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김두환,이재신,함희진,기노준,이정학,Kim Doo-Hwan,Lee Jae-Shin,Ham Hee-Jin,Ki No-Joon,Lee Jung-Hark 한국동물위생학회 2004 韓國家畜衛生學會誌 Vol.27 No.3
It was carried out to compare the residual materials by EEC 4-plate, Charm II and HPLC method in the muscles of cattle and pigs from slaughter-houses in Seoul from 2000 to 2003. Residual materials were detected from $1.10\%$(73/6,623) samples by EEC 4-plate method, and $10.93\%$(55/503) samples by Charm II method. The highest residual concentration(ppm) of oxytetracycline, tetracycline, chloramphenicol, chlortetracycline, sulfamethazine, sulfamerazine, sulfadimethoxine, penicillin and sulfamonomethoxine were 25.5, 3.46, 3.26, 1.5, 0.3, 0.2, 0.2, 0.14, and 0.07, respectively. Eighty nine samples were classified as 58($65.17\%$) only tetracyclines, 20($22.47\%$) only sulfonamides, 3($3.37\%$) only ${\beta}$-lactams, 2($2.25\%$) only chloramphenicol, 4($4.49\%$) tetracyclines and sulfonamides simultaneously, 1($1.12\%$) chloramphenicol and sulfonamides simultaneously, and 1($1.12\%$) chloramphenicol, sulfonamides and tetracyclines simultaneously.
김은 ( Eun Kim ),나인택 ( In Taek Ra ),기노준 ( Rho Jun Gi ),이정학 ( Jung Hark Lee ) 한국가축위생학회 2004 韓國家畜衛生學會誌 Vol.27 No.1
It was conducted to evaluate the microbiological quality on the surface of slaughtered beef and pork products in Seoul from January 2003 to December 2003. Two hundreds four beefs and 284 pork carcasses were surveyed on generic E coli, total bacterial count for microbiological quality, and Salmonella spp as food-borne pathogen. The prevalence of the excellent or good grade(104 CFU/cm2 in SPC) of beef and pork carcasses were 99.7% and 97.9%, respectively. The frequency of beef carcasses with less than 102 CFU/cm2 of E coli was 100%, while that of pork carcasses was 98.2%. Salmonella spp was not recovered from all of beef and pork carcasses.
서울지역에서 도축된 식육의 미생물 오염도 및 병원성 미생물 검사
김주영 ( Ju Young Kim ),이주형 ( Ju Hyung Lee ),기노준 ( No Jun Gi ),이정학 ( Jung Hak Lee ) 한국가축위생학회 2005 韓國家畜衛生學會誌 Vol.28 No.3
The bacteria on the surface of slaughtered meat was monitored to investigate the relationships between microbiological quality and sanitation management in slaughter process of cattle and pig. It was conducted to evaluate the microbiological quality on the surface of slaughtered beef and pork in Seoul from January to December 2004. Two hundred and thirty three beef and 233 pork carcasses were surveyed on generic E coli counts and standard plate count for microbiological quality and Salmonella spp, Listeria monocytogenes, Staphylococcus aureus, Clostridium perfringens and E coli O157:H7 as pathogenic microorganisms. The prevalence of the excellent or good grade(104 CFU/cm2) in beef and pork carcasses were 100% and 99.2%, respectively. The frequency of beef carcasses with less than 102 CFU/cm2 of generic E coli counts was 100%, while that of pork carcasses was 99.6%. Of 233 beef carcasses, 1(0.42%) was contaminated with L monocytogenes and 6(2.58%)with C perfringens. Of 233 pork carcasses, 11(4.72%), 2 (0.86%), and 2 (0.86%) were contaminated with L monocytogenes, C perfringens, and S aureus, respectively. Salmonella spp and E coli O157:H7 were not detected with all of the beef and pork carcasses. In conclusion, this study emphasized the importance of relationship between microbiological quality and sanitation management in slaughter process of cattle and pig, in abattoirs.
서울 시내 도축장의 소,돼지 지육에 대한 잔류물질(tetracyclines, β-Lactams, chloramphenicol, sulfonamides) 함량 조사
김두환 ( Doo Hwan Kim ),이재신 ( Jae Shin Lee ),함희진 ( Hee Jin Ham ),기노준 ( No Joon Ki ),이정학 ( Jung Hark Lee ) 한국가축위생학회 2004 韓國家畜衛生學會誌 Vol.27 No.3
It was carried out to compare the residual materials by EEC 4-plate, Charm Ⅱ and HPLC method in the muscles of cattle and pigs from slaughter-houses in Seoul from 2000 to 2003. Residual materials were detected from 1.10%(73/6,623) samples by EEC 4-plate method, and 10.93%(55/503) samples by Charm Ⅱ method. The highest residual concentration(ppm) of oxytetracycline, tetracycline, chloramphenicol, chlortetracycline, sulfamethazine, sulfamerazine, sulfadimethoxine, penicillin and sulfamonomethoxine were 25.5, 3.46, 3.26, 1.5, 0.3, 0.2, 0.2, 0.14, and 0.07, respectively. Eighty nine samples were classified as 58(65.17%) only tetracyclines, 20(22.47%) only sulfonamides, 3(3.37%) only β-lactams, 2(2.25%) only chloramphenicol, 4(4.49%) tetracyclines and sulfonamides simultaneously, 1(1.12%) chloramphenicol and sulfonamides simultaneously, and 1(1.12%) chloramphenicol, sulfonamides and tetracyclines simultaneously.
서울지역의 도축 소,돼지 지육에서의 잔류물질 비교 조사
임홍규 ( Hong Kyu Lim ),최태석 ( Tae Suk Choi ),윤은선 ( Eun Sun Yun ),이주형 ( Joo Hyung Lee ),기노준 ( No Joon Ki ),이정학 ( Jung Hark Lee ) 한국가축위생학회 2003 韓國家畜衛生學會誌 Vol.26 No.2
This study was carried out to compare the antibiotic residues in the muscles of cattle and pigs from slaughter-houses in Seoul from 2000 to 2002 by EEC-4-plate, Charm Ⅱ and l-IPLC methods. The results were summarized as follows; 1. Residual materials were detected from 41 samples(0.6%) by EEC-4-plate method from random sampling and 38 samples(12%) by Charm Ⅱ method from directed sampling. 2. Violation rates were 0.3% by monitoring and 4.7% by surveillance program. 3. The 35 samples were classified as tetracyclines 30(86%), sulfonamides 4(11%), b-lactams 1(3%) and two samples simultaneously determined oxyteracycline plus sulfadimetoxine, and sulfamerazine plus sulfadimetoxine. 4. The highest residual concentration(ppm) of chiortetracycine, oxytetracyclirie, sulfamethazine, sufadimetoxine and penicillin were 0.5, 12.0, 6.4, 2.6 and 0.44, respectively.
서울지역 어린이 놀이터 모래에서 개 회충류의 충란 조사
조미영 ( Mi Yeong Jo ),강승원 ( Seung Won Kang ),채희선 ( Hee Sun Chae ),김두환 ( Doo Hwan Kim ),배내수 ( Nae Soo Bae ),기노준 ( No Joon Ki ),이병동 ( Byung Dong Lee ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.2
To demonstrate the prevalence of Toxocara spp eggs in public playgrounds in Seoul city, sand samples collected from March to November in 2001 were examined. Of 2,600 sand samples from 650 playgrounds surveyed, 41 sands from 39 places(6%) had Toxocara canis eggs. Sand samples in apartment complex were more contaminated(9.7%) with the eggs than in residential area(2.4%). Toxocara canis eggs in sands were found in large number of in the spring but other seasons were less found.
북한산 국립공원 주변지역 사육견의 광견병 항체 분포조사(2)
채희선 ( Hee Sun Chae ),소병재 ( Byung Jae So ),김두환 ( Doo Hwan Kim ),조미영 ( Mee Young Jo ),배니수 ( Nae Soo Bae ),기노준 ( No Joon Ki ),이병동 ( Byung Dong Lee ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.1
Recently, the rabies cases have been reported in Paju-and Yangju-gun, Gyeonggi province near Seoul metropolitan area. The Pukhansan national park, nearly located from the cities, is suspected to be a high risk area for incidence and spread of the rabies to metropolitan area. This study was conducted to investigate the prevalence rate for rabies antibody of dogs near the Pukhansan national park and in some other districts in Seoul metropolitan city. From march to october 2001, a total of 306 serum samples were taken from dogs for breeding(189) and pet dogs(117) in 4 districts near the Pukhansan national park and other districts of Seoul. Rabies virus antibodies in sera were detected by neutralizing peroxidase-linked assay(NPLA). Of the 306 sera of dogs tested, 74(24.2%) were positive to rabies virus antibody. The prevalence rates of rabies antibody in Pukhansan national park area and in the other districts of Seoul city were 23.7% and 25.3%, respectively. There was no significant difference in the prevalence rate between these two districts. The prevalence rates of rabies antibody in pet dogs and dogs for breeding were 40.2% and 14.3% respectively. The prevalence rates of rabies antibodies in less than 1 year, 1~<2 years, 2~<3 years, and over 3 years old dogs were 14.5%, 22.4%, 32.6%, and 27.1%, respectively, and overall 24.2% in the dog population. In addition, we found that dogs less than 1 year old had lower antibody prevalence than those over 1 year old. It was concluded that enhancement of vaccination is important in the prevention of the rabies, and that rabies vaccines should not be less supplied than the population of the dog.
테트라싸이클린계 잔류항생물질 분석을 위한 전처리방법 비교
김동언 ( Dong Eon Kim ),황래홍 ( Lae Hwong Hwang ),윤은선 ( En Sun Yun ),함희진 ( Hee Jin Ham ),양윤모 ( Yoon Mo Yang ),김창기 ( Chang Gi Kim ),기노준 ( No Jun Ki ),이정학 ( Jung Hark Lee ) 한국가축위생학회 2003 韓國家畜衛生學會誌 Vol.26 No.3
Solid phase extraction(SPE) and matrix solid phase dispersion(MSPD) have been studied as preparation procedures for tetracyclines analysis by high-performance liquid chromatography (HPLC) in meat. The recovery range was 74~98% for SPE, and 72~93% for MSPD at spiked levels of l00ng/g for oxytetracycline(OTC), tetracycine(TC), chlortetracycline(CTC), and doxycycline(DC). The detection limits were 15~78ng/g for SPE and 25~84ng/g for MSPD, respectively. Analytical method was HPLC with UV detector. The purpose of this study was developing a practical, accurate and precise method for rapid extraction and quantitation of tetracycline residues in meat.