2002년도 한국어류학회 춘계학술발표 : 포스터발표 ; 풀망둑(농어목, 망둑어과)의 전체 미토콘드리아 DNA의 염기서열 분석

김일찬,이용성,이재성 한국어류학회 2002 韓國魚類學會誌 Vol.14 No.2

어류 metallothionein 유전자 : Conserved primer를 이용한 증폭 및 염기서열 분석 Amplification with Conserved Primers and Sequence Analysis

김일찬,김영자,김문규,윤용달,이재성 한국어류학회 2003 韓國魚類學會誌 Vol.15 No.3

수계 환경 오염이 어류에 미치는 영향을 조사하기 위해, 여러 종류의 어류(풀망둑 Acanthogobius hasta; 조피볼락, Sebastes schlegeli;동사리, Odontobutis platicephala;송사리, Oryzias latipes)에서 중금속 오염에 민감하게 반응하는 metallothionein(MT) 유전자를 polymerase chain reaction(PCR)을 이용하여 증폭하였고, 이들의 염기서열을 분석하였다. 조사한 대다수의 어류에서 MT 유전자를 특이적으로 증폭할 수 있었지만, 송사리의 경우 비특이적으로 증폭된 band도 있었다. 본 논문에서는 환경오염의 실험동물로 쓰일 수 있는 종을 중심으로 MT유전자를 증폭하였고 모든 어종에서 MT 유전자의 증폭이 이루어지지 않았으나, 다양한 목에서 같은 conserved primer로 MT유전자가 증폭될 수 있음을 보여 주었다. To investigate the effect of heavymetal contamination in aquatic environments, we amplified and sequenced the metallothionein (MT) gene, a sensitive biomarker, in some fishes. In most of the species tested (medaka, Oryzias latipes; javeline goby, Acanthogobius hasta; black rockfish, Sebastes schlegeli), we cloned the MT gene with a single band, but in a few cases (e.g., medaka), it was difficult to get the specific band for the MT gene. The fish MT gene was isolated with emphasis on several fish species that are candidates for environmental toxicology testing, but in a few cases, it was not successful. However, the PCR primers for this fish-specific MT gene worked properly even among fishes of different phylogenetic orders, although it did not always specifically amplify the MT gene.

Molecular Cloning and Expression of the Mitochondrial 60-kDa Heat Shock Protein from an Arctic Copepod Calanus glacialis

김일찬,이창은,조현희,홍순규,이진성,이형석,이홍금 한국유전학회 2008 Genes & Genomics Vol.30 No.2

Heat shock proteins (Hsps) play an essential role in cell defense against cell damages by several environmental and physiological stresses. We cloned and sequenced full-length heat shock protein 60-kDa (Hsp60) from the Arctic copepod Calanus glacialis cDNA. The complete nucleotide sequence of Calanus glacialis Hsp60 ( CgHsp60) gene contained 2,092 bp in length. The open reading frame (ORF) was 1,743 bp flanked by a 5'-UTR of 90 bp and a long 3'-UTR of 259 bp, and encoded a putative protein of 581 amino acids with a calculated molecular weight of 62 kDa. It showed high similarity to those of Culicoides variipennis (73%), Aedes aegypti (72%), and Tribolium castaneum (71%) at the amino acid sequence level. In phylogenetic analysis, it clustered with other arthropods sequences. We also confirmed that the recombinant protein of C. glacialis Hsp60 by the SDS-PAGE and immunoblotting analysis after expression of recombinant gene construct in Escherichia coli. Heat shock proteins (Hsps) play an essential role in cell defense against cell damages by several environmental and physiological stresses. We cloned and sequenced full-length heat shock protein 60-kDa (Hsp60) from the Arctic copepod Calanus glacialis cDNA. The complete nucleotide sequence of Calanus glacialis Hsp60 ( CgHsp60) gene contained 2,092 bp in length. The open reading frame (ORF) was 1,743 bp flanked by a 5'-UTR of 90 bp and a long 3'-UTR of 259 bp, and encoded a putative protein of 581 amino acids with a calculated molecular weight of 62 kDa. It showed high similarity to those of Culicoides variipennis (73%), Aedes aegypti (72%), and Tribolium castaneum (71%) at the amino acid sequence level. In phylogenetic analysis, it clustered with other arthropods sequences. We also confirmed that the recombinant protein of C. glacialis Hsp60 by the SDS-PAGE and immunoblotting analysis after expression of recombinant gene construct in Escherichia coli.

극지 바이오신소재 상용화 구축 사업

김일찬(Il-Chan Kim),김기태(Kitae Kim),김진형(Jin-Hyoung Kim),이준혁(Lee, Jun Hyuck) 극지연구소 2023 극지연구소 연보 Vol.- No.-

남극장보고과학기지

김일찬(Kim, Il chan) 극지연구소 2022 극지연구소 연보 Vol.- No.-

어류를 이용한 내분비계 장애물질 검출 및 Biomarker로서 Vitellogenin의 이용

윤석주,김일찬,윤용달,이재성 한국육수학회 2003 생태와 환경 Vol.36 No.2

어류 vitellogenin은 난황전구체로 난형성과정동안에 estradiol의 체내 순환에 의해 암컷의 간에서 생산되며 alkylphenol류와 같은 비이온성 계면활성제 등의 내분비계 장애물질에 의해 수컷에서도 생산된다. 물 환경의 주요 생물종인 어류는 내분비계장애물질에 의해 암컷에서는 번식률 저하와 함께 수컷에서는 정소의 축소 및 이에 따른 암컷화가 관찰된다. 특히 수컷에서 내분비계 장애물질에 의해 유도된 vitellogenin의 생성을 이용하여 환경오염에 의해 유도된 생물체의 유전자 발현 변화 뿐만 아니라 이를 토대로 특정지역의 환경오염을 모니터링할 수 있다. 본 논문에서는 어류의 vitellogenin를 이용하여 수환경의 내분비계장애물질의 검출과 환경오염 모니터링을 위한 biomarker로서의 유용성을 검토하였다. Fish vitellogenin produces in female liver during oogenesis through estradiol cycle, and produces even in male liver by endocrine-disrupting chemicals (EDCs) such as alkylphenols. The resulting effects of EDCs lead to the low fecundity of female and the feminization (eg. shrinkage of testis) in male. Especially, the production of vitellogenin in male indicates the environmental contamination of EDCs, resulting in the modulation fo gene expression profiles and the monitoring of environmental contamination at specific area. In this paper, we suggest that fish vitellogenin is useful for biomonitoring for environmental contamination and would be substantially useful as a biomarker for a detection of EDCs in aquatic environment.

수서 환경독성 평가와 어류 Cytochrome P450 1A(CYP1A) 유전자

윤석주,김일찬,윤용달,이재성 한국환경생물학회 2003 환경생물 : 환경생물학회지 Vol.21 No.1

CYP1A 유전자는 cytochrome P450 약물대사효소에 속하며 다이옥신 등의 내분비계 장애물질에 의해 농도 의존적으로 유도되어 환경오염물질에 의해 유도되는 특성을 이용하여 환경오염물질에 대해 반응하는 생물체의 유전자 발현 변화 뿐만 아니라 이를 토대로 특정지역의 환경오염에 관한 정보를 얻을 수 있다. 본 논문에서는 수서환경오염과 관련하여 CYP1A 유전자의 응용성을 다 각도로 논의하였다. The CYPlA gene is one of Cytochrome P450 drug-metabolizing enzymes with dose-dependant manner of gene expression and is useful to get the information of alterations on gene expression upon environmental contaminants as well as the biomarker of environmental contamination at the specific places. In this report, we further discuss the usefulness of CYPlA gene in relation to aquatic environmental contamination at several aspects.

한국산 어류 미토콘드리아 DNA의 분자계통학적 이용 및 보존

김영자,김일찬,이세영,이완옥,조용철,이재성 한국육수학회 2003 생태와 환경 Vol.36 No.3

종다양성 규명을 위한 계통분류학적 연구는 원시형질의 상동성에 근거한 기존의 형태형질과 효율적인 새로운 분자계통분류학적 연구방법이 병행된다면 좀 더 정확한 분류 및 계통을 추적하는데 객관적인 결론을 제시할 수 있을 것이다. 현재 활발히 연구되고 있는 미토콘드리아 DNA를 이용한 분자계통분류학적 연구는 기존의 형태적 특징을 근간으로 한 계통분류를 재검토할 뿐 아니라 계통진화학상 조상과 자손과의 관계를 유추하는데 새로운 가설을 세울 수 있는 토대를 제공하는 기초자료로서 이용될 수 있다. Phylogenetic studies would clarify the diversity of fishes if the morphological analysis based on plesimorphy characters combined with new genetic analysis on molecular level, inferring more accurate and objective phylogeny and the taxonomy. Current molecular phylogenetic approach using mitochondrial genome provides the framework for a new hypothesis not only inferring the relationships between ancestor descendants but raveling the intra-, interspecies variation.

춘계학술대회 요약 : 심포지움 ; 어류 계통분류학의 새로운 동향 : 2. 어류 mitochondrial DNA의 분자계통학적 이용과 국내에서의 분류학적 적용현황

김영자,김일찬,이세영,이완옥,이재성 한국어류학회 2003 韓國魚類學會誌 Vol.15 No.2

자외선에 의한 조류기원 용존유기물의 생분해도 및 화학조성변환

김영자,김일찬,이세영,이완옥,조용철,이재성 한국하천호수학회 2003 생태와 환경 Vol.36 No.3

The effect of ultraviolet (UV) radiation on thecharacteristics of algae-derived dissolved organ-ic matter(DOM) was examined by comparing thebiodegradability and DOM fraction distributionof algal DOM before and after UV exposure. Al-gal DOM from two axenic cultures of Microcystisaeruginosa and Oscillatoria agardhii were irra-diated for 24 h at a UV intensity of 42 W/m2. Acomplete degradation of algal DOM during theUV exposure did not occur, remaining at cons-tant concentrations of dissolved organic carbon(DOC). After UV exposure, however, microbialdegradations were reduced by 17% in M. aerugi-nosa and 53% in O. agardhii , respectively, anddecomposition rates also were two times lower inUV exposed algal DOM. In addition, the chemi-cal compositions of algal DOM altered substan-tially after UV radiation exposure. The propor-tions of hydrophilic bases (HiB; protein-likeDOM) decreased considerably in both algal DOMsources after UV exposure (16.8% and 20.0% ofDOM, respectively), whereas those of hydrophilicacids(HiA; carboxylic acids-like DOM) increasedas much as the decrease of the HiB fraction.Capillary ion electrophoresis (CE) analysis show-ed that several carboxylic acids increased signi-ficantly after UV exposure, further confirmingan increase in HiA fractions. The results of thisstudy clearly indicate that algal DOM can bechanged in its chemical composition as well asbiodegradability without complete degradationby UV radiation.ACKNOWLEGDMENTSThis work was supported by postdoctoral fel-lowship program from Korea Science and Engi-neering Foundation (KOSEF). We would like tothank Dr. Robert G. Wetzel, University of NorthCarolina, for his helpful review and comment onthe manuscript. We also thank Dr. NobuyoshiNakajima, National Institute for EnvironmentalStudies, and Masaya Ueki, Kyoto University fortheir helping us with measurements with thespectroradiometer and CE system.