자연과학편 : 다양한 트레이닝 강도가 글루코스 운반 단백질과 미토콘드리아 효소 증가에 미치는 영향

강호율(HoYoulKang),(JohnL.Ivy) 한국체육학회 1992 한국체육학회지 Vol.31 No.2

The effects of high(HI) and low(LI) intensity exercise training were examined on the change of insulin-regulatable glucose transporter protein(Glut-4) content, and the activity of oxidative enzyme citrate synthase in the epitrochlearis of obese Zucker(fa/fa) rats. Sedentary(SED) and lean(LN) Zucker(Fa/?) rats were used as controls. The content of Glut-4 protein was determined by Westem-blotting and the citrate synthase activity was measured by the method of Srere. No significant difference in Glut-4 protein content was observed between LN and SED rats. However, there was a significant difference between SED and HI rats in Glut-4 protein content(p<0.05). The difference between HI and LI rats approached significantance(p<0.09). Citrate synthase activity was not significantly different between LN and SED rats. but there was a significant difference between SED and exercise trained groups(p<0.05). Between LI and HI rats, citrate synthase activity was significantly different. Glut-4 protein content in epitrochlearis of obese rats was significantly correlated with cirtrate synthase activity(r=0.55). The results suggest that the increased in Glut-4 protein content due to exercise training is related to the degree of training.

운동생리학 : 걷기 운동시 탄수화물과 탄수화물 / 단백질의 섭취가 에너지 산화와 저혈당증 발전에 미치는 영향

강호율(HoYoulKang) 한국체육학회 2001 한국체육학회지 Vol.40 No.3

본 연구는 걷기 운동 (50% VO₂max)시 운동 전 탄수화물 또는 탄수화물/단백질의 혼합 섭취가 운동초기 저혈당 (hypoglycemia) 발전파 에너지 대사에 미치는 영향을 조사하는 것이 목적이다. 신체 건강한 남자 대학생 8 명을 대상으로 운동시작 30분전에 500 ㎖의 음료를 섭취시키고 30분이 경과한 후 트레드밀에서 3분간의 준비운동을 실시한 다음 각 피험자의 50 % VO₂max 의 운동강도에서 60 분간 걷기를 실시하였다. 실험에 사용된 음료는 비교 (CON ; water), 탄수화물 (CHO ; glucose 58g), 탄수화물/단백질 (CHO/Pro ; glucose 58 g + protein 15.8g)이었고, 3종류의 음료를 무작위로 배당하여 피험자들에게 섭취시켰으며, 각 처치간 간격은 7 일 이상으로 하였다. 채혈 시기는 음료섭취 전, 음료섭취 후 10, 20, 30분, 운동을 시작한 후부터는 매 10분마다 채혈하였다. 운동 전 혈당 반응에서 각 처치간 유의한 차이가 있었으며 (CHO > CHO/Pro > CON), 운동 개시 20분 후에 CHO/Pro에서 혈당수준이 64.8±4.7 ㎎/㎗로 저혈당증에 근접하였다. 그러나 CHO는 운동 개시 후 유의한 감소가 있었지만 저혈당 수준으로까지 발전되지 않았다. 운동전 인슐린 반응에서 CHO/Pro는 CHO보다 유의하게 높았다. 그리고 운동 개시 20분까지 인슐린은 CHO/Pro와 CHO가 CON보다 유의하게 높은 수준으로 유지되었다. 이러한 인슐린증가는 CHO/Pro와 CHO에서 운동중 CON에 비하여 유의하게 높은 탄수화물의 산화율을 유지하였으며, FFA와 glycerol은 유의하게 낮게 유지되었다. CON은 운동 중 유의한 성장 호르몬의 증가를 나타내었고, CHO/Pro는 저혈당증이 발생한 시점이후부터 혈중 성장호르몬 농도가 증가하였다. 따라서 운동 전 탄수화물과 단백질의 복합섭취는 탄수화물 단독섭취보다 인슐린 분비를 촉진시켜 운동초기 저혈당증의 발전에 유의한 영향을 미치는 것으로 나타났다. The purpose of this study was to examine the effects of pre-exercise carbohydrate or carbohydrate-protein consumption on transient hypoglycemia development during walking exercise. Six male college students were volunteered for this study. Each subject consumed 500 ㎖ of test beverage 30 min prior to exercise and then performed 60-min walking exercise at 50% of VO₂max. Each subject completed three randomly assigned treatments which were a water (CON), carbohydrate (carbohydrate 58g; CHO), and carbohydrate/protein (carbohydrate Sag and protein 15.8 g; CHO/Pro) beverage. Five milliliter of venous blood was drawn every 10 min for 90 min of whole experimental period. Plasma glucose (GLU), lactate (LA), insulin (INS) and free-fatty acid (FFA) concentrations were determined. LA concentrations during exercise were not significantly different among all treatments. GLU responses prior to exercise were significantly different among all treatments (CHO > CHO/Pro > CON). In CHO/Pro, GLU level (64.8±4.7 ㎎/㎗) at 20 min of initial exercise was significantly dropped to almost hypoglycemia level in the comparison with pre Glu level (80.8±2.4 ㎎/㎗). However, GLU (75.1±5.4 ㎎/㎗) in CHO was significantly decreased as compared with pre GLU level (84.2 ± 1.9 ㎎/㎗), but this decrease could not be considered to be a hypoglycemia condition. INS responses prior to exercise were significantly different among all treatment (CHO/Pro > CHO ) CON). At the 20 min of the initial exercise, INS levels in CHO (12.4±1.1 μU/㎖) and CHO/Pro (10.6±1.0 μU/㎖) were significantly greater than that in CON (7.7±0.3μU/㎖). Therefore, our results suggest that a mixture diet of carbohydrate and protein is more effective than carbohydrate diet alone to develop the transient hypoglycemia as a pre-exercise meal.

자연과학편 : 지구성 운동이 SD-rat의 성장호르몬 유발 인슐린 저항성에 미치는 영향

강호율(HoYoulKang),정수련(SuRyunJung),정현령(HyunLyungJung),나현종(HyunJongNa),공보경(BoKyungKong) 한국체육학회 2006 한국체육학회지 Vol.45 No.4

본 연구의 목적은 지구성 운동이 SD-Rats의 성장호르몬 유발 인슐린 저항성에 미치는 영향을 조사하는 것이다. 28 마리는 4 집단 (각 7마리)으로 무선 배정하였다: CON(멸균증류수 투여집단), GH(성장호르몬 투여집단, 0.4 IU/kg/day, 6 days/week), Ex(지구성운동집단, 24 m/min, 2h, 5 days/week), GH/Ex(성장호르몬 투여/지구성 운동집단). 8 주간의 처치후 혈중 지질, 간과 근육 (plantarise)내 중성지방량, 골격근의 포도당 유입률, 경구당부하검사를 측정하였다. 연구결과 경구당부하검사시 GH의 혈당과 인슐린 반응이 나머지 3 집단에 비해 유의하게 높은 것으로 나타났다. 골격근의 포도당 유입률은 최대하 인슐린 농도에서 GH가 CON에 비해 유의하게 낮은 것으로 나타났으나, GH/Ex와 CON은 유의한 차이가 나타나지 않았다. 혈중 FFA 농도는 GH, GH/Ex가 CON에 비해 유의하게 높게 나타났고, 근육의 중성지방량은 GH가 CON에 비해 유의하게 높은 것으로 나타났다. 구연산합성효소의 활성도는 EX, GH/Ex가 CON, GH에 비해 유의하게 높게 나타났다. 이러한 결과를 통해 8 주간의 성장호르몬 투여와 지구성운동의 병행은 성장호르몬 유발 인슐린 저항성을 상쇄시키는 것으로 나타났다. The purpose of this study was to investigate the effects of endurance exercise on hGH-induced insulin resistance of skeletal muscle in rats. 28 rats were randomly divided into 4 groups: CON (saline injection group), GH (hGH injection group, 0.4 IU/kg/day, 6 days/week), Ex (Exercise group), GH/Ex (hGH injection/ Exercise group). After 8 weeks of treatment, the plasma lipids profiles, triglyceride contents in muscle & liver, muscle glucose transport rates (GTr), OGTT, and citrate synthase activities (CS) were measured. Plasma Insulin and glucose levels in GH were significantly increased than other 3 groups during OGTT. The GTr was significantly lower in GH than CON. However, there was not a significant difference between GH/Ex and CON. FFA levels were significantly higher in GH, GH/Ex than CON. TG contents in plantaris muscle was significantly greater in GH than CON, Ex. CS was significantly higher in EX, GH/Ex than CON, GH. However GH injections in GH and GH/Ex did not affect the plasma lipids except plasma FFA compared to CON and Ex. In conclusion, regular exercise training attenuated GH-induced insulin resistance in rats.

운동생리학 : 운동전 탄수화물 또는 탄수화물/단백질 혼합물의 섭취가 에너지 대사에 미치는 영향; Glycemic index

정수련(SuRyunJung),강호율(HoYoulKang) 한국체육학회 2003 한국체육학회지 Vol.42 No.3

The purpose of this study was to examine the effect of glucose and cornstarch pre-exercise meals with and without protein on glycemic, insulinemic, and substrate oxidation responses during both a postprandial period and prolonged exercise. The subjects in this study were 9 healthy untrained college male students. All subjects participated in five exercise trails. One of five experimental treatments, randomly assigned, was ingested 60 min before cycling for 90 min at 60% of VO2max. The experimental treatments consisted of glucose (Glu; glucose 2 g/kg BW), glucose and protein mixture (Glu/Pro; glucose 1.6 g/kg BW and protein 0.4 g/kg BW), starch (Sta; starch 2 g/kg BW), and starch and protein mixture (Sta/Pro; 1.6 g/kg BW and protein 0.4 g/kg BW). The starch used in the current study was raw cornstarch powder. The water treatment was served as control (CON; H_2O). Blood samples were taken just before ingestion and every 15 min of exercise. Plasma samples were analyzed by duplicated for glucose, lactate, free fatty acid and insulin. The expired gas was analyzed for the determination of CHO and fat oxidation rate during the exercise. when Sta was supplemented, plasma insulin and glucose responses were significantly lower than Glu, and plasma FFA level, fat oxidation rate were significantly higher than Glu. However, Sta/Pro was significantly reduced fat oxidation compared to the Sta. Therefore, protein supplementation in carbohydrate pre-exercise meal has a negative effect on endurance performance.

스포츠 심리학 : 트레드밀 운동과 Clenbuterol 투여가 쥐의 체지방 축적과 근효소의 활성에 미치는 영향

이민형(MinHyungLee),이수천(SooChunLee),강호율(HoYoulKang) 한국체육학회 1998 한국체육학회지 Vol.37 No.4

본 연구는 트레드밀 운동과 Clenbuterol(β₂-adrenergic agonist)의 장기간 투여, 그리고 두 가지를 복합하여 처치하였을 경우 체지방 함량과 근효소의 활성에 미치는 영향을 조사하는 것이 목적이다. 실험의 대상은 40마리의 SD(Sprague-Dawley)계 암컷 쥐(rat)로 하였으며, 무작위로 선정하여 각각 비처리군(CON), Clenbuterol 처리군(CL), 운동군(TR), 운동/Clenbuterol 처리군(TR+CL)으로 분리하였다. 훈련은 8%의 경사도로 동력으로 작동되는 treadmill을 이용하여 수행되었으며, 훈련기간은 주 5회 8주간 이루어졌다. Clenbuterol 투여방법은 물에 용해시킨 Clenbuterol을 1일 체중(kg)당 0.8mg씩 경구 투여하였다. 8주간의 운동트레이닝과 Clenbuterol투여는 집단간 성장률에는 유의한 변화가 없었다. 그리고 Clenbuterol투여와 트레이닝은 근육의 양을 유의하게 증가시켰으며, 체지방량은 유의하게 감소시켰다. 두 가지 처치의 복합적 영향은 근육 양의 증가에 있어서는 부가적이었으나, 체지방 양의 감소에 있어서는 부가적이지 않았다. 장기간의 Clenbuterol의 투여는 골격근에서 hexokinase와 citrate synthase활성의 유의한 감소를 가져왔다. 이와 같은 효소활성의 감소는 유의한 지구성 운동 수행력의 감소를 초래하였다. 그러므로, 본 연구의 결론은 체구성에 있어 Clenbuterol처치가 미치는 영향은 운동처치가 미치는 영향과 같으나, Clenbuterol투여와 운동처치는 각각 정상 쥐의 골격근 효소의 활성에 역으로 영향을 미치는 것으로 나타났다. The effects of exercise training, chronic administration of clenbuterol (β₂-adrenergic agonist), and the combination of these two treatments on body fat content and muscle enzyme activities were investigated. Forty male Sprague-Dawley rats were randomly assigned to one of four treatment groups: sedentary control (CON, clenbuterol (CL), exercise trained (TR), or exercise trained/clenbuterol (TR+CL). The training protocol consisted of running on a motor-driven treadmill up an 8% grade, 5 days/wk for 8 weeks. Rats receiving clenbuterol were intubated with 0.8 mg/kg body wt/day dissolved in deionized water. Exercise training and clenbuterol administration significantly not only reduced the body fat content as evidenced by the decreasing retroperitoneal fat weight, but also increased the skeletal muscle mass without the changes of growth rate. The combined effect of these two treatments in increasing the muscle mass was additive, but in reducing the fat content, it was not additive. Chronic clenbuterol treatment significantly reduced the hexokinase and citrate synthase activities in skeletal muscle. These decreased enzyme activities significantly reduced endurance performance. Thus, the results of the current study indicate that the effects of clenbuterol treatment are the same as the effects of exercise training in body composition, but each of the clenbuterol treatment and exercise training inversely affects the changes in skeletal muscle enzyme activities of normal rats.

운동생리학 : 다양한 당원지수의 탄수화물 섭취가 지구성 운동능력에 미치는 영향

정수련(SuRyunJung),박신우(SinWooPark),김승환(SeungHwanKim),강호율(HoYoulKang) 한국체육학회 2004 한국체육학회지 Vol.43 No.4

The purpose of this present study was to determine the effect of various glycemic diets on endurance exercise performance. The eight healthy college students volunteered for this study (Height: 174.9±2.1 cm, Body weight: 70.0±2.2 kg, O2max: 46.2±2.7 ml/kg/min). After a 12-hour fasting, subjects were exercised at 65% of maximum oxygen uptake (O2max) for depletion of the muscle glycogen and then they drunk beverage and took a rest for 60 minutes. After that, subjects cycled at 75% of maximum oxygen uptake to exhaustion. Meals consisted of 1.5g/kg body weight of either Glucose (Glc, GI=99) or Sucrose (Suc, GI=60) or Starch (Sta, GI=15) or Water (CON). The amount of the each beverage was 500 ml and its temperature keep in cool condition (≒ 2~5 ℃). The four kinds of beverage were ingested in random order and the spans between each beverage were over seven days. Ingestion of Glc and Suc resulted in significantly high plasma glucose and insulin concentrations in comparison with the ingestion of the Sta and CON during 1 hour after ingesting beverage. Glc, Suc and Sta showed more prolonged time to exhaustion compared with CON. Ingestion of Suc resulted in significantly high plasma lactate concentrations in comparison with the ingestion of the Glc, Sta and CON during 1 hour after ingesting beverage. No differences were observed in cortisol, heart rate and RPE. These results suggest that there is no effect of glycemic index on endurance exercise performance.

운동생리학 : 크레아틴 경구 투여가 골격근 특성 및 근력 발현에 미치는 영향

장지훈(JeeHunJang),복영(YoungBok),박정배(JeongBaePark),강호율(HoYoulKang),안나영(NaYoungAhn),김창근(ChangKeunKim) 한국체육학회 2001 한국체육학회지 Vol.40 No.2

이 연구는 탄성 저항 트레이닝 기간 중 크레아틴(Cr)의 경구 여가 등속성 근력과 근지구력, 무산소성 파워 및 능력에 미치는 영향을 구명하고자 하는 목적으로 수행되었다.동일한 운동 부하로 4주간 탄성 저항 트레이닝을 실시하던 중 3주초부터 5일간 매일 Cr을 20g씩 경구 투여한 집단 (Cr 투여집단, n=5)과 비투여 집단 (대조집단, n=5)으로 구분하였으며, 투여 전 ·후 전뇨를 채집하고 근생검을 실시하여 근섬유 형태를 분석하였다. 또한 하지의 굴 ·신근력은 등속성 근력 측정 장비를 이용하여 측정하였으며, 무산소성 파워 및 능력은 자전거 에르고미터를 이용하여 트레이닝 실시 전 ·후 5주간 매주 측정하였다.실험 결과 대조군에서는 type I 섬유의 비율이 증가하고 type IIx 섬유의 비율이 감소하였으나(P$lt;.05), Cr 투여 그룹에서는 typeIIa와 IIx 섬유의 비율이 증가하였다. 또한 근섬유 횡단면적과 근섬유당 모세혈관 수에 있어서도 Cr투여 그룹에서 증가되었다. 트레이닝 후 하지신근력에 있어서는 Cr 투여 그룹에서 감소하고 대조군에서는 증가하였으나, 하지굴근력에 있어서는 Cr 투여 그룹에서 증가하고 대조군에서는 감소하였다. 무산소성 파워 및 능력에 있어서는 두 그룹 모두 증가하였다.한편 Cr 투여 후의 뇨량의 변화로 근육 내로의 Cr loading을 추정할 수 있어, Cr 투여 그룹을 다시 세분하여 분석한 결과, 근섬유 조성비 및 하지신근력의 결과는 서로 다르게 나타났다. The purpose of this study was to investigate the effect of creatine (Cr) supplementation on muscle power and muscle morphology.10 healthy male subjects were randomly assigned to 2 groups; one was control (n=5), and the other Cr supplementation (n=5) group. Elastic resistance strength training was equally performed for 4 weeks in both groups. Cr was loaded (20g×5days) in the 3rd week after beginning the training. Before and after training, whole urine was collected, and measured quantitatively. Muscle sample was taken by muscle biopsy technique, and prepared for histochemical analysis. Isokinetic muscle power and endurance were measured by Cybex- II 6000, and anaerobic power and capacity by MONARK cycle ergometerIn the Cr supplementation group, relative distribution of type I muscle fiber was increased and that of type II x was decreased (p$lt;.05). Mean cross-sectional area and the number of capillaries per fiber were increased, consequently diffusional area was increased. With the leg flexion power was increased in the Cr supplementation group whereas decreased in the control group. However, the contrary results were shown in the leg extension power Anaerobic power and capacity were increased in both groups.When the Cr supplementation group was subdivided by the reduction in urine volume just after Cr loading, relative distribution of type IIx fiber and the leg extension power were increased in T-Cr where as decreased in F-Cr. This result suggested the possibility of underestimating or misreading the effect of Cr oral supplementation.

자연과학편 : Isocaloric high-fat diet와 지구성 운동이 인슐린 저항성과 미토콘드리아 생합성에 미치는 영향

정수련(SuRyunJung),권대용(DaeYongKwon),김진환(JinHwanKimg),강호율(HoYoulKang) 한국체육학회 2012 한국체육학회지 Vol.51 No.3

본 연구는 4주령의 male Wister rat을 대상으로 4주간 등열량 고지방식이 (50% calories from fat) 또는 지구성 운동 (treadmill running, slop 8%, 23m/min, 120분/일, 5일/주)이 미토콘드리아 생합성과 인슐린 저항성에 미치는 영향을 알아보는 것이다. 4주간의 등열량 고지방식이는 일반 사료 집단에 비해 체중과 체지방량에 차이가 없었고, 혈중 포도당, 인슐린 수준 및 골격근내의 인슐린 유발 포도당 이동률도 4주간 등열량 고지방식이 및 일반 사료 집단 간에 차이가 없는 것으로 나타났다. 4주간 등열량 고지방식이와 지구성 운동을 병해 실시한 집단의 체중, 체지방, 혈중 포도당 수준도 다른 3집단과 차이가 없는 것으로 나타났다. 그러나 지구성 운동을 실시한 집단은 고지방식이 집단보다 혈중 인슐린 수준이 유의하게(p<0.05) 낮게 나타났다. 4주간의 등열량 고지방식 또는 지구성 운동은 미토콘드리아 생합성을 증가시키지 않았으나 복합처치의 경우 유의하게(p<0.05) 증가시켰고, 이러한 현상은 혈중 유리지방산의 증가와 함께 나타났다(p<0.05). 이상의 연구 결과들을 종합하면 4주간의 등열량 고지방식과 지구성 운동 복합처치는 인슐린 저항성을 발생시키지 않고 미토콘드리아 표지인자를 유의하게 증가시킴으로 미토콘드리아 생합성을 안정적으로 증가시키는데 좋은 대안이 될 것으로 생각된다. The purpose of this research was to find effects of high fat diet and endurance exercise on mitochondria biosynthesis and insulin resistance in male Wister rat. Th rats were randomly separated into 4 groups: A, Isocaloric high fat diet group (50% of calories from fat), B. Chow group, C. Isocaloric high fat diet with endurance exercise (treadmill running, slop 8%, 23 m/min, 120 min/day, 5 days per week), D. Chow group with endurance exercise. Both Isocaloric high fat diet group and chow group were given an equal caloric composition with 3.2kcal/g. equal amount of food were checked every day and given to both groups. 4 weeks of high fat diet did not show any change in body weight and amount of body fat. Further the level of glucose and insulin in blood, and insulin-stimuilated glucose transport rate in epitrochlearis muscle was not affected by Isocaloric high fat diet. However, the endurance exercise showed statistically significant change in the level of insulin in blood. Although either Isocaloric high fat diet or endurance exercise alone did not change on mitochondria biogenesis marker, Isocaloric high fat diet with endurance exercise could induce the increased level of marker (p<0.05). Also, plasma free fatty acids were increased in this group. From this investigation, Isocaloric high fat diet with moderate-intensity endurance exercise is effective way to induce mitochondrial biogenesis.

자연과학편 : 일회성 저항운동이 인슐린 감수성 및 혈중 젖산, CK에 미치는 영향

곽혜은(HyeEunKwak),정수련(SuRyunJung),정현령(HyunLyungJung),강호율(HoYoulKang) 한국체육학회 2007 한국체육학회지 Vol.46 No.5

본 연구의 목적은 일회성 저항성운동량의 변화가 회복기 OGTT동안의 혈당 변화에 따르는 인슐린 작용 및 인슐린 분비의 감소 기전에 대한 영향을 알아 보고자하는 것이다. 본 연구의 피험자는 K 대학교에 재학 중인 신체 건강한 남자 대학생 9명의 피험자를 연구대상으로 실시하였다. 일회성 저항성운동의 강도 10-RM(repetition maximum)을 통해, 저부하운동(Low-Volume Exercise: LVE) 처치는 총 3세트의 운동을 실시하였으며 고부하운동(High-Volume Exercise: HVE) 처치는 총 6세트의 운동을 실시하였다. 운동처치는 준비운동(5분)에 이은 본 운동(LVE; 약 60분, HVE; 약 120분)의 순서로 시행하였다. OGTT동안 혈당과 혈당AUC은 HVE가 LVE에 비해서 감소하는 경향을 나타냈고 LVE는 CON에 비해서 감소하는 경향을 나타났다. 또한 혈중 인슐린은 HVE가 CON에 비해서 유의하게 감소하는 경향을 나타냈지만(p<.05), LVE와 HVE의 인슐린AUC 감소는 CON에 비해서 유의한 차이는 없었다. 따라서 건강한 대학생의 저항성운동량이 클수록 혈당 반응은 비교적 큰 경향을 나타냈고, 그 운동량의 차이에 상관없이 인슐린 작용 및 분비의 감소를 통해서 전신적으로 인슐린감수성을 향상하였다. The purpose of this study was to investigate the effects of double single bouts of strength training on the areas under the response curve(AUCs) for glucose, insulin, and c-peptide during the oral glucose tolerance test(OGTT). The nine college male students were volunteered for this study. The exercise protocol consisted of either 3 sets of 8 exercises with 10 maximal repetitions(Low-Volume Exercise: LVE) or 6 sets of 8 exercises with 10 maximal repetitions(High-Volume Exercise: HVE). Plasma glucose responses were not significantly different among CON, LVE, and HVE during OGTT. The reduction in the glucoseAUC was greater in the LVE and HVE than in the CON, which were not significantly different. Plasma insulin responses at 60 min and 90 min during OGTT were significantly different between CON and HVE. Reduction of insulinAUC was greater in LVE and HVE than in CON. At 60min during OGTT, c-peptide response was also greater in LVE and HVE than in CON. Reduction of c-peptideAUC was greater(P<0.05) in the LVE and HVE than in CON. Therefore, the results of this study demonstrated that the glucose improvements were more or less dependent on the volume of resistance exercises in normal college male students. Additionally, the insulin and c-peptide responses would be affected from acute resistance exercises themselves, but not affected from the resistance exercise volume.

운동생리학 : 장기간 운동이 쥐의 골격근의 Proteasome 발현과 Protein Oxidation에 미치는 영향

신영오(YoungOhShin),박수연(SooYeonPark),김종걸(JongGulKim),오재근(JaeKeunOh),강호율(HoYoulKang) 한국체육학회 2004 한국체육학회지 Vol.43 No.4

Oxidatively modified proteins are continuously produced in cells by reactive oxygen and nitrogen species generated as a consequence of aerobic metabolism. 20S proteasome complex actively recognizes and degrades oxidized proteins. Our study examined the effect of exercise on proteasome expression and protein oxidation in rats. Twenty SD male rats were randomly divided into two groups(control group, CON; exercise group, EXE), and EXE received treadmill running exercise, fixed 8% grade, 25m/min, 2-hr/day, 5days/week for 8 weeks. Soleus and plantaris were removed after 48-hr undergone last performance. 20S proteasome alpha and beta subunits, and protein carbonyl derivatives were detected by immunoblotting and densitometry. Results are as follows: Expression of 20S proteasome alpha and beta subunits in EXE have a tendency to increment, specially alpha in soleus and beta in plantaris, but no statistical significance. Protein carbonyl levels increased in EXE about 43 kDa, 37 kDA, 29 kDa. Expression of 29 kDa-matched carbonyl derivative in soleus was significant and expression of 43 kDa-matched carbonyl derivative in plantaris was significant statistically. Totally, protein oxidation in soleus was larger than in plantaris. In conclusion, chronic exercise leads to production of more protein oxidation and also 20S proteasome subuints related proteolysis.