Morphometrical Changes on Korean Rose Bitterling, Rhodeus uyekii, in Early Growth Period

구인본,박인석 한국발생생물학회 2014 발생과 생식 Vol.18 No.1

We investigated the process of yolk absorption in Korean rose bitterling, Rhodeus uyekii, and determined the changes in its morphometric characteristics. The R. uyekii from 1 days post hatching (DPH) to 21 DPH, the eye diameter (ED) was 5.4 at 5 DPH. Thereafter, the ED/total length (TL) ratio increased to 10.7 at 21 DPH (p<0.05). The yolk length (YL) decreased from 95.4 to 1.1 by 21 DPH, and this rate of decrease was greater than that for any other dimension (p<0.05). 12 morphometric dimensions/TL for the R. uyekii were measured at each sampling day from 21 DPH to 170 DPH. At just hatching, the average TL and BW were 6.1±0.09 mm and 4.9±0.07 mg, respectively. At 53 DPH, the average TL was 12.9±0.28 mm and the average BW was 14.7±0.72 mg; the total length growth equation was TL=5.507e0.038t (R²=0.916). Further, the body weight growth equation was BW=3.3647e0.0296t (R²=0.9354). The dimensions with regard to body depth showed the greatest growth rates in the external characteristics of the fish (p<0.05). The patterns of the morphometric characteristics measured in this study can be classified in three ways. The patterns were shown to be increased (y=0.0992x+ 12.07, R²=0.8333), decreased (y=–0.0569x+42.029, R²=0.8395) or maintained (y=0.005x+18.263, R²=0.3678) from 21 DPH to 170. These results will provide useful indices for the successful rearing of the R. uyekii.

메기(Silurus asotus) 기아 시 조직학적 변화

구인본,김영수,박인석,Goo, In Bon,Kim, Young Soo,Park, In-Seok 한국환경생물학회 2020 환경생물 : 환경생물학회지 Vol.38 No.2

메기(Silurus asotus)의 기아 시 생존율, 생식소 중량지수(gonadosomatic index; GSI), 간 중량지수(hepatosomatic index; HSI) 및 장 중량지수(intestinosomatic index; ISI)와 신장세포, 장 상피세포 및 간세포의 조직학적 변화를 조사하였다. 생존율은 섭이군이 92.2±0.47%, 기아군이 74.4±2.59%를 보였으며, GSI, HSI 및 ISI는 기아군에서 가장 낮은 값을 나타내었다(p<0.05). 신장세포 핵의 높이, 장 상피세포 핵의 높이, 간세포 핵의 크기 및 간세포의 크기는 섭이군에서 가장 높은 값을 보였으며(p<0.05), 신장세포핵의 높이, 장 상피세포 핵의 높이 및 간세포 핵의 크기는 기아군에서 가장 낮은 값을 보였다(p>0.05). 멜라닌대식세포(melano-macrophages; MMs)는 기아 동안 신장에서 증가함을 관찰하였다. 신장의 MMs 증가 및 간세포의 수축현상은 메기의 기아 상태를 식별하는 지표로 이용할 수 있으며, 본 연구의 결과는 메기의 영양 상태 확인을 위한 기초 자료로서 의미가 있을 것으로 사료된다. A 210-day experiment was conducted to examine the effects of starvation on survival, the gonadosomatic index (GSI), hepatosomatic index (HSI), and the intestinosomatic index (ISI), and histological changes in the renal tubule epithelium, midgut epithelium, and hepatocytes in Far Eastern catfish (Silurus asotus). The survival rate decreased to 92.2±0.47% in the fed group and 74.4±2.59% in the starved group during the 210-day experimental period. GSI, HSI, and ISI were lowest in the starved group (p<0.05). The hepatocyte nuclear area, hepatocyte cell area, the nuclear height of the midgut epithelium, and the nuclear height of the kidney were highest in the fed group (p<0.05). The hepatocyte nuclear area, nuclear height of the midgut epithelium, and nuclear height of the kidney were lowest in the starved group(p<0.05). The numbers of melano-macrophages (MMs) found in the kidney cells increased during starvation in this species. This suggests that thinner body cavity regions, the contraction of hepatocyte nuclear sites, and the spreading of kidney cell MMs in this species could be used as alternative indicators for identifying starvation conditions. Therefore, the results from our study provide accurate indications of the nutritional status of Far Eastern catfish.

Stimulation of Spermiation by Human Chorionic Gonadotropin and Carp Pituitary Extract in Grass Puffer, Takifugu niphobles

구인본,박인석,길현우,임재현 한국발생생물학회 2015 발생과 생식 Vol.19 No.4

Spermiation was stimulated in the mature grass puffer, Takifugu niphobles, with an injection of human chorionic gonadotropin (HCG) or carp pituitary extract (CPE). Spermatocrit and sperm density were reduced, but milt production was increased in both the HCG and CPE treatment groups relative to those in the control group (P < 0.05). These results should be useful for increasing the fertilization efficiency in grass puffer breeding programs.

Early Gonadogenesis and Sex Differentiation in the Korean Rose Bitterling, Rhodeus uyekii

구인본,김정은,김명훈,최혜성,공희정,이정호,박인석 한국발생생물학회 2016 발생과 생식 Vol.20 No.4

This report describes the sex differentiation of the Korean rose bitterling, Rhodeus uyekii, from hatching to 170 days post-hatch (DPH) in relation to total length (TL), body weight (BW), and integral water temperature (IWT). The growth curve of TL from just hatching to 83 DPH was 5.144e0.045t (R² = 0.961; t, time), and that of BW was 2.398e0.086t (R² = 0.725). Primordial germ cells (PGCs) were observed at 17 DPH (7.9 mm TL, 3.74 mg BW, 374°C IWT), and thereafter began to protrude into the peritoneal cavity. At 21 DPH (9.2±0.14 mm TL, 4.8±0.07 mg BW, 462°C IWT), some PGCs contained condensed chromatin and oocyte were observed in meiotic prophase. In contrast to the ovaries, which grew gradually after sexual differentiation, testes began multiplying at 25 DPH (10.1 mm TL, 5.42 mg BW, 550°C IWT), when testicular differentiation was first identified, and multiplied continuously thereafter. At 33 DPH (11.2 mm TL, 10.5 mg BW, 726°C IWT), the developing testes contained spermatogonia that exhibited mitotic activity. No spermatocyte or sperm cell was observed until 83 DPH (18.9 TL, 48.2 mg BW, 1,826°C IWT). At 170 DPH (32.5 mm TL, 270.1 mg BW, 3,740°C IWT), which was the end point of this study, the mature ovaries showed germinal vesicle breakdown, while the mature testes contained observable spermatocytes and sperm cells. These results allow us to identify the sex differentiation type of the Korean rose bitterling as differentiated gonochoristic.

Comparison of Cell and Nuclear Size Difference between Diploid and Induced Triploid in Marine Medaka, Oryzias dancena

구인본,임재현,길현우,임상구,박인석 한국발생생물학회 2015 발생과 생식 Vol.19 No.3

The influence of triploidization on cell and nucleus size characteristics of the same tissues of erythrocyte, retina, kidney, hepatocyte and midgut epithelium in marine medaka, Oryzias dancena has been determined histologically. Induced triploid fish are produced by cold shock treatments. Likewise, the size of horizontal cell nucleus in inner nuclear layer of retina, ganglion cell nucleus in ganglion cell layer of retina, proximal tubule cell of kidney, hepatocytes and nuclear height of midgut epithelium all appear to be significantly larger than diploid (P<0.05). On the other hand, retina thickness is larger in diploid than induced triploid (P<0.05). Induced triploid shows low density of cell number. Results of this study suggest that same characteristics in the induced triploid exhibiting larger cells and nucleus sizes with fewer number of cells than the diploid can be useful criteria for the distinction between diploid and induced triploid, and also the ploidy level in marine medaka.

Human chorionic gonadotropin(HCG) 및 carp pituitary extract(CPE)에 의한 복섬, Takifugu niphobles 의 배정 효과

구인본,길현우,박인석 한국발생생물학회 2013 한국발생생물학회 학술발표대회 Vol.2013 No.8

병행 Java 프로그램의 공유변수 접근사건 선택을 위한 투명한 감시도구

구인본 ( In-bon Kuh ),김영주 ( Young-joo Kim ),강문혜 ( Moon-hye Kang ),전용기 ( Yong-kee Jun ) 한국정보처리학회 2007 한국정보처리학회 학술대회논문집 Vol.14 No.2

병행 Java 프로그램의 경합은 프로그램의 비결정성을 초래하므로 반드시 탐지되어야 한다. 이러한 경합을 수행 중에 탐지하기 위해서는 모든 접근사건들을 감시할 수 있어야 한다. 기존의 경합탐지 기법들은 대상 프로그램을 수정하여 감시하므로 모든 감시지점을 인식하는 것은 현실적으로 어렵다. 본 연구에서는 JDI (Java Debug Interface)를 이용하여 모든 접근사건을 감시하여 선택할 수 있는 투명한 감시도구를 제안한다. 그리고 벤치마크 프로그램을 이용한 실험결과를 분석하여 투명성을 보인다.

Anesthetic Effects and Physiological Responses of Lidocaine-HCl in Siberian Sturgeon, Acipenser baerii

In-Bon GOO(구인본),In-Seok PARK(박인석),Chul-Hong PARK(박철홍),Yoon-Kwon NAM(남윤권) 한국수산해양교육학회 2019 水産海洋敎育硏究 Vol.31 No.2

병행 Java 프로그램의 확장적 경합탐지를 위한 JDI 기반의 투명한 감시도구

김영주,구인본,배병진,전용기,Kim, Young-Joo,Kuh, In-Bon,Bae, Byoung-Jin,Jun, Yong-Kee 한국정보처리학회 2009 정보처리학회논문지 A Vol.16 No.2

병행 Java 프로그램의 경합은 프로그램의 비결정성을 초래하므로 반드시 탐지되어야 한다. 이러한 경합을 수행 중에 탐지하기 위해서는 스레드에 대한 수행양상과 모든 접근사건들을 감시할 수 있어야 한다. 기존의 경합탐지 기법들은 프로그램의 수행중에 기록된 파일들을 분석하거나 대상 프로그램을 수정하여 감시하므로 스레드나 모든 접근사건들에 대한 감시가 현실적으로 어렵다. 본 연구에서는 JDI(Java Debug Interface)를 이용하여 스레드에 대한 수행양상과 모든 접근사건을 감시하여 확장적 경합탐지를 할 수 있는 투명한 감시도구를 제안한다. 여기서 JDI는 JDPA(Java Platform Debugger Architecture)에서 제공하는 상위 레벨의 100% 순수 자바 인터페이스로써 자바프로그램의 수행중에 특정 정보를 제공할 수 있다. 그리고 제안된 도구의 투명성을 입증하기 위해서 벤치마크 프로그램으로 실험한 결과, 모든 스레드와 접근사건들을 프로그램 수정없이 감시할 수 있었고 프로그램의 감시시간이 20배 이상 증가되었다. Race conditions in current Java programs must be detected because it may cause unexpected result by non-deterministic executions. For detecting such races during program execution, execution flows of all threads and all access events can be monitored. It is difficult for previous race detection techniques to monitor all threads and access events in actuality because these techniques analyze the files traced during program execution or modify original source programs and then monitor these programs. This paper presents a transparent scalable monitoring tool to detect races using JDI(Java Debug Interface) where JDI is 100% pure java interface to provide in JDPA(Java Platform Debugger Architecture) and is able to provide information corresponding to events occurred in run-time of programs. This tool thus can monitor execution flows of all threads and all access events without program modification. We prove transparency of the presented tool and grasp the efficiency of it using a set of published benchmark programs. As a result of this, the suggested tool can monitor all threads and accesses of these programs without their modification, and their monitoring time is increased to more than 20 times.

메기(Silurus asotus) 기아 시 외부형태 및 체 절단면 변화

박인석,구인본,길현우,Park, In-Seok,Goo, In Bon,Gil, Hyun Woo 한국환경생물학회 2021 환경생물 : 환경생물학회지 Vol.39 No.1

메기(Silurus asotus)를 대상으로 210일간 기아 시 계측형질 및 체 절단면 형질을 측정하여 그 기아 효과를 조사하였다. 실험 종료시 섭이군의 생존율은 92.2±0.47%였으며, 기아군에서는 74.4±2.59%의 생존율을 보였다. Ls, DALPO/Ls, DADOP/Ls, ED/Ls, CH/Ls, BDAA/Ls, BDMA/Ls 및 BWAA/Ls의 8개 항목에서 유의한 차이를 보였으며(p<0.05), 다른 16개 항목 DALAD/Ls, DPDPL/Ls, HPLAA/Ls, HALAV/Ls, HALOP/Ls, DAUPO/Ls, DADAV/Ls, DADAA/Ls, Max BL/Ls, Man BL/Ls, LD/Ls, HWOP/Ls, DAUF/HL, DAUS/HL, IW/HL 및 DAUE/HL에서는 초기군, 섭이군 및 기아군의 세 그룹 간 유의한 차이가 없었다(p>0.05). 섭이군은 각 체 절단면에서 기아군보다 더 높은 전체 높이(THX), 더 넓은 너비(WX) 및 더 넓은 면적(AX)을 보였다. 본 연구는 메기의 기아 상태에 대한 외형 및 체 절단면 형질에 대한 정보를 제공한다. 이는 메기에 대한 먹이 조절에 대한 방법을 보조하고 영양상태를 나타내는 기본 기초자료로 활용될 수 있을 것이다. 또한, 본 연구에서 사용한 조사 분석 방법을 타 어종에서 적용 시 그 영양 상태에 대한 정보를 파악하는 데 유용하게 활용될 수 있을 것으로 사료된다. A 210-day experiment was conducted to examine the effects of starvation on survival, external morphometric traits, and sectioned morphometric traits in Far Eastern catfish(Silurus asotus). The survival rate decreased to 92.2±0.47% in the fed group and to 74.4±2.59% in the starved group. Eight dimensions of Ls, DALPO/Ls, DADOP/Ls, ED/Ls, CH/Ls, BDAA/Ls, BDMA/Ls, and BWAA/Ls showed significant differences (p<0.05), and 16 other dimensions of DALAD/Ls, DPDPL/Ls, HPLAA/Ls, HALAV/Ls, HALOP/Ls, DAUPO/Ls, DADAV/Ls, DADAA/Ls, Max BL/Ls, Man BL/Ls, LD/Ls, HWOP/Ls, DAUF/HL, DAUS/HL, IW/HL, and DAUE/HL were not significantly different among the initial, fed, and starved groups (p>0.05). The fed group showed higher total height (THX), wider width (WX), and larger area (AX) than those of the starved group in each body section. This research provides information on the morphometric traits and body sections of starved Far Eastern catfish. These results can be used as a basic guide to indicate nutritional status and assist in aquaculture methods for Far Eastern catfish. Moreover, the methods used in this study were considered helpful because the survey analysis method can be used to investigate information on the nutritional status of other fish species.