. The test results measured by commercial plasma(Coag-N and Coag-P, Diagnostica) with designated values showed satisfactory accuracy. The coefficients of variation of both within-run and between day precision for CA-7ωo ™ were below 2 %. The comparison study indicated the a good correlation between resu1ts of ST A and thosε of CA-7α)()TM. Various concentrations of hemoglobin, lipid and bilirubin made no influence on PT TκI and aP1T results. We concluded that the CA-7α)()UV1 offered excel1ent perfonnances and provided high throughput for stat coagulation tests. The CA-7000TUh‘ V1 is expected to contribute to the highest efficiency in clinical laboratories.