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- 저자 : 권영명 ( Sun Hi Lee (검색결과 5 건)
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인삼엽(人蔘葉)의 엽록체발달(葉綠體發達)과 $CO_2$ 고정양상(固定樣相)에 관한 연구(硏究)
이순희,조영동,홍영남,권영명,Lee, Sun-Hi,Cho, Young-Dong,Hong, Young-Nam,Kwon, Young-Myung 생화학분자생물학회 1982 한국생화학회지 Vol.15 No.2
인삼엽의 greening과정중 엽록체발달(葉綠體發達), $CO_2$ 고정능(固定能), 초기(初期) 광합성(光合成) 중간대사산물(中間代謝産物), catalase와 glycolate oxidase 활성등을 조사(調査)하였고, 이러한 결과(結果)들은 CP-complex의 생성(生成)과 연관시켜 논의(論議)하였다. 엽록체발달양상(葉綠體發達樣相)은 타식물(他植物)과 같았으나 그 과정(過程)이 비교적 느렸고, 따라서 $CO_2$고정능(固定能)도 느렸다. 12시간 이상(以上) 빛을 조사(照射)하여야 엽록소양(量)이 증가하였다. 이러한 결과(結果)는 엽록체발달(葉綠體發達)에 따라 thylakoid막(膜)에서 CP-cmplex의 생성(生成)의 증가와 일치하였다. $CO_2$고정능(固定能)은 다른 양지식물(陽地植物)에 비하여 훨씬 낮았으며, 초기(初期) 광합성(光合成) 중간대사산물(中間代謝産物)의 변이(變異)와 glycolate oxidase 및 catalase 등의 활성(活性)으로 보아 인삼은 $C_3$-plant임을 추정할 수 있었다. Development of chloroplast, ability of $CO_2$-fixation, photosynthetic early intermediates and activities of catalase and glycolate oxidase were observed during greening process. Such results were discussed with the formation of CP-complex. The phenomenon of chloroplast development was similar to other plants, but the rate of process was relatively slow, and ability of $CO_2$-fixation was slow accordingly. The increase in chlorophyll was observed after more than 12 hours illumination. Such results were paralleled with the formation of CP-complex in thylakoid membrane. Considering the low ability of $CO_2$-fixation compared with that of other sun-plants, different photosynthetic intermediates at early period, and activities of both glycolate oxidase and catalase, ginseng is more likely to be $C_3$-plant.
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이순희,조영동,홍영남,권영명 ( Sun Hi Lee,Young Dong Cho,Young Nam Hong,Young Myung Kwon ) 생화학분자생물학회 1982 BMB Reports Vol.15 No.2
Development of chloroplast, ability of CO₂-fixation, photosynthetic early intermediates and activities of catalase and glycolate oxidase were observed during greening process. Such results were discussed with the formation of CP-complex. The phenomenon of chloroplast development was similar to other plants, but the rate of process was relatively slow, and ability of CO₂-fixation was slow accordingly. The increase in chlorophyll was observed after more than 12 hours illumination. Such results were paralleled with the formation of CP-complex in thylakoid membrane. Considering the low ability of CO₂-fixation compared with that of other sun-plants, different photosynthetic intermediates at early period, and activities of both glycolate oxidase and catalase, ginseng is more likely to be C₃-plant.
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권영명(Young Myung Kwon),이진범(Jin Bum Lee),이순희(Sun Hi Lee),조영동(Young Dong Cho) 한국식물학회 1979 Journal of Plant Biology Vol.22 No.3
Catalase, glycolate oxidase, hydroxypyruvate and NADPH-glyoxylate reductases activities in cell free extracts from leaves of 3 cultivars, Suwon 264, IR 36 and Jin Heung of rice plants were studied at different growing stages. Catalase and glycolate oxidase shows inclining activities toward the maximum vegetative growth whereas declining activities in either the enzymes were noticed during the maturing stage. After the photopeiodic condition exhibit increasing hydroxypyruvate and NADPH-glyoxylate reductases activities with time until maturing stage. No significant differences were found in the enzyme activities, and in analytical data of nitrogen, chlorophyⅡ contents, dry weight and soluble proteins among the 3 cultivars.
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Greening 에 따른 보리 어린식물의 전자전달과 광인산화반응 활성의 변화
이진범,홍영남,이순희,조영동,권영명 ( Chin Bum Lee,Young Nam Hong,Sun Hi Lee,Young Dong Cho,Young Myung Kwon ) 생화학분자생물학회 1983 BMB Reports Vol.16 No.1
The development of activities of electron transport and photophosphorylation at various stages of greening was studied with the isolated chloroplast in dark-grown, 7-day-old barley seedlings. The activity of total photosystem(P5 II+I) reached the peak at 4 hrs of illumination, after which it sharply decreased (FeCy, DCPIP, or MV as acceptor) . After 8hrs greening it increased gradually. The activity of photosystem I (PS I) showed the similar pattern with that of PS II+I during the initial greening time, but it was decreased gradually after 8 hrs greening. That of photosystem II (PS II) , however, was increased continuously up to 48hrs. The content of chlorophyll a and b increased abrubtly with greening and the ratio of chl a/b after 8 hrs of illumination reached about 3 : 1. In the absence of photophosphorylation, the pH changes of chloroplast suspension were induced by illumination. In these cases, the acidity per ㎎ chlorophyll was decreased sharply whereas its per mg protein did not show great difference. The photophosphorylation was not detected in the isolated chloroplast within 16 hrs greening, and the pH change produced by ATP formation was observed after 24 hrs greening.
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Greening에 따른 보리 어린식물의 전자전달과 광인산화반응 활성의 변화
이진범,홍영남,이순희,조영동,권영명,Lee, Chin-Bum,Hong, Young-Nam,Lee, Sun-Hi,Cho, Young-Dong,Kwon, Young-Myung 생화학분자생물학회 1983 한국생화학회지 Vol.16 No.1
어두운 곳에서 7일간 기른 보리(Hordeum vulgare L. cv. Baecdong) 어린식물을 750 ft.c의 빛에서 greening시킬 때 일어나는 광화학반응 및 광인산반응의 변화를 분리 엽록체를 재료로 조사하였다. PS II+I의 활성은 greening 초기(4hr)에서 최대값을 보인 뒤 급속히 감소하다 8시간 이후부터 점진적으로 증가한 반면, PS I의 활성은 초기에서 보인 최대값은 PS II+I 활성과 같은 양상을 보였으나, 8시간 이후 오히려 감소하는 경향을 보였다. 그러나 PS II 활성은 greening 시킴에 따라 계속적으로 48시간까지 증가하였다. 엽록소 a와 b의 함량은 greening 되면서 급격히 증가하였으며, 특히 chl a/b 값은 8시간 이후에는 거의 3 : 1의 비율이 유지되었다. 광인산화반응이 얼어나지 않는 조건밑에서 엽록체 현탁액의 pH 변화는 빛에 의해 유발되었으며, 엽록소량으로 표시할 경우 acidity는 급격한 감소 현상을 보였고, 단백질량으로 표시한 경우에는 커다란 변화를 나타내지 않았다. 광얀산화반응은 16시간이내의 greening 처리를 한 보리의 분리 염록체에서는 검출되지 않았고, greening 시간을 24시간 이상으로 연장할 때 비로서 뚜렷한 ATP 형성에 의한 pH 변화가 관찰되었다. The development of activities of electron transport and photophosphorylation at various stages of greening was studied with the isolated chloroplast in dark-grown, 7-day-old barley seedlings. The activity of total photosystem(PS II+I) reached the peak at 4 hrs of illumination, after which it sharply decreased (FeCy, DCPIP, or MV as acceptor). After 8hrs greening it increased gradually. The activity of photosystem I (PS I) showed the similar pattern with that of PS II+I during the initial greening time, but it was decreased gradually after 8 hrs greening. That of photosystem II (PS II), however, was increased continuously up to 48hrs. The content of chlorophyll a and b increased abrubtly with greening and the ratio of chl a/b after 8 hrs of illumination reached about 3 : 1. In the absence of photophosphorylation, the pH changes of chloroplast suspension were induced by illumination. In these cases, the acidity per mg chlorophyll was decreased sharply whereas its per mg protein did not show great difference. The photophosphorylation was not detected in the isolated chloroplast within 16 hrs greening, and the pH change produced by ATP formation was observed after 24 hrs greening.
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