和의 政治思想과 그 現代的 役割

金宇寧 대구효성가톨릭대학교 1996 연구논문집 Vol.53 No.1

The most important life-style of the morden society is the pronciple of competition. The principle of competition was generated by the growth of liveralism. Of course, such a thought was enjoyed liberty of each and everyman with the development of material civilization, but, Otherwise, such a condition watched human relation with violent competition. I think such a developing is life-style that the principle of competition bring to two axes like liberalism and utility. If we are to speak, modern society have a single line of value system, utility deciding liveral catefory, but, on the contrary, the traditional principle of harmony was considered the life-style is very important to the all-embracing value system. Modern society enjoy material abundance, but happen several cocial invalidism, and so, now is a point of time that the life-style of competition will be under revision. Therefore, on this paper, I want to consider modern role of the principle of harmony in the traditional political thoughts of Korea. The principle of harmony embrace perfect four virtues(virtue, righteousness, courtesy, wisdom) and perpetually unmoved in the mind. But, the reason, inner rhythm of liveral category in the western society does not embrace perfect virtue, courtesy and perpetually unmoved in the mind. And then I considered the principle of harmony all-embracing unmoved in the mind is possible to settle Anomie problem, class conflict and the problem of welfare state and so on.

Expression Analyses of MicroRNAs in Hamster Lung Tissues Infected by SARS-CoV-2

김우령,박은경,Kyung-Won Kang,Sang Myeong Lee,Bum-Seok Kim,김희수 한국분자세포생물학회 2020 Molecules and cells Vol.43 No.11

Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is an infectious disease with multiple severe symptoms, such as fever over 37.5°C, cough, dyspnea, and pneumonia. In our research, microRNAs (miRNAs) binding to the genome sequences of severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory-related coronavirus (MERS-CoV), and SARS-CoV-2 were identified by bioinformatic tools. Five miRNAs (hsa-miR-15a-5p, hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-16-5p, and hsa-miR-196a-1-3p) were found to commonly bind to SARS-CoV, MERS-CoV, and SARS-CoV-2. We also identified miRNAs that bind to receptor proteins, such as ACE2, ADAM17, and TMPRSS2, which are important for understanding the infection mechanism of SARS-CoV-2. The expression patterns of those miRNAs were examined in hamster lung samples infected by SARS-CoV-2. Five miRNAs (hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-221-3p, hsa-miR-140-3p, and hsa-miR-422a) showed differential expression patterns in lung tissues before and after infection. Especially, hsa-miR-15b-5p and hsa-miR-195-5p showed a large difference in expression, indicating that they may potentially be diagnostic biomarkers for SARS-CoV-2 infection.

Hsa-miR-422a Originated from Short Interspersed Nuclear Element Increases ARID5B Expression by Collaborating with NF-E2

김우령,박은경,Hee-Eun Lee,Sang-Je Park,Jae-Won Huh,김정남,김희수 한국분자세포생물학회 2022 Molecules and cells Vol.45 No.7

MicroRNAs (miRNAs) are a class of small non-coding RNAs that regulate the expression of target messenger RNA (mRNA) complementary to the 3’ untranslated region (UTR) at the post-transcriptional level. Hsa-miR-422a, which is commonly known as miRNA derived from transposable element (MDTE), was derived from short interspersed nuclear element (SINE). Through expression analysis, hsa-miR-422a was found to be highly expressed in both the small intestine and liver of crab-eating monkey. AT-Rich Interaction Domain 5 B (ARID5B) was selected as the target gene of hsa-miR-422a, which has two binding sites in both the exon and 3’UTR of ARID5B. To identify the interaction between hsa-miR-422a and ARID5B, a dual luciferase assay was conducted in HepG2 cell line. The luciferase activity of cells treated with the hsa-miR-422a mimic was upregulated and inversely downregulated when both the hsa-miR-422a mimic and inhibitor were administered. Nuclear factor erythroid-2 (NF-E2) was selected as the core transcription factor (TF) via feed forward loop analysis. The luciferase expression was downregulated when both the hsa-miR-422a mimic and siRNA of NF-E2 were treated, compared to the treatment of the hsa-miR-422a mimic alone. The present study suggests that hsa-miR-422a derived from SINE could bind to the exon region as well as the 3’UTR of ARID5B. Additionally, hsa-miR-422a was found to share binding sites in ARID5Bwith several TFs, including NF-E2. The hsa-miR-422a might thus interact with TF to regulate the expression of ARID5B, as demonstrated experimentally. Altogether, hsa-miR-422a acts as a super enhancer miRNA of ARID5Bby collaborating with TF and NF-E2.

Expression analysis of LTR‑derived miR‑1269a and target gene, KSR2 in Sebastes schlegelii

임제니퍼,김우령,이희은,김아란,김도형,최영현,차희재,김석만,김희수 한국유전학회 2020 Genes & Genomics Vol.42 No.1

Background Sebastes schlegelii are an important species of fish found in the coastal areas of the Korea with significant commercial importance. Most studies thus far have been primarily focused on environmental factors; behavioural patterns, aquaculture, diseases and limited genetic studies with little to none related to either microRNAs (miRNAs) or transposable elements (TE). Objectives In order to understand biological roles of TE-derived miR-1269a, we examined expression pattern for miR-1269a and its target gene, KSR2, in various tissues of Sebastes schlegelii. Also, we performed luciferase reporter assay in HINAE cells. Methods UCSC Genome Browser (https ://genom e.ucsc.edu/) was used to examine which TE is associated with miR-1269a. For the target genes for miR-1269a, the target genes associated with the miRNA were identified using miRDB (http://www. mirdb .org/) and TargetScan 7.1 (http://www.targe tscan .org/vert_71/). A two-step miRNA kit, HB miR Multi Assay Kit™ System. I was used for the analysis of TE-derived miRNA expression patterns. The 3′UTR of KSR2 gene was cloned into the psiCHECK-2 vector. Subsequently co-transfected with miR-1269a mimics to HINAE cells for luciferase reporter assay. Results MiR-1269a was found to be derived from LTR retrotransposon, MLT2B. LTR-derived miR-1269a was highly expressed in the muscle, liver and gonad tissues of Sebastes schlegelii, but KSR2 revealed high expression in the brain. Co-transfection of KSR2 and miR-1269a mimic to HINAE cells showed high activity of miR-1269a in relation to KSR2. Conclusion LTR-derived miR-1269a showed enhancer activity with relation to KSR2 in Sebastes schlegelii. The data may be used as a foundation for further investigation regarding correlation of miRNA and target genes in addition to other functional studies of biological significance in Sebastes schlegelii.

이동성 유전인자의 구조 및 생물학적 기능

김소원(So-Won Kim),김우령(Woo Ryung Kim),김희수(Heui-Soo Kim) 한국생명과학회 2019 생명과학회지 Vol.29 No.9

이동성 유전인자는 인간 유전체의 45%를 차지하며 기능성 유전자 내부로 자유롭게 들어갈 수 있다. 이들은 진화과정에서 중복현상으로 다수의 복사수로 생성되며, 생물종다양성 및 계통유전체학 분야에 기여한다. 이동성 유전인자의 대부분은 메틸화 또는 아세틸화 현상과 같은 후성유전학적 조절에 의해 제어된다. 다양한 생물종은 그들만의 고유의 이동성 유전인자를 가지고 있으며, 일반적으로 DNA트란스포존과 레트로트란스포존으로 나뉜다. 레트로트란스포존은 LTR의 유무에 따라 다시 HERV와 LINE으로 구분된다. 이동성 유전인자는 프로모터, 인핸서, 엑손화, 재배열 및 선택적 스플라이싱과 같은 다양한 생물학적 기능을 수행한다. 또한 이들은 유전체의 불안정성을 야기시켜 다양한 질병을 유발하기도 한다. 따라서, 암과 같은 질병을 진단하는 바이오 마커로 사용될 수 있다. 최근, 이동성 유전인자는 miRNA를 만들어 내는 것으로 밝혀졌으며, 이러한 miRNA는 타겟 유전자의 seed 영역에 결합함으로서 mRNA의 분해 및 번역을 억제하는 역할을 수행한다. 이동성 유전인자 유래의 miRNA는 기능성 유전자의 발현에 큰 영향을 미친다. 다양한 생물종과 조직에서 서로 다른 miRNA의 비교 분석 연구는 생물학적 기능과 관련하여 진화학과 계통학 영역에서 흥미 있는 연구 분야라 할 수 있겠다. Transposable elements (TEs) occupy approximately 45% of the human genome and can enter functional genes randomly. During evolutionary radiation, multiple copies of TEs are produced by duplication events. Those elements contribute to biodiversity and phylogenomics. Most of them are controlled by epigenetic regulation, such as methylation or acetylation. Every species contains their own specific mobile elements, and they are divided into DNA transposons and retrotransposons. Retrotransposons can be divided by the presence of a long terminal repeat (LTR). They show various biological functions, such as promoter, enhancer, exonization, rearrangement, and alternative splicing. Also, they are strongly implicated to genomic instability, causing various diseases. Therefore, they could be used as biomarkers for the diagnosis and prognosis of diseases such as cancers. Recently, it was found that TEs could produce miRNAs, which play roles in gene inhibition through mRNA cleavage or translational repression, binding seed regions of target genes. Studies of TE-derived miRNAs offer a potential for the expression of functional genes. Comparative analyses of different types of miRNAs in various species and tissues could be of interest in the fields of evolution and phylogeny. Those events allow us to understand the importance of TEs in relation to biological roles and various diseases.

Z-DNA–Containing Long Terminal Repeats of Human Endogenous Retrovirus Families Provide Alternative Promoters for Human Functional Genes

Du Hyeong Lee,배우현,하홍석,박은경,이윤주,김우령,김희수 한국분자세포생물학회 2022 Molecules and cells Vol.45 No.8

Transposable elements (TEs) account for approximately 45% of the human genome. TEs have proliferated randomly and integrated into functional genes during hominoid radiation. They appear as right-handed B-DNA double helices and slightly elongated left-handed Z-DNAs. Human endogenous retrovirus (HERV) families are widely distributed in human chromosomes at a ratio of 8%. They contain a 5′-long terminal repeat (LTR)-gag-pol-env-3′-LTR structure. LTRs contain the U3 enhancer and promoter region, transcribed R region, and U5 region. LTRs can influence host gene expression by acting as regulatory elements. In this review, we describe the alternative promoters derived from LTR elements that overlap Z-DNA by comparing Z-hunt and DeepZ data for human functional genes. We also present evidence showing the regulatory activity of LTR elements containing Z-DNA in GSDML. Taken together, the regulatory activity of LTR elements with Z-DNA allows us to understand gene function in relation to various human diseases.