이온주입과 자기유변연마를 복합한 미세가공공정

김종욱(J. W. Kim),한진(J. Han),민병권(B. -K. Min),이상조(S. J. Lee) 한국정밀공학회 2008 한국정밀공학회 학술발표대회 논문집 Vol.2008 No.6월

충돌형 Quadlet 인젝터의 연소성능 예측에 관한 실험적 연구

김종욱(J.W. Kim),박희호(H.H. Park),한재섭(J.S. Han),김선진(S.J. Kim),김유(Y. kim) 한국추진공학회 1999 한국추진공학회지 Vol.3 No.4

한우비육에 관한 연구 3 . Hexestrol Dicaprylate 처리가 한모우비육에 미치는 영향

김창기 ( C K Kim ),이택원 ( T W Lee ),김종욱 ( J W Kim ),배대식 ( D S Bae ),오선균 ( S K Oh ),조지훈 ( C H Cho ),김법회 ( B H Kim ),김상렬 ( S Y Kim ),배신석 ( S S Bae ) 한국축산학회 1972 한국축산학회지 Vol.14 No.3

The effects of hexestrol dicaprylate (HESC) implantation on rate of gain and economic feed utilization were studied. Ten Korean native yearling bulls and a corresponding number of controls were used in this experiment. All animals were fed a fattening ration of soiling corn and concentrate made up of 50% corn, 48% brans and 2% others for 105 days. Implanting with 100㎎ of HESC increased rate of gain by 0.6%. Feed required per 1㎏ of gain was decreased by the implanting of HESC. Implanting with HESC increased rate of nutrient utilization for 1㎏ of body gain by 6.6% DCP and 6.9% TDN. No undesirable effects was noted in the experiment.

밍크정자에 있어서의 효소분포

김종욱,M . S . 아마드 . W . D . 킷츠 . C . R . 크리쉬나머터 ( J . W . Kim,M . S . Ahmad,W . D . Kitts,C . R . Krishnamurti ) 한국축산학회 1977 한국축산학회지 Vol.19 No.4

Epididymal spermatzea of mink were used to study the ceallurar localization of several enzymes. Very strong acid phosphatase activity was found in the equatorial region of the head and tail(middle and principal pieces) of spermatozoa while strong activity was found in the galea dapitis, acrosome, and postacrosomal sheath. Strong alkaline phosphatnse activity was detected in the galea capitis acrosome, post-acrosomal sheath and tail. The activity of glucose-o-phosphatase and 5-nucleotidase were confined to the middle piece. The reaction of glucoseo-phosphatase was very strong while that of 5-nucleotidase appeared weak. The activity of ADPase and ATPase was distributed strongly in the tail and it was localized weakly in the acrosome and postacrosomal sheath. The galea capitis was weakly stained by ATPase. Most phosphatases were localizd in the acrosome postacrosomal sheath, and tail, with the exception of glucose-6-phosphatatase and 5-nucleotidase which were confined to the middle piece. Weak non-specific esterase activity was located in the base of the head and middle piece. The activity of DOPA oxidase was weakly spread in the acrosome, postacrosomal sheatin, and tail. Although the activity of malate dehydrogenase was distribueted in the base of the head and tail, the rest of the dehydrogerase activity examined (succinate, lactate, and isocitrate dehydrogenases except 6-phosphogl accnic dehydrogenase) was confined to the middle piece. The activity of succinate was the strongest among the examined dehydrogenases. The activity of NADH diaphorase was strongly confined to the middle piece. The metabolic enzymes (glueose-6 phosphatase, dehidrogeanses, and NADH diaphorase) were confined to the middle piece, while the activity of malate clehydrogenase was found so be extended to the head base and base and principal piece. The lytic enzymes, acid phosphatase and esterase were localized in the acrosomal portion.

밍크정자의 미세구조

김종욱,M . S . 아흐마드 . W . D . 킷츠 ( J . W . Kim,M . S . Ahmadd,W . D . Kitts ) 한국축산학회 1978 한국축산학회지 Vol.20 No.1

The subcellular structure of epididymal spermatozoa obtained from standard dark mink was studied by means of electron microscopy, using thin-sectioning techniques. The spermatozoan head was seen to be dorsoventrally flattened and ovate in outline. The anterior two-third of the nucleus was covered with the acrosome which can be divided into three segments (apical, man and equatorial) according to the acrosomal content. The posterior one-third was covered with the postaucrosomal sheath which was a dense layer deposited on the inner aspect of the cell membrane. On the dorsal and ventral and ventral and ventral aspects of the bead, six swellings were observed; two of these were located at each side of the proximal border of the equatorial segment, and one was associated with the pestacrosomal region on each side. The cell membrane adhered firmly to the tip of the acrosome and to the postacrosomal sheath, but was found to be usually separated from the rest of the acroseme. The articular structure of the neck appeared to show separate dorsal and ventral plates of the capitulum which were followed by a ring of striated columns of the connecting piece. These striated columns were followed by two major and five minor columns which appeared io continue with the nine dense fibers of the axial fiber bundles. Beneath the inner surface of the capitulum, the proximal centriole wes found in the center of the connected ring. The spermatozoan tail displayed a 9+9+2 pattern in the organization of the axial fiber bundle consting of the dense fibers, double microtubules, and a central pair. The dense fibers number 9, 1, 5, and 6 were larger in diameter than the rest of the dense fibers. In the axonemal complex, the diameter of subfiber A was larger than the central fiber, while that of subfiber B was the smallest. The middle piece was of medium length compared with other mammalian spermatozoa. The annulus was triangular in longitudinal sections and contained nonhomogeneously distributed electron dense material. The fibrous sheath had longitudinal columns. Although the end piece maintained the 9+2 pattern, it did not have the fibrous sheath.

한우 비육에 관한 연구 1 . Methylthiouracil 첨가 및 Estradiol 17β - Cypionate 주사가 약령 모우 비육에 미치는 효과

김창기 ( C K Kim ),이택원 ( T W Lee ),김종욱 ( J W Kim ),배대식 ( D S Bae ),오선균 ( S K Oh ),조지훈 ( C H Cho ),김법회 ( B H Kim ),김상렬 ( S Y Kim ),배신석 ( S S Bae ) 한국축산학회 1971 한국축산학회지 Vol.13 No.1

This study was performed to affirm any effects of methylthiouracil and estradiol 17β-cypionate administered to young bulls for short term fattening. Eighteen bulls of Korean native breed, approximately 1.5 to 2 years of age and 260㎏ to 360㎏ in weight, were used in this study. All bulls had received a basal ration of soiling corn and concentrate made up of 55% grains, 40% brans and 5% others, in a period of 40 days. Bulls were divided into six groups as follows: group C was not treated, group M-2 received 2g of methylthiouracil per day, group M-3 received 3g of methylthiouracil per day, group EM-0 was injected intramusculary with 25㎎ of estradiol 17β-cypionate in the neck region 10 days after the beginning of the fattening period, group EM-2 was injected with estradiol 17β-cypionate as above mentioned and simultaneously received 2g of methylthiouracil per day, and group EM-3 was injected with estradiol 17β-cypionate as above mentioned and simultaneously received 3g of methylthiouracil per day. The results obtained were summarized as follows. 1. The average daily gain was for group C: 0.83㎏, group M-2: 1.07㎏, group M-3: 1.40㎏, group EM-0, 0.93㎏, group EM-2: 0.95㎏, and group EM-3: 1.18㎏. The M-3 group gained significantly(P$lt;0.05) more weight than C and EM-0 group. There were no significant differences in the average daily gain between the groups injected with estradiol 17β-eypionate and the control group. 2. The average daily concentrate intake was as follows: group C: 5.47㎏, group M-2: 5.00㎏, group M-3: 4.52㎏, group EM-0: 5.21㎏, group EM-2: 4.35㎏, and group EM-3: 4.61㎏. The consumption of concentrate was decreased by 17% in the M-3 group compared with the C group. There was no significant difference in the soiling corn intake among these groups. 3. Feed consumed for 1㎏ gain were decreased by the supplementation of methylthiouracil. The consumption of DCP for 1㎏ gain was in group C: 0.82㎏, group M-2: 0.59㎏, group M-3: 0.41㎏, group EM-0: 0.70㎏, group EM-2: 0.59㎏, and group EM-3: 0.50㎏. The amounts of TDN required were; group C: 6.57㎏, group M-2: 4.76㎏, group M-3: 3.39㎏, group EM-0: 5.67㎏, group EM-Z: 4.87㎏, and group EM-3: 4.08㎏. The consumption of DCP and TDN for 1㎏ gain in the M-3 group was about a half of that in the C group. 4. There was not any significant difference in the increase in body measurements among the various groups. 5. The average margins in the fattening period of 40 days were for group C: 2,950won, group M-2: 5,327won, group M-3: 9,158won, group EM-0: 3,310won, group EM-2: 3,623won, and group EM-3: 5,575won. The margin of group M-3 was about three times higher than that of group C. In short, this experiment demonstrated that methylthiouracil when fed to young native Korean bulls at the proper level 40 days before slaughter would bring a noticeable effect on weight gain, feed efficiency and economic advantage. There were no advantages from the simultaneous injecting estradiol 17β-cypionate with methylthiouracil. The proper supplementation level of methylthiouracil would appear to be 3g per head per day in this experiment.

한우비육에 관한 연구 2 . 방목시 Hexestrol Dicaprylate 처리가 Aberdeen Angus (♂) × 한우 (♀) 거세우의 비육에 미치는 효과

김창기 ( C K Kim ),이택원 ( T W Lee ),김종욱 ( J W Kim ),배대식 ( D S Bae ),오선균 ( S K Oh ),조지훈 ( C H Cho ),김법회 ( B H Kim ),김상렬 ( S Y Kim ),배신석 ( S S Bae ) 한국축산학회 1972 한국축산학회지 Vol.14 No.2

The effect of hexestrol dicaprylate implantation on the rate of gain of Aberdeen Angus(♂)×Korean native cattle (♀) F₁ steers were studied. Three steers were implanted with 100㎎ of hexestrol dicaprylate and 3 steers was not treated. All steers grazed native pasture with feeding supplements for 105 days. The average daily gain of the hexestrol implanted steers were more by 9.5% than that of the controls. However, there was no significant difference in daily gain between the groups. The daily gain in the present study were found to be less than that of the young bulls of Kim, et al. (1971). No undesirable side-effects such as development of mammary gland resulted from hexestrol implantation.

반추 가축의 요소 이용에 대한 황산태유황의 효과

김종욱 ( J W Kim ) 한국축산학회 1970 한국축산학회지 Vol.12 No.4

In vivo and in vitro techniques were used to determine the effects of added sulfate-sulfur in urea supplemented rations on the utility of feed constituents in ruminant. Goats were fed with the experimental rations constituted of different ratios of rice straw and corn, and different levels of ammonium sulfate for the metabolic experiment. Significant increase in digestible energy intake (P$lt;0.05) nitrogen and sulfur retention (P$lt;0.05) and improved crude fiber digestibility were obtained when ammonium sulfate was added to the rations which were mainly constituted of the rice straw. On the contrary when ammonium sulfate was added to the rations which were mainly constituted of corn, goats retained less nitrogen (P$lt;0.05). The inoculum taken from a wether fed with common ration was inoculated into the incubation vessels containing rice straw or corn as the substrate, urea and mineral solution (sulfate-sulfur concentration: 4㎎/㎗). Synthesis of protein with sulfate-sulfur or sulfur-containing amino acid in the incubation vessels was observed by using ^(35)S as a tracer, with or without sulfur-containing amino acid, after 12 and 16 hours of the incubation in case of substrate being rice straw and 4 and 8 hours in case of corn. More sulfate-sulfur was incorporated into synthesized protein in case of substrates being rice straw than in case of corn. The results of the studies in vivo and in vitro suggested that nutritional requirements were changed by the change of microorganism pattern according to substrates or basal rations.

우유중 Progesterone 분석에 의한 조기임신진단에 관한 연구

이종문(J . M . Lee),양보석(B . S . Yang),정진관(J . K . Jung),김종욱(J . W . Kim) 한국축산학회 1990 한국축산학회지 Vol.32 No.12

The early pregnancy diagnosis was carried out to improve the reproductivity of dairy cattle by the measurement of progesterone concentration in the milk. The whole or strip milk samples for the determination of progesterone concentration by the radioimmunoassay were taken from seventy-live pregnant or nonpregnant dairy cows. 1. The concentration of progesterone in the milk of pregnant cows was 13.8 n mol/ml on day 9 of pregnancy and increased continuousely until day 23 of pregnancy, but in open cows it decreased from l7th day after the estrus and it was 0.5-0.6 n mol/ml in 21-23 day after the estrus. 2. The precision of pregnancy diagnosis on 19-23 days of pregnancy was 72.6%-84.3% for the pregnant cows and 100%, for open cows. 3. The precision of pregnancy diagnosis by milking method was 79.0-89.1% in whole milk and 81.3-100% in strip milk.

비비 ( Papio anubis ) 의 정자완성에 관한 전자현미경학적 연구

김종욱 ( J . W . Kim,B . A . Afzelius,L . Ploen ) 한국축산학회 1982 한국축산학회지 Vol.24 No.5

The ultrastructure of spermiogenesis of the olive baboon (Papio anubis) has been studied. The four main (Golgi, cap, acrosome and maturation) phases of spermiogenesis are described, and these phases have been further subdivided into a total of ten phases. Spermiogenesis was found to be basically similar to That of other mammalian species, in particular to that of man. A lower number of morphologically deviating cells was found in the baboon, but in other respects most differences between human and baboon spermiogenesis refer to dimensions. Aspects of the morphogenesis of certain structures of the tail are discussed in more detail.