수태 전과 수태 중 모체운동 훈련이 어미 쥐와 새끼 쥐의 골격근 세포 내 미토콘드리아 생합성 요소에 미치는 영향

전유나 ( Yu Nah Jeon ),권형태 ( Hyeong Tae Kwon ),김형준 ( Hyung Jun Kim ),여효성 ( Hyo Seong Yeo ),김효정 ( Hyo Jeong Kim ),김창근 ( Chang Keun Kim ) 한국운동생리학회(구 한국운동과학회) 2014 운동과학 Vol.23 No.2

이 연구는 수태 전 혹은 수태 중 모체의 유산소 운동 훈련이 자식의 근 세포 내 미토콘드리아 생합성 요소에 미치는 영향을 평가하려는 목적으로 계획되었다. 10주령 Sprague-Dawley 암컷쥐(Samtako, Korea)를 대조(CON), 수태 전 운동(EBP), 수태 중 운동(EDP) 그룹으로 구분하여 각각 7마리씩 총 21마리를 사용하였다. 운동그룹은 총 10주(EBP) 및 3주(EDP)간 주 5회, 일일 1시간, 동물용 트레드밀에서 60-75 %V O2max 강도로 운동훈련을 실시하였다. 수태 전?중 모체의 운동훈련 효과와 태어난 새끼 쥐의 미토콘드리아 생합성 요소를 시기별(출산 직후, 출산 8주후)로 평가하기 위해 미토콘드리아 생합성(COX-Ⅳ, mtTFA, PGC-1α)과 모세혈관형성(VEGF165, VEGFtotal, HIF-1α) 인자들의 발현을 western blot 기법으로 분석하였다. 실험결과 수태 전 운동수행 그룹(EBP)과 수태 중 운동수행 그룹(EDP)에서 모체의 mtTFA(15%, 19%), PGC-1α(28%, 33%), VEGF165(14%, 19%) 단백질 발현이 대조그룹(CON)에 비해 높게 나타났다. Off-EDP 그룹의 골격근에서 출산 직후 COX-Ⅳ(35%), mtTFA(24%), PGC-1α(25%), VEGF165(17%), VEGFtotal(25%) 단백질 발현이 Off-CON 그룹에 비해 높게 나타났으며 그 영향은 8주 후까지 지속되었다. 이상의 결과를 요약하면, 수태 중인 모체의 규칙적인 유산소 훈련이 모체뿐 만 아니라 새끼 쥐의 미토콘드리아 산화 능력과 혈관 생성을 향상시키며, 새끼 쥐의 유산소 대사능력은 모체가 수태 전에 운동 한 경우보다 수태 중에 운동하는 것이 출산 후 새끼의 대사능력에 보다 더 긍정적인 영향을 미친다. The present study was to investigate whether mitochondrial biosynthetic markers of offspring is affected by maternal exercise training during pregnancy. Twenty-one SD rats (10 wks) were randomly assigned into CON (control, n=7), EBP (exercise before pregnancy, n=7) and EDP (exercise during pregnancy, n=7) group. Exercise groups ran on the rodent treadmill at 60-75 %V O2max for 10 weeks (1 h/d, 5 d/wk). Muscle samples were obtained from off springs at immediately after and 8 weeks after birth, and mother rats were also sacrificed 4 weeks after delivering the offspring. Mitochondrial biosynthetic markers, COX-Ⅳ, m tTFA, PGC-1α proteins and angiogenesis f actors, VEGF165, VEGFtotal, HIF-1α proteins were measured by western blotting. The expression of maternal PGC-1α, mtTFA, and COX-Ⅳ proteins in both EBP and EDP groups were significantly upregulated than CON group after aerobic exercise training. The expression of maternal VEGF165, VEGFtotal were significantly upregulated than CON group after aerobic exercise training, whereas no difference in HIF-1α protein level were found among the groups. The expression of COX-Ⅳ, mtTFA, PGC-1α protein levels of off springs in EDP group were similar to maternal values and significantly higher than EBP and CON groups immediately after birth and maintained up to 8 weeks after birth. The trend of angiogenesis factors, VEGF165, VEGFtotal, HIF-1α proteins of offsprings also similar to mitochondrial biosynthetic markers immediately after and 8 weeks after birth as well. In summary, prolonged and regular maternal aerobic exercise during pregnancy increases mitochondrial biosynthetic and angiogenetic factors in skeletal muscles in both maternal and offsprings. Furthermore, prolonged and regular maternal aerobic exercise during pregnancy maintains upregulated levels of mitochondrial biosynthetic and angiogenetic factors during growth and development of offsprings.

다이버의 일회성 저수온 노출이 혈중 FGF21, Irisin 및 T3에 미치는 영향

전유나 ( Yu Nah Jeon ),라승구 ( Seung Goo Ra ),김창근 ( Chang Keun Kim ) 한국운동생리학회 2015 운동과학 Vol.24 No.3

PURPOSE: It has been understood that brown fat or beige adipose tissues can facilitate heat production through non-shivering thermogenesis. FGF21, irisin, or T3 hormones are believed to enhance brown fat activation and increase in cold environment and exercise. The purpose of this study was to determine the changes of circulating FGF21, irisin, T3 hormone concentrations during the acute diving activity in divers who have been undergone long-term intermittent cold exposure. METHODS: Seven SCUBA divers who have been diving at least for 5 years with 50 to 100 diving records per year participated in this study. A bout of acute diving was performed in the east sea (13-14 ℃) for 30 min. Blood samples were obtained from arm vein at pre- and post-diving, and after an hour recovery. Serum FGF21, Plasma irisin and serum T3, TG and FFA were assayed. RESULTS: Serum FGF21 level was increased after 30 min of diving (p=.043, 138%) compared to pre-diving and after one hour of recovery to pre-diving level (p=.018, 65%). However, plasma irisin, T3 , FFA, TG levels were unchanged both after post-diving and one hour recovery (p>.05). CONCLUSIONS: It is concluded that heat generation through non-shivering thermogenesis may play a major role during the acute diving activity in divers who have been undergone long-term intermittent cold exposure.

복합재활운동 프로그램이 슬관절 전치환술 노인 환자의 근력, 이동능력 및 균형감각에 미치는 영향

여효성(Yeo, Hyo-Seong),전유나(Jeon, Yu-Nah),하재여(Ha, Jae-Yeo) 한국웰니스학회 2017 한국웰니스학회지 Vol.12 No.3

이 연구는 퇴행성 관절염 환자의 슬관절 전치환술 후 저하되는 신체능력을 회복시킬 수 있는 효과적인 재활방법을 구명하고자 계획되었다 따라서 슬관절 전치환술 후 초기에 노인 여성 환자에게 복합재활운동 . 프로그램을 적용하여 하지의 근력, 이동능력, 균형감각의 변화를 확인하고자 하였다. 연구의 목적을 위해, 퇴행성 관절염을 가진 65세 이상 노인 여성 14명을 복합재활운동 집단(n=7)과 가정운동집단(n=7)으로 구분하였다. 복합재활운동 집단은 12주 동안 저항성 운동과 균형 운동을 병행하여 실시하였으며, 가정운동집단은 낮은 강도의 전통적인 재활운동을 실시하였다. 수술 전과 수술 후 12주간의 재활이 종료된 시점에 하지의 최대자발적 근 수축력, 이동 능력 그리고 균형능력을 측정하였다. 12주간의 복합재활운동을 실시한 노인 여성의 근력은 수술 전과 유의한 차이를 보이지 않았으나(p=.275), 가정운동을 실시한 집단의 근력은 수술 전에 비해 유의하게 낮았다(p=.001, -35.2%). 이와 유사하게 복합재활운동을 실시한 노인 여성의 이동능력은 수술 전과 유의한 차이를 보이지 않았지만(p=.639), 가정운동 집단에서는 수술 전에 비해 유의하게 낮았다(p=.002, -13.7%). 한편, 가정운동 집단의 균형능력은 수술 전 · 후 차이가 없었으며(p=.647), 복합재활운동집단의 균형능력은 훈련 후 수술 전에 비해 향상된 것으로 나타났다(p=.005, 12.5%). 이러한 결과들을 통해 복합재활 운동이 노인 여성 슬관절 전치환술 환자들의 신체기능 회복 및 기능개선에 긍정적인 영향을 미칠 가능성이 제안된다. The purpose of this study was to investigate the effective rehabilitation to regain physical abilities reduced after total knee arthroplasty (THA) of degenerative arthritis patients. Therefore, we applied combined rehabilitation exercise to aged female patients at early period after THA and observed the changes of lower limb muscle strength, mobility and balance. To purpose of the study, fourteen elderly female patients (age > 65yrs) undergone degenerative arthritis were assigned into combined rehabilitation exercise group (n=7) and home exercise group (n=7). Combined rehabilitation exercise group performed resistance exercise and balance exercise together and home exercise group conducted low level of traditional rehabilitation at home for 12 weeks. Maximal voluntary muscle contraction, mobility, and balance were measured before the surgery and after the 12 wk of rehabilitation. Muscle strength of combined rehabilitation exercise group was not changed (p=.275), however home exercise group showed significant lower muscle strength (p=.001, -35.2%). Likewise, in case of mobility, it was not changed in combined rehabilitation exercise group (p=.639), but reduced in home exercise group (p=.002, -13.7%). On the other hand, balance ability was not changed in home exercise group (p=.647), but significantly increased in combined exercise group after the training (p=.005, 12.5%). These results suggest that combined rehabilitation exercise may have a positive effect on recovery of physical function and ability of elderly female patients undergone THA.

규칙적인 걷기 운동이 과체중 남성 노인의 혈중 Irisin 및 FGF-21 농도에 미치는 영향

박수정(Park, Su-Jung),전유나(Jeon, Yu-Nah),김창근(Kim, Chang-Keun) 한국체육과학회 2017 한국체육과학회지 Vol.26 No.5

This study was performed to investigate the effect of regular walking exercise on plasma irisin and FGF-21 concentration, obesity related variables, and body composition in overweight old men. Ten older men (> 65 yrs) were divided into two groups, CON (control, BMI 18-25 kg/㎡) and OWT (overweight, BM I> 25kg/㎡) group. Both groups performed walking exercise at the intensity of 70% HRmax for 12 weeks. Target heart rate during walking exercise was monitored using polar heart checker. Plasma irisin and FGF-21 concentration, total cholesterol, LDL-C, HDL-C, glucose, and insulin were analyzed before and after the exercise training. Regular walking exercise significantly increases in plasma irisin concentration in both CON(p=.016) and OWT(p=.038) groups. However, serum FGF-21 concentration was significantly lower in OWT group than that of CON group before(p=.017) and after(p=.001) the walking training, but reamin unchanged after the walking training in both groups(p<.05). In addition, blood glucose level was significantly reduced in both groups(p=.041, p=.013) after walking exercise training. HDL-C concentration has markedly increased in both groups(p=.015, p=.049) after walking exercise training. Therefore, we suggest that regular walking exercise training may has an important role in white fat browning by the increased irisin concentration, and have a positive effect on glucose and lipid metabolism in both normal and overweight older men.