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Isolation of porcine epidemic diarrhea virus (PEDV) in Korea
권창희,권병준,정태성,기영진,허동호,황의경,이재진,안수환,Kweon, Chang-hee,Kwon, Byung-joon,Jung, Tae-sung,Kee, Young-jin,Hur, Dong-ho,Hwang, Eui-kyung,Rhee, Jae-chin,An, Soo-hwan The Korean Society of Veterinary Science 1993 大韓獸醫學會誌 Vol.33 No.2
설사로 폐사한 자돈의 장 절편을 이용하여 돼지 유행성 설사바이러스(PEDV), 돼지 전염성 위장염 바이러스(TGEV), 돼지 로타 바이러스(PRV)에 대한 병인학적 검사를 형광항체반응을 통하여 조사하였던 바 돼지 유행성 설사 바이러스의 감염에 의한 자돈의 폐사를 확인하였다. 간접형광항체검사시 돼지 유행성 설사 바이러스에 대한 양성반응을 보인 가검재료를 이용 Vero세포에 연속 계대한 후 plaque assay를 통하여 크로닝된 돼지 유행성 설사 바이러스 KPEDV-9주를 작성하였다. 돼지 유행성 설사 바이러스에 대한 면역혈청과 바이러스가 분리된 농장에서 채취된 돼지 혈청을 이용 돼지 유행성 설사 바이러스에 대한 구조단백성분을 분석하였던 바 88K(M.W.), 74K, 70K, 58~54K, 54~46K, 44~40K 및 33~32K에 상당하는 단백성분을 검출할 수 있었다. The etiological survey on porcine epidemic diarrhea virus(PEDV) by immunofluorescence antibody test(IFA) showed the positive rusult from the intestines of piglet died from acute diarrhea. The viral agent of PED was also isolated from intestine, which showed positive reaction by immunofluorescence test. After passage in Vero cell, the viral agent was further cloned by plaque purification and designated as KPEDV-9. The immunoblotting analysis using hyperimmune sera and porcine sera revealed the presence of several polypetide bands with molecular weight(M.W.) of 88K, 74K, 70K, 58~54, 54~46K, 44~40K and 33~32K, respectively.
권창희,권병준,강영배,안수환,Kweon, Chang-hee,Kwon, Byung-joon,Kang, Yung-bai,An, Soo-hwan The Korean Society of Veterinary Science 1994 大韓獸醫學會誌 Vol.34 No.2
돼지 유행성 설사 바이러스(KPEDV-9)주를 이용하여 세포내 증식성을 비롯한 혈청학적 역학조사를 수행하였던 바 다음의 결과를 얻었다. 돼지 유행성 설사바이러스 국내 분리주는 Vero 세포에 연속계대시 증식성이 증가되었으며 90대 계대시 $10^{5.5}TCID_{50}/ml$의 역가를 나타내었다. 조직배양 순화주를 이용하여 간염세포내에서 20Kb 이상의 RNA가 존재함을 확인할수 있었으며 전자현미경 검사시 5~10nm의 외피항원 및 80~300nm크기의 coronavirus특징을 나타내었다. 설사증상을 나타내는 돼지의 장가검재료를 이용하여 유행성 설사 바이러스의 감염실태를 조사하였던 바 18%에 상당하는 감염 양성율을 확인하였으며 ELISA법에 의한 항체검사결과 전국적으로 약 45%의 항체 양성율을 나타내었다. Korean isolate, porcine epidemic diarrhea virus (KPEDV-9) was adapted through serial passages in vero cell. The viral yield reached up to $10^{5-6}$ $TCID_{50}/ml$ at the passage level of 90th. The cell adapted virus was characterized through genetic and morphological examinations. The RNA extracted from virus infected cell revealed the presence of RNA band with molecular size of >20Kb. The electron microscopic examination on purified virus showed the pleomorphic appearance of enveloped particles with 5-10nm surface projections, which fit with the shape of coronavirus. The etiological survey on swine diarrhea by immunofluorescence test(FA) indicated 17.5% positive rate on the PEDV infection. In addition, the incidence were detected both in piglets within two weeks old as well as fattening pigs. Serological survey by ELISA revealed the overall 45% positive result, thus, indicating the PEDV infection are widespread throughout this country.
돼지 폐포탐식세포로부터 adenovirus의 국내분리주 작성
권창희,조재진,권병준,송재영,채찬희,Kweon, Chang-hee,Cho, Jae-jin,Kwon, Byung-joon,Song, Jae-young,Chae, Chanhee 대한수의학회 1997 大韓獸醫學會誌 Vol.37 No.1
One cytopathogenic virus was isolated in alveolar macrophages from pig without any apparent respiratory clinical signs. Biophysical properties and electron microscopy of the isolate showed the characteristics of adenovirus. Intracytoplasmic inclusion bodies were seen in virus-inoculated cells. The genetic analysis indicated the presence of DNA with the size of >20Kb. In a serological survey of 40 serum samples collected from two different farms in slaughter house, 9 sera were positive for neutralizing antibody against the isolate. The potential implications of the isolate as the causative agent in respiratory disorder were discussed.
Isolation of porcine reproductive and respiratory syndrome virus(PRRSV) in Korea
권창희,권병준,이한정,조재진,황의경,신진호,윤용덕,강영배,안수환,김용희,허원,전무형,Kweon, Chang-hae,Kwon, Byung-joon,Lee, Han-jung,Cho, Jae-jin,Kang, Yung-bai,An, Soo-hwan,Kim, Yong-hee,Huh, Won,Jun, Moo-hyung,Wensvoort, G.,Yoon, Yong-d The Korean Society of Veterinary Science 1994 大韓獸醫學會誌 Vol.34 No.1
유사산 태아의 폐, 청색증을 나타내는 자돈으로부터 돼지생식기 및 호흡기증후군(PRRS)의 원인체로 추정되는 바이러스주(KPRRSV) 들을 분리하였다. 분리된 바이러스주는 돼지콜레라, 돼지오제스키병, 돼지뇌심근염바이러스에 대한 형광항체반응에서는 음성이었으며 기니픽혈구에 대한 혈구응집 능력을 나타내지 않았다. 그리고 포유 마우스의 뇌내 접종시 이상을 나타내지 않았으나 돼지생식기 및 호흡기증후군에 대한 형광항체검사시 양성반응을 나타내었다. 분리된 바이러스는 돼지폐포탐식세포(porcine alveola macrophages)에서 세포변성효과(cytopathic effect)를 나타내었으며 세포변성효과를 나타내었던 바이러스주중 일주(KPRRSV-1)를 돼지폐포탐식세포에서 7대 연속 계대하여 돼지에 접종한 후 혈청을 분리하여 미국 및 유럽지역에서 분리된 돼지유행성 유사산 및 호흡기증후군의 바이러스를 탐식세포에 감염시켜 효소면역방법 (immunoperoxidase monolayer assay)으로 분석한 결과 분리된 바이러스는 미국형 돼지호흡기 및 유사산증후군에 가까운 항원형으로서 판명되었다. Three viral strains, causing CPE in porcine alveolar macrophage cell, were isolated from aborted fetus, serum from young pig showing blue-ear sign and lung of suspected pig, respectively. The differential diagnostic results showed no characteristics of Aujeszky's disease virus(ADV), hog cholera virus (HCV), Japanese encephalitis virus(JEV), porcine parvovirus(PPV) and encephalomyocarditis virus (EMCV). However, positive reactions were demonstrated by IFA using monospecific porcine antibodies against Lelystad virus. When the paired sera of experimentally inoculated swine with one of isolate, KPRRSV-l were tested by IPMA, the result indicated that the isolate was related to United States isolate than European LV.
Pool fire에서의 화염의 높이 계산에 관한 실험식의 비교연구
황운기,권창희,Hwang, Woon-Gi,Kwon, Chang-Hee 대한안전경영과학회 2017 대한안전경영과학회지 Vol.19 No.1
In this study, the height of the flame required to estimate the heat flow path and flame spread in pool fire has been applied by the empirical formula, but it is calculated without applying the pressure and temperature parameters of the fire room. Until now, the height of the flame applied to pool fire was $l_F=0.235Q^{2/5}-1.02D$ in the Heskestad empirical formula, but accurate temperature calculation was not possible due to the temperature and pressure which are not influenced by the flame height. Therefore, applying the temperature and pressure around it can calculate the exact flame height, which can be applied to fire investigation and fire dynamics. The structure of the flame is divided into a continuous flame, an intermittent flame, and a buoyancy flame, but it is assumed that the flame height is calculated from the visual aspect to the intermittent flame region, and the temperature of the buoyancy flame is very low. The effect of heat of vaporization on the height of flame was investigated. The results showed that flame height was different according to the pressure and temperature around the fire room.
난황항체를 이용한 돼지 대장균 설사증 방제기법 개발 I. 대장균 pilus 항원과 LT로 면역시킨 닭의 면역반응
우승룡,김종만,권창희,이희수,임숙경,김종염,Woo, Seng-ryong,Kim, Jong-man,Kwon, Chang-hee,Lee, Hee-su,Lym, Suk-kyoung,Kim, Jong-yeom 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.4
Immunogenicity of Escherichia coli pilus and LT were evaluated in 20-week-old hens. The antigens were consisted of K88, K99, 987p pilus and heat labile toxin purified from enterotoxigenic Escherichia coli. The durations of antibody titers in sera and egg yolk were investigated by an enzyme-linked immunosorbent assay(ELISA). After first inoculation, antibody titers in sera reached at peak 2 weeks postinoculation. However, peak antibody titers in egg yolk were detected 4 weeks postinoculation, indicating that transfer of immunoglobulin from serum to egg yolk took about two weeks period. Although there were slight reduction in titers, the specific antibodies in egg yolk lasted up to 3 months. Immune responses against monovalent and combined antigens were showed as almost same patterns. The transfer rate of antibodies from serum to egg yolk didn't show any significant differences among three pilus antigens in this study. Considering the concentrations of antigens in each inoculated group, multivalent antigens containing heat labile toxin of E coli were found to be more immunogenic than monovalent antigen in producing specific antibodies. From this experiment, it was demonstrated that multivalent antigens containing three pilus and heat labile toxin could be a promising candidate for the production of egg yolk antibodies for prophylactic use in preventing swine colibacillosis in future.
나방현(Nah, Bang-Hyun),권창희(Kwon, Chang-Hee),박래훈(Park, Rae-Hoon),윤형국(Yoon, Hyung-Goog) 한국산학기술학회 2010 한국산학기술학회논문지 Vol.11 No.7
유비쿼터스 환경에서 획득되는 정보들은 시공간의 틀 속에서 서로 만나 관계성을 가지게 됨으로써 지식화되 고, 업무 또는 어플리케이션 모델과 결합하여 지능화된 콘텐츠로 변화하게 된다. 따라서, 유시티 콘텐츠를 구성하는 개체들은 복잡한 관계들을 가진다. 유시티 콘텐츠는 사용자의 의도를 파악하여 개인화된 응답을 제공하는 지능성을 갖추어야 한다. 본 연구에서는 SPIRIT의 공간 온톨로지 설계 방법을 확장하여 사람 또는 사물에 대하여도 유형, 관 계, 인스턴스의 세 가지 온톨로지로 구성하였다. 공유된 개념의 관계 모델이 없는 경우의 인스턴스들 간의 관계는 어 떤 시공간적 사건을 통한 관계로 설정하였다. 이렇게 함으로써 문자열 매칭에 의한 연관어에 비하여, 반드시 의미적 관련성을 갖는 연관어들을 추천할 수 있게 하였다. Information in UbiComp Environment are transformed to knowledge by relationship in a spatio-temporal location, and then became intelligent contents with task procedures or application models. The entities in U-City has lots of relationships. It is important in U-City contents to provide intelligent and personalized response to meet the intention of users. We extend the spatial ontology model of SPIRIT to other domain. Domain ontologies are consist of type, relation, and instance ontologies. When the relationship model by shared concepts are not defined, we used the spatio-temporal events to find relationships. So we proposed the methods to recommend semantically related terms, not syntactically.