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톱밥재배용 표고 품종의 단핵균사체와 2핵균사체의 한천과 톱밥배지에서의 균사생장
김민경,김성태,김소라,김은지,진미경,이용국,서건식,Kim, M.K.,Kim, S.T.,Kim, D.Y.,Kim, E.J.,Jin, M.K.,Lee, Y.K.,Seo, G.S. 국립한국농수산대학교 교육개발센터 2017 현장농업연구지 = Journal of practical agricultural resear Vol.19 No.1
표고 육종 소재를 개발하기 위하여 톱밥봉지재배용 품종의 2핵균사체와 단핵균사체를 한천배지와 톱밥배지에서 배양적 특성을 조사하였다. 품종은 산조 701호, 산조 705호, 참아람, 추제 2호를 사용하였다. 공시 표고 품종의 균사생장은 산조 705호를 제외한 품종 모두 PDA에서 가장 양호하였으나 산조 705호는 MCM에서의 균사 생장이 가장 양호하였다. 품종에 관계없이 표고 균사는 MEA에서 가장 안정적인 균사생장을 보였다. 톱밥배지에서 공시균주의 균사생장은 품종 간의 큰 차이는 보이지 않았으나 산조 701호와 705호보다 약 10%정도 빠르게 생장하는 것으로 나타났다. 산조701호와 참아람의 단핵균사체는 2핵 균사체보다 균사생장이 저조하였다. 2핵균사체는 참아람이 산조 701호보다 균사생장이 양호하였으나 단핵균사체는 전체적으로는 산조 701호가 참아람보다 균사생장이 양호하였다. 선발된 단핵 균사체는 균사생장이 매우 다양하고, 형성된 균총의 형태도 매우 다양하여 유전적으로 매우 다양한 단핵 균사체가 선발된 것으로 추정할 수 있고, 이들 단핵 균사체는 교배육종을 위한 좋은 육종 소재로 기대된다.
LED 광원이 느티만가닥버섯 균의 균사 생장과 자실체 생육에 미치는 영향
김민경,이용국,서건식,Kim, M.K.,Lee, Y.K.,Seo, G.S. 국립한국농수산대학교 교육개발센터 2020 현장농업연구지 = Journal of practical agricultural resear Vol.22 No.1
LED 광원이 느티만가닥버섯 재배에 미치는 영향을 알기 위하여 6종의 파장이 다른 NUV(wave length 405nm), blue(wave length 460nm), green(wave length 530nm), yellow(wave length 590 nm), red(wave length 630nm), white(wave length 6500K) 광을 조사하면서 균사 생장 및 자실체 생육과 수량을 조사하였다. 1. 느티만가닥버섯균의 균사 생장에는 광이 필요하지 않지만, red 광 처리구에서 암조건과 비슷한 균사 생장을 보였다. 그러나 blue, green, yellow 광 처리구에서는 암배양보다 약 30~40%의 균사 생장 저해를 보였다. 2. LED 광이 느티만가닥버섯의 자실체 생육에 미치는 영향을 조사한 결과, 암조건에서는 자실체 발달이 안 되는 경우도 있으므로, 느티만가닥버섯의 자실체 생육에는 광 조사가 꼭 필요하다. 단파장인 blue와 green광, 복합광인 white을 조사한 처리구에서 자실체 갓과 대 발달이 안정적이면서 수량이 우수하였다. 장파장인 red 와 yellow 광 처리구에서는 갓 발달과 색택이 불량하고, 대는 길고 꼬임 등의 기형을 보였다. 3. 단일광 조사만으로는 우수한 품질의 버섯을 안정적으로 생산하기 어렵고, 각 생육 단계마다 효과가 있는 광을 조사하는 것도 어렵다. blue와 green, white, red 광을 조합하여 생육 기간 동안 혼합광을 조사하여 갓과 대의 균일성 및 갓 색택, 수량을 분석한 결과, 가장 이상적인 LED 광 조합은 blue와 white 광이었다. A edible mushroom, Hypsizygus marmoreus is commercially cultivated. However, the researches of cultivation and physiological characteristics were not conducted in Korea. In this study, we conducted on artificial cultivation of H. marmoreus and elucidated the effect of light on the mycelial growth and fruit body development using LED light sources with different wavelength; blue (peak wave length 460nm), green(peak wave length 530nm), yellow(peak wave length 590nm), red(peak wave length 630nm), and white as positive control. Mycelial growth of H. marmoreus strains were inhibited about 30~40% in inhibition ratio under the illumination with blue, green, yellow LED light. However, red LED light was not inhibited. Elongation of stipe was effective under the long wave length such as yellow and red light. Abnormal fruit body was produced under the long wavelength and dark. However, development of pileus was effective under the short wavelength such as green and blue light. Also, as a result of cultivation with mixed light for high quality and harvest, many effective numbers and yields of fruiting bodies were obtained in the mixed treatment of blue and white light, and pileus developed well.
김민경,김동욱,강희완,서건식,Kim, M.K.,Kim, D.U.,Kang, H.W.,Seo, G.S. 국립한국농수산대학교 교육개발센터 2018 현장농업연구지 = Journal of practical agricultural resear Vol.20 No.1
A edible mushroom, Clitocybe maxima (Lentinus giganteusis) commercially cultivated in China and Taiwan. However, the researches of cultivation and cultural characteristics were not reported in Korea. In this study, we conducted on cultural characteristics and artificial cultivation of C. maxima. Six isolates were collected from China(3 isolates, commercial strain), Taiwan(1 isolate, commercial strain) and Korea(2 isolates, wild type). C. maxima and L. giganteus collected in China and Taiwan, respectively, are the same in China and are estimated to be of the same species as cultured characteristics. The mycelial growth of the collected strains was not significantly different in agar medium but it showed the best growth in YPMG in liquid culture. Optimum temperature for mycelial growth and induction of fruit body were 25℃ and 30℃, respectively. In order to artificial cultivation of C. maxima, cultural characteristics and artificial cultivation were carried out using agricultural by-products and forestry by-products materials. Mycelial growth was suitable in rice straw, cottonwood sawdust, corncob and rice seed medium, and it was selected as a cultivation medium. The suitable medium for artificial cultivation of C. maxima was selected to mixed medium 2(compounding ratio(v/v): 55% of hardwood sawdust, 5% of cottonseed pellets, 10% of cottonseed, 15% of beet pulp, 15% of swollen rice husks). It took about 30 days to be able to harvest, it was faster than oyster mushrooms. The cultivation period was about 30days. A isolate, CMA-002 was not initiation to fruit body primordiuma on the used cultivation substrate. Other 5 isolates were initiate and development to fruit body on the substrate used in this study. The strain CMA-003 was initiated to be fruiting body by 8~10 days after induction of fruiting body in all of the substrates. Isolate CMA-003 was generate to a bundle fruit body. Other isolates, however, were form fruit body individually. The CMA-003 strain was likely highly recommendable strains for farming. The optimum conditions for the induction and growth of C. maxima fruit body were 25~30℃, 8 hr illumination per day with white fluorescent lamp, 90~95% relative humidity, and 1,500 ppm of CO<sub>2</sub> concentration in a cultivation room.
김민경,이용국,서건식,Kim, M.K.,Lee, Y.K.,Seo, G.S. 국립한국농수산대학교 교육개발센터 2019 현장농업연구지 = Journal of practical agricultural resear Vol.21 No.1
King oyster mushroom(Pleurotus eryngii) is one of the most commercially important mushrooms in Korea. Development of fruit body and disease occurrence are sensitive to environmental conditions such as temperature, humidity, carbon dioxide(CO<sub>2</sub>) concentration. The purpose of this study was to investigate the changes in the growth environment of king oyster mushroom by installing Airocide, an air purifier for the purpose of improving mushroom cultivation environment. The results of the environment conditions, identification of pathogenic organisms and pathogenesis during the cultivation were as follows. Airocide operation increased the CO<sub>2</sub> concentration of the cultivation room by more than 400 ppm on average, but the increase of CO<sub>2</sub> concentration at this level had little effect on the quality and growth of fruit body. Operation of the Airocide tended to reduce the air humidity of the cultivation room and required more humidification. In humidifying conditions, the Airocide has the effect of lowering the species and density of bacteria and reducing bacterial symptoms and abnormal fruiting body of mushroom. Pseudomonas sp., the mushroom pathogen, was isolated from the cultivation room without Airocide, resulting in serious disease and loss of yields, so that only about 83% of substrate could harvest normal fruiting bodies. No disease symptom caused by bacteria and fungi in the cultivation room with Airocide. Trichoderma sp., Penicillium sp. and Cladosporium sp. were isolated from all experimental conditions, but did not inhibit fruit growth or caused diseased.
FDM기술 에서 원형단면 필라멘트 재료의 노즐 유입속도에 따른 압출량
김민경(M. K. Kim),최용선(Y. S. Choi),이인환(I. H. Lee),김호찬(H. C. Kim),조해용(H. Y. Cho),김범근(B. K. Kim) 대한기계학회 2015 대한기계학회 춘추학술대회 Vol.2015 No.11
The additive manufacturing technology(AM) has been used for a variety of prototyping. Recently, the study of a variety of additive manufacturing technology has been conducting to fabricate the actual usable product. The most commonly used techniques are FDM technology on the additive manufacturing. FDM technique has the advantage that the material and equipment is low. However, the surface roughness of an FDM structure has a disadvantage not superior to other additive manufacturing technology. In this study, We configured the FDM system for perform study to improve the surface roughness of the fabricated structure using FDM technology. Also, We were quantified the Filament material extrusion amount of FDM system through the experiment.
오일프리 주공기 압축기 진동 및 소음관련 최적화 방안 연구
김민경(M. K. Kim),김철수(C. S. Kim),안찬회(C. H. An),김승곤(S. G. Kim),한재진(J. J. Han),정승섭(S. S. Jung),조용성(S. Y. C),양성화(S. H. Yang) 대한기계학회 2017 대한기계학회 춘추학술대회 Vol.2017 No.11
주행성능과 승차감에 영향을 미치는 진동 및 소음 영향인자를 차량운용자 관점에서 고찰하고자 한다. 기존차량과 신조차량시 설계되는 부분이 다른 만큼 각각의 운용적인 조건을 개발 과정에서 수렴하여 설계시 최적화를 고려하는 것이 필요하다. 본 연구에서는 기존 차량운행시 설계사양에서 제시된 사양과 향후 부품교체에 따라 신규로 적용될 부품간 운용자, 고객 입장을 고려하기 위하여 영향인자를 분석 제안하였다. The vibration and noise influencing factors affecting driving performance and ride comfort are examined from the viewpoint of vehicle operator. It is necessary to consider the optimization in the design by converging the operational conditions of each existing vehicle and the new vehicle as different parts are designed. In this study, we analyzed the influential factors to consider the new operator`s position between the part and the customer according to the specifications given in the design specifications and the parts replacement in the existing vehicle.