Pseudomonas aeruginosa KS47에 의한 절삭유의 생물학적 분해

김란희,이상섭,Kim, Lan-Hee,Lee, Sang-Seob 한국미생물학회 2008 미생물학회지 Vol.44 No.1

Cutting oils are emulsionable fluids widely used in metal working processes. Their composition is mineral oil, water, and additives (fatty acids, surfactants, biocides, etc.) generating a toxic waste after a long use. Cutting oils also affect colour, taste and odour of water, making it undesirable for domestic and industrial uses. In these days, conventional treatment methods as evaporation, membrane separation or chemical separation have major disadvantages since they generate a concentrated stream that is more harmful than the original waste. In this study, our purpose is to reduce cutting oils by using biological treatment. Eighty one strains were isolated from cutting waste oil of industrial waste water sludge under aerobic conditions. Among these strains, KS47, which removed 90.4% cutting oil in 48 hr, was obtained by screening test under aerobic conditions(pH 7, $28^{\circ}C$). KS47 was identified as Pseudomonas aeruginosa according to morphological, physiological and biochemical properties, 16S rDNA sequence, and fatty acid analysis. P. aeruginosa KS47 could utilize cutting oil as carbon source. In batch test, we obtained optimal degradation conditions(1.5 g/L cell concentration, pH 7, and temperature $30^{\circ}C$). Under the optimal conditions, 1,060 mg/L cutting oil was removed 83.7% (74.1 mg/L/hr). 본 실험은 생분해가 어려운 절삭유를 단일 균주에 의해 생물학적 처리를 하는 데에 목적이 있다. 절삭유, 절삭폐유로부터 호기 균주 81개를 분리하여 이중 절삭유 분해능이 가장 높은 균주로, 48시간 내에 90.4%를 제거한(초기농도 699.1 mg/L) KS47을 선별하였다. KS47은 형태학적, 생리 화학적, 16S rDNA 염기서열, 그리고 지방산 분석을 통해 Pseudomonas aeruginosa로 동정되었다. P. aeruginosa KS47은 절삭유를 탄소원으로 사용하여 성장 할 수 있었으며, 절삭유 분해시, 최적 분해 조건은 1.5 g/L(wet weight), pH 7.0, $30^{\circ}C$이었다. 최적 조건 하에서 절삭유의 제거능을 본 결과, 1,060 mg/L의 절삭유를 12시간 내에 83.7% 제거함을 확인하였다.

카오스모스 시학과 김지하 담시의 구조형성방식 -「비어(蜚語)」와 「똥바다」를 중심으로

김란희 ( Kim Ran-hee ) 국제어문학회 ( 구 국제어문학연구회 ) 2009 국제어문 Vol.45 No.-

본고는 김지하 담시의 구조형성 방식을 카오스모스 시학으로 살펴보고자 한다. 여기서 언급하는 카오스모스 시학이란 현대시학이 추구하는 다의성의 본질을 이해하기 위해서 현대 과학이 다루고 있는 우연성, 불확정성, 개연성, 애매모호함, 다가치성 등을 예술에 접목시켜 시학적 지평을 학제간의 영역으로 넓혀보고자 하는 방법론이라 할 수 있다. 카오스와 복잡성에 대한 현대 과학의 발전은 하나의 시학을 가능하게 하는데, 그것은 불연속성, 복잡성, 자기 조직화 이론으로 대변되는 카오스모스 시학으로 문학 텍스트의 복잡성과 밀도, 결 등을 은유적으로 살펴볼 수 있는 하나의 도구가 될 수 있다. 이는 김지하 시인 스스로가 담시 발표 이후 꾸준히 카오스모스와 자기조직화의 시론을 내세우며 민중의 노래(민요와 판소리, 각설이 타령, 배뱅이 굿 등)가 대표적인 카오스모스와 자기 조직화의 사상을 보여주는 예술장르라고 밝힌 점과도 일치 한다. 본고에서 민중 문학이 현실을 반영하는 지시적 코드로만 평가받았던 연장선에서 벗어나 김지하 텍스트에 대한 시학적 접근의 도구로 카오스모스 시학을 택했던 것은 김지하의 담시 텍스트가 지니고 있는 지시성과 애매성, 복잡성, 다층성 등을 동시에 살펴볼 수 있다는 점에 주목했기 때문이다. 이는 김지하 담시에서 감지되는 혼돈과 질서, 질서와 혼돈이라는 역설적 정합의 미학을 시학적 차원에서 규명해보고자 하는 작은 시도라 할 수 있다. <불연속성과 복잡성>, <자기 방해의 패턴>, <자기조직화의 원리>로 대표되는 카오스모스 시학을 통해 살펴본 김지하 담시는 불연속성과 복잡성의 조우를 통해 선형적이고 직선적인 줄거리로부터 벗어난 것처럼 보이지만 나름대로의 질서를 형성하면서 전체성에 복무하고 있는 다층성을 지니고 있다. 이는 `혼돈 속의 질서`라는 역설적 정합 과정을 통한 담론의 조직화로, 기왕의 민중 문학이 지녀왔던 단성성의 한계를 뛰어넘는 새로운 시적 창조를 보여줌과 동시에 오히려 메시지를 한층 더 강화해내는 효과를 낳고 있다. 또한 모순 어법을 통해 모순적인 의미의 충돌과 혼합을 통한 언어의 테크닉을 보여주고 있는데, 이는 풍자의 결을 다층적으로 보여주는 효과를 낳고 있다. 이는 서로 다른 언어의 이질성이 융합되어 제 3의 의미를 생성해내는 시 담론의 조직화 방식으로도 볼 수 있다. 이에 따라 텍스트의 다의미성은 형성되며 독자들은 당시의 시대 상황을 다층적으로 지각하게 된다. 아울러 담시를 이루고 있는 각각의 분리된 이야기는 스스로 자기 조직화를 꾀하면서 더 넓은 영역 안에서 통합되고 있는데, 그것은 마치 단순한 것들이 에너지를 이용해 결합하면서 복잡한 것으로 변해가는 자기 조직화 논리와 맞닿은 것으로, 우연적 요소가 필연적 기제로 작동을 하고 있는 것이다. 그 안에서 각각의 요소는 불연속성과 복잡성이라는 상호 교호 작용을 통해 자기 조직화를 이루어내고 있다. 이에 따라서 김지하 담시 텍스트에서 그동안 읽혀왔던 지배/ 피지배, 억압/피억압, 권력층/민중의 이분법적 대립 구도는 이러한 분리와 결합의 간섭과 충돌 과정을 통한 열림과 닫힘의 끊임없는 언어적 실험으로 다시 읽을 수 있는 것이다. This study intends to explore into the Kim Ji-ha`s lay structure-forming method in terms of "chaosmos poetics". The chaosmos poetics refers to a method that integrates the accidentality, indeterminacy, probability, ambiguity and muti-valuableness which modern poetics addresses with art and attempts to expand the poetics horizon to the interdisciplinary sphere in order to understand the essence of the equivocality which modern poetics seeks. The development of the modern science on chaos and complexity made one poetics possible, which could be a tool which would metaphorically look to the complexity, density and texture of literary texts with a chaosmos poetics represented by discontinuity, complexity and self-organization theories. This coincides with an assertion that poet Kim Ji-ha has claimed that popular songs("minyo", or folk song, "pansori", or traditional Korean narrative song, "gakseolitaryeong" or singing beggar`s ballards and "Baebaengi gut", or Baebangi`s shaman ritual, etc) are the art genre showing the thought of the representative chaosmos and self-organization while steadily promoting the poetics of chaosmos and self-organization since the release of the selfproclaimed narrative poetry. In this study, the reason why I chose the chaosmos poetics as a tool for the poetic approach to Kim Ji-ha texts, deviating from the prolongation that has been only evaluated as a reference code which popular literature reflects reality is because I looked to a point that I could simultaneously examine the reference, ambiguity, complexity and multi-layeredness which his lay texts carry. This could be a little attempt that tries to explore into the paradoxically thesis-antithesis aesthetics of chaosmos vs order and order vs. order which is sensed in Kim Ji-ha lay from the perspective of poetics. The Kim Ji-ha lay which were investigated via chaosmos poetics represented by "discontinuity and complexity", "self-obstructive pattern", and "self-organization principle", seem to be deviated from a curved and linear storyline via an encounter of discontinuity and complexity but have their-own multi-layeredness that both shapes order and serves to wholeness. This is both an organization of the lay via a paradoxical thesis-antithesis process of the so-called "order in chaos" and a mani -festation of the new poetic creation beyond the limit of the unisexuality which the past popular literature held, simultaneously giving rise to an effect that enforces a message much more. And Kim Ji-ha lay show a language technique through the conflict and mix of contradictory meanings via a oxymoron, which brings about an effect that demonstrates the texture of satires in a layeredness way. It would be a poetic lay-forming way which generates the third meanings as a result of the heterogeneity of different languages being converged each other. Thus, the multi-meaningfulness of the text is formed and readers perceive their contemporary multi-layered circumstances. Further, the differently separate stories which consist of a lay seeks a self- organ -ization on their own and are integrated into a wider sphere, which is connected to the logic of the self-organization of as if simple objects are combined each other by using an energy and transformed into a complex thing, accidental elements functioning as a necessary mechanism. As it were, each element seeks a self-organization via an interaction of discon -tinuity and complexity in the mechanism. Hence, the dichotomical confrontation scheme of the ruling/the ruled, the suppressing/the suppressed and power class/the populace that have been read in the Kim Ji-ha lay texts thus far would be newly read with a ceaseless language experiment of opening and closing via the inter -ference and conflict of such separation and combination.

김지하 담시에 나타난 "신명"과 시적 언어의 부정성

김란희 ( Ran Hee Kim ) 시학과 언어학회 2011 시학과 언어학 Vol.21 No.-

This study is to examine the language used by Kim Ji-ha in his poems through the theory of "Sinmyeong", a concept that the Korean poet himself defines as the ultimate value of public language. Kim brought his poetic language in his lays to the public discourse by his linguistic faith in the concept of Sinmyeong, so that he was able to suggest the poetic social norm about the nature and form of public language. He identifies Sinmyeong with a core value that unites all the values that the public pursue, and his Sinmyeong provides a coordinate of the literature of his time, regarding what the public language was, and thereby provides the key to understand his creation principle and language characteristics. According to the Korean poet, "Sinmyeong" refers to gigantic literary embodiment of languages that breathe and keep their rhythms and rhyme, and thus it revives the physical impulse that have long been forgotten in the symbolic system of language. How is Sinmyeong, the gigantic literary embodiment of the public language, represented in texts? Sinmyeong is literary physicality and the energy of body and the movement of psychological energy. It is physically expressed by sound or rhythm, and concurrently contradicting the symbolic language system. Therefore, this study is to identify the poetic principle of Sinmyong through the concept of negativity of the poetic language that Julia Kristeva suggested. The negativity of the poetic language delivered by Kim Ji-ha`s lays is represented by repeating rhythms and phrases, adopting onomatopoeic and mimetic words, and breaking or distorting morphologic-syntactic rules. Such signs of negativity are the purses of the sign system in order to change the physical impulse of the narrators of his poems, and to confront meanings of the symbolic system. By doing so, poetic languages relieve and energize the suppressed public, and express social resistance. Through his effort and the kind, the public of his time fully enjoy Sinmyeong that reinvigorated their spirit and energy.

해수 내 AOC(동화가능 유기탄소)가 바이오 파울링에 미치는 영향 평가

김란희 ( Lan Hee Kim ),김성조 ( Sung Jo Kim ),정상현 ( Sang Hyun Jeong ),김창민 ( Chang Min Kim ),신명섭 ( Myung Seop Shin ),김인수 ( In S. Kim ) 한국물환경학회(구 한국수질보전학회) 2011 공동 추계학술발표회 Vol.2011 No.-

하수기반 역학 바이오 모니터링을 통한 COVID-19 조기검출

김란희 ( Kim Lan Hee ),김상국 ( Kim Sangkook ),윤효직 ( Yoon Hyojik ),김성표 ( Kim Sungpyo ) 한국물환경학회 2020 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2020 No.-

Wastewater-based epidemiology (WBE) monitoring tool has been used to detect illicit drugs and environmental contaminants in wastewater samples collected from the influent of wastewater treatment plants (WWTPs). The WBE can provide spatial and temporal information about the community footprint in the living area. As the coronavirus (COVID-19) disease, which is caused by severe acute respiratory syndrome coronavirus (SARS-CoV) become pandemic in 2019, many countries have employed the WBE monitoring tool enabling early-detection or monitoring of the infected cases (symptomatic and non-symptomatic) in the community without disturbance of individual privacy. However, various COVID-19 detection methods applied in studies made difficulties in method standardization. Besides, the extraction efficiency is depending on the virus properties and the water types. Therefore, evaluation of developed detection methods and data quality analysis should be required. We evaluated and optimized COVID-19 detection methods in the real wastewater influent and sludge samples. To detect COVID-19, four different extraction methods, absorption-elution ultrafiltration, ultracentrifugation, centrifugation/ultrafiltration, and PEG precipitation, have been compared. The viral RNA was extracted using a QIAamp^® viral RNA mini kit (Qiagen, USA), and the COVID-19 was detected using a GoTaq^® probe 1-step RT-qPCR (Promega, USA) with two COVID-19 nucleocapsid-specific primers, N1 and N2. The viral community in the samples was analyzed through a NGS-based metagenomic sequencing. This study would help to make a guideline of the COVID-19 detection standard method and to develop quality assurance (QA) and quality control (QC) protocol.

2009-2011년에 발생한 신종 인플루엔자 A (H1N1)의 신경학적 양상

김란희(Ran Hee Kim),김영미(Young Mi Kim),박수은(Soo Eun Park),김혜영(Hye Young Kim),이윤진(Yoon Jin Lee),김태형(Tae Hyoung Kim),남상욱(Sang Ook Nam) 대한소아신경학회 2011 대한소아신경학회지 Vol.19 No.1

목적 : 신종 인플루엔자 A (H1N1) 바이러스 감염의 신경학적 합병증의 발생 빈도를 확인하고 임상적 고찰을 시행하였다. 방법 : 2009년 7월부터 2011년 1월까지 부산대학교 병원의 외래, 입원 및 응급실에서 신종 인플루엔자 A (H1N1) RT-PCR로 감염이 확인된 만 18세 이하의 752명 환자의 의무기록을 검토하였다. 이들 중 신경학적 합병증을 보인 환자를 대상으로 인플루엔자의 임상 증상, 신경학적 이상 증상, 검사실소견, 뇌파검사 결과, 신경 방사선학적 검사 소견 등을 조사하였다. 결과 : 15명의 환자가 신경학적 증상을 보였으며 평균 연령은 8.8세이었다. 남아가 10명 (66.7%), 여아가 5명 (33.3%)이었다. 9명 (60)이 경련을 보였고, 2명(13.3%)이 뇌염, 2명(13.3%)가 환시, 1명 (6.7%)이 무균성 수막염, 1명(6.7%)이 현훈을 보였다. 급성 열성 감염의 발생 평균 2일(범위 0-4일)이 경과한 후 신경학적 증상을 보였다. 3명 (20.0%)이 뇌척수액 검사에서 이상 소견을 보였으나 신종인플루엔자 A (H1N1)은 검출되지 않았다. 1명 (6.7%)이 뇌 자기공명검사에서 이상 소견을 보였다. 14명 (93%)이 oseltamivir를 투여 받았다. 모든 환자가 완전히 회복되었으며 신경학적 후유증은 없었다. 결론 : 신종 인플루엔자 A (H1N1)는 대유행 기간 동안 다양한 신경학적 증상을 동반하였다. 소아 신경학을 진료하는 의사는 인플루엔자의 유행시기에 발생하는 신경학적 증상의 감별진단으로 인플루엔자 감염을 반드시 고려해야 하겠다. Purpose : To describe the characteristics and incidence of neurologic complications related to the novel influenza A (H1N1) virus. Methods : We reviewed the medical records of 752 children and adolescence (≤ 18 years of age) who had been diagnosed as novel influenza A (H1N1) infection through positive results of influenza A (H1N1) RT-PCR and hospitalized or visited the outpatient clinic and emergency department of Pusan National University Hospital from July 2009 to January 2011. Results : We identified 15 patients who had experienced a neurologic symptom with a mean age of 8.8 years. There were 10 (66.7%) males and five (33.3%) females. Nine patients (60.0%) presented with seizures, two (13.3%) with decreased mentality, two (13.3%) with visual hallucination, and one (6.7%) with vertigo. The mean duration from onset of respiratory illness to the beginning of neurologic symptoms was 2 days (range: 0-4 days). Three patients (patient 2, 7, and 13) (20.0%) had abnormal results on cerebrospinal fluid analysis; however, novel influenza A (H1N1) was not detected. Further, one patient (6.7%) had abnormal MRI. Antiviral therapy (oseltamivir) was administered to fourteen patients (93%) and all patients recovered fully and had no neurologic sequelae. Conclusion : Novel influenza A (H1N1) was a cause of neurologic symptoms during the outbreak. Pediatricians should consider influenza virus infection in the differential diagnosis for children with neurologic symptoms during an epidemic of influenza.

하수기반 역학 바이오 모니터링을 통한 COVID-19 조기검출

김란희 ( Kim Lan Hee ),김상국 ( Kim Sangkook ),윤효직 ( Yoon Hyojik ),김성표 ( Kim Sungpyo ) 한국물환경학회 2020 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2020 No.-

Wastewater-based epidemiology (WBE) monitoring tool has been used to detect illicit drugs and environmental contaminants in wastewater samples collected from the influent of wastewater treatment plants (WWTPs). The WBE can provide spatial and temporal information about the community footprint in the living area. As the coronavirus (COVID-19) disease, which is caused by severe acute respiratory syndrome coronavirus (SARS-CoV) become pandemic in 2019, many countries have employed the WBE monitoring tool enabling early-detection or monitoring of the infected cases (symptomatic and non-symptomatic) in the community without disturbance of individual privacy. However, various COVID-19 detection methods applied in studies made difficulties in method standardization. Besides, the extraction efficiency is depending on the virus properties and the water types. Therefore, evaluation of developed detection methods and data quality analysis should be required. We evaluated and optimized COVID-19 detection methods in the real wastewater influent and sludge samples. To detect COVID-19, four different extraction methods, absorption-elution ultrafiltration, ultracentrifugation, centrifugation/ultrafiltration, and PEG precipitation, have been compared. The viral RNA was extracted using a QIAamp^® viral RNA mini kit (Qiagen, USA), and the COVID-19 was detected using a GoTaq^® probe 1-step RT-qPCR (Promega, USA) with two COVID-19 nucleocapsid-specific primers, N1 and N2. The viral community in the samples was analyzed through a NGS-based metagenomic sequencing. This study would help to make a guideline of the COVID-19 detection standard method and to develop quality assurance (QA) and quality control (QC) protocol.

하수역학 분석방법의 전환

김란희 ( Kim Lan Hee ),박민승 ( Park Minseung ),김수연 ( Kim Sooyeon ),김성표 ( Kim Sungpyo ) 한국물환경학회 2024 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2024 No.-

하수기반 역학 바이오 모니터링을 통한 COVID-19 조기검출

김란희 ( Kim Lan Hee ),김상국 ( Kim Sangkook ),윤효직 ( Yoon Hyojik ),김성표 ( Kim Sungpyo ) 한국물환경학회 2020 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2020 No.-

Wastewater-based epidemiology (WBE) monitoring tool has been used to detect illicit drugs and environmental contaminants in wastewater samples collected from the influent of wastewater treatment plants (WWTPs). The WBE can provide spatial and temporal information about the community footprint in the living area. As the coronavirus (COVID-19) disease, which is caused by severe acute respiratory syndrome coronavirus (SARS-CoV) become pandemic in 2019, many countries have employed the WBE monitoring tool enabling early-detection or monitoring of the infected cases (symptomatic and non-symptomatic) in the community without disturbance of individual privacy. However, various COVID-19 detection methods applied in studies made difficulties in method standardization. Besides, the extraction efficiency is depending on the virus properties and the water types. Therefore, evaluation of developed detection methods and data quality analysis should be required. We evaluated and optimized COVID-19 detection methods in the real wastewater influent and sludge samples. To detect COVID-19, four different extraction methods, absorption-elution ultrafiltration, ultracentrifugation, centrifugation/ultrafiltration, and PEG precipitation, have been compared. The viral RNA was extracted using a QIAamp^® viral RNA mini kit (Qiagen, USA), and the COVID-19 was detected using a GoTaq^® probe 1-step RT-qPCR (Promega, USA) with two COVID-19 nucleocapsid-specific primers, N1 and N2. The viral community in the samples was analyzed through a NGS-based metagenomic sequencing. This study would help to make a guideline of the COVID-19 detection standard method and to develop quality assurance (QA) and quality control (QC) protocol.

Membrane autopsy를 통한 BWRO (Brackish Water Reverse Osmosis) 막오염에 대한 연구

김성조 ( Sung Jo Kim ),김란희 ( Lan Hee Kim ),김창민 ( Chang Min Kim ),신명섭 ( Myung Seop Shin ),유혜원 ( Hye Weon Yu ),오병수 ( Byung Soo Oh ),계정일 ( Jeong Il Kye ),김민정 ( Min Joung Kim ),김인수 ( In S. Kim ) 한국물환경학회(구 한국수질보전학회) 2011 공동 추계학술발표회 Vol.2011 No.-