전자레인지 조리에 의한 적양배추의 항산화력 및 대장암세포 증식억제

권태은,정하숙,Guon, Tae-Eun,Chung, Ha Sook 한국식품조리과학회 2015 한국식품조리과학회지 Vol.31 No.1

The present study was performed to investigate antioxidant and anti-colon cancer activities of red cabbage (Brassica oleracea L. var. capitata f. rubra DC) according to the cooking conditions (raw, microwave, blanching and steaming). The contents of red cabbage extracts were determined as follow: total phenolic contents, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethy lbenzo-thiazoline-6-sulfonic acid) (ABTS), 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay, western blot analysis. The total contents of polyphenol and flavonoid of red cabbage were 20.27 mg GAE/g Dry weight ${\pm}0.03$ and $2.55{\pm}0.02mg$ RE/g Dry weight. In this study, the total contents of polyphenol were decreased to both microwave and steam cooking. Total antioxidant activity and growth inhibition of HCT116 human colon cancer cells were in the order of raw > microwaving > steaming cooking methods. These results indicate that red cabbage extracts might have antioxidant and anti-proliferative activity according to the cooking conditions.

녹차(대작)를 첨가한 기능성 젤리의 품질 특성과 항산화 효능

권태은(Tae Eun Guon),정지승(Ji Seung Jeong),정하숙(Ha Sook Chung) 한국차학회 2020 한국차학회지 Vol.26 No.2

본 연구는 건강효능이 우수한 대작의 소비촉진을 목적으로 기능성 젤리를 제조하여 품질평가를 시행하였다. 실험 결과, 대작의 첨가비율이 20, 40, 60%로 증가할수록 젤리의 pH가 5.89, 5.58과 5.35로 감소하였다(p<0.05). 젤리의 색도를 측정한 결과, L 값도 동일하게 43.31, 40.56과 39.88로 유의적으로 감소하였다(p<0.05). 또한, 적색도(redness)를 나타내는 a값과 황색도(yellowness)를 나타내는 b값은 각각 -0.11, -0.05과 -0.01 및 2.77, 3.08과 3.42로 유의적으로 증가하였다(p<0.05). 젤리 L값의 감소는 찻잎에 함유된 당과 아미노산이 제조과정 중고온처리(hot-air drying)로 인한 비효소적 갈변(Maillard reaction)에 의한 것으로 판단되며, a값과 b값의 증가는찻잎의 폴리페놀 화합물에 의한 산화적 갈변반응에 의한 것으로 판단된다. 젤리의 경도는 0.93, 0.84, 0.79 N으로감소하였으며, 이는 젤리의 유기산이 gel 단백질 분자의 결합을 저해하여 강도를 감소시킨 것으로 판단된다. 대작 젤리의 총 페놀성 화합물 함량은 각각 16.98, 26.75, 32.17 mg GAE/g으로 유의적으로 증가하였으며(p<0.05), DPPH free radical 및 ABTS radical 소거능은 각각 64.84%, 75.46%, 79.85% 및 19.01%, 39.01%, 54.59%로 유의적으로 증가하였다(p<0.05). 이번 실험결과는 소화가 용이하고 항산화 효능이 우수한 기능성 젤리 소재개발에 기여할 수 있을 것으로 판단된다. This study examined the quality characteristics and antioxidant properties of jellies with Daejak. Daejak jellies were prepared with 0, 20, 40 and 60% of brewed Daejak (1 g/100 mL hot water), sugar, and gelatin. As a result, the pH of brewed Daejak jellies contents of 20, 40, and 60% were 5.89, 5.58, and 5.35, respectively (p<0.05). The L-value and hardness decreased significantly with increasing levels of brewed Daejak (p<0.05), but the a-value and b-value increased significantly with increasing levels of brewed Daejak in jellies (p<0.05). The total phenolic compounds and DPPH, ABTS activities increased with increasing concentration of brewed Daejak (p<0.05). Overall, Daejak can be useful in the production of high-quality jelly.

대용차 소재 아프리카 약용식물의 항산화 및 HCT116 인체 대장암세포 성장저해 효과

권태은(Tae-Eun Guon),윤경미(Kyung-Mi Yoon),정하숙(Ha Sook Chung) 한국차학회 2015 한국차학회지 Vol.21 No.3

본 연구에서는 천연에 존재하는 약용작물을 활용한 대용 차 소재 발굴을 목적으로, 아프리카의 대표적 약용작물인 K. africana 및 M. oleifera 잎 추출물의 항산화 효과 및 인체 대장암 세포주인 HCT116 증식억제에 미치는 효과를 규명 하였다. 실험 결과, 건조된 시료 추출물의 총 페놀성 화합물 함량은 K. africana의 경우 1.308 mg/g, M. oleifera는 1.241 mg/g으로 나타났다. DPPH radical scavenging 효과는 K. africana 잎 추출물의 경우, 0.1 mg/mL 농도에서 83.22%, 0.2 mg/mL에서 99.78%로 나타났으며, M. oleifera는 0.1mg/mL 농도에서 40.82%, 0.2 mg/mL와 0.8 mg/mL에서는 각각 65.12%와 88.48%로 나타나 시료 농도와 비례하여 유 의적으로 증가하였다. 또한 인체 대장암 세포주인 HCT116 에 K. africana 및 M. oleifera 잎 추출물을 농도별로 처리하 였을 때, 성장저해가 유의적(p < 0.05)으로 증가하였으며, 특히 M. oleifera 잎 추출물이 성장저해 효과가 높은 것으로 확인되었다. 이어서 HCT116 세포 생존을 조절하는 것으로 알려진 P53과 Bcl-2 family 발현에 미치는 K. africana 및 M.oleifera 효과를 관찰한 결과, 암 억제 유전자인 p53과 Bax의 발현은 증가하였고, 반대로 Bcl-2의 발현은 감소하여, K.africana 및 M. oleifera 잎 추출물이 HCT116 세포사멸에 중요한 역할을 하는 것으로 관찰되었다. 이상의 실험결과를 통해 K. africana 및 M. oleifera 잎 추출물이 항산화 및 HCT116 세포사멸에 효과가 있음이 확인되었으며, 이를 활용한 대용차 개발 및 건강기능 소재 발굴을 위한 기초자료 로 활용될 수 있으리라 기대된다. The present study was performed to investigate the antioxidant and anti-proliferative activities of African traditional plants, Kigelia africana and Moringa oleifera leaves, on HCT116 human colon cancer cells. This study examined the antioxidants and apoptotic analysis with the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging, 3-[4,5-dimethylthiazol- 2-yl]-2,5-diphenyltetrazolium bromide assay and western blotting experiments. The total phenolic contents of the 70% ethanolic extracts of K. africana and M. oleifera leaves were 1.308±0.0071 mg GAE/g dry weight and 1.241±0.9205 mg GAE/g dry weight, respectively. The effects of K. africana and M. oleifera leaves on the apoptotic pathway were also investigated. The Bcl-2 level decreased, but the Bax and p53 levels increased in a dose-dependent manner. These results suggest that K. africana and M. oleifera leaves might be promising candidates for natural ingredients to produce healthy food and alternative tea resources.

보이차(Pu-erh tea)의 HaCaT 세포보호 및 HCT116 세포사멸 효과

권태은(Tae Eun Guon),정지승(Ji Seung Jeong),정하숙(Ha Sook Chung) 한국차학회 2016 한국차학회지 Vol.22 No.4

The present study was performed to investigate the biological activity of fermented Pu-erh (FP) and raw Pu-erh (RP) tea extractives. In this study, we examined the antioxidants with the DPPH and ABTS assays and conducted an apoptotic analysis with the MTT assay and western blotting experiments. The total phenolic contents in FP and RP were 38.59 ± 15.23 and 36.83 ± 6.60 mg GAE/g dry weight, respectively. The effects of FP and RP on the apoptotic pathway were also investigated. It was found that the levels of Bcl-2 decreased, whereas those of Bax and cleaved caspase-9, caspase-3 and PARP increased in a dose-dependent manner. In addition, we investigated the protective effects of FP and RP against ultraviolet B-induced oxidative damage in human keratinocytes (HaCaT cells). To induce oxidative stress in the HaCaT cells, they were irradiated with UVB at 30 mJ/㎠. FP and RP was found to be reduced phosphorylation of IkBα and IKKα/β, block nuclear factor-kB p65 nuclear translocation, and strongly suppress the pro-inflammatory mediators.

우전 추출물의 항산화 및 HCT116 인체대장암 세포사멸 효과

권태은(Tae Eun Guon),정지승(Ji Seung Jeong),정하숙(Ha Sook Chung) 한국차학회 2017 한국차학회지 Vol.23 No.3

Woojeon is a green tea plucked in early to mid-April, which has a beautiful, smooth and concentrated flavor that lingers in the mouth and evaporates on the mid-tongue. Polyphenols, the main components of green tea, are antioxidant phytochemicals and are known to have protective effects against various diseases. The objective of this study is to investigate the antioxidant and anticancer activities of Woojeon on HCT116 human colon cancer cell lines. It was found that the total polyphenol contents of Woojeon was 30.09 mg GAE/g, and antioxidant activities were 73.85%, 33.26% and 48.52 mg TEAC/g, measured by DPPH, ABTS and FRAP assay, respectively. The treatment of HCT116 human colon cancer cell lines with Woojeon at various concentrations resulted in a sequence of changed characteristic of apoptosis, including the loss of cell viability and morphological changes. Further, annexin V-fluorescein isothiocyanate and propidium iodide double staining through flow cytometry analysis demonstrated that the degree of apoptosis was increased by Woojeon. In addition, the Bax and Bcl-2 expression levels were increased in a dose-dependent manner, resulting in the activation of caspase-9, caspase-3 and cleaved poly-ADP-ribose polymerase; these observations are hallmarks of apoptotic events. Moreover, c-Jun N-terminal kinase, p38 MAPK and extracellular signal-regulated kinase was indicated Woojeon-induced apoptosis, and was accompanied by phosphorylation of the mitogen-activated protein kinase family.

생강(Zingiber officinale Roscoe) 추출물의 항산화 및 A2058 흑색종세포 사멸 효과

권태은(Tae-Eun Guon),정하숙(Ha Sook Chung) 동아시아식생활학회 2016 동아시아식생활학회지 Vol.26 No.3

강인한 사운드통신을 위한 딥러닝 기반 수신기 설계

임선홍,최준원 한국통신학회 2021 한국통신학회 학술대회논문집 Vol.2021 No.6

본 논문은 비가청 대역의 음파신호를 이용하여 통신을 수행하는 음파통신에서 딥러닝을 기반한 수신기를 제안하였다. 사운드 통신에서는 전파통신에 비해 간단한 구조를 가지고 있기 때문에 상대적으로 부정확한 채널추정 성능으로 인해 성능열화가 일어난다. 본 논문에서는 부정확한 채널추정 환경에서 딥러닝 모델을 이용하여 이를 보완할 수 있는 방법을 제안하였다.

불발효차 우전, 세작, 중작, 대작의 HCT116 세포사멸 효능

권태은(Tae Eun Guon),정하숙(Ha Sook Chung) 한국차학회 2018 한국차학회지 Vol.24 No.1

In this present study, we investigated the anticancer activities of non-fermented teas (Woojeon, Sejak, Jungjak and Daejak) in HCT116 human colon cancer cells. The antioxidant activities of Woojeon, Sejak, Jungjak and Daejak at 0.1 mg/mL in terms of their DPPH free radical scavenging activities were found to be 91.2, 91.6, 92.7 and 94.4%, respectively. The treatment of HCT116 human colon cancer cells with 0.6 mg/mL of Woojeon, Sejak, Jungjak and Daejak resulted in the characteristic sequence of apoptosis and morphological changes. The cell viability was also decreased upon the addition of Woojeon, Sejak, Jungjak and Daejak, and the main catechins, (-)-epicatechingallate, (-)-epigallocatechingallate and (-)-epigallocatechin, to HCT116 human colon cancer cells. Moreover, the Bax and Bcl-2 expression levels were increased, resulting in the activation of caspase-9, caspase-3 and cleaved poly-ADP-ribose polymerase; these observations are hallmarks of apoptotic events.

연근(Nelumbo nucifera)의 HT-29 세포 사멸 효과

권태은(Tae Eun Guon),류민주(Min Ju Ryu),서진아(Jin A Seo),정하숙(Ha Sook Chung) 한국약용작물학회 2012 한국약용작물학회 학술대회논문집 Vol.2012 No.2

기능성 비빔밥 재료의 HT-29 세포 사멸 효과

권태은(Tae Eun Guon),서진아(Jin A Seo),류민주(Min Ju Ryu),탁상숙(Sang Sook Tak),정하숙(Ha Sook Chung) 한국약용작물학회 2012 한국약용작물학회 학술대회논문집 Vol.2012 No.2