Differential Promoter Methylation and Histone Modification Contribute to the Brain Specific Expression of the Mouse Mbu-1 Gene

김병탁,김선정,Seongeun Kang 한국분자세포생물학회 2012 Molecules and cells Vol.34 No.5

Mbu-1 (Csrnp-3) is a mouse gene that was identified in our previous study as showing highly restricted expres-sion to the central nervous system. In this study, to eluci-date the regulatory mechanism for tissue specificity of the gene, epigenetic approaches that identify the profiles of CpG methylation, as well as histone modifications at the promoter region were conducted. Methylation-specific PCR revealed that the CpG sites in brain tissues from embryo to adult stages showed virtually no methylation (0.052- 0.67%). Lung (9.0%) and pancreas (3.0%) also showed lower levels. Other tissues such as liver, kidney, and heart showed much higher methylation levels ranging from approximately 39-93%. Treatment of 5-aza-2-deoxycytidine (5-Aza-dC) significantly decreased promoter methylation, reactivating Mbu-1 expression in NG108-15 and Neuro-2a neuronal cells. Chromatin immunoprecipitation assay revealed that 5-Aza-dC decreased levels of acetylated H3K9 and methylated H3K4, and increased methylated H3K9. This result indicates that CpG methylation converses with histone modifications in an opposing sense of regulating Mbu-1 expression.

限界農地 遊休化 要因과 活用方案 : 西部 慶南의 事例를 中心으로

金秉鐸,李榮萬,金義庚 경상대학교 지역개발연구소 1995 地域開發硏究所 資料集 Vol.1995 No.1

針刺戟한 흰쥐의 腦抽出物이 疼痛역値에 미치는 影響

김병탁 大田大學校 1989 論文集 Vol.8 No.1

In order to investigate analgesic effect of acupuncture and its action mechanism, experimental rats were noodlod at Tsusanli (S36) for 3 minatos. The animals were then killed by dacapitation and the brains were extracted with acetone. The brain extract dissolved in saline so that 0.5ml extract was equivalent to 1/2 brain, and 0.5ml of the brain extract was administered, s.c. to the rat for the test. The analgesic effect of the brain extract was assessed by the analgesy-meter at 20 minute intervals for 60 minutes. The results are as followings. 1. In the group of injection with brain extract of non-acupunctured rats, the pain threshold did not change as compared with control. 2. In the group of injection with brain extract of acupunctured rats, the pain threshold was increased as compared with control, showed a significant increase at 40 minutes after injection with brain extract. According to above results, these evperimental observations suggest that some chemical compunds are produced during acupuncture stimulation. The present study shows that acupuncture may serve to stimulate the release of endogenous morphine-like substances, namely Endorphins, which in turn mediate the analgesic effect.

農業人力 育成事業의 展開過程과 發展方案

金秉鐸,金厚根 慶尙大學校 地域開發硏究所 1999 地域開發硏究 - 慶尙大學校 地域開發硏究所 Vol.10 No.-

열 및 기계적 하중을 고려한 실린더 블럭의 응력 해석

金秉倬 釜山工業大學校 1994 論文集 Vol.36 No.-

In this study, the thermal and the mechanical behavior characteristics of the cylinder block of a small 3-cylinder, 4-stroke gasoline engine were analyzed using the 3-dimensional finite element method. In the analysis of thermal behaviors, the heat transfer characteristics based on the experimental data and thermal stress due to the thermal loading were investigated. And then mechanical stress analysis was carried out at a time later than the clarification of the path along which mechanical loads are transmitted to the main bearing caps in order to examine the stress and deformation characteristics form a point of view concerning their effects on the engine operation. The calculated thermal stress and the mechanical stress were compared each other with respect to their levels and the tendencies of their distributions. And then with the superposition of their stress components, the resultant total stress and the deformed shape were obtained.

발간사

김병탁 대전대학교 한의학연구소 1995 혜화의학회지 Vol.3 No.2

N/A

정압 스러스트 베어링의 유체-구조물 사이의 상호작용에 관한 연구

김병탁 한국기계가공학회 2006 한국기계가공학회지 Vol.5 No.3

In this study, the behavior characteristics of a hydrostatic thrust bearing used in hydraulic equipment was analyzed using a commercial finite element program, ADINA. The solid domain was modeled with the fluid domain simultaneously to solve the fully coupled problem, because this is a problem where a fully coupled analysis is needed in order to model the fluid-structure interaction(FSI). The results such as bearing deformation, stress, film thickness and lifting bearing force were obtained through FSI analysis, and then they were compared with the results calculated from the classical method, a single step sequential analysis. It was found that the result difference between two analyses was increased according to the injection pressure. Therefore, in case of high pressure loading, it is desirable to conduct the FSI analysis to examine the deformation characteristics of a hydrostatic slipper bearing.

Swaging시 P/S 호스의 변형 특성에 대한 유한요소해석 ( Finite Element Analysis for the Deformation Characteristics of a P/S Hose in the Swaging Process )

김병탁,김형제,송한종,강창기 대한기계학회 2001 대한기계학회 춘추학술대회 Vol.1 No.1

사질토 지반에서 단일 강성말뚝의 수평거동에 대한 시공방법의 영향

김병탁,김영수,서인식 한국지반공학회 1999 한국지반공학회논문집 Vol.15 No.6

본 논문은 균질 및 비균질의 사질토 지반에서 항타 시공된 단일 강성말뚝의 수평거동에 대한 모형실험 결과들을 고찰하였다. 본 연구의 목적은 말뚝의 수평거동 특성에 대한 말뚝 시공상태 (Driven & Embedded), 말뚝 근입길이에 대한 하부지반의 두께비 (H/L),그리고 지반의 상대밀도의 영향에 관하여 실험적인 연구를 수행하고 이러한 영향들을 정량화 할 수 있는 실험결과를 얻었다. 모형 실험 결과들에 의하면, 수평거동은 느슨한 균질지반에서 시공방법에 상당히 의존하는 것으로 나타났다. 말뚝시공시 항타방법에 의존할 경우 균질지반에서는 느슨한 지반일수록 그리고 비균질 지반 $(E_{h1}/E_{h2}/=0.18)$에서는 상부층 두께가 클수록 수평변위 감소에 상당한 효과를 얻을 수 있으나, 최대 휨모멘트 감소는 수평변위 감소와 정반대의 결과를 나타냈다. 수평변위 측면에서, 매입말뚝에 대한 항타말뚝의 변위비 $(y_{Driven}y_{Embedded})$는 균질지반의 각 상대 밀도에 대하여 0.65-0.88 $(D_r=90%)$와 0.38-0.65 (D$_{r}$=61.8%)의 범위로 그리고 비균질지반에서 0.6-0.88 의 범위로 나타났다. 또한, 본 연구에서는 $y_D/y_E \;와\; NBM_D/MBM_E$에 대한 항타고와 H/L의 영향들을 모형실험 결과들로부터 실험식으로 제안하였다. This paper shows the results of model tests on the lateral behavior of single rigid pile, which was constructed by driving, in homogeneous and non-homogeneous (two layered) NakDong River sands. The purpose of the present paper is to investigate the effect of ratio of lower layer thickness to embedded pile length, relative density of sand and pile construction conditions (Driven & Embedded piles) on the characteristics of lateral behavior of single pile. These effects can be quantified only by the results of model tests. As a model result, the lateral behavior depends upon the pile construction condition in loose-density soil more than in high-density soil. If the pile construction depends upon driving construction, the decrease of deflection remarkably increases for both loose homogeneous sand and non-homogeneous soil$(E_{h1}/E_{h2}/=0.18)$ with high thickness of upper layer but the decrease of maximum bending moment shows the opposite result to the decrease of deflection. And, with respect to deflection, it was found that the deflection ratio $(y_{Driven}y_{Embedded})$ of embedded to driven piles has the ranges of 0.65 - 0.88 $(D_r=90%)$0.38 - 0.65 $(D_r=61.8%)$ for each relative density of homogeneous soil and the range of 0.6 - 0.88 for non-homogeneous soil. Also, in this study, the experimental equation for the effects of drop height (DH) and H/L on the ratios of $y_D/y_E\; and MBM_D/MBM_ E$ is suggested from model tests.

2개층 사질토지반에서 정방형 기초의 지지력 특성

김병탁,김영수,이종현 한국지반공학회 2001 한국지반공학회논문집 Vol.17 No.4

본 연구는 균질 및 2개층 비균질지반에서 사질토지반 상에 놓인 정방형 기초의 극한지지력과 침하에 대하여 고찰하였다. 본 연구는 얕은기초의 거동에 대한 정방형 기초의 크기, 지반 상대밀도, 기초 폭에 대한 상부층의 두께 비(H/B), 상부층 아래 경계면의 경사($\theta$) 그리고 지반강성비의 영향을 규명하기 위하여 모형실험을 수행하였다. 동일 상대밀도에서 지지력 계수($N_{{\gamma}}$)는 일정하지 않으며 기초 폭에 직접적으로 관련되며 지지력계수는 기초 폭이 증가함에 따라 감소하였다. 기초크기의 영향과 구속압력의 영향을 고려하는 Ueno 방법에 의한 극한지지력의 예측값은 고전적인 지지력 산정식보다 더 잘 일치하며 그 값은 실험값의 65% 이상으로 나타났다. $\theta$=$0^{\circ}$인 2개층 지반의 결과에 근거하여, 극한지지력에 대한 하부층 지반의 영향을 무시할 수 있는 한계 상부층 두께는 기초 폭의 2배로 결정되었다. 그러나, 73%의 상부층 상대밀도인 경우는 침하비($\delta$B) 0.05 이하에서만 이 결과가 유효하였다. 경계면이 경사진 2개층 지반의 결과에 근거하여, 상부층의 상대밀도가 느슨할수록 그리고 상부층의 두께가 클수록 극한지지력에 대한 경계면 경사의 영향은 크지 않는 것으로 나타났다. 경계면의 경사가 증가함에 따른 극한침하량의 변화는 경계면이 수평인 경우($\theta$=$0^{\circ}$)를 기준으로 0.82~1.2(상부층 $D_{r}$=73%인 경우) 그리고 0.9~1.07(상부층 $D_{r}$=50%인 경우) 정도로 나타났다.Markup Language 문서로부터 무선 마크업 언어 문서로 자동 변환된 텍스트를 인코딩하는 경우와 같이 특정한 응용 분야에서는 일반 문자열에 대한 확장 인코딩 기법을 적용할 필요가 있을 수 있다.mical etch-stop method for the etching of Si in TMAH:IPA;pyrazine solutions provides a powerful and versatile alternative process for fabricating high-yield Si micro-membranes. the RSC circle, but also to the logistics system in the SLC circle. Thus, the RSLC model can maximize combat synergy effects by integrating the RSC and the SLC. With a similar logic, this paper develops "A Revised System of Systems with Logistics (RSSL)" which combines "A New system of Systems" and logistics. These tow models proposed here help explain several issues such as logistics environment in future warfare, MOE(Measure of Effectiveness( on logistics performance, and COA(Course of Actions) for decreasing mass and increasing velocity. In particular, velocity in logistics is emphasized.