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Efficacy of Gene Transfer and Expression of Novel Recombinant Baculovirus Vector
권태동,홍성갑,Kweon, Tae-Dong,Hong, Seong-Karp The Korea Institute of Information and Commucation 2014 한국정보통신학회논문지 Vol.18 No.8
폴리히드론 프로모터, 수포성구내염 바이러스G, 폴리A, 사이토메가바이러스 프로모터, 강화녹색형광단백질, 단백질전달부위 유전자 등을 포함한 새로운 베큘로바이러스 시스템이 제조되었다. 본 재조합벡터 시스템은 인간 섬유아세포에 적용하여 시험하였고 재조합된 유전자의 전달과 유전자 발현을 대조 벡터시스템과 비교하였다. 본 연구로부터 새롭게 제작된 본 베큘로바이러스 시스템이 유전자의 전이와 유전자 발현 면에서 대조 벡터시스템 보다 고효율을 나타내었다. Novel baculovirus vector systems recombined with coding genes of polyhedron promoter, vesicular stomatitis virus G (VSVG), polyA, cytomegalovirus (CMV) promoter, enhanced green fluorescent protein (EGFP), and protein transduction domain (PTD) were constructed. These recombinant baculovirus vector systems were applied into human foreskin fibroblast cells and compared the effects of gene transfer and gene expression of these recombinant baculovirus vector systems with control vector system. From this study, it showed that these novel recombinant baculovirus vector systems were superior efficacy to control vector system in view of gene transfer and gene expression.
교감신경 의존적 및 비의존적 신경병증 통증 쥐 모델 후근신경절에서 Ca<sup>++</sup> Channel α2δ subunit와 TRPM8 발현
한동우,권태동,김연아,최종범,이윤우,Han, Dong Woo,Kweon, Tae Dong,Kim, Yeon A,Choi, Jong Bum,Lee, Youn Woo 대한통증학회 2008 The Korean Journal of Pain Vol.21 No.1
Background: Peripheral nerve injury induces up-regulation of the calcium channel alpha2delta (${\alpha}2{\delta}$) subunit and TRPM8 in the dorsal root ganglion (DRG) which might contribute to allodynia development. We investigated the expression of the ${\alpha}2{\delta}$ subunit and TRPM8 in the DRG of sympathetically maintained pain (SMP) and sympathetic independent pain (SIP) rat model. Methods: For the SMP model, the L5 and L6 spinal nerves were ligated tightly distal to the DRG. For the SIP model, the tibial and sural nerves were transected, while the common peroneal nerve was spared. After a 7 day postoperative period, tactile and cold allodynia were assessed using von Frey filaments and acetone drops, respectively. Expression of the ${\alpha}2{\delta}$ subunit and TRPM8 in the L5 and L6 DRG were subsequently examined by a Western blot. Results: There were no significant differences between the two models for the thresholds of tactile and cold allodynia. Expression of the ${\alpha}2{\delta}$ subunit in the ipsilateral DRG to the injury was increased as determined on a Western blot as compared to that in the contralateral or sham-operated DRG of the SMP model, but there was no difference in expression seen with the use of the SIP model. There was no difference in the expression of TRPM8 in the ipsilateral DRG to the injury and the contralateral or sham-operated DRG of either model. Conclusions: Up-regulation of the ${\alpha}2{\delta}$ subunit in injured DRG may play a role that contributes to tactile allodynia development in SMP, but not TRPM8 to cold allodynia after peripheral nerve injury.
약제내성 Mycobacterium tuberculosis의 rpoB 유전자 분석과 클로닝 발현
최은경,권태동,배선준,조해선,홍성갑,Choi, Eun Kyeong,Kweon, Tae-Dong,Bai, Sun-Joon,Cho, Hae Sun,Hong, Seong-Karp 한국정보통신학회 2013 한국정보통신학회논문지 Vol.17 No.4
마산병원과 결핵연구원에서 rifampin 내성균주를 확보하여 기존의 전통방법으로 검사된 항결핵제 내성균의 rifampin 내성관련 유전자인 rpoB (RNA polymerase beta subunit)의 변이를 DNA 염기서열 분석방법에 의하여 분석하였다. 그 결과 국내에서 분리되는 결핵균에서는 기존에 보고된 rpoB 변이부위와는 다른 위치의 DNA 염기 변이가 확인되었고 국내에서 여러 내성 결핵균주에서 변이가 발견되고 있지만 이러한 변이가 리팜핀 내성을 실제로 유발하는지 실험적으로 검증된 바는 없었다. 따라서 이러한 특이 부위의 rpoB변이들이 실제로 리팜핀 내성을 유발하는가를 확인하기 위하여 내성 결핵균주들의 rpoB 변이유전자를 polymerase chain reaction(PCR)으로 증폭하고 이것을 리팜핀 감수성 결핵균주에 cloning(클로닝)하고 발현시켜 rpoB 변이가 리팜핀에 대한 내성을 발생시켰음을 실험적으로 확인하였다. Using DNA sequencing method, we analyzed mutations of rpoB (RNA polymerase beta subunit) rifampin-resistant Mycobaterium tuberculosis strains which were identified by conventional test at Masan National Hospital and The Korean Institute of Tuberculosis. Though it has been reported different mutations of rpoB region of rifampin-resistant M. tuberculosis strains in the south of Korea, it is not confirmed whether these mutations of rpoB region actually express rifampin resistance through experiment. We confirmed experimentally these mutations of rpoB region of M. tuberculosis strains induced rifampin-resistance through ampified rpoB by polymerase chain reaction (PCR) and cloning of mutant rpoB into rifampin sensitive-M. tuberculosis strain.
실험연구 : 흰쥐의 포르말린 검사에서 척수강 내로 혼합 투여한 NMDA 수용체 길항제와 5-HT3 수용체 작동제의 상호 작용
한동우 ( Dong Woo Han ),권태동 ( Tae Dong Kweon ),이종석 ( Jong Seok Lee ),유영철 ( Young Chul Yoo ),이윤우 ( Youn Woo Lee ),김선종 ( Sun Chong Kim ) 대한마취과학회 2007 Korean Journal of Anesthesiology Vol.52 No.6
Background: A single agent administered is not effective enough to block nociception. Combination of agents acting through different mechanisms may be one of the best ways for better analgesic methods. The purpose of this study was to investigate the spinally mediated analgesic interaction between 5-HT3 receptor agonist (m-CPBG) and NMDA receptor antagonist (MK801) in the rat formalin test. Methods: A polyethylene catheter was inserted in the atlanto-occipital membrane to the thoracolumbar level of the spinal cord. On postoperative 7 day, the normal saline (n = 6), m-CPBG (10, 30, 100μg: n = 18) and MK801 (0.1, 1, 10μg: n = 18) were injected intrathecally before subcutaneous injection of 5% formalin. The combinations of each 1/2ED50 + 1/2ED50, 1/4ED50 + 1/4ED50, 1/8ED50 + 1/8ED50 were tested and the ED50 of the combination was determined. The experimental ED50 values were compared with the theoretical additive values. Results: m-CPBG dose-dependently decreased the number of flinches in both phase 1 and 2. MK801 dose-dependently reduced the number of flinches in phase 2, but not in phase 1. The combination of m-CPBG and MK801 produced dose-dependent decreases of flinches on both phase 1 and 2. ED50 values (2.75μg for m-CPBG and 0.076μg for MK801) of phase 2 in the combination were significantly less than the calculated additive values (20.0μg for m-CPBG and 0.55μg for MK801)(P<0.01). Conclusions: Intrathecally administered m-CPBG and MK801 had synergistic analgesic effects on formalin-induced hyperalgesia as well as antinociceptive effects in phase 1 on the rat formalin test. (Korean J Anesthesiol 2007; 52: 694~701)
실험연구 : 흰쥐 포르말린 검사에서 지주막하강 내 Pregabalin의 전처치와 후처치에 따른 진통효과 비교
조남룡 ( Nam Ryong Cho ),권태동 ( Tae Dong Kweon ),한동우 ( Dong Woo Han ),윤경봉 ( Kyung Bong Yoon ),이윤우 ( Youn Woo Lee ) 대한마취과학회 2007 Korean Journal of Anesthesiology Vol.53 No.4
Background: Pregabalin is an analog of gamma aminobutyric acid, and selectively interacts with the alpha-2-delta subunit of the voltage dependent calcium channels. The aims of this study were to investigate the analgesic effects of intrathecal pregabalin in rat formalin tests and to compare between the pre-treatment and post-treatment group. Methods: All experimental animals were randomly divided into pre-and post-treatment groups. In pre-treatment groups, pregabalin (0.003μg, 0.01μg, 0.03μg, 0.1μg, n = 6 at each group) was administered through the intrathecal catheter 10 min prior to formalin injection. In post-treatment groups, pregabalin (0.01μg, 0.03μg, 0.1μg, 0.3μg, n = 6 at each group) was administered through the catheter 10 min after formalin injection. Formalin (50 ml, 5%) was injected in the left hind paw. We counted the number of flinching as a pain behavior for 60 min to quantify the nociceptive response. Results: The withdrawal responses which were represented by flinching count, were decreased dose dependently in the phase 2, in all groups (pre-treatment and post-treatment group), while there were less analgesic effects and ceiling effects in the phase 1. There was more significant decreasing flinching number in the pre-treatment group than that in the post-treatment group. Conclusions: Intrathecal pregabalin has preemptive analgesic effect and may be useful in the management of inflammation induced hyperalgesia. (Korean J Anesthesiol 2007; 53: 491~6)
임상연구 : 우심실기능평가에 있어서 경식도심초음파와 열희석법의 비교
나성원 ( Sung Won Na ),권태동 ( Tae Dong Kweon ),오영준 ( Young Jun Oh ),홍용우 ( Yong Woo Hong ),김지호 ( Ji Ho Kim ),곽영란 ( Young Lan Kwak ) 대한마취과학회 2006 Korean Journal of Anesthesiology Vol.51 No.1
Background: It is important to evaluate the right ventricular (RV) function intraoperatively for optimal management of patients undergoing cardiac surgery. The clinical relevance of thermodilution method to tranesophageal echocardiography (TEE) for the measurements of RV function during off-pump coronary artery bypass surgery (OPCAB) was evaluated in this study. Methods: Fifty patients undergoing OPCAB were included. RV function was assessed with both thermodilution method and TEE after anesthesia induction (T1), 10 min after leg elevation (T2), 10 min after returning to the supine position (T3), 10 min after Y-graft formation started (T4), 10 min after sternum closure (T5) and 10 min after applying 5 cmH2O of positive end expiratory pressure (T6). Fractional area change (FAC) and tricuspid annular plane systolic excursion ratio (TAPSE) were measured by TEE and compared to RV ejection fraction (RVEF) measured by thermodilution technique. RV end diastolic area (EDA) derived from TEE was compared to RV end diastolic volume index (EDVI) derived from thermodilution technique. Results: There was no significant correlation between echocardiographic data and thermodilution data by linear regression analysis. However, only in patients with right coronary artery stenosis less than 90%, TAPSE and FAC were significantly correlated with RVEF (P = 0.008 and 0.01, respectively) and EDA was significantly correlated with EDVI (P = 0.013) only at T4. Conclusions: RV performance measured by thermodilution technique did not correlate well with TEE finding in severe coronary artery disease patients. The correlation was significant during hemodynamically stable period (during Y-graft formation) in patients without severe right coronary artery disease. (Korean J Anesthesiol 2006; 51: 58~64)