생쥐 난포의 체외배양 중 생식샘자극호르몬에 따른 미세리보핵산 발현 양상

김용진,구승엽,김윤영,오선경,김석현,최영민,문신용,Kim, Yong-Jin,Ku, Seung-Yup,Kim, Yoon-Young,Oh, Sun-Kyung,Kim, Seok-Hyun,Choi, Young-Min,Moon, Shin-Yong 대한생식의학회 2009 Clinical and Experimental Reproductive Medicine Vol.36 No.4

목 적: 미세리보핵산 (microRNA, miR)은 전사 후 (post-transcriptional) 단계에서 목표 유전자 (target gene)의 발현을 억제하여 세포의 발달과 성장에 중요한 역할을 하는 것으로 알려져 있다. 그러나 난포의 성장과정 중의 miR 발현 양상에 대해서는 잘 알려져 있지 않다. 따라서 존 연구는 생쥐 난포의 체외배양 후 생식샘자극호르몬 (gonadotropin)과 사람융모성생식샘자극호르몬 (hCG) 첨가에 따른 난자와 난구세포에서의 miR 발현 양상을 살펴보고자 수행되었다. 연구방법: 생후 12일된 생쥐 (C57BL6)의 난소 적출 후, 전동 난포 (preantral follicle)를 분리하여 무작위로 $20\;{\mu}L$ 점적의 배양액만 있는 군 (control group), 재조합 난포자극호르몬을 첨가한 군 (FSH group), 재조합 황체형성호르몬을 첨가한 군 (LH group), FSH와 LH를 같이 첨가한 군 (FSH+LH group)으로 나누어 배양하였다. 난포가 충분히 성장하였을 때, 다시 hCG를 첨가한 군 (hCG (+) group)과 첨가하지 않은 군 (hCG (-) group)으로 나누어 hCG (-) group에서 난자와 난구세포를 각각 분리하여 RNA를 추출하였다. 36시간 후, 배란된 난자 난구세포 복합체 (cumulus oocyte complex, COC)에서 난자와 난구세포를 각각 분리하여 RNA를 추출하고, mmu--miR-16, -miR-27a, -miR-126, -miR-721 등의 miR에 대한 primer를 이용하여 실시간 중합연쇄반응을 시행하였다. 결 과: 배란율과 MII 난자 생성율은 다른 군들에 비해 FSH+LH군에서 유의하게 높았다. 각 군내의 난자와 난구세포 사이에서도 miR 발현 양상의 차이가 관찰되었다. 또한, 난자와 난구세포에서의 miR 발현 양상은 각 군간 차이가 있었으며, hCG (+)군과 hCG (-)군간에도 차이를 나타냈다. 결 론: 생쥐 난포의 체외배양 중 난자 및 난구세포에서의 miR 발현 양상은 gonadotropin의 종류 및 난자의 성숙도에 따라 다르다. 이러한 결과는 표적 유전자의 발현에 대한 추후 연구를 통한 확인이 필요할 것으로 사료된다. Objective: MicroRNAs (miR) are known to repress target genes at post-transcriptional level and play important roles in development and maturation of cell. However, the expression profiles of miR during ovarian follicle maturation have not been fully elucidated. Here, we designed this study to investigate the expression profiles of miR in oocytes and granulose cells (G-cells) after in vitro culture according to gonadotropins and adding hCG. Methods: Ovaries from 12-day-old mice (C57BL6) were removed and preantral follicles were isolated and cultured in $20\;{\mu}L$-drop of culture media with supplementation of either rFSH, rLH, or rFSH+rLH. After their full maturation, follicles were incubated with rhCG and rEGF. RNA was isolated from oocytes and G-cells, and real-time PCR were performed with primers of miR known to be expressed in the mouse ovary (mmu-miR-16, -miR-27a, -miR-126, -miR-721). Results: FSH+LH group showed the highest ovulation and MII rates among gonadotropin groups. The profiles of miRs in oocytes and G-cells differed according to gonadotropin groups and adding hCG. The profiles of miRs showed divergent changes between oocytes and G-cells. Conclusion: miR expression profiles are altered by gonadotropins and supplementation of hCG during in vitro maturation of murine follicles. Target gene study must be necessary to validate these findings.

치성 협부 봉와직염의 증상으로 발현된 Sweet 증후군; 증례 보고

김용진,변수환,김준영,안강민,전주홍,이부규,Kim, Yong-Jin,Byun, Soo-Hwan,Kim, Jun-Young,Ahn, Kang-Min,Jeon, Ju-Hong,Lee, Bu-Kyu 대한악안면성형재건외과학회 2007 Maxillofacial Plastic Reconstructive Surgery Vol.29 No.6

Sweet syndrome is characterized by acute onset of fever. neutrophilic leukocytosis, painful erythematous plaque on the face and extremities, infiltration of mature neutrophils in the dermis. Cutaneous lesion and clinical symptoms rapidly improve after treatment with systemic corticosteroids. The cause of sweet syndrome is unknown but the associations with hypersensitivity to bacteria, virus, or tumor antigen have been reported. Sweet syndrome itself can be a premonitory manifestation of malignancy, so diagnostic work up for other internal malignancy is recommended. Because of fever and leukocytosis, cutaneous infections are important differentials. Sweet syndrome can be divided into 4 categories according to associated disease and symptom. (Idiopathic Sweet syndrome, Parainflammatory Sweet syndrome, Paraneoplastic Sweet syndrome, Pregnacy associated Sweet syndrome.) Sweet syndrome is relatively rare disease and the association with myelodisplastic syndrome has been reported. We report a case of Sweet syndrome associated with myelodisplastic syndrome which has initial manifestation of odontogenic buccal cellulites.

비냉각 열상시스템에서의 적외선 검출기의 열전소자(TEC) 부재에 대한 효율적인 제어기법

김용진,서재길,김영길,Kim, Yong-Jin,Seo, Jae-Gil,Kim, Young-Kil 한국정보통신학회 2012 한국정보통신학회논문지 Vol.16 No.10

비냉각 방식의 검출기는 검출기 내부에 냉각기 기능을 하는 열전소자(TEC : Thermal Electric Cooler)를 탑재하여 냉각기의 기능을 대신하고 있다. 이 열전소자의 기능은 검출기의 기준온도를 제어하여 온도변화에 따른 영상화질의 저하를 방지하는 역할을 수행한다. 최근에는 이 열전소자를 삭제하여 크기, 가격을 줄일 수 있는 노력이 많이 연구되고 있다. 본 논문에서는 비냉각 형태의 적외선 검출기는 내부에 열전소자가 없는 검출기를 보다 효과적으로 제어하여 영상의 화질저하를 최소화 하면서 크기를 최소화 하고 가격경쟁력을 높일 수 있는 효율적인 제어기법에 대해 실제 챔버를 활용한 시험결과를 제시하고자 한다. The detector is an uncooled detector system that functions inside the thermoelectric cooler (TEC) equipped with features instead of the cooler. The function of the thermoelectric device to control the temperature of the detector based on a function of temperature to prevent degradation of image quality to perform the role, the latest technology trend by removing the thermoelectric device size, cost a lot of effort to reduce has been studied. In this paper, It would be proposed of the actual test result using real chamber environment of for the best TECless algorithm as to minimize the degradation of image quality and obtain the low price of the uncooled detector.

광-ETherCAT 네트워크 기반 PMSM의 벡터제어 구현

김용진,김광헌,배영철,Kim, Yong-Jin,Kim, Kwang-Heon,Bae, Young-Chul 한국전자통신학회 2013 한국전자통신학회 논문지 Vol.8 No.4

본 논문에서는 로봇에서 다축 모션 제어를 쉽게 할 수 있는 AC 서보 드라이버의 성능 검증을 위하여 벡터 전류 제어 구현 기법을 제안한다. 이를 위해 먼저 PMSM을 구동을 위한 드라이버를 개발한 후 이 드라이버가 정상적으로 동작하는지를 벡터 전류로 확인하였다. 벡터 제어는 무부하에서 전류 벡터 제어를 실행하여 지령 전류에 따른 추종 전류를 비교하였다. 검증 결과 만족할 만한 결과를 얻었음을 확인하였다. In this paper we propose implement technique of vector current control in order to verify performance of an AC servo driver that is able to easy control of motion with multi-axis in the robot. In doing do, we have developed the AC servo driver to driving PMSM, and then we confirm that this driver whether operating or not normally by controlling of vector current. The vector current control was performed at the no load condition in PMSM. Then we compare command control and tracking control. As a result of verification, we recognize we get a satisfactory result.

로봇의 다축 모션 제어용 AC 서보 모터 드라이버 구현

김용진,배영철,김광헌,Kim, Yong-Jin,Bae, Young-Chul,Kim, Kwang-Heon 한국전자통신학회 2012 한국전자통신학회 논문지 Vol.7 No.3

An effort for motion control of multi-axis in robot have been continued recently. In this paper, we propose implementation method for AC servo driver that can be easily motion control of multi-axis in robot. This proposed method implement EtherCAT communication technologies of bi-directional optical communication based on single optical core method that applied WDM for communication between control stage which is upper and AC servo drive stage. 최근에 로봇에서 다축 모션 제어를 위한 노력이 계속되고 있다. 본 논문에서는 로봇에서 다축 모션 제어를 쉽게 할 수 있는 AC 서보 드라이버를 구현 기법을 제안한다. 제안한 방법은 먼저 상위의 제어기 단과 드라이버단 사이의 통신을 위해 WDM 방식을 적용한 싱글 광 코어 기반의 양방향 광 EtherCAT 통신 기술을 적용하여 구현한다.

요골 동맥 표재 수장 분지 유리 피판술을 이용한 수지 연부 조직의 재건

김용진,서영석,이상현,함동길,Kim, Yong-Jin,Suh, Young-Suk,Lee, Sang-Hyun,Hahm, Dong-Gil 대한미세수술학회 2012 Archives of reconstructive microsurgery Vol.21 No.1

The radial artery superficial palmar branch free flap is based on the perforators of the superficial palmar branch of the radial artery and its venae comitantes. This flap can be used as a sensible flap including palmar cutaneous branch of the median nerve. Forty radial artery superficial palmar branch free flaps were performed at Centum Institute during October 2010 to December 2011. There were 32 males and 8 females and their mean age were 48 years (range 30 to 66 years). The thumb injured in 13 patients, the index finger in 16 patients, the middle finger in 4 patients, the ring finger in 2 patients, and the little finger in 5 patients. The mean size of the flap was $2.5{\times}3.5$ cm(range $2{\times}2.5$ to $3{\times}7$ cm). The donor site was always closed primarily. The overall survival rate was 90.2 percent. The flaps showed well-padded tissue with glabrous skin. All patients have touch sensation and showed 12 mm two point discrimination in an average(range 8 to 15 mm). Donor site morbidity was conspicuous. One patient showed unsightly scar. Early postoperative range of motion of the affected thumb showed slightly limited radial and palmar abduction. But it improved after postoperative 2 months, and patients did not complaint limitation of motion. In conclusion, the radial artery superficial palmar branch free flap can be used as an option for soft tissue reconstruction of finger defects where local or island flaps are unsuitable.

씨름선수, 과체중 및 정상체중을 지닌 일반인의 혈청 Leptin 농도의 비교분석

김용진(Young Jin Kim),김순기(Soon Ki Kim),박동호(Dong Ho Park),이신언(Shin Eon Lee) 한국사회체육학회 2008 한국사회체육학회지 Vol.0 No.34

Leptin is known as a hormone released from the adipocytes to regulate weight and energy expenditure. A number of studies have shown that the leptin has correlations with body mass index(BMI), body fat(%), fat-mass, fat-free mass, growth hormone(GH), insulin-like growth factor I(IGF-1), testosterone(T) and exercise. However, these results are inconsistent. So, the purpose of this study was to conform when selecting body composition group(normal vs abnormal) for leptin research, what is more useful standard in the middle of BMI and %body fat. Second to evaluate the effect of long term`s training on leptin levels. Third, finding what are the correlated factors by using the correlation analysis. We select the participants who has not been doing exercise for 1 year(NE, n=16) and ssirum player who has been doing exercise for least 6 years(AT, n=12). Among the NE, we made two groups(normal and abnormal) by using both standard of BMI and %body fat. And the levels of GH, IGF-1, T and leptin were compared between groups. In addition, we select participants who has the equal body composition between NE and AT, and serum leptin concentration was compared. In conclusion, the two standards, BMI and %body fat, made significant differences on the leptin concentration(p<.05) between the groups. The %body fat standard made more different. There was no significant different between AT and NE group at the equal body composition condition(p>05). Only the level of T was significantly correlated(r=-.84, p<.001). We concluded that %body fat standard is more useful when make different body composition group for leptin research. Long term exercise didn`t have an independent effect on leptin concentration, but T has much more effects on leptin.

Anisakis病에 의한 호산구성 ( 好酸球性 ) 장염 ( 場炎 )

김용진(Yong Jin Kim),정진영(Jin Young Jung),김원근(Won Kun Kim),송준화(Jun Hwa Song),김교선(Kyo Sun Kim),김중구(Joong Gu Kim),박하철(Ha Chul Park),이종찬(Jong Chan Lee) 대한소화기학회 1990 대한소화기학회지 Vol.22 No.2

Eosinophilic enteritis is characterized by marked submucosal edema and massive infiltration of eosinophiles throughout the entire intestinal wall. The etiology of this disease is not clear until now, although some authors insisted that some gastrointestinal allepens, parasites or collagen diseases. Authors report the case of eosinophilic enteritis of ileum with embedded larva of Anisakis, which is thought as etiology. The patient is 47-year-old male, who was healthy, received operation due to acute abdominal pain with small bowel obstruction. He lived in Eastern sea side of Korea and liked to eat the raw cuttle fish. The specimen is segmental resected product of ileum and shows central small ulcer lesion and marked submucosal edema. On histiology examination, marked eosinophilice infiltration throughout the mucosa to serosal layer, accompanied with marked submucosal edema. The three segmented body of Anisakis larva is present in mucosa to submucosal area, which site is just correlated with small ulcer lesion on gross examination. We concluded that the possibility of Anisakiasis as etiology of eosinophilic enteritis may be high especially in the cases of sea-side inhabitants. So we think that careful seria1 sections for pathologic examination is needed in such cases.

증례 : 항암 화학 요법 및 방사선 요법으로 장기 생존을 보인 상악동에 생긴 골육종 1예

김용진 ( Yong Jin Kim ),김진영 ( Jin Young Kim ),성희진 ( Hee Jin Seong ),박우영 ( Woo Young Park ),권선영 ( Sun Young Kwon ),도영록 ( Young Rok Do ),송홍석 ( Hong Suk Song ) 대한내과학회 2007 대한내과학회지 Vol.72 No.4

저자들은 광범위 수술적 절제가 불가능한 상악동 부위에 생긴 골육종 환자에 대해 항암화학요법과 방사선 요법을 시행하여 장기 생존을 보인 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Maxillary osteosarcoma is an aggressive disease with a high mortality rate. Extensive surgical resection is accepted as the standard treatment for the disease. The beneficial role of chemotherapy and radiotherapy in the treatment of the disorders is uncertain. We experienced a case of an osteosarcoma of the maxillary sinus. Paranasal sinus computed tomography showed a huge solid mass lesion at the left maxillary sinus walls. An endoscopic biopsy showed an osteoblastic type osteosarcoma. In this case, radical surgery was impossible, and the patient was treated with chemotherapy and radiotherapy. This regimen involved four cycles of chemotherapy, cisplatin, 100 mg/m2 intravenously on the first day of weeks 1, 4, 7, and 10, and doxorubicin, 25 mg/m2 per day on the first 3 days of weeks 1, 4. 7. and 10, followed by external beam radiotherapy with a total dose of 6,600 cGy. We report here a case of an inoperable osteosarcoma of the maxilla with long-term survival after chemotherapy and radiotherapy with an accompanying review of the literature. (Korean J Med 72:437-440, 2007)

IPA를 활용한 농촌중심지 활성화 사업에 대한 거주민 인식 - 장수군 장계면을 중심으로 -

김용진 ( Kim Young-jin ),손용훈 ( Son Yong-hoon ) 한국농촌계획학회 2017 농촌계획 Vol.23 No.3

The purpose of this study is to assess residents' perception of the revitalization projects of the rural center and to evaluate their views of the projects' importance and their levels of satisfaction with how they have been carried out. In this study, we measured the residents' perceptions and preferences regarding these projects by using Importance-Performance Analysis (IPA). Results showed that residents' perceptions and satisfaction were the highest for the projects providing welfare facilities, even though this project has little relevance to the overall project goal. Residents were dissatisfied with construction projects such as the building of cultural facilities and urban parks, even while recognizing their importance. It is necessary to establish what problem the people perceive and to manage them appropriately. Next, the residents did not appreciate the community empowerment projects, nor did they evaluate the contents of the program positively. It can therefore be seen that the community empowerment projects have not been carried out well. Finally, the street landscape improvement project was not successfully implemented compared to other projects. The residents have negatively perceived the installation of facilities that they do not want, rarely use, or are poorly operated. this shows that the evaluation of residents is significant. They become the users of the revitalization project for the rural center. It is necessary to overcome problems such as project items chosen by professionals without local consultation and obvious design in the revitalization project of the local center. There is a need to ascertain the residents' opinion of each project and to encourage them to participate directly in the management operation.