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해수와 피조개에서 Vibrio mimicus K-1 의 생존에 대한 보관온도의 영향
고병호(Byeong Ho Koh),이원동(Won Dong Lee),안성기(Sung Kee Ann),김지희(Ji Hoe Kim),이명숙(Myung Suk Lee) 한국수산과학회 1997 한국수산과학회지 Vol.30 No.2
The cell density changes of Vibrio mimicus K-1 in sea water and arkshell feeding it were examined at various temperature. The strain was suspended in sterilized sea water and storaged at experimental temperature (5, 10, 15, 20, and 28℃). At intervals of up to 10 days, aliquots of each suspension were plated onto BHI agar. At 5 and 10℃, the plate counts of V. mimicus K-1 showed a rapid decline, which is known to be a result of this bacterium`s entering into the viable but non culturable state. At 20 and 28℃, however, V. mimicus K-1 are stable over the 10 days experimental periods. V. mimicus K-1 was fed to arkshell, which was subsequently stored at temperatures ranging from 5 to 20℃ for 10 days. The samples of arkshell were homogenized and plated at intervals to determine the cell density of V. mimicus K-1 and total aerobic population of bacteria present. At 5 and 10℃, the numbers of V. mimicus K-1 in sea water rapid decreased over the 10 days experimental periods. However, little change of V. mimicus K-1 densiiy was observed in shellstock arkshell at 5 and 10℃. While, V. mimicus K-1 density was decreased more rapidly to level below limit of dectection in shucked arkshell at same temperature. Incubation at the higher temperature (20℃) resulted in large increase in total aerobic bacterial number of shellstock arkshell. These results suggest that even with proper storage, indigenous levels of V. mimicus may remain sufficiently high in shellstock arkshell to produce infection in compromise hosts.
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Candida boidinii에 의한 Alcohol - Oxidase의 생성
이명숙(Myung-Suk Lee),김미은(Mee-Eun Kim),고병호(Byeong-Ho Koh),김상현(Sang-Hyun Kim) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.22 No.6
Candida boidinii CBS 8106과 Candida boidinii CBS 2428의 배양조건과 탄소원에 따른 alcohol-oxidase의 생성능과 생성된 효소의 기질 특이성을 실험하였다. 배양조건에 따른 효소 생성은 glucose를 첨가한 mineral salt medium에서 균체를 정상기까지 배양한 뒤 methanol이 첨가된 배지에서 재배양하는 2단계 배양법이 가장 효율적이었고 이 때 효소 최대 생성치는 Candida boidinii CBS 8106과 Candida boidinii CBS 2428 각각 1.46μmoles/min/㎎과 1.32μmoles/min/㎎을 나타내었다. 효소 생성시 탄소원으로서는 1.0%의 methanol이 가장 좋았고 기타 탄소 화합물의 경우, 단독 사용한 경우에는 효소생성을 볼 수 없었으나 methanol과 혼합 사용한 경우는 lactose, galactose, glycerol 그리고 saccharose의 경우는 methanol 단독 사용시 보다 오히려 효과가 좋았다. C. boidinii가 생성한 alcohol-oxidase는 기질로써 methanol을 가장 잘 이용하였고 탄소쇄가 길어질수록 이용율을 감소하여 탄소수 5개까지의 직쇄 alcohols과 일부 불포화 alcohol에만 활성을 나타내었다. The synthesis of alcohol-oxidase[EC 1.1.3.13] was investigated in the yeasts, Candida boidinii CBS 8106 and C. boidinii CBS 2428, during growth on different carbon sources. Alcohol-oxidase was undetectable in all strains submitted to the test in the mineral salts medium containing 1.0% glucose, but its production was rapidly increased when the carbon source was changed glucose to 1.0% methanol after 48hrs of incubation. When cells were grown on the various carbon sources (glucose, xylose, lactose, glycerol, galactose, saccharose, sorbose, lactic acid or acetic acid), the alcohol-oxidase activity was undetected. These carbon sources together with methanol yielded far better synthesis of alcohol-oxidase than in the case of carbon sources alone. Alcohol-oxidase was active towards alcohol of shorter alkyl-chain length than C_5 and unsaturated alcohols. Its affinity for these alcohols decreased with the increasing length of the alkyl-chain. The apparent Km values for the methanol of Candida boidinii CBS 8106 and C. boidinii CBS 2428 were 1.96 and 1.21, respestively.
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이원동(Won Dong Lee),장동석(Dong Suck Chang),고병호(Byeong Ho Koh),이명숙(Myung Suk Lee),정은탁(Eun Tak Jeong) 한국수산과학회 1997 한국수산과학회지 Vol.30 No.2
The study was attempted to detemine the ratio of stomach and intestine to viscera gathered on catching vessels, the critical level of VBN content as a freshness quality of viscera of Alaska pollack for raw materials of Changran-jeotgal. It also examined the changes of VBN values, pH and viable cell counts during the fermentation. Then sensory evaluation of the fermented products, Changran-jeotgal, was done according to the freshness of raw materials used. The ratio of stomach and intestine to the gathered viscera on the vessel was about 72%, while that of round state of Alaska pollack was about 18%. There was no significant relationship in VBN content between fish muscle and viscera. It suggests that VBN content is not a reasonable freshness criteria in viscera but in fish muscle. However, if we use the VBN content as a freshness criteria of viscera for raw materials of Changran-jeotgal, less than 70 ㎎% of VBN value could be recommended. According to the experimental results, the product yields and sensory evaluation scores were no good not only for economical evaluation but also for consumption.
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연안 해수에서 분리된 Vibrio mimicus K-1 의 특성
이원재,이명숙,고병호 한국수산학회 1994 한국수산과학회지 Vol.27 No.3
식중독 원인균으로 알려진 Vibrio mimicus(V.mimicus)의 분포 상태와 이의 생리적 특성을 조사하기 위하여 1993년 1월부터 9월까지 민락동과 광안리 해수욕장 부근의 해수를 채취하여 V. mimicus의 월별 분포 상태를 실험하였으며, 여기서 분리된 균주 중에서 항생제 내성이 강한 균으로 분리, 동정된 V. mimicus K-1의 생리적 성질과 배양 특성을 조사하였다. 수온이 16.3℃인 4월부터 검출되기 시작하여 8월까지 검출되었으나, 수온이 15℃ 이하인 겨울철(1, 2, 3월)에는 전혀 검출이 되지 않았다. 분포 정도는 최소 0.4(MPN/100 ㎖)에서 최고 70((MPN/100 ㎖)으로 지역에 따라 차이가 있었다. V. mimicus K-1은 37℃, pH 7.5, 그리고 염도 0.5%에서 각각 최대의 증식율을 보였다. 그리고 항생제(colistin, erythromycin, bacitracin, tetracycline, penicillin)에 대해서 내성을 나타내었다. 자외선(30W, 50㎝)에 대해서는 상당히 내성이 약했으며, 70초간 조사 후에는 검출이 되지 않았다. 열에 의한 치사율은 50℃에서 5.7분, 60℃에서 2.1분, 그리고 70℃에서는 0.7분 만에 최초균수의 90%이상이 사멸하였다. An environmental study was done to examine the distribution of Vibrio mimicus in aquatic environments of Kwangan and Minrak beach, Pusan, Korea. Moreover, both bacteriological characteristics and lethal effects of isolated V. mimicus were observed. Sea water samples were collected monthly from January to September, 1993, and quantitatively analyzed for V. mimicus. This organism was isolated from April(water temperature was 16.3℃), whereas it was not isolated when the water temperature fell below 15℃. V. mimicus counts were not remarkably high, however this study at least describes the distribution and occurrence of the possible highest density in aquatic environments of this region. Among the confirmed V. mimicus strains, the author chose the strongest antibiotic resistant bacterium and named it V. mimicus K-1. This strain has antibiotic resistance to colistin, erythromycin, tetracycline, and penicillin, and most isolates had a higher level of antibiotic resistance than V. mimicus ATCC 33653. The optimum growth for V. mimicus K-1 was observed at 37℃, pH 7.5, and 1% NaCl, respectively. This organism was mostly inactivated by Ultra Violet irradiation (30W, 50℃) for 70 seconds and death lethality increased in proportion to treatment temperature (D_(50)=5.7 min, D_(60)=2.1 min, and D_(70)=0.7 min).
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