적색색소를 생산하는 해양미생물 JE-34 균주의 분류학적 특성 및 항균활성

김주상,김만철,한용재,허문수,Kim, Ju-Sang,Kim, Man-Chul,Harikrishnan, Ramasamy,Han, Yong-Jae,Heo, Moon-Soo 한국미생물학회 2009 미생물학회지 Vol.45 No.4

A red pigment-producing bacterial strain was isolated from sediment sample of the East China Sea. The isolate was identified by analysis based on 16S rDNA sequence and morphological, physiological properties, biochemical characteristics and fatty acid composition. Phylogenetic analysis based on 16S rDNA sequence showed that isolate represent a phyletic lineage within the genus Zooshikella, and this strain was most closely related to Zooshikella ganghwensis KCTC $12044^T$ (AY130994) (99.79%). The strain was Gram-negative, aerobic and required NaCl at 0.5~8.0% for growth. The predominant cellular fatty acids were saturated and monounsaturated straight-chain fatty acids. Consequently, this strain was identified as a member of the genus Zooshikella and designated as Zooshikella sp. JE-34. The pigment showed characteristics similar to prodigiosin, a well-known red pigment previously detected in Serratia marcescens. The antimicrobial activity of Zooshikella sp. JE-34 bacterial pigment was tested against 18 microorganisms, which were fish and human pathogens. The Zooshikella sp. JE-34 red pigment showed high antimicrobial activity against Streptococcus iniae, S. parauberis, S. mutans, Staphylococcus aureus, and Propionibacterium acnes. 동중국해 해양퇴적층에서 다량의 적색색소를 생산하는 해양미생물을 분리하였다. 분리균주의 분류학적 특성의 확인을 위해 형태학적, 생리학적, 생화학적 특성, 세포지방산 분석, 16S rDNA 염기서열을 이용한 분자 계통학적 분석을 통해 분류학적 위치를 탐색하였다. 분자 계통학적 분석 결과 Zooshikella 속에 포함되는 것으로 확인되었고 Zooshikella ganghwensis KCTC $12044^T$ (AY130994)와 99.79%의 가장 높은 유사도를 보였다. 분리균주는 그람음성의 간균으로 호기성 및 NaCl 의존적인 배양특성을 보였다. 세포 지방산 분석결과 type strain과 주요 지방산 조성이 유사하였으며 이러한 특성을 종합하여 Zooshikella sp. JE-34로 동정하였다. JE-34가 생산하는 적색색소는 이미 보고되어 널리 알려진 Serratia marcescens에서 생산되는 적색색소인 prodigiosin의 특성과 유사하였다. 색소물질의 인체병원균 및 어류질병 유발세균에 대한 항균활성을 검토한 결과 18종의 피검균 중 특히 Streptococcus iniae, S. parauberis, S. mutans, Staphylococcus aureus, Propionibacterium acnes에 대한 항균활성이 우수하였다.

해양방선균 Streptomyces sp. ACT-1의 동정 및 항산화 활성

김만철,김주상,김윤범,한용재,허문수,Kim, Man-Chul,Kim, Ju-Sang,Kim, Yun-Beom,Harikrishnan, Ramasamy,Han, Yong-Jae,Heo, Moon-Soo 한국미생물학회 2009 미생물학회지 Vol.45 No.4

For the research of the natural antioxidant from marine sources, an antioxidant-producing marine actinomycetes was isolated from sea water in Jeju coastal area. The strain was identified based on 16S rDNA sequencing, the morphology by a method of scanning electron microscopy, physiological and biochemical characteristics and cellular fatty acid analysis. The isolated strain ACT-1 cell size was $0.5\sim1.0{\mu}m$ and gram positive, aerobic, nonmotile, substrate mycelium are red and gray aerial mycelium. 16S rRNA sequence analysis showed that were Gram-positive bacteria grouped on Streptomyces genus. Results of cellular fatty acid analysis showed that major cellular fatty acids were $C_{15:0}$ anteiso (39.33%), $C_{16:1}$ cis 9 (11.96%), $C_{16:0}$ (13.08%) and $C_{17:0}$ anteiso (10.99%). Finally, strain was identified Streptomyces sp. ACT-1. The antioxidant activity of methanol extract from Streptomyces sp. ACT-1 was evaluated by measuring DPPH, hydroxyl, and alkyl radical scavenging activity using an electron spin resonance (ESR) spectrometer. DPPH radical scavenging activity of SBME-1 (Streptomyces broth methanol extract) was 67% at $1,000{\mu}g$/ml. Hydroxyl radical scavenging activity of SBME-1 was 84% at $500{\mu}g$/ml. Alkyl radical scavenging activity of SBME-1 was 71% at $1,000{\mu}g$/ml. 이 연구는 해양 유래의 천연 항산화물질에 관한 연구로서 항산화물질 생산 해양 방선균은 제주연안 해수에서 분리되었다. 균주는 16S rRNA 염기서열, 전자현미경에 의한 형태학적, 생리, 생화학적 및 세포 지방산 분석을 기초로 동정되었다. 분리균주 ACT-1은 그람양성, 호기성, 비운동성, 기질균사체색이 red, 기중균사체 색이 gray계열 이었다. 세포지방산 분석결과 주요 지방산으로 $C_{15:0}$ anteiso (39.33%), $C_{16:1}$ cis 9 (11.96%), $C_{16:0}$ (13.08%)와 $C_{17:0}$ anteiso (10.99%)로 분석되었으며, 최종적으로 Streptomyces sp. ACT-1으로 동정되었다. Streptomyces sp. ACT-1 메탄올 추출물의 항산화활성은 DPPH (1,1-diphenyl-2-picrylhydrazyl), hydroxyl, alkyl radical 소거활성을 ESR (electron spin resonance) spectrometer를 이용하여 측정되었다. SBME-1 (Streptomyces broth methanol extract)의 DPPH 라디컬 소거활성은 $1,000{\mu}g$/ml 농도에서 67%, Hydroxyl radical 소거활성은 $500{\mu}g$/ml에서 84%, Alkyl radical 소거활성은 $1,000{\mu}g$/ml 농도에서 71%를 보였다.

백일해에 대한 연령별 면역혈청학적 연구

이수영,최의윤,김주상,안중현,최정현,마상혁,박준수,김황민,강진한,Lee, Soo Young,Choi, Ui Yoon,Kim, Ju Sang,Ahn, Joong Hyun,Choi, Jung Hyun,Ma, Sang Hyuk,Park, Joon Soo,Kim, Hwang Min,Kang, Jin Han 대한소아감염학회 2012 Pediatric Infection and Vaccine Vol.19 No.2

목적: 우리나라의 백일해에 대한 연령별 방어면역 상태를 평가하고자 면역혈청학적 연구를 시행하였다. 방법: 국내 4개 대학병원에 방문한 건강한 소아, 청소년 및 성인을 대상으로 하였다. 대상을 연령에 따라 7개 연령군으로 구분하였고 ELISA 방법을 이용하여 백일해의 항체가를 측정하였다. 연령군별 기하 평균항체가와 각 연령군별 양성 항체가를 보이는 피험자의 비율을 조사하였다. 제조사의 지침대로, 항체가가 24.0 EU/mL 이상인 경우를 양성으로 판정하였다. 결과: 전체 1,605명의 피험자가 연구에 참여하였고, 기하 평균항체가는 $56.16{\pm}50.54EU/mL$였다. 연령군별로는 11세 미만 소아 연령군($64.78{\pm}53.24EU/mL$)이 가장 높은 기하 평균항체가를 보였다(P<0.001). 양성 항체가를 보인 피험자의 비율은 전체 대상의 68.2%였고, 11세 미만 소아 연령군(76.5%)이 가장 높은 비율을 보였다(P<0.001). 결론: 소아 연령군과 비교하여, 청소년과 성인 연령군에서 백일해에 대한 낮은 기하 평균항체가를 나타냈으며 양성 항체가를 갖는 피험자의 비율이 낮다는 것을 확인하였다. Purpose: We conducted the immunoassay of pertussis according to ages, in order to evaluate protective immunity against pertussis in Korean populations. Methods: Healthy subjects were enrolled at four university hospitals in Korea. The subjects were grouped as seven age groups (every 10 years). Antibodies against pertussis toxin (PT) in sera were measured by enzyme linked immunosorbent assay (ELISA) kits. Geometric mean concentrations (GMC) of antibodies and the ratios of the subjects with seroprotective antibody levels were determined. The subjects with antibody titers ${\geq}24.0EU/mL$ were considered to seroprotective as the manufacturer's protocol. Results: Total 1,605 subjects (age: 2 months-65 years) participated in this study, and their GMC was $56.16{\pm}50.54EU/mL$. Among seven age groups, age group <11 year showed the highest GMC ($64.78{\pm}53.24EU/mL$) (P<0.001). In the analysis of the ratios of the subjects with seroprotective antibody titers, 68.2% of the subjects were proven to seroprotective, and age group <11 year also showed the highest ratio (76.5%) (P<0.001). Conclusions: We found that adolescences or adults (age group ${\geq}11$ year) showed lower levels of antibody against pertussis and lower ratio of the subjects with seroprotective antibody titers than children (age group <11 year).

알파 태아단백을 분비하는 원발성 폐암

김영욱 ( Young Wook Kim ),김주상 ( Ju Sang Kim ),안중현 ( Joong Hyun Ahn ),유지현 ( Ji Hyun Yu ),유진석 ( Jin Sok Yu ),전한희 ( Han Hee Chung ) 대한결핵 및 호흡기학회 2012 Tuberculosis and Respiratory Diseases Vol.72 No.1

We observed a very rare case of primary lung cancer producing alpha-fetoprotein (AFP). A 70-year-old male with a history of smoking 50 packs per year was diagnosed with large cell carcinoma of the lung. The clinical stage was T2bN3M0 (IIIB), and serum AFP was 23,247 ng/mL. There was no evidence of metastasis to the liver, scrotum or other organs. While undergoing chemotherapy for 1 year, as the cancer progressed the AFP value steadily increased. The patient died of respiratory failure due to pneumonia 12 months after being diagnosed with lung cancer.

유전자조작, 균주분리 항산화물질을 생산하는 해양방선균 Streptomyces sp. ACT-18의 동정 및 Electron Spin Resonance Spectrometry를 이용한 항산화활성

김만철 ( Man Chul Kim ),김주상 ( Ju Sang Kim ),( Ramasamy Harikrishnan ),한용재 ( Yong Jae Han ),허문수 ( Moon Soo Heo ) 한국미생물생명공학회 2010 한국미생물·생명공학회지 Vol.38 No.1

밀폐형 냉각탑용 열교환기에서의 물질전달

유성연(Seong-Yeon Yoo),김진혁(Jin-Hyuck Kim),한규현(Kyu-Hyun Han),김주상(Joo-Sang Kim),유해성(Hae-Sung Ryu),박형준(Hyoung Joon Park) 대한설비공학회 2009 대한설비공학회 학술발표대회논문집 Vol.2009 No.-

The use of cooling towers in the air conditioning systems of buildings is increasing. In closed wet cooling towers, the heat transfer between the air and surface tubes can be composed of the sensible heat transfer and the latent heat transfer. The latent heat transfer is affected by the air and spray water. This study provides a designing methodology of heat exchanger for closed wet cooling tower. The correlation equation was derived to interpret the mass transfer coefficient based on the analogy of the heat and mass transfer and the experimental results. The results from this correlation equation showed fairly good agreement with experimental data.

제주 연안 퇴적층에서 분리된 미생물의 동정 및 단백질분해효소 특성

김만철(Man-Chul Kim),장태원(Tae-Won Jang),김주상(Ju-Sang Kim),한용재(Yong-Jae Han),Ramasamy Harikrishnan,한송헌(Song-Hun Han),오덕철(Duck-Chul Oh),허문수(Moon-Soo Heo) 한국생명과학회 2009 생명과학회지 Vol.19 No.6

제주도 양식장 배출수 퇴적층에서 단백질 분해 효소를 생산하는 세균을 분리하였으며, 각각 SK-2, 및 SK-125라고 명명하였다. 분리균주 SK-2의 16S rDNA의 염기서열 분석 결과 Bacillus licheniformis와 Bacillus subtillis의 염기서열과 99%의 상동성을 보였으며, BIOLOG를 이용한 생화학적 분석에서도 Bacillus family와 유사한 특성을 보여 최종적으로 분리균주 SK-2를 Bacillus sp. SK-2으로 명명하였다. 또한 분리균주 SK-125의 16S rDNA의 염기서열 분석 결과 Pseudoalteromonas haloplanktis의 염기서열과 99%의 상동성을 보였으며, BIOLOG를 이용한 생화학적 분석에서도 Pseudoalteromonas family와 유사한 특성을 보여 최종적으로 분리균주 SK-125를 Pseudoalteromonas sp. SK-125라고 명명하였다. Bacillus sp. SK-2와 Pseudoalteromonas sp. SK-125균주의 온도별 실험에서는 40℃에서 가장 높은 생육도를 보였으며, 효소활성은 Bacillus sp. SK-2와 Pseudoalteromonas sp. SK-125 두 균주 모두 30℃에서 가장 높은 활성을 보였다. In this study, protease-producing bacteria were isolated from the marine sedimentary layer in coastal Jeju. We isolated 2 protease producing strains (SK-2 and SK-125) and tested their protesase producing activities. Gram staining and BIOLOG of isolated strains revealed that strains SK-2 and SK-125 belong to Bacillus and Pseudoalteromonas families, respectively. The 16S rDNA nucleotide sequences analyses of the isolated strains showed 99% sequence homology with those of Bacillus sp. and Pseudoalteromonas sp.; therefore, the isolated strains SK-2 and SK-125 were named Bacillus sp. SK-2 and Pseudoalteromonas sp. SK-125, respectively. The optimum conditions for the cell growth of protease activities were obtained when the both isolates were cultured at 30℃, 96 hrs and pH 7~8.

제주도내 양식장 배출수의 이화학적 특성 분석

김만철(Man-Chul Kim),장태원(Tae-Won Jang),한용재(Yong-Jae Han),김주상(Han, Ju-Sang Kim),Ramasamy Harikrishnan,오덕철(Duck-Chul Oh),김기영(Ki-Young Kim),허문수(Moon-Soo Heo) 한국생명과학회 2009 생명과학회지 Vol.19 No.7

Physical and chemical analyses of water discharged from 4 crowded farms (Sungsan, Pyosun, Wimi and Daejung) in Jeju island were performed from July, 2006 to Dec, 2006, and the result of the analyses showed that hydrogen ion concentrations (pH) for water discharged from Sungsan farm was 7.74, Pyosun was 7.68, Wimi was 7.68 and Daejung was 7.7. Salinity levels for Sungsan, Pyosun and Wimi had an average of 31~33 ‰, indicating characteristics of far distance areas, whereas that of Daejung was 28.81%, which was far lower compared to regular sea water salinity. As the result of measuring dissolved oxygen (DO) for each area, each area showed first graded DO for each discharged water based on water quality level for each sea district. The result of measuring the temperature for discharged water showed that water temperatures for summer were 23~25℃, and those for winter were 16~18℃. Nitrogen concentrations for discharged water exceeded each sea area's water quality level in all farms. In the case of phosphate, its average value was 0.48 ㎎/ℓ for Sungsan, 0.55 ㎎/ℓ for Pyosun, 0.66 ㎎/ℓ for Wimi, and 0.44 mg/l for Daejung, and chemical oxygen demand (COD) was shown to be 1.5 ㎎/ℓ, 1.8 ㎎/ℓ, 1.6 ㎎/ℓ and 2.3 ㎎/ℓ for Sungsan, Pyosun, Wimi and Daejung respectively. For suspended solids (SS), the average concentration was 19.3 ㎎/ℓ, 21.2 ㎎/ℓ, 21.3 ㎎/ℓ and 18.5 ㎎/ℓ for Sungsan, Pyosun, Wimi and Daejung respectively. The results of physical and chemical analyses for discharged water in farms based on time showed that almost all items were shown to increase in the forenoon and decrease, overall, in the afternoon.