금화규(Abelmoschus manihot) 꽃 추출물의 In Vitro 피부 미백 및 주름 개선 효능 연구

권현지 ( Hyun-ji Kwon ),범석현 ( Seok-hyun Beom ),현진아 ( Jin-a Hyun ),강은빈 ( Eun-bin Kang ),박하은 ( Ha-eun Park ),한동근 ( Dong-geun Han ),김현정 ( Hyun-jeong Kim ),최은영 ( Eun-young Choi ),안봉전 ( Bong-jeon An ) 대한화장품학회 2021 대한화장품학회지 Vol.47 No.4

본 연구는 화장품 소재로서 금화규(Abelmoschus manihot) 꽃의 미백 활성율 및 항주름 활성을 평가하기 위해 수행하였다. 금화규 꽃은 물과 70% 에탄올을 이용하여 추출하였다. Tyrosinase 억제 활성은 물과 70% 에탄올 추출물 모두 농도 의존적으로 억제 활성이 증가 된다고 평가되었으며, B16F10 세포주에서 생존율에 영향을 미치지 않은 농도인 10, 25, 50, 75, 100 μg/mL에서 멜라닌 생합성을 저해하는 것으로 확인되었다. 금화규 꽃의 항주름 효능으로는 collagenase와 elastase의 활성 저해 능력을 측정한 결과 70% 에탄올 추출물이 물 추출물보다 높은 항주름 활성을 보였다. 본 연구 결과를 통하여 금화규 꽃 추출물은 미백 활성과 항주름 활성을 가지는 기능성 화장품 소재로 사용 가능할 것으로 사료된다. In this study, the skin whitening and anti-wrinkle activity of Abelmoschus manihot (A. manihot) flowers were evaluated as a cosmetic material. A. manihot flowers were extracted using water and 70% ethanol. Tyrosinase inhibitory activity was evaluated to increase concentration-dependent inhibitory activity in both water (AMW) and 70% ethanol extracts (AME), and was found to inhibit melanin biosynthesis at concentrations that did not affect survival in B16F10 cell lines at 10, 25, 50, 75, and 100 μg/mL. As a result of measuring the anti-wrinkle effectiveness of A. manihot flowers, 70% ethanol extract (AME) showed higher anti-wrinkle activity than water extract (AMW). Through the results of this study, it is considered that the A. manihot flower extract can be used as a functional cosmetic material with whitening and anti-wrinkle activity.

Concanavalin A가 코팅 된 자성 입자를 이용한 미생물 농축 및 유전자 추출 칩 개발

권기록 ( Kirok Kwon ),곽호경 ( Hogyeong Gwak ),현경아 ( Kyung-a Hyun ),정효일 ( Hyo-il Jung ) 한국센서학회 2018 센서학회지 Vol.27 No.4

The real-time enrichment and detection of pathogens are serious issues and rapidly evolving field of research because of the ability of these pathogens to cause infectious diseases. In general, bacterial detection is accomplished by conventional colony counting or by polymerase chain reaction (PCR) after DNA extraction. As colony counting requires considerable time to cultivate, PCR is an attractive method for rapid detection. A small number of pathogens can cause diseases. Hence, a pretreatment process, such as enrichment is essential for detecting bacteria in an actual environment. Thus, in this study, we developed a microfluidic chip capable of performing rapid enrichment of bacteria and the extraction of their genes. A lectin, i.e., Concanavalin A (ConA), which shows binding affinity to the surface of most bacteria, was coated on the surface of magnetic particles to nonspecifically capture bacteria. It was subsequently concentrated through magnetic forces in a microfluidic channel. To lyse the captured bacteria, magnetic particles were irradiated by a wavelength of 532nm. The photo-thermal effect on the particles was sufficient for extracting DNA, which was consequently utilized for the identification of bacteria. Our device will help monitor the existence of bacteria in various environmental situations such as water, air, and soil.

AhR 발현을 통한 콤부차 분획물의 안티폴루션 효과 연구

강은빈,현진아,권현지,범석현,한동근,박하은,김현정,곽기성,안봉전,Kang, Eun-Bin,Hyun, Jin-A,Kwon, Hyun-Ji,Beom, Seok-Hyun,Han, Dong-Geun,Park, Ha-Eun,Kim, Hyun-Jeong,Kwak, Ki-Sung,An, Bong-Jeon 대한화장품학회 2021 대한화장품학회지 Vol.47 No.3

본 연구에서는 설탕을 넣은 녹차나 홍차에 유익균을 첨가해 발효시킨 음료인 콤부차를 컬럼 크로마토그래피를 이용해 분획한 후, TLC를 통해 플라보노이드류인 퀘르세틴 글리코사이드(quercetin glycoside)의 유효성분 유무를 확인하고 그 분획물을 이용하여 공해로부터 피부를 보호 및 개선하는 안티폴루션 효과를 확인하였다. 인간 각질형성세포에 Kombucha-quercetin glycoside (K-QG)를 처리하여 세포 생존율을 확인한 결과, 100 ㎍/mL 농도까지 90% 이상의 생존율을 보였으며, 안티폴루션 효과를 보기 위해 benzo[e]pyrene과 미세먼지 자극에 의한 세포 생존율을 측정한 결과, 100 ㎍/mL에서 각각 68.79%, 50.68%의 개선율을 보였다. 또한, benzo[a]pyrene에 의해 활성화되는 aryl hydrocarbon receptor (AhR) 발현을 웨스턴 블롯을 통해 확인한 결과, 대조군에 대비하여 100 ㎍/mL에서 31.08%의 억제율을 보였다. 본 연구의 결과를 통해 K-QG는 벤조피렌과 미세먼지로부터 자극받은 피부를 보호, 개선해주며 안티폴루션 기능성 소재로써 가치가 있는 것으로 사료된다. In this study, Kombucha, a fermented drink by adding beneficial bacteria to green tea or black tea with sugar, was classified using column chromatography, and the presence or absence of quercetin glycoside, a type of flavonoids, was confirmed through TLC. In addition, the anti-pollution effect of protecting and improving the skin from pollution was confirmed using the fraction. Keratinocytes were treated with K-QG to confirm the cell viability, showing a survival rate of 90% or more up to a concentration of 100 ㎍/mL, and the cell viability by benzo[e]pyrene and fine dust stimulation to see the anti-pollution effect was measured. At 100 ㎍/mL, the improvement rates were about 68.79% and 50.68%, respectively. In addition, as a result of confirming the expression of AhR activated by benzo[a]pyrene through Western blot, it showed an inhibition rate of about 31.08% at 100 ㎍/mL compared to the control group. Through the results of this study, K-QG is thought to be valuable as an anti-pollution functional material, protecting and improving skin irritated from benzopyrene and fine dust.

으름 어린잎 식초의 품질특성과 생리활성

권우영(Woo-Young Kwon),이은경(Eun-Kyoung Lee),윤진아(Jin-A Yoon),정강현(Kang-Hyun Chung),이권재(Kwon-Jai Lee),송병춘(Byeong Chun Song),안정희(Jeung Hee An) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.7

We investigated the characteristics and biological activities of vinegars added with different levels (0%, 0.5%, 1%, 2%, and 3%) of young leaves of Akebia quinata. During alcohol fermentation, alcohol and total acidity contents of vinegars increased. During acid fermentation, total acidity and amino acid contents increased. Vinegar added with 3% A. quinata leaf showed the highest total sensory score. The contents of total polyphenols, flavonoids, and tannin significantly increased during fermentation according to the amount of A. quinata leaf. After 22 days of fermentation, total polyphenol, total flavonoid, and tannin contents of vinegar added with 3% A. quinata were 4,079.08 mg GAE/100 g, 2,927.08 mg CE/100 g, and 3,618.00 mg TAE/100 g, respectively. ABTS radical scavenging activity of vinegar added with 3% A. quinata was 79.63%. Anti-cancer activities of vinegar added with 3% A. quinata were 48.65% and 52.90% against MCF-7 and HepG2 cells, respectively. Vinegar added with 3% A. quinata showed anti-bacterial activities against Bacillus cereus, Shigella flexneri, Salmonella enterica, Bacillus subtilis, and Klebsiella pneumoniae. Our results demonstrate that the biological activities of vinegar added with 3% A. quinata leaf (22 days of fermentation) were excellent, and their enhanced total polyphenol, flavonoid, and tannin contents were associated with antioxidant, anti-cancer and anti-microbial activities. Thus, A. quinata can be used as a functional material in vinegar and other foods.

찔레꽃(Rosa multiflora) 추출물과 Ascorbic Acid의 시너지 효과를 통한 항산화 활성과 총 페놀함량 및 플라보노이드 함량 분석

범석현 ( Seok-hyun Beom ),권현지 ( Hyun-ji Kwon ),현진아 ( Jin-a Hyun ),강은빈 ( Eun-bin Kang ),박하은 ( Ha-eun Park ),한동근 ( Dong-geun Han ),김현정 ( Hyun-jeong Kim ),최은영 ( Eun-young Choi ),안봉전 ( Bong-jeon An ) 대한화장품학회 2021 대한화장품학회지 Vol.47 No.4

본 연구에서 찔레꽃을 추출, 농축 및 동결 등을 통하여 시료화 하였다. 찔레꽃추출물과 ascorbic acid의 복합물과 단일 ascorbic acid의 항산화 효능을 비교하였다. 본 연구의 목적은 찔레꽃추출물과 ascorbic acid의 시너지 효과를 통한 항산화 효능을 분석하는 것이었다. 실험은 전자공여능, ABTS⁺radical 소거능, SOD 유사 활성, xanthine oxidase 저해 활성, 환원력, FRAP, 폴리페놀 함량 및 플라보노이드 함량을 측정하였다. 그 결과, 전자공여능 실험과 xanthine oxidase 저해 활성 실험에서 복합물이 대조군(ascorbic acid)보다 높은 항산화 활성을 보였다. 플라보노이드 함량 분석 실험의 경우, 100 μg/mL의 농도에서 RMW+A (272.1 mg QE/g), RME+A (90.6 mg QE/g), RMW+A (79.4 mg QE/g)이 대조군(19.0 mg QE/g)보다 함량이 더 높았다. 따라서 찔레꽃추출물과 ascorbic acid 복합물은 단독 사용한 경우에 비하여 시너지 효과를 발휘했다. 따라서 이 복합물은 항산화 화장품에 사용할 경우 제품의 안정화 및 피부노화 억제에 기여할 것으로 사료된다. In this study, Rosa multiflora was sampled through extraction, concentration, and freezing. The antioxidant efficacy of a mixture of R. multiflora extract and ascorbic acid was compared with a single ascorbic acid. The purpose of this study was to analyze the antioxidant efficacy through the synergistic effect of R. multiflora extract and ascorbic acid. The experiment measured electron donating ability, ABTS⁺ radical scavenging, SOD-like activity, xanthine oxidase inhibition activity, reducing power, FRAP, polyphenolic content measurement, and flavnoid content measurement. As a result, the complex showed higher antioxidant activity than the control group (ascorbic acid) in the electron donating ability test and the xanthine oxidase inhibitory activity test. In the case of the flavonoid content experiment, it was confirmed that RMW+A (272.1 mg QE/g), RME+A (90.6 mg QE/g), and RMW+A (79.4 mg QE/g) at a concentration of 100 μg/mL had a higher flavonoid content than the control group (19.0 mg QE/g). Therefore, the extract and ascorbic acid complex exhibited a synergistic effect compared to the single use. Therefore, it is thought that this complex will contribute to product stabilization and skin aging inhibition when used in antioxidant cosmetics.

으름 열매 식초의 품질특성 및 항산화 활성

이은경(Eun-Kyoung Lee),권우영(Woo-Young Kwon),이지원(Ji-Won Lee),윤진아(Jin-A Yoon),정강현(Kang-Hyun Chung),송병춘(Byeong Chun Song),안정희(Jeung Hee An) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.8

본 연구에서는 으름 열매를 각각 0, 1, 3, 5 및 7%를 첨가하여 2단계 발효한 으름 열매 식초의 발효 중 이화학적 특성과 항산화 활성에 대하여 조사하였다. 식초의 이화학적 특성은 pH, 총산, 알코올, 총당, 아미노산으로 평가하였고, 항산화 활성은 ABTS?+ 라디칼 소거능, SOD 유사활성능, 환원력으로 평가하였다. 알코올 발효 중 총산과 알코올 함량은 증가하였고 총당 함량은 감소하였다. 초산 발효에서는 총산 함량이 유의적으로 증가하였다. 관능검사 평가에서는 으름 열매7% 첨가군에서 종합적 기호도가 가장 높게 나타났다. 발효1일째보다 발효 13일째에 대조군과 으름 열매 첨가 1%에서는 총 폴리페놀 함량에 유의적 차이가 없었으나 으름 열매첨가군 3, 5, 7%에서 총 폴리페놀 함량이 각각 136.6, 381.59, 415.35 mg/100 g으로 유의적으로 증가됨을 볼 수 있었다. 총 플라보노이드 함량은 발효 1일째보다 발효 13일째에 대조군이 21.73 mg/100 g, 으름 열매 첨가군 1, 3, 5, 7%에서 각각 15.79, 15.15, 26.19, 26.87 mg/100 g으로 발효가 진행됨에 따라 유의적으로 증가하였고 으름 열매 첨가량에 따라서도 유의적으로 증가되었다. 탄닌의 함량도 발효 1일째보다 발효 13일째에 대조군이 0.2042 mg/100 g, 으름 열매 첨가군 1, 3, 5, 7%에서 각각 0.2004, 0.1255, 0.1384, 0.1255 mg/100 g으로 유의적으로 증가됨을 보였다. ABTS?+ 라디칼 소거 활성은 발효 1일째보다 발효 13일 째에 5 mg/mL 농도에서 대조군이 5.87%, 으름 열매 첨가군1, 3, 5, 7%에서 각각 12.59, 25.63, 34.02, 35.25%로 라디칼 소거능이 증가하였고, SOD 유사활성능도 5 mg/mL 농도에서 발효 1일째보다 발효 13일째에 대조군이 8.22%, 으름열매 첨가군 1, 3, 5, 7%에서 각각 17.49, 16.86, 16.89, 15.68% 증가하였다. 환원력은 으름 열매 첨가군 7%에서 발효 1일째에 0.527, 발효 13일째에 1.539로 나타났다. 본 연구 결과에서 항산화 활성은 발효가 진행되는 동안 증가하였고 으름 열매 첨가량에 따라서도 증가하였다. 으름 열매식초가 발효됨에 따라 ABTS?+ 라디칼 소거능, SOD 유사활성능, 환원력이 증가하였으며 이것은 높은 폴리페놀, 플라보노이드, 탄닌의 함량이 밀접하게 관여한 것으로 보인다. 그러므로 으름 열매의 높은 항산화 활성이 식초 및 기타 식품에서 기능성 소재로 이용될 것으로 보여준다. This study investigated the physicochemical properties and antioxidant activity of vinegar added with different levels (0%, 1%, 3%, 5%, and 7%) of Akebia quinata fruit during two-step fermentation. The physicochemical properties of vinegar evaluated were pH, total acidity, alcohol, and total sugar and amino acid contents. The antioxidant activities were based on ABTS radical scavenging activity, SOD-like activity, and reducing power. During alcohol fermentation, total acidity and alcohol contents of vinegar increased, but total sugar contents decreased. During acid fermentation, total acidities of vinegar increased. Vinegar added with 7% A. quinata fruit showed the highest total sensory score. Total polyphenol contents of vinegar added with 0% and 1% A. quinata fruit were not significantly different. However, vinegar added with 3, 5, and 7% A. quinata fruit showed significantly higher total polyphenol contents of 136.6, 381.59, and 415.35 mg/100 g, respectively, after 13 days of fermentation. Further, total flavonoid contents of vinegar added with 0~7% A. quinata fruit significantly increased to 21.73, 15.79, 15.15, 26.19, and 26.87 mg/100 g, respectively, after 13 days of fermentation. In addition, tannin contents of vinegar added with 0~7% A. quinata fruit significantly increased to 0.2042, 0.2004, 0.1255, 0.1384, and 0.1255 mg/100 g, respectively, after 13 days of fermentation. Moreover, ABTS radical scavenging activities of vinegar added with 0~7% A. quinata fruit significantly increased to 5.87, 12.59, 25.63, 34.02, and 35.25, respectively, after 13 days of fermentation at a concentration of 5 mg/mL. Additionally, SOD-like activities of vinegar added with 0~7% A. quinata fruit significantly increased to 8.22, 17.49, 16.86, 16.89, and 15.68%, respectively, after 13 days of fermentation. Reducing power of 7% A. quinata fruit was 0.527 after 1 day and 1.539 at the end of fermentation. Our results demonstrate that antioxidant activity significantly increased during fermentation according to the content of A. quinata. Further, the total polyphenol, flavonoid, and tannin contents were shown to be closely related with antioxidant activities. Thus, A. quinata could be effectively used as a vinegar and functional food material based on its antioxidant activity.

다중 오리피스 유동분획 채널을 병렬로 연결한 고효율 순환종양세포 분리기

현경아(Kyung-A Hyun),권기호(Kiho Kwon),김승일(Seung-Il Kim),한현주(Hyunju Han),정효일(Hyo-Il Jung) 대한기계학회 2012 대한기계학회 춘추학술대회 Vol.2012 No.11

금 나노 입자의 광열 효과를 이용한 공기 중 미생물 농축 및 용해 칩

권기록(K. Kwon),현경아(K.A. Hyun),정효일(H.I Jung) 한국생산제조학회 2015 한국생산제조시스템학회 학술발표대회 논문집 Vol.2015 No.4

찔레꽃(Rosa multiflora) 추출물의 주름개선 및 열노화 저해 활성

현진아(Jin A Hyun),강은빈(Eun Bin Kang),김영석(Young Seok Kim),이효석(Hyo Seok Lee),권현지(Hyun Ji Kwon),범석현(Seok Hyun Beom),최은영(Eun Young Choi),박가은(Ga Eun Park),장민정(Min Jung Jang),안봉전(Bong Jeun An) 한국식품저장유통학회 2021 한국식품저장유통학회지 Vol.28 No.6

재협착 방지 혈관스탠트 제작을 위한 혈관세포 거동 억제 마이크로스케일 표면형상 패터닝

이혜림(Hye Lim Lee),권청아(Chung-A Kwon),박재호(Jae Ho Park),최유미(Yu-Mi Choi),김유찬(Yu-Chan Kim),석현광(Hyun-Kwang Seok),남구현(Koohyun Nam),한준현(Jun Hyun Han),전호정(Hojeong Jeon) 대한기계학회 2013 대한기계학회 춘추학술대회 Vol.2013 No.12