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김영환 ( Y . H . Kim ) 한국축산학회 1989 한국축산학회지 Vol.31 No.8
The sodiumdodecylsulfate-polyacrylamide gel electrophoresis(SDS-PAGE) was used to determine the nonmeat protein contents(Isolated soy protein: ISP, Vital wheat gluten: VWG) in cooked meat/non-meat protein blends. Differentiation and identification of meat species by isoelectric focusing(IEF) in polyacrylamide gel (pH range 3-10) were studied. The fresh meat samples from 2 meat species(beef, pork) were compared. Differential scanning calorimeter(DSC) was applied to analyze the mixing ratio of pork and beef in meat blends (mixing ratios of beef to pork were 1:0.3, 0.6 and 1.0) by studying the heat denaturation properties(Transition temperature and Enthalpy) of stroma proteins. The results obtained were summarized as follows; 1. SDS-PAGE patterns showed protein bands in similar positions, so this method was not reliable for the identification of meat species. Protein from the different meat species may differ little in intensity or sharpness of the bands. However, SDS-PAGE method suggested that meat and non-meat protein in meat blends can be successfully identified and quantitated. 2. IEF band patterns from meat proteins of the different species showed the most distinct differences. 3. The regression equation of the line for peak area ratio(Y) versus protein concentration ratio(X) of ISP/beef protein was found to be Y=1.2860X + 0.0017(r=0.9943). For VWG content, corresponding line was Y=O.7380X +0.0058(r=0.9920). 4. In the case of pork, the regression equation of the line for peak area ratio(Y) versus protein concentration ratio(X) of ISP/pork protein was found to be Y=1.0227X -0.0003(r=0.9952). And the equation with VWG content was Y=0.5452X + 0.0092(r=0.9843). 5. Application of the above equations made the quantitation of ISP and VWG in a meat blend possible within 2% of actual content. 6. With DSC analysis of the mixture of pork and beef, the linear equations of the mixing ratio were calculated as Y=0.51X + 67.30(r=0.997) for transition temperature and Y=0.29X + 0.60(r=0.980) for enthalpy.
김영환(Y . H . Kim),박구부(G . B . Park),이무하(M . H . Lee),김일석(I . S . Kim) 한국축산학회 1987 한국축산학회지 Vol.29 No.9
The stacking sodium dodecylsulfate-acrylamide gel electrophoresis method was used to determine the non-meat protein content in chicken products. Densitograms of the selected protein band, denoted by SI, MI, used for the quantitative estimation of isolated soy protein (ISP) and milk protein (MP), respectively. The equation of the line of best fit fot peak area ratio (y) versus protein concentration ratio (X) of ISP/ chicken protein was found to be ; y = 1.5096X + 0.0163 (r=0.9318). And for MP content the corresponding line of best fit was y=1.3536X - 0.0112 (r = 0.9693). These protein content could be determined within 2% of the true value in the range of 10∼30% ISP and MP respectively.
기판 온도의 영향에 따른 펄스레이저 증착법으로 성장된 ZnO 박막의 발광 특성
김영환,김성일,Kim, Y.H.,Kim, S.I. 한국진공학회 2009 Applied Science and Convergence Technology Vol.18 No.5
펄스레이저 증착법으로 박막의 결함 생성을 최소화하여 우수한 발광 특성을 가지는 ZnO 박막 성장에 대한 연구를 수행하였다. 이를 위하여 기판 온도를 $400^{\circ}C$에서 $850^{\circ}C$까지 변화시켜 박막을 증착한 후 엑스선 회절법, 원자힘 현미경, photoluminescence (PL) 등을 사용하여 박막의 특성 변화를 분석하였다. 그 결과 ZnO 박막은 기판 온도에 관계없이 (0001) 사파이어 기판에 c-축 배향성을 가지며 성장하였음을 확인하였고 기판온도 $600^{\circ}C$에서 가장 조밀한 박막이 형성되면서 박막에 응력이 거의 걸리지 않고 결정성도 우수함을 확인하였다. PL 분석 결과 역시 $600^{\circ}C$에서 증착된 ZnO 박막이 UV 발광 피크의 반치폭 및 결함에 의한 가시영역에서의 발광 등을 고려했을 때 가장 뛰어난 특성을 보여주었다. 이와 같은 결과는 ZnO 박막의 발광 특성이 박막의 구조적 특성과 매우 밀접한 관계가 있음을 나타내며 또한 기판 온도가 매우 중요한 역할을 함을 나타낸다. 결론적으로 기판 온도 $600^{\circ}C$에서 우수한 UV 발광 특성을 가지면서 결함에 의한 가시영역 발광이 거의 나타나지 않는 ZnO 박막을 성장시킬 수 있었고 이러한 박막은 UV 광소자에 응용될 수 있을 것으로 생각된다. We investigated the growth of ZnO thin films with prominent emission characteristics through minimizing the formation of defects by using pulsed laser deposition (PLD). To do so, the ZnO films were deposited on sapphire(0001) substrates at the substrate temperature of $400-850^{\circ}C$ and then the variation of their structural and optical properties were analyzed by x-ray diffraction, atomic force microscope and photoluminescence. As a result, all ZnO films were grown with c-axis preferential orientation irrespective of the substrate temperature. However, the crystallinity and stress state were dependent on the substrate temperature and the ZnO film deposited at $600^{\circ}C$ showed the best surface morphology and crystallinity with nearly no strain. And also this film exhibited outstanding emission characteristics from the viewpoint of full width half maximum of UV emission peak as well as visible emission due to defects. These results indicate that the emission characteristics of the ZnO films are strongly related to their structural characteristics influenced by substrate temperature. Consequently, ZnO films with strong UV emission and nearly no visible emission, which are applicable to UV emission devices, could be grown at the substrate temperature of $600^{\circ}C$ by PLD.
김영환(Y . H . Kim),박구부(G . B . Park),이무하(M . H . Lee),진상근(S . K . Jin) 한국축산학회 1988 한국축산학회지 Vol.30 No.2
The Differential Scanning Calorimetry (DSC) was used to estimate the mixing ratio of various meats $lt;beef round muscle (BR), chicken breast and leg muscle (CB and CL) min fresh pork products. The regression equations of denaturation temperature or enthalpy on mixing ratio was found to be y (denaturation temperature) = 0.51x + 67.30 (r=0.997) or y (enthalpy) = 0.29x + 0.60 (r=0.980) for BR/PT mixing ratio (x). For CL/PT mixing ratio (x) they were y (denaturation temperature) = -1.37x + 68.45 (r= -1.000) or y (enthalpy) = 0.17x + 0.66 (r=0.997). Also, the corresponding lines for CB/PT mixing ratio (x) was y (denaturation temperature) = 4.05x + 66.07 (r=0.966) and y (enthalpy) = -0.09x + 0.69 (r= -0.960). Therefore, the results suggested that analysis of denaturation temperature or enthalpy change in meat mixtures by DSC could be a method for the estimation of mixing ratio between pork and other meats. However, the practical application of this method needs to be studied further.
김영환(Y. H. Kim),이재원(J. W. Lee),박근일(G. I. Park) 한국소성가공학회 2014 한국소성가공학회 학술대회 논문집 Vol.2014 No.10
The wet process shearer has problems such as mass oil waste when the simulated spent fuel rods are sheared by tools, and it has low product ability because of change time of oil and tools. Also, when spent fuel rod is sheared by shearer, round shape is crushed. In order to solve these problems, simulated spent fuel rods(rod-cuts: Zry-4 tube + Al pellet) were manufactured and shearing test was conducted. Also, we selected a method of liquefied nitrogen with a roller blade for a fine circular type, and consisted of shearing module, rotation module and cooling module. We selected a nozzle spray method of cryogenic coolant on the pipe for shearing test. In the results, the problems of wet process shearer such as oil contamination and low product ability were solved by the nozzle spray method of cryogenic coolant. Also, a circular shape of shearing section was maintained by the cutting blades, nozzle splaying and lever. In the case of high speed shearing, tool life was largely affected by tool cooling than pipe cooling, if low speed of shearing, highly effective by it.