http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
김유아(You Ah Kim),엄영란(Young Ran Um),이정임(Jung Im Lee),김해진(Haejin Kim),임선영(Sun-Young Lim),남택정(Taek Jeong Nam),서영완(Youngwan Seo) 한국생물공학회 2009 KSBB Journal Vol.24 No.6
지질은 생체내에서 여러 가지 중요한 기능을 한다. 하지만 부적절한 식이 섭취로 인한 지방산의 불균형은 성인병을 비롯한 여러 질병의 원인이 된다. 이러한 이유로 지방산에 대한 관심이 높아지고 있으며 특히 어류나 해조류에 다량 포함되어 있는 n-3계 다중불포화지방산과 질병과의 관련성이 밝혀지고 있다. 따라서 본 연구에서는 염생식물이 n-3 계 다중불포화지방산을 함유할 가능성을 확인하기 위하여 26종의 염생식물에 대하여 지방산의 조성과 함량을 조사하였다. 용매 추출된 염생식물 시료는 gas chromatography를 이용하여 분석이 이루어졌으며, 그 결과, 퉁퉁마디, 수송 나물, 염주괴불주머니, 사철쑥, 갯질경 등이 높은 총지방산 함량을 나타냈다. 또한 포화지방산인 palmitic acid (16:0)는 실험 대상 전체에서 나타났을 뿐만 아니라 그 %함량도 대부분의 염생식물에서 가장 높은 것으로 확인되었다. 불포화지방산의 경우, 단일불포화지방산에서는 oleic acid (18:1)가 그리고 n-6계 다중불포화지방산에서는 linoleic acid (18:2)가 높은 %함량을 보였다. 그리고 n-3계 다중 불포화지방산 중에서 천일사초가 26.40%로 LNA의 함량이 가장 높았으며, 뒤이어 모래지치, 수송나물, 갯메꽃 순으로 나타났다. EPA의 함량은 상대적으로 낮은 비중으로 나타났으나 칠면초가 1.89%로 가장 높은 수치를 보였다. DHA는 여러 대상 식물에서 비교적 높은 함량을 보였으며 특히 흰명주아뀌가 14.54%로 높은 함량을 보였다. 이러한 결과는 염생식물이 해조류나 어류의 n-3계 다중불포화지 방산의 함량에 있어서 뒤지지 않음을 보여줄 뿐만 아니라 천연물 화학적이나 식품학적 추가 연구를 통해 여러 질병의 예방과 치료분야에도 기여할 것으로 사료된다. Fatty acid is an important component of many biological processes. However, an imbalance in diet-especially, a n-3 polyunsaturated fatty acids (PUFAs) deficiency-causes several diseases such as diabetes, cardiovascular disease and cancer. In this study, we analyzed the fatty acid contents and compositions of 26 species of Korean salt marsh plants and found high fatty acid contents from S. herbacea (148.75 μg/mg-dry wt.), S. komarvii (119.05 μg/mg-dry wt.), C. heterocarpa (79.23 μg/mg-dry wt.), A. capillaris (71.65 μg/mg-dry wt.), and L. tetragonum (67.02 μg/mg-dry wt.). In the case of saturated fatty acids (SFAs) composition, palmitic acid is richest in most salt marsh plants. On the other hand, oleic acid and linoleic acid are major components of monounsaturated fatty acid and n-6 PUFA, respectively. In addition, n-3 PUFAs such as LNA (linolenic acid), EPA (eicosapentaenoic acid), and DHA (docosahexaenoic acid) known as the main fatty acid components of fish oils and seaweeds, were also found in S. herbacea, S. komarvii, T. tetragonoides, A. capillaris and G. littoralis.
폐 섬유모세포에서 황사의 미세먼지(Particulate Matter 10)가 활성산소족과 TGF-β, NF-κB, PDGF-α, Fibronectin의 생성에 미치는 영향
김아현 ( Ah Hyun Kim ),전수연 ( Su Yeon Chon ),윤진영 ( Jin Young Yoon ),김유진 ( Yu Jin Kim ),경선영 ( Sun Young Kyung ),이상표 ( Sang Pyo Lee ),박정웅 ( Jeong Woong Park ),정성환 ( Sung Hwan Jeong ) 대한결핵 및 호흡기학회 2009 Tuberculosis and Respiratory Diseases Vol.67 No.6
Background: Dust clouds blown by the wind from the arid deserts of Mongolia and Northeast China are known as Asian dust storms. Ambient particulate matter with a diameter <10 μm (PM10) is associated with the exacerbation of respiratory diseases and increased mortality of heart and lung disease patients. The fibrotic effects of PM10 of Asian dust to pulmonary fibroblast cells are unknown. This study examined the production of reactive oxygen species (ROS), TGF-β, NF-κB, PDGF-α and Fibronectin in fibroblasts exposed to Asian dust particles. Methods: Air samples were collected using a high volume air sampler (Sibata model HV500F) with an air flow of 500 L/min for at least 6 hours. The MRC-5 cells were exposed to 0, 50 and 100 μg/mL of PM10 for 24 hours. ROS was detected by measuring the level of oxidized DCF using FACS. TGF-β, NF-κB, PDGF-α and fibronectin were detected by western blotting. Results: There was no increase in the ROS, TGF-β and PDGF-α levels in the MRC-5 cells exposed to PM10. The NF-κB level was higher in the MRC-5 cells exposed to 50 and 100 μg/mL of PM10 for 24 hours. The fibronectin level in the MRC-5 cells after 24 hours incubation with 50 μg/mL PM10 was significantly higher than the control group (PM10 50 μg/mL 113.27±8.65 of control, p=0.005). Conclusion: PM10 from Asian dust increases the activation of NF-κB and fibronectin expression in MRC-5 fibroblast cells.
Graft immaturity and safety concerns in transplanted human kidney organoids
Sun Ah Nam,Eunjeong Seo,Jin Won Kim,Hyung-Wook Kim,Hong Lim Kim,Kyuryung Kim,Tae-Min Kim,주지현,Ivan G. Gomez,Kohei Uchimura,Benjamin D. Humphreys,양철우,Jae Yeon Lee,김진,Dong Woo Cho,Benjamin S. Freedman,Yo 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-
For chronic kidney disease, regeneration of lost nephrons with human kidney organoids derived from induced pluripotent stem (iPS) cells is proposed to be an attractive potential therapeutic option. It remains unclear, however, whether organoids transplanted into kidneys in vivo would be safe or functional. Here, we purified kidney organoids and transplanted them beneath the kidney capsules of immunodeficient mice to test their safety and maturity. Kidney organoid grafts survived for months after transplantation and became vascularized from host mouse endothelial cells. Nephron-like structures in grafts appeared more mature than kidney organoids in vitro, but remained immature compared with the neighboring mouse kidney tissue. Ultrastructural analysis revealed filtration barrier-like structures, capillary lumens, and tubules with brush border in the transplanted kidney organoids, which were more mature than those of the kidney organoids in vitro but not as organized as adult mammalian kidneys. Immaturity was a common feature of three separate differentiation protocols by immunofluorescence analysis and single cell RNA sequencing. Stroma of transplanted kidney organoid grafts were filled with vimentin-positive mesenchymal cells, and chondrogenesis, cystogenesis, and stromal expansion were observed in the long term. Transcription profiles showed that long-term maintenance after kidney organoid transplantation induced transcriptomic reprogramming with prominent suppression of cell-cycle-related genes and upregulation of extracellular matrix organization. Our data suggest that kidney organoids derived from iPS cells may be transplantable but strategies to improve nephron differentiation and purity are required before they can be applied in humans as a therapeutic option.
무슬림 여성의 외상 후 스트레스장애 수준이 건강요인, 불안 및 우울지수, 그리고 심근 자율신경계에 미치는 영향
김만성(Kim, Man-Sung),김성대(Kim, Seong-Dae),김아란(Kim, Ah-Ran),김선휘(Kim, Sun-Hwi),한동유(Han, Dong-Yoo),정일규(Jeong, Il-Gyu) 한국사회체육학회 2016 한국사회체육학회지 Vol.0 No.65
The purpose of this research was to investigate the effects of muslim women’s post traumatic stress disorder(PTSD) level on health, anxiety·depression index, and cardiac autonomic system. The subjects of this study were ninety seven college women(age 21.18±1.70 yrs) in Peshawar, Pakistan. Participants were assigned into four groups according to Posttraumatic stress Diagnosis Scale(PDS): high PTSD level(HP), middle PTSD level(MP), low PTSD level(LP), and no PTSD level(NP). Measurement of anxiety·depression index was conducted by Beck Anxiety Inventory(BAI) and Beck Depression Inventory-Ⅱ(BDI-Ⅱ). For the factors of health status total cholesterol(TC), triglyceride(TC), blood sugar, body fat, hypertension were also measured. The heart rate variability parameters such as low frequency(LFnu), high frequency(HFnu), and total power(TP) were also measured by Pulse-wave HRV analyzer(ubplus T1). In order to find any difference of health-related factors according to PTSD level, one way ANOVA analysis was applied and LSD post-hoc test. Futhermore, Simple regression analysis was applied for subjects in PTSD groups to evaluate the effects of PTSD level on anxiety·depression index and cardiac autonomic system. The results were shown as follows: First, there was no significant difference among four groups in health-related parameters. Second, PTSD level had a significant corelation with depression index(R²=.402, p<.001). Third, PTSD level had a significant corelation with anxiety index(R²=.244, p<.001). Finally, PTSD level had a significant but low corelation with TP index(R²=.103, p<.05). These results suggest that without direct effect of PTSD on physiological factors in young Muslim women, high level of PTSD might induce the negative psychological or emotional response such as depression and anxiety and the changed cardiac autonomic status.
김아현 ( Ah Hyeon Kim ),한선영 ( Sun Young Han ),김형규 ( Hyung Gyoo Kim ),권호근 ( Ho Keun Kwon ),김백일 ( Baek Il Kim ) 대한예방치과·구강보건학회 2010 大韓口腔保健學會誌 Vol.34 No.2
Objectives. This study examined the characteristics of a high caries risk group of 12-year-old children in Korea. Methods. The oral health status and interview data were collected from 1,755 children (888 males, 867 females), aged 12 years, who participated in the Korea National Oral Health Survey in 2006. The DMFT values of the subjects were sorted. The upper one third was selected as the high risk caries group (N=585) and the other two thirds were classified as the low risk caries group (N=1,170). This study used the demographic and social variables, such as gender, area of residence and governmental support for lunch in school. The variables for the oral health status, oral health related-consciousness and behavior were the number of dental sealants of the first molar, self-perceived oral health, tooth brushing after lunch, average frequencies of tooth brushing per day and the mean frequency of daily snack consumption. The associations between the demographic and social variables, oral health status, oral health related-consciousness and behavior and high risk group were evaluated by logistic regression analysis. Results. Most of the high caries risk group had less than 2 sealant teeth on the first molar (94.2%) and lived mainly in metropolitan areas (46.8%, p<0.05). They had a poorer self-perceived oral health (41.8%) than the low risk group and more 2∼3 times the snack intake per day (p<0.05). The determinants of the high risk group were whether the first molar was sealed (‘under 2’, OR=6.05), self-perceived oral health (‘good’, OR=1.77, ‘poor’, OR=2.67), mean frequency of daily snack intake (‘2∼3 time’, OR=1.31, ‘more than 4’, OR=2.34) and area of residence (‘city’, OR=0.79). Conclusions. The characteristics of the high caries risk group in Korean 12-year-old children were less than 2 sealant teeth on the first molar, poor self-perceived oral health, high snack intake per day and living in metropolitan areas.
아로니아, 비트, 백년초 분말 첨가 순무 물김치의 품질특성 및 항산화 활성 비교
김선회,김지형,엄선아,한영선,허명제,Kim, Sun Hoi,Kim, Ji Hyeung,Eom, Sun Ah,Han, Young Sun,Heo, Myung Je 한국식품조리과학회 2018 한국식품조리과학회지 Vol.34 No.3
Purpose: This study examined the effects of aronia, beet and prickly pear powder on the properties of mul-kimchi made of turnip. Methods: Each turnip mul-kimchi was made by adding 0.2% aronia, beet or prickly pear powder. After a 24 hour fermentation process at $25^{\circ}C$, it was stored at $4^{\circ}C$. The antioxidant activities and quality characteristics of turnip mul-kimchi during 34 days of storage at $4^{\circ}C$ were compared. The antioxidant activities were evaluated using DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) and ABTS (2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging methods, as well as FRAP (ferric reducing antioxidant power) assays and the total polyphenol content. Results: The antioxidant activity of aronia-added turnip mul-kimchi was higher than that of the control and others. The vitamin C content was the highest on day 6, with the following levels: aronia-added turnip mul-kimchi (182.36 mg/kg), beet-added turnip mul-kimchi (171.80 mg/kg), prickly pear-added turnip mul-kimchi (163.80 mg/kg) and control group (158.85 mg/kg). Conclusion: The addition of aronia powder can improve the quality characteristics and antioxidant activities of turnip mul-kimchi.
천선아(Sun Ah Chun),최설민(Sul Min Choi),김대영(Dae Young Kim),박현선(Hyun Sun Park),이미경(Mi Kyung Lee),우동준(Dong Jun Woo),배혜진(Hye Jin Pae),신동훈(Dong Hun Shin),김형식(Hyung Sik Kim),안미영(Mi Young Ahn),곽승준(Seung Jun Kwac 한국독성학회 1999 Toxicological Research Vol.15 No.3
Eye irritation of Sangmosu was evaluated in New Zealand White rabbits. In ocular irritation test, any injury on iris, conjunctival membrane, and cornea was not observed. No injuries of the ocular mucous membrane were also recorded. These results indicate that Sangmosu was not considered to be irritant in test organs of animals.
Pseudomonas sp. PY002에서 Exotoxin A의 생성에 미치는 철 이온의 영향과 Exotoxin A 유전자의 클로닝
최선아,김호상,최지영,강정숙,김춘성,김덕례,김영주,여명구,박열,Choi, Sun-Ah,Kim, Ho-Sang,Choi, Ji-Young,Kang, Jeong-Suk,Kim, Chun-Sung,Kim, Duck-Lae,Kim, Young-Ju,Yeo, Myeong-Gu,Park, Yeol 한국미생물학회 1999 미생물학회지 Vol.35 No.1
Pseudomons sp. PY002의 exotoxin A 의 발현 양상을 관찰하기 위하여 P.aeruginosa PAO1의 anti-exotoxin A와 immunoblot hybridization을 실시한 결과 배지내에 유용 가능한 철이 고갈됨에 따라 exotoxin A의 발현양은 점차적으로 증가하는 양상을 보였으며, CAS 배지에 점적한 배양 상층액에서 siderophore의 발현양도 증가함을 보였다. P.sp.PY002 의 genomix library를 제조하여, exotoxin A를 분비하는 2개의 클론을 선별하려 pETA23과 pETA42 로 명명한 후, 반응성이 강한 Peta42를 선발하였다. pETA 42는 약 1.7kb 크기의 insert를 가지며, 양쪽 말단에 cloning site 인 pstI site 가 존재하며 2개의 NcoI, 1개의 PvuII, 1개의 SstI , 3개의 SmaI, 1개의 KpnI, 3개의 HaeII, 1개의 EcoRI site가 존재하였다. By SDS-polyacrylamide gel elcctrophoresis (SDS-PAGE) and immunoblot analysis that a protein with 66,000 daltons in size was recognized by P. aeruginosa anti-exotoxin A from P. sp. PY002. The yields of exotoxin A in P. sp. PY002 culture were influenced by the concentration of iron in the culture media. Increasing of the exotoxin A production and siderophore production was made slight increasing in the MKB medium. On the other hand, to clone the gene encoding the exoloxin A genomic library of P. sp. PY002 was constructed in pBluescript SK(+). From this library a exotoxin A homologous gene was isolated by immunological hybridization method using P. aemginosa anti-exoloxin A as a probe. Two putative clones were isolated and designated pETA23 and pETA42. Thc restriction analysis ol pETA42 demonstrated that thc 1760 bp insert contained one NcoI, PvuII, SstI, Kpnl and EcoRI site and three SmaI and HaeD sites.