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도축돈의 혈청, 뇨 및 근육에서 sulfamethazine 잔류 조사
추금숙 ( Keum Sook Chu ),오언평 ( Un Pyong Oh ),최인열 ( In Yeol Choi ),송희종 ( Hee Jong Song ),채효석 ( Hyo Seok Chai ) 한국가축위생학회 1997 韓國家畜衛生學會誌 Vol.20 No.2
This study was undertaken to determined the sulfamethazine residues in urine, serum and muscle of slaughtered pigs(n=230) by the method of TLC, EEC-4-plate and HPLC. 1. Inhibition diameter characteristics of reference bacterial substance by EEC-4-plate method and antibacterial inhibition zone was appeared sulfonamides in BS pH 7.2 2. Residual sulfamethazine was detceted from 7 serum(3.04%) and 10 urine(4.35%) by TLC test and concentration of residues was higher in urine than serum. 3. Residual sulfamethazine was detected from 9 muscle(3.91%) by EEC-4-plate method. 4. Positive samples by TLC test and EEC-4-plate method were exceed 0.lppm quantitative analysis from 7 muscle(3.04%).
추금숙 ( Keum Sook Chu ),정우리 ( Woo Ri Jung ),양승혁 ( Seung Hyuk Yang ),이명찬 ( Myung Chan Lee ),손구례 ( Ku Rye Shon ) 한국동물위생학회 2023 韓國家畜衛生學會誌 Vol.46 No.4
The outbreak of lumpy skin disease (LSD), caused by LSD virus (LSDV), in Jeollabuk-do was first confirmed at a Korean cattle farm in Buan-gun on October 24, 2023. Afterwards, thirteen cases (twelve cases in Gochang-gun and a case in Imsil-gun) were further confirmed, resulting in a total of fourteen cases over 25 days until November 17, 2023. Clinical examination were conducted on infected and co-habitting cattle from the LSD-affected farms with particular focus on the presence of nodules throughout the body such as head, neck, chest, femur, head, and perineum. As a results, abnormal clinical signs were observed in fifteen cows: loss of appetite in six cows, high fever in three cows, eye mucosal nodules in a cow, nasal mucosal nodules in six cows, nodules on perineum in five cows, scrotum nodules in two cows, papillary nodules in a cow, and/or skin nodules in eleven cows. By the PCR methods, the common gene of capripox virus and/or the specific gene of LSDV were detected in 35 of the 69 cows tested this study. In the Farm1, capripox virus-specific gene, LSDV wild strain-specific gene, and LSDV vaccine strain-spcific gene were simultaneously detected in affected cows, indicating the cattle farm was affected by various strain of LSDV. As a result of combining clincal examination and PCR test, it was found that clinically and subclinically infeted cows coexist in the LSDV-infected farms. These finding in this study will be a great help in diagnosis and prevention of the LSD in Korean cattle farms.
문보미 ( Bo-mi Moon ),추금숙 ( Keum-sook Chu ),김승채 ( Seung-chai Kim ),김환주 ( Hwan-ju Kim ),김다정 ( Da-jeong Kim ),김원일 ( Won-il Kim ) 한국동물위생학회 2023 韓國家畜衛生學會誌 Vol.46 No.4
This study was carried out to investigate the genotypic diversity of PCV2 and co-infection of PCV3 in the hilar lymph nodes of 700 randomly-selected slaughter pigs. Fourteen samples per each farm were obtained from 50 farms between February and August in 2022. Of the 50 farms, 44 farms that had been positive for PCV2 by RT-PCR were genotyped. As a result of PCV2 genotyping, positive rate of PCV2 DNA was 62.3% (436/700). Among the PCV2 DNA-positive samples, positive rate of a single PCV2 genotype was 79.1% (345/436), while multiple PCV2 genotypes were only detected in 20.9% (91/436). Of the 436 single infection cases, PCV2d genotype was most prevalent. Positive rates of PCV2 and PCV3 were 53.6% and 26.0% at the sample level, 5.1% and 8.0% at the farm level, respectively. And the co-positive rate of two viruses was 8.7% (61/700) at the sample level, 62.0% (31/50) at the farm level. These results demonstrate that PCV2 prevalence in slaughter pigs is very high and co-infection between different PCV2 genotypes and between PCV2 and PCV3 is relatively common. Therefore, genetic diversity and co-infection between other porcine circoviruses should be consistently monitored in the future.