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난소의 장액성 낭선암 환자의 암조직, 낭종액 및 혈청의 Amylase에 관한연구
조수현,고재경,김두상,김세덕 한양대학교 의과대학 1989 한양의대 학술지 Vol.9 No.1
In order to investigate whether serous ovarian neoplastic amylase (SONA) could be used as a diagnostic or biochemical marker for serous cystadenocarcinoma of ovary, amylase activity was determined in the cystic fluid, serum and ovarian cancer tissue of patients with ovarian serous cystadenocarcinoma. Also studied was the substrate specificity of the enzyme separated and purified from cystic fluid of serous cystadenocarcinoma by a phosphocellulose and DEAE-cellulose column chromatographies. 1. Amylase activity in the cystic fluid, serum and ovarian cancer tissues in patients with ovarian serous cystadenocarcinoma was significantly increased and the positive rate of the enzyme activity as a marker for serous cystadenocarcinoma was observed to be 100% in the cystic fluid, 60% in serum and 40% in the ovarian cancer tissue. 2. Amylase in the cystic fluid of ovarian serous cystadenocarcinoma was separated by a phosphocellulose column chromatography into two peaks (peak Ⅰ and Ⅱ) and one major peak (peak Ⅰ) amylase of the two peaks was further separated by a DEAE-cellulose column chromatography into three peaks(peak Ⅰ-1, Ⅰ-2 and Ⅰ-3). 3. The substrate specificity of peak Ⅰ-1, Ⅰ-2 and Ⅰ-3 amylases appeared to be similar toward starch and dextrin, but different with each other against glycogen and amylopectin. 4. The PAGE patterns for peak Ⅰ-2 protein was shown to be slightly different from that for peak Ⅰ-3 protein. These results suggested that activities of amylase in the cystic fluid, serum and ovarian cancer tissues could be used as a diagnostic or biochemical marker for serous cystadenocarcinoma of the ovary and that three peak (peak Ⅰ-1, Ⅰ-2 and Ⅰ-3) amylases isolated from the cystic fluid of serous cystadenocarcinoma might be isozymes with different properties.
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Use of Array Detector for Real-time Spectoscopic Ellipsometer
안일신,김세덕 한양대학교 이학기술연구소 1999 이학기술연구지 Vol.1 No.-
고속분광 엘립소미터를 위해 photodiode array 검출기를 이용하였다. 이때 각 부품의 시간적 요구사항을 만족시키기 위하여 array 구동용 전자회로를 직접 설계 제작하였는데 512의 채널을 읽어 내는데 필요한 시간인 read-out time이 편광기의 회전 속도를 제한하였다. 하지만 광학 주기당 3-4회의 부분 적분법을 적용함으로써 한 쌍의 엘립소미터 스펙트럼을 측정하는데 20 m초 이내의 측정속도를 실현할 수 있었다. 각 채널로부터 일정한 반응값을 얻을 수 있었으며 연속측정에서도 평균정도에 따라 0.04에서 0.007도 정도의 정밀도를 얻었다. We employed photodiode array as a detection system for fast scanning spectroscopic ellipaometer. Controller for data acquisition was designed to accommodate all timing requirements. Pixel read-out time for 512 channels limited the rotational frequency of polarizer. However, when we employed partial-integration method to take 3 or 4 spectra per optical cycles, we could achieve the fastest data acquisition speed of less than 20 ms for a pair of ellipsometric spectra. We obtained quite uniform response from ll channels and the repetitive measurements showed 0.04-0.007 degree of precision depending on averaging.
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분광 엘립소메트리의 응용을 위한 0차 Retardation Plate의 Alignment
이용달,박명규,김세덕,방경윤,안일신 한양대학교 이학기술연구소 2000 이학기술연구지 Vol.2 No.
A zero-order retarder was fabricated using two pieces of magnesium fluoride plates. In order to achieve desired zero-order retardation for specific wavelength, both the thickness of plates and the alignment of two fast axes were very important. We found that the alignment should be checked using broad band detection system for spectroscopic application, We devised alignment method by which the two fast axes lie perpendicularly to each other. The periodic oscillation feature in retardation angle versus wavelength disappeared with good alignment. We also developed calibration method to deduce the retardation angle over a wider spectral range using rotating compensator ellipsometer. 두 조각의 마그네슘 플로라이드 판을 이용한 0차 retarder를 제작하였다. 특정한 파장에 대하여 원하는 0차 retardation을 얻기 위해서는 판의 두께와 alignment가 매우 중요하였다. 분광학적인 응용을 위해서는 넓은 파장 영역을 측정할 수 있는 detection system의 사용이 필요하였다. 이를 이용하여 두 판의 fast axes가 서로 수직으로 놓이게 할 수 있는 alignment 방법을 개발하였다. 파장에 대한 retardation 각도에서의 주기적인 진동의 모양은 alignment를 잘하게 되면 사라지게 된다. 또한 rotating compensator ellipsometer를 사용 할 때 넓은 분광영역에 걸친 retardation angle을 측정해 낼 수 있는 calibration 방법을 개발하였다.
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임신기간에 따른 혈청 Protein-bound Carbohydrate량과 α1 Glycoprotein의 분획 백분률에관한 고찰
양행식(HS Yang),양준영(JY Yang),김세덕(SD Kim) 대한산부인과학회 1980 Obstetrics & Gynecology Science Vol.23 No.9
임신 기간에 따른 PBC량 및 1-GP분획 백분율의 변동을 밝히기 위하여 1973년 11월 1일부터 1978년 9월 30일까지 중앙대학교 의과대학 부속 성심병원 산부인과에서 임신 반응검사와 내진 등으로 임신임이 확진된 299명에 대하여 phenol-sulfuric method로 PBC량을 정량하고 전기영동을 하여 periodi-acid-Schiff염색을 한 후 1-GP분획 백분율을 얻어 그 무월경 주수 별로 관찰한 결과 다음과 같은 결론을 얻었다. 1. 임신시 PBC량은 증가는 되었으나 임신 기간과는 특이한 상관관계가 없었다(r=0.0003, F<0.5). 2. 1-GP의 분획 백분율은 임신시 증가는 되었으나 임신기간과는 특이한 상관관계가 없었다(r=0.002a, F<0.5). It was already confirmed that there was some biochemical and immunologic changes in the pregnancy and was also reported that the serum level of pregnancy-associated plasma protein, which is a kind of protein-carbohydrate complexes, were increased in the pregnancy and disappeared from the serum 6 weeks after delivery. Also, it was reported that the serum levels of the protein-bound-carbohydrate were increased in the patients with diabetes mellitus, myocardial infraction and pregnancy, and some components, which were isolated by electrophoresis, were increased in the patients with diabetes mellitus, pregnancy and malignancies. In order to observe the correlative changes of serum levels of protein-bound-carbohydrate and percentage of alpha-1 glycoprotein in the pregnancy, the author collected sera from 299 pregnant women, who were confirmed with pelvic examination and planosec test in the Dept. of OB & GY, Medical School, Chung Ang University from Nov., 1977 to Sept., 1978. And calculated the serum levels of protein-bound-carbohydrate by phenolsulfuric method, and percentage of alpha-1 glycoprotein by electrophoresis with zip zone electrophoresis kit(Helena Co., USA), periodic-acid-Schiff`s staining and densitometer(Quick scan Jr., Helena Co., USA) and observed the correlation between the data and duration of pregnancy. The following results were obtained; 1. The serum levels of the protein-bound-carbohydrate were not correlated with duration of pregnancy(r=0.0003, F<0.5). 2. The percentage of serum alpha-1 glycoprotein were not correlated with duration of pregnancy(r=0.0022, F<0.5).
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