장시간의 일회성 지구성 운동 수행 시 노화에 의한 쥐 골격근의 NF-ĸB 신호전달 변화

김형준(Kim Hyung-Jun),권형태(Kwon Hyeong-Tae),손희정(Son Hee-jung),채정훈(Chai Jung-Hoon),어수주(Eo Su-Ju),김창근(Kim Chang-Keun),김효정(Kim Hyo-Jung) 한국체육과학회 2011 한국체육과학회지 Vol.20 No.3

Nuclear factor kappa B (NF- kB) is an important transcription factor of skeletal muscle atrophy. Several studies have been explored the possibile pathways which may be modulated with exercise and age. The purpose of this study was to examine the effect of one single bout of endurance exercise on nuclear factor kappa B (NF-kB) activation and MuRF1 expression in old rat. 32 rats were used and were divided into two groups by age; 20 weeks (n=16) vs 72 weeks (n=16). Each experimental group was further divided into two subgroups; Before exercise (n=8) and after exercise (n=8). All animals in exercise groups performed a 3 hr swimming exercise (30min x 6 bouts with 5min rest). Following the swimming exercise, the protein level of NF-kB (p65) subunit was increased in both old and young group(p<.05). The protein level of NF-kB (p65) was higher in old than young group before exercise (p<.05) and the expression of MuRF1 in only old group was increased with the swimming exercise(p<.05). On the other hand, In this study phospho-IkBa was unchnaged (p>.05). These results suggest that the expression of nuclear factor kappa B (NF- kB) with endurance exercise may a key role in muscle atrophy in aged group.

암흰쥐에서 합성 Gonadotrophin releasing hormone 의 투여가 혈청 Luteinizing hormone 의 수준 및 자궁중량에 미치는 영향

정영채,김창근,김형태,정영호,양광식 ( Yung Chai Chung,Chang Keun Kim,Hyung Tae Kim,Yung Ho Chung,Kwang Sik Yang ) 한국축산학회 1982 한국축산학회지 Vol.24 No.2

The purpose of this experiment was to investigate the effects of synthetic gonadotropin releasing hormone (GnRH) administration on serum LH level and uterine weight in mature female rats. The rats were given a intraperitoneal injection of synthetic GnRH at 1:00 PM of proestrus, and were sacrificed immediately after administration, at 2:00, 3:00, 4:00, 5:00, 6:00, 7:00, 8:00 PM to investigate the changes of serum LH level and uterine weight. 1. In control group, the serum LH level increased from 4.35±0.84 mIU/㎖ at 1:00 PM to a preovulatory LH surge about 11.05±1.4 mIu/㎖ at 5:00 PM and then decreased gradually. The serum LH level in group treated with synthetic GnRH remarkably increased from 4.20±0.30 mIU/㎖ at 1:00 PM to a peak level, 13.17±0.17 mIU/㎖ at 2:00 PM, 1 hour after administration. The time required up to the preovulatory LH surge in treated group was shortened about 3 hours as compared to that of control group. 2. The weights of uterus in group treated with synthetic GnRH were slightly heavier than those of control group at 1, 2 and 3 hour after administration, but there were no significant differences between the control and treated group.

가임신 흰쥐에 있어서 자궁척출 및 Prostaglandin F2α 투여가 혈청 Progesterone 의 농도 및 황체에 미치는 영향

김형태,정영채,김창근 ( Hyung Tae Kim,Yung Chai Chung,Chang Keun Kim ) 한국축산학회 1981 한국축산학회지 Vol.23 No.4

The purpose of this experiment was to investigate the effects of hysterectomy and administration of PGF₂α on serum progesterone level and corpus luteum in pesudopregnant rats. PGF₂α administration or hysterectomy was performed at day S of pseudopregnancy. Five rats from each group were randomly sacrificed at day 5,6,7,8,9 and 12 of pseudopregnancy for assay of serum progesterone level and microscopic observation of ovary. The results obtained were as follows; 1. In both normal and hysterectomized groups, PGF₂α administration caused significant reduction in serum progesterone level to 24.2% and 30.8% at day 6 and to 11.3% and 34.3% at day 9 of pseudopregnancy as compared with pretreatment level. The serum progesterone level of hysterectomized group untreated with PGF₂α remained elevated until day 12 of pseudopregnancy as compared to those of other three groups. 2. The ovarian weights of control group treated with PGF₂α were significantly reduced as compared to those of other three groups at day 9 of pseudopregnancy (p$lt;0.05). 3. The number of corpus luteum in groups treated with PGF₂α was slightly reduced, but there were no significant difference between PGF₂α and non-PGF₂α treated groups at day 6,7,8,9 of pseudopregnancy. 4. The number of lutein cell in corpus luteum of PGF₂α treated groups on day 9 of pseudopregnancy was significantly lower than those of PGF₂α untreated groups (p$lt;0.05).

유색미 겨 아라비녹실레인과 운동트레이닝이 Lipopolysaccharide 처치된 흰쥐의 면역인자 및 염증억제에 미치는 영향

손희정 ( Hee Jeong Son ),김형준 ( Hyung Jun Kim ),채정훈 ( Jeong Hoon Chae ),권형태 ( Hyung Tae Kwon ),여효성 ( Hyo Seong Yeo ),어수주 ( Su Ju Eo ),임예현 ( Yea Hyun Leem ),김효정 ( Hyo Jeong Kim ),김창근 ( Chang Keun Kim ) 한국응용생명화학회 2012 Journal of Applied Biological Chemistry (J. Appl. Vol.55 No.1

Arabinoxylan, Ara, rice bran has been shown to be a potent biological response modifier as manifested by stimulation of different arms of the immune system. We examined the effects of Ara rice bran and exercise on the immune function and cytokines in lipopolysaccharide (LPS)-stimulated rats. As the results, tumor necrosis factor-α as representative inflammatory cytokines showed a significantly lower in Ara supplement group, thus the Ara rice bran had a higher inhibitory activity than the both exercise and control group. However, 4 weeks of exercise training significantly increased inflammatory reactions rather than treatment with Ara in LPS-treated rats. The Ara rice bran acted to decrease the inflammatory reaction. These results suggest that the supplement of Ara rice bran is likely contribute to inflammation response and the Ara rice bran can be used as a possible safe alternative to the immunotherapeutic modalities.

수태 전과 수태 중 모체운동 훈련이 어미 쥐와 새끼 쥐의 골격근 세포 내 미토콘드리아 생합성 요소에 미치는 영향

전유나 ( Yu Nah Jeon ),권형태 ( Hyeong Tae Kwon ),김형준 ( Hyung Jun Kim ),여효성 ( Hyo Seong Yeo ),김효정 ( Hyo Jeong Kim ),김창근 ( Chang Keun Kim ) 한국운동생리학회(구 한국운동과학회) 2014 운동과학 Vol.23 No.2

이 연구는 수태 전 혹은 수태 중 모체의 유산소 운동 훈련이 자식의 근 세포 내 미토콘드리아 생합성 요소에 미치는 영향을 평가하려는 목적으로 계획되었다. 10주령 Sprague-Dawley 암컷쥐(Samtako, Korea)를 대조(CON), 수태 전 운동(EBP), 수태 중 운동(EDP) 그룹으로 구분하여 각각 7마리씩 총 21마리를 사용하였다. 운동그룹은 총 10주(EBP) 및 3주(EDP)간 주 5회, 일일 1시간, 동물용 트레드밀에서 60-75 %V O2max 강도로 운동훈련을 실시하였다. 수태 전?중 모체의 운동훈련 효과와 태어난 새끼 쥐의 미토콘드리아 생합성 요소를 시기별(출산 직후, 출산 8주후)로 평가하기 위해 미토콘드리아 생합성(COX-Ⅳ, mtTFA, PGC-1α)과 모세혈관형성(VEGF165, VEGFtotal, HIF-1α) 인자들의 발현을 western blot 기법으로 분석하였다. 실험결과 수태 전 운동수행 그룹(EBP)과 수태 중 운동수행 그룹(EDP)에서 모체의 mtTFA(15%, 19%), PGC-1α(28%, 33%), VEGF165(14%, 19%) 단백질 발현이 대조그룹(CON)에 비해 높게 나타났다. Off-EDP 그룹의 골격근에서 출산 직후 COX-Ⅳ(35%), mtTFA(24%), PGC-1α(25%), VEGF165(17%), VEGFtotal(25%) 단백질 발현이 Off-CON 그룹에 비해 높게 나타났으며 그 영향은 8주 후까지 지속되었다. 이상의 결과를 요약하면, 수태 중인 모체의 규칙적인 유산소 훈련이 모체뿐 만 아니라 새끼 쥐의 미토콘드리아 산화 능력과 혈관 생성을 향상시키며, 새끼 쥐의 유산소 대사능력은 모체가 수태 전에 운동 한 경우보다 수태 중에 운동하는 것이 출산 후 새끼의 대사능력에 보다 더 긍정적인 영향을 미친다. The present study was to investigate whether mitochondrial biosynthetic markers of offspring is affected by maternal exercise training during pregnancy. Twenty-one SD rats (10 wks) were randomly assigned into CON (control, n=7), EBP (exercise before pregnancy, n=7) and EDP (exercise during pregnancy, n=7) group. Exercise groups ran on the rodent treadmill at 60-75 %V O2max for 10 weeks (1 h/d, 5 d/wk). Muscle samples were obtained from off springs at immediately after and 8 weeks after birth, and mother rats were also sacrificed 4 weeks after delivering the offspring. Mitochondrial biosynthetic markers, COX-Ⅳ, m tTFA, PGC-1α proteins and angiogenesis f actors, VEGF165, VEGFtotal, HIF-1α proteins were measured by western blotting. The expression of maternal PGC-1α, mtTFA, and COX-Ⅳ proteins in both EBP and EDP groups were significantly upregulated than CON group after aerobic exercise training. The expression of maternal VEGF165, VEGFtotal were significantly upregulated than CON group after aerobic exercise training, whereas no difference in HIF-1α protein level were found among the groups. The expression of COX-Ⅳ, mtTFA, PGC-1α protein levels of off springs in EDP group were similar to maternal values and significantly higher than EBP and CON groups immediately after birth and maintained up to 8 weeks after birth. The trend of angiogenesis factors, VEGF165, VEGFtotal, HIF-1α proteins of offsprings also similar to mitochondrial biosynthetic markers immediately after and 8 weeks after birth as well. In summary, prolonged and regular maternal aerobic exercise during pregnancy increases mitochondrial biosynthetic and angiogenetic factors in skeletal muscles in both maternal and offsprings. Furthermore, prolonged and regular maternal aerobic exercise during pregnancy maintains upregulated levels of mitochondrial biosynthetic and angiogenetic factors during growth and development of offsprings.