초등학교 실내환경에서 공기 중 세균과 진균의 분리 및 특성

김나영,김영란,김민규,조두완,김종설,Kim, Na-Yeong,Kim, Young-Ran,Kim, Min-Kyu,Cho, Du-Wan,Kim, Jong-Seol 한국미생물학회 2007 미생물학회지 Vol.43 No.3

울산 소재 초등학교 3곳의 교실과 복도에서, 배양가능한 공기중 세균과 진균의 농도를 조사하고, 이들 미생물을 분리한 후 동정하였다. 세균과 진균의 포집에는 충돌식 공기 채취기를 사용하였으며, 세균수와 진균수의 측정에는 각각 plate count agar와 dichloran rose bengal chloramphenicol agar를 사용하였다. 학기 중 세균 농도는 교실에서 $168{\sim}3,887MPN/m^3$ 복도에서 $168{\sim}6,339MPN/m^3$의 범위였으며, 진균 농도는 교실에서 $34{\sim}389MPN/m^3$, 복도에서 $91{\sim}507MPN/m^3$의 범위로, 상황과 학교에 따른 측정값의 편차는 세균에서 진균보다 더 크게 나타났다. 분리한 세균의 84%는 그람양성으로 관찰되었는데, 전체 시험한 세균의 61%는 Micrococcus 속으로, 이중 75%는 M. luteus로 확인되었으며, Staphylococcus속은 전체의 10%수준이었다. Micrococcus 속의 주요 발생원은 사람으로 생각되며, 함유한 색소나 높은 세포벽 함량 등의 생리적 특징이 이들 세균의 공기중 생존력을 높이는 것으로 추측된다. 포집한 시료로부터 15속의 사상성 진균을 확인할 수 있었으며, Cladosporium 속, Aspergillus 속, Penicillium속 등이 분리한 진균의 69%를 차지하였다. 1개 학교의 교실에서는 Stachybotrys속이 검출되었는데, 이 속의 S. chartarum은 많은 진균독소를 생산하는 것으로 잘 알려져 있다. 독소생산과 관련이 깊은 Aspergillus 속, Penicillium속, Stachybotrys 속에 대한 종 수준에서의 동정, 분리한 진균의 독소생산능력, 공기중 진균독소 검출 및 진균 농도와의 상관관계 분석 등에 대한 보다 체계적인 연구가 필요하다고 판단된다. Indoor airborne bacterial and fungal concentrations were examined at classrooms and corridors of 3 elementary schools in Ulsan. Airborne microorganisms were collected with an impaction-type air sampler using plate count agar and dichloran rose bengal chloramphenicol agar. During the semester, concentrations of bacteria ranged $168{\sim}3,887 MPN/m^3$ at classrooms and $168{\sim}6,339 MPN/m^3$ at corridors, while those of fungi ranged $34{\sim}389 MPN/m^3$ at classrooms and $91{\sim}507 MPN/m^3$ at corridors. The bacterial concentrations showed larger variations between situations and schools compared to those of fungi. When airborne bacteria were isolated and identified, 84% were observed as Gram-positive, and Micrococcus spp. was the most abundant group with 61% of tested isolates, followed by genus Staphylococcus with 10%. The Micrococcus spp. isolates, of which 75% were identified as M. luteus, appeared to be from human origins. The protective pigments and substantial cell wall of Micrococcus may provide selective advantage for their survival in the air. We also isolated and identified 15 genera of filamentous fungi. The most common culturable fungi were Cladosporium, Aspergillus and Penicillium, and these 3 genera were 69% of fungal isolates. Genus Stachybotrys, of which S. chartarum is a well known producer of many potent mycotoxins, was also detected from one of the schools. further systematic studies are necessary with an emphasis on species identification and mycotoxin production of isolated fungal genera, including Aspergillus, Penicillium, and Stachybotrys.

충남 일부 지역 대학생의 거주형태에 따른 식생활에 관한 연구

김나영,김성환,김은미,Kim Na-Young,Kim Sung-Hwan,Kim Eun-Mi 동아시아식생활학회 2006 동아시아식생활학회지 Vol.16 No.1

This study examined the dietary life by residence type of 409 university students (198 male and 211 female) in Chungnam They were divided into five groups according their residence: living in the family home a short distance from the university, living in the family home a long distance from the university, boarding house, self-boarding house, and university dormitory. In this study, the intake of food and nutrients and health, and dietary behaviors of each group were investigated through two-week questionnaire. The average age, height, weight, and BMI were 21.4, 175.3 cm, 68.9 kg, 22.3 in males, and 19.9, 162.3 cm, 53.3 kg, 20.2 in females, respectively. Dietary attitude of the students living in home was higher than that of the other student groups. The rate of breakfast intake and the frequency of eating 10 food items of subjects except processed food of self-boarding house group were lower than those of the other 4 student groups. These results suggest that university students of self-boarding house might have low ability of meal management and more reasonable nutrition education is needed for desirable food behaviors.

용담의 RAW 264.7 세포주에서의 Nitric Oxide 생성 저해물질

김나영,강태현,김도훈,김윤철,Kim, Na-Young,Kang, Tai-Hyun,Kim, Do-Hoon,Kim, Youn-Chul 한국생약학회 1999 생약학회지 Vol.30 No.2

Bioassay-guided fractionation of a $H_2O$ extract of the roots of Gentiana scabra has furnished 5-(hydroxymethyl)-2-furfural (1) as an inhibitory compound for nitric oxide (NO) production in murine macrophage RAW 264.7 cells stimulated with $interferon-{\gamma}$ plus lipopolysaccharide. Compound 1 showed the moderate inhibition of NO production with $IC_{50}$ value of $803\;{\mu}M$.

Reduced Ceramides Are Associated with Acute Rejection in Liver Transplant Patients and Skin Graft and Hepatocyte Transplant Mice, Reducing Tolerogenic Dendritic Cells

김나영,Hyun Ju Yoo,Yeogyeong Yi,Yoorha Kang,Su Jung Kim,Young-In Yoon,Phuc Huu Tran,Taewook Kang,Min Kyung Kim,Jaeseok Han,Eunyoung Tak,Chul-Soo Ahn,Gi-Won Song,Gil-Chun Park,Sung-Gyu Lee,Jae-Joong Kim,D 한국분자세포생물학회 2023 Molecules and cells Vol.46 No.11

We set up this study to understand the underlying mechanisms of reduced ceramides on immune cells in acute rejection (AR). The concentrations of ceramides and sphingomyelins were measured in the sera from hepatic transplant patients, skin graft mice and hepatocyte transplant mice by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Serum concentrations of C24 ceramide, C24:1 ceramide, C16:0 sphingomyelin, and C18:1 sphingomyelin were lower in liver transplantation (LT) recipients with than without AR. Comparisons with the results of LT patients with infection and cardiac transplant patients with cardiac allograft vasculopathy in humans and in mouse skin graft and hepatocyte transplant models suggested that the reduced C24 and C24:1 ceramides were specifically involved in AR. A ceramide synthase inhibitor, fumonisin B1 exacerbated allogeneic immune responses in vitro and in vivo, and reduced tolerogenic dendritic cells (tDCs), while increased P3-like plasmacytoid DCs (pDCs) in the draining lymph nodes from allogeneic skin graft mice. The results of mixed lymphocyte reactions with ceranib-2, an inhibitor of ceramidase, and C24 ceramide also support that increasing ceramide concentrations could benefit transplant recipients with AR. The results suggest increasing ceramides as novel therapeutic target for AR, where reduced ceramides were associated with the changes in DC subsets, in particular tDCs

의약품의 정보디자인 시스템 구축을 위한 프레임워크

김나영,장동련,Kim, Na-Yeong,Jang, Dong-Ryeon (사)한국포장협회 2020 包裝界 Vol.330 No.-

공과대학 캡스톤 디자인의 학습성과에 대한 자기조절학습전략의 매개효과 검증

김나영,이소영,Kim, Na-Young,Lee, So Young 한국공학교육학회 2017 공학교육연구 Vol.20 No.5

This study aimed to identify the causal relationships among self-regulated learning strategy, problem solving efficacy, task value and learning outcome, and mediation effect of self-regulated learning strategy in engineering capstone design course. The data were collected from 363 university students who enrolled in capstone design courses and analyzed using structural equation modeling method. The results were: first, problem-solving efficacy and task value exerted significant effects on self-regulated learning strategy. Second, self-regulated learning strategy exerted significant effects on learning outcome, but problem-solving efficacy and task value did not. Third, problem-solving efficacy and task value showed significant indirect effects on learning outcome, which confirmed that self-regulated learning strategy fully mediated between two exogenous variables and learning outcome.

북한의 건설사례 - 금강산에서의 건설경험

김나영,Kim, Na-Yeong 대한건축사협회 2000 建築士 Vol.2000 No.4

대전지역 유치원 종일반, 반일반 유아와 이들 어머니의 식생활 비교

김나영,김성환,임지희,Kim Na-Young,Kim Sung-Hwan,Lim Ji-Hee 동아시아식생활학회 2006 동아시아식생활학회지 Vol.16 No.1

The purpose of this study was to provide basic information need to promote early childhood's growth with balanced diet and preferable dietary attitude by observing the provision of food service management in kindergarten in Daejeon. We researched the dietary life of full- and half-day program children and their mothers. A questionnaire survey was conducted with 392 children attending kindergarten and their mothers. The questionnaire items examined general characteristics, dietary life, dietary behavior, frequency food intake etc. The results of the survey were as follows. The frequency of breakfast intake of the half-day program children and their mothers was higher than that of the full-day program children and their mothers. Most of the children had an unbalanced diet about soybean, kimchi, and vegetables, whereas their mothers had an unbalanced diet about processed food In general, the half-day program children showed higher scores than full-day children for the items of the dietary behavior of kindergarten. The dietary attitude and nutrition attitude scores of the half-day program children and their mothers were higher than those of the full-day program children and their mothers.

홀로그래픽 데이터 저장 시스템을 위한 2차원 코드

김나영,이재진,Kim, Na-Young,Lee, Jae-Jin 한국통신학회 2006 韓國通信學會論文誌 Vol.31 No.11C

본 논문에서는 차세대 대용량 데이터 저장장치로 부각되고 있는 홀로그래픽 데이터 저장장치를 위한 2차원 변조코드를 제안한다. 단위 면적당 기록 밀도의 증가로 인한 인접 심볼간 간섭과 3차원적인 홀그래픽 저장으로 인한 페이지간 섭 등에 강인한 성능을 갖도록 같은 페이지 내의 1과 0의 개수가 거의 동일하도록 설계하였으며, 또 가능한 많은 1과 0의 천이가 일어나도록 설계하였다. 제안된 코드의 코드율은 5/9로서 기존의 4/9 코드보다 25%의 코드율 개선을 하였다. In this paper, we introduce a two-dimensional modulation code for holographic data storage systems(HDSS), which is a candidate for the next generation data storage system. The two-dimensional(2D) intersymbol interference(ISI) induces higher bit error rate(BER). The balanced number of zeros(dark) and ones(light) in each page reduces inter-page interference(IPI). The code rate is 519. Although the proposed code has higher code rate than other 2D code with rate 4/9, the BER performances of two codes are similar.

홍삼 분말 첨가 식빵의 이화학적 및 관능적 특성

김나영,김성환,Kim Na-Young,Kim Sung-Hwan 동아시아식생활학회 2005 동아시아식생활학회지 Vol.15 No.2

This study was conducted to investigate the quality characteristics of the bread with red ginseng powder(RG). Four different powder concentration levels of 0, 3, 6, and $9\%$ were added to flour to make the bread. The appearance, pH, water holding capacity, specific volume, color, texture, and sensory properties were analyzed. pH of bread with RG had no difference according to the content of the red ginseng powder. The specific volume of the bread containing $0,\;3,\;6\%$ were bigger than that of the bread with $RG-9\%$. The water holding capacity of the bread with $RG-9\%$ was the highest. As the amounts of RG increased, L-values of the bread crumb were decreased and a- and b-values were increased. As a result of texture measuring by texture analyzer, hardness and gumminess were not affected by the addition of RG. Also, springiness, cohesiveness, and chewiness were not significantly different among the control, $RG-3\%\;and\;RG-9\%$. In sensory evaluation, color, flavor, taste and overall acceptability in the bread with $RG-9\%$ were significantly lower than the control and the other groups. On the other hand, the best result for sensory characteristics showed when red ginseng powder at $3\%$ level was added to the bread.