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포유자돈 선천성 진전증 증례에서 atypical porcine pestivirus (APPV) 검출
김승채,정창기,윤승민,이기호,양면식,김범석,이승윤,강석진,김원일,Kim, Seung-Chai,Jeong, Chang-Gi,Yoon, Seung-Min,Lee, Ki-Ho,Yang, Myoun-Sik,Kim, Bumseok,Lee, Seung-Yoon,Kang, Seog-Jin,Kim, Won-Il 한국동물위생학회 2017 韓國家畜衛生學會誌 Vol.40 No.3
Congenital tremor (CT) is a sporadic neurodegenerative disease reported in suckling piglets worldwide. Since atypical porcine pestivirus (APPV) was first identified in US in 2015, it has also subsequently detected in Europe and China as a causative pathogen for CT in suckling piglets. Three new-born piglets died from severe tremor was submitted to Chonbuk National University-Veterinary Diagnostic Center (CBNU-VDC) and various tissues (lung, lymph node, brain, intestine) were tested with panpestivirus RT-PCR and APPV NS5B-specific RT-PCR. All of the samples were positive by both of the PCR tests and the partial NS5B sequences of APPV were confirmed by sequencing on the PCR products of APPV NS5B-specific RT-PCR. Therefore, we report the first identification of APPV from a case of CT in suckling piglets in Korea.
돼지 운송 차량과 운전자에 대한 도축장에서의 세척 및 소독 과정에 대한 고찰
김환주 ( Hwan-ju Kim ),김승채 ( Seung-chai Kim ),정창기 ( Chang-gi Jeong ),김재엽 ( Jae-yeob Kim ),김재홍 ( Jae-hong Kim ),백예찬 ( Ye-chan Baek ),정용진 ( Yong-jin Jung ),김원일 ( Won-il Kim ) 한국동물위생학회(구 한국가축위생학회) 2021 韓國家畜衛生學會誌 Vol.44 No.1
Various infectious diseases have threatened swine industries in Korea. One of the major risk factors of disease spread is livestock transport vehicle. For this reason, all abattoirs are required by law to have vehicle washing and disinfection facilities. However, detailed guidelines for cleaning and disinfection procedures were not provided. So in this study, assessment by visual observation was conducted to evaluate how well the cleaning and disinfection procedures of livestock transport vehicles have been performed in four different abattoirs in Jeonbuk, Korea. The observation included length of car washing time, the number of washing bay, type of washperson, type of disinfectant application, length of disinfection time, and compliance of driver’s hygienic procedure. Every vehicle in all abattoirs conducted washing and disinfection procedure. However, there were procedural differences for each abattoirs and defects in the washing and disinfection procedures. In addition to that, the compliance of driver’s hygienic procedure was not sufficient. Hence, detailed manuals on washing and disinfection procedures for involved people are required and it is necessary to establish an inspection and evaluation system.
문보미 ( Bo-mi Moon ),추금숙 ( Keum-sook Chu ),김승채 ( Seung-chai Kim ),김환주 ( Hwan-ju Kim ),김다정 ( Da-jeong Kim ),김원일 ( Won-il Kim ) 한국동물위생학회 2023 韓國家畜衛生學會誌 Vol.46 No.4
This study was carried out to investigate the genotypic diversity of PCV2 and co-infection of PCV3 in the hilar lymph nodes of 700 randomly-selected slaughter pigs. Fourteen samples per each farm were obtained from 50 farms between February and August in 2022. Of the 50 farms, 44 farms that had been positive for PCV2 by RT-PCR were genotyped. As a result of PCV2 genotyping, positive rate of PCV2 DNA was 62.3% (436/700). Among the PCV2 DNA-positive samples, positive rate of a single PCV2 genotype was 79.1% (345/436), while multiple PCV2 genotypes were only detected in 20.9% (91/436). Of the 436 single infection cases, PCV2d genotype was most prevalent. Positive rates of PCV2 and PCV3 were 53.6% and 26.0% at the sample level, 5.1% and 8.0% at the farm level, respectively. And the co-positive rate of two viruses was 8.7% (61/700) at the sample level, 62.0% (31/50) at the farm level. These results demonstrate that PCV2 prevalence in slaughter pigs is very high and co-infection between different PCV2 genotypes and between PCV2 and PCV3 is relatively common. Therefore, genetic diversity and co-infection between other porcine circoviruses should be consistently monitored in the future.