http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
헤스페리딘/PLGA 필름에서 망막색소상피세포의 부착과 증식거동
이소진(So Jin Lee),강수지(Su Ji Kang),김혜윤(Hye Yun Kim),이정환(Jung Hwan Lee),김은영(Eun Young Kim),권순용(Soon Yong Kwon),정진화(Jin Wha Chung),주천기(Choun Ki Joo),강길선(Gil Son Khang) 한국고분자학회 2014 폴리머 Vol.38 No.1
망막색소상피(retinal pigment epithelium, RPE)는 시기능을 유지하는데 중요한 역할을 하여 RPE의 퇴화는 여러 망막변성질병을 유발한다. 현재 이에 대한 효과적인 치료법이 부족하여 세포 이식에 적합한 지지체를 제작하기 위해, 생분해성 고분자인 PLGA와 항염증, 항산화 작용 등의 기능이 있는 헤스페리딘을 이용하여 하이브리드 필름을 제조하였다. ARPE-19를 파종한 후, MTT 분석법을 이용하여 세포 증식률을 확인하고, 세포의 부착 및 세포 형태를 SEM을 통하여 확인하였다. 또한 RPE 세포의 특이적 유전자 발현정도를 확인하기 위하여 RT-PCR을 수행하였고, RPE65의 발현을 확인하기 위해 AEC 면역화학적 염색을 실시하였다. 그 결과, 헤스페리딘/PLGA 필름은PLGA보다 RPE 세포의 부착, 증식 및 표현형 유지가 우수함을 확인하였고, 이를 통해 헤스페리딘/PLGA 필름의 망막재생을 위한 조직공학적 담체로써 응용 가능성을 확인할 수 있었다. Retinal pigment epithelium (RPE) plays an important role in maintaining the visual function and the degenerationof the RPE causes several retinal degeneration disease. In order to fabricate the suitable carrier for RPE transplantation,the hybrid poly(lactide-co-glycolide) (PLGA) film with hesperidin was prepared. Hesperidin has an antiinflammatoryand antioxidant characteristics. ARPE-19 was seeded on hesperidin/PLGA film and then, cell proliferationwas determined by the MTT assay, and cell adhesion and cell morphology were confirmed by SEM. Also, RT-PCR wasperformed to confirm the expression of the specific genes, and AEC immunohistochemical staining was performed todetermine the expression of RPE65. As a result, we confirmed that attachment, proliferation and phenotype maintenanceof RPE cells were more excellent on hesperidin/PLGA film than PLGA film, thereby we were able to confirm the potentialapplications of hesperidin/PLGA film as tissue engineering carrier for regeneration of retina.
F2211-04 생체조직공학적 의료제품(TEMPs)에 대한 표준 분류
소정원 ( Jung Won So ),김순희 ( Soon Hee Kim ),백미옥 ( Mi Ock Baek ),노혜원 ( Hye Won Roh ),이나리 ( Na Ri Lee ),유규하 ( Gyu Ha Ryu ),조양하 ( Yang Ha Cho ),이승진 ( Seung Jin Lee ),강길선 ( Gil Son Khang ) 한국조직공학과 재생의학회 2008 조직공학과 재생의학 Vol.5 No.1
Tissue engineered medical products (TEMPs) are those protocols and products developed for use in the human body as biological substitutes to restore, maintain, or improve tissue function. So TEMPs may achieve a therapeutic potential from cells, biomolecules, scaffolds, and other materials, and processed tissue and derivatives used in various combinations or alone. Since interactions may occur among the components used in TEMPs, new standard descriptions, test methods, and practices are needed to aid the evaluation of these interactions. Therefore this technical report the aspects of tissue engineered medical that will be developed as standards.
실크/PLGA 하이브리드 필름에서 실크가 인간 대동맥 내피세포의 부착과 증식에 미치는 효과
이지혜(Ji Hye Lee),이소진(So Jin Lee),김슬지(Seul Ji Kim),김경희(Kyoung Hee Kim),김영래(Young Lae Kim),송정은(Jeong Eun Song),이동원(Dong Won Lee),강길선(Gil Son Khang) 한국고분자학회 2013 폴리머 Vol.37 No.2
혈관내피세포는 혈관 안쪽을 덮고 있는 편평한 세포층으로, 혈관의 기능과 혈관평활근세포의 증식을 조절한다. 폴리락타이드글리콜라이드 공중합체(PLGA)는 물성이 좋고 분해속도를 조절하기 좋은 생분해성 합성고분자이며, 여러 형태로 제조하기 쉽다. 누에에서 얻은 실크 피브로인은 18가지 아미노산으로 구성되어 있고 세포의 부착과 세포 기능 유지에 중요하며 화장품, 의료분야 등 다양한 분야에서 응용되고 있다. 본 연구에서는 용매 증발법을 이용하여 0, 10, 20, 40 및 80 wt%의 실크를 이용하여 실크/PLGA 하이브리드 필름을 만들었으며, MTT, SEM, ELISA, 면역세포화학염색법을 실시하였다. 실크/PLGA 하이브리드 필름에서 실크 함량에 따른 인간 대동맥 내피세포의 부착과 증식을 측정한 결과, 40 wt%의 실크/PLGA 하이브리드 필름에서 세포의 부착과 증식이 가장 높았으며, 이런 결과들은 실크가 세포의 증식에 좋은 영향을 미치고 실크/PLGA 하이브리드 필름의 표면이 인간 대동맥 내피세포의 성장에 알맞은 환경이라는 것을 확인할 수 있었다. The vascular endothelial cells are the inner layers of blood vessels. It regulates the function of blood vessels and proliferation of vascular smooth muscle cells. Poly(lactide-co-glycolic acid) (PLGA) is a biodegradable synthetic polymer with a well-controlled degradation rate and an acceptable mechanical strength. It can be easily fabricated into many shapes. Silk consists of 18 amino acids. It found important for attaching cells cultured in vitro, and maintaining cell functions. In this study, we fabricated silk/PLGA biomaterial hybrid films of 0, 10, 20, 40 and 80 wt% silk. We performed MTT, SEM, ELISA, and immunocytochemistry analyses. We confirmed the adhesion and the proliferation of HAECs on silk/PLGA according to the content of silk, and 40 wt% silk/PLGA hybrid films have superior adhesion and proliferation properties. These results demonstrate that silk/PLGA hybrid films provide suitable surfaces for HAECs, and there is the effect of silk on cell growth and proliferation.
SIS/PLGA 필름에서 SIS 함량이 디스크세포의 증식에 미치는 영향
임지예 ( Ji Ye Lim ),김순희 ( Soon Hee Kim ),소정원 ( Jeong Won So ),최진희 ( Jin Hee Choi ),이종문 ( John M. Rhee ),신형식 ( Hyung Shik Shin ),강길선 ( Gil Son Khang ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.3
Porcine small intestine submucosa is accellular tissue and widely used as a biomaterials without immunorejection responses. Poly(lactide-co-glycilide) (PLGA) has controllable biodegradability and good biocompatibility. In this study, we evaluated 2-dimensional porcine small intestine submucosa (SIS)/PLGA hybrid films on adhesion, proliferation and phenotype maintenance of intervertebral disc cells. We prepared natural/synthetic hybrid films using 0, 10, 20, 40 and 80 wt% of SIS by the solvent evaporation method. SIS/PLGA films were characterized by scanning election microscopy (SEM). Disc cells were seeded on PLGA and SIS/PLGA film and we confirmed the effects of adhesion and proliferation on disc cells according to content of SIS by cell count and SEM observation. Contents of 10 wt% SIS positively affected to proliferate and maintain of disc phenotype in SIS/PLGA Film.
탈미네랄화된 골분/히알루론산을 이용한 조직공학적 지지체의 제조 및 특성분석
임지예 ( Ji Ye Lim ),김순희 ( Soon Hee Kim ),소정원 ( Jeong Won So ),이종문 ( John M. Rhee ),강길선 ( Gil Son Khang ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.3
Demineralized bone particle(DBP) that affects to cell proliferation and differentiation has been used to clinical trial as natural material. hyalunonic acid(HA) called natural moisturizer can be used as biomaterials. In this study, we tried to design and characterize novel DBP-HA sponge. DBP-HA sponge were prepared by freeze-drying method after addition of 1 wt% HA solution into fabricated DBP sponge. Sponge was crosslinked with 1-ethyl-(3- 3-dimethyl aminopropyl) carbodiimide hydrochloride(EDC) solution with 50 mM concentration for 24 hrs and lyophilized. We seeded cells in DBP-HA sponge. Cellular attachment was assayed by 3-(4,5-dimethylthiazole-2-yl)- 2,5-diphenyltertazolium-bromide(MTT) test. DBP-HA sponges were characterized by scanning electron microscopy, fourier transeform infrared spectrometer and water ubsorption ability. We demonstrated presence of HA in DBP-HA sponge from C-O functional group observed by the FT-IR analysis. Moreover, we confirmed high water uptake ability and high cell viability in DBP-HA sponge. Therefore, we could expect that DBP-HA scaffolds are applicable for the tissue regeneration.
PLGA 지지체에 함유된 DBP 함량이 줄기세포의 골분화에 끼치는 영향에 대한 in vitro 테스트
백미옥 ( Mi Ock Baek ),김순희 ( Soon Hee Kim ),소정원 ( Jung Won So ),임지예 ( Ji Ye Lim ),노혜원 ( Hye Won Roh ),이나리 ( Na Ri Lee ),유규하 ( Gyu Ha Ryu ),조양하 ( Yang Ha Cho ),이승진 ( Seung Jin Lee ),강길선 ( Gil Son Khang ) 한국조직공학과 재생의학회 2008 조직공학과 재생의학 Vol.5 No.1
Demineralized bone particles(DBP) exhibit osteoinductive properties and are very useful for bone formation and other orthopeadic applications. In this study, we investigated the osteogenic capabilities of rat bone marrow stromal cells(rBMSCs) in PLGA scaffold impregnated with various DBP contents. DBP-loaded PLGA scaffold (10, 20, 40, 80 % w/w) were prepared by solvent casting/salt leaching method. rBMSCs were seeded in PLGA and DBP/PLGA scaffolds, and then cellular viability and proliferation were assayed by water-soluble tetrazolium salt(WST-8) assay. Osteoblast differentiation of cells were analyzed by alkaline phosphatase(ALP) activity. Finally, reverse transcript polymerase chain reaction(RT-PCR) was assessed to measure mRNA expression of the genes (ALP, osteocalcine and type I collagen) of osteogenic marker in rBMSCs depending on various content of DBP. These results could demonstrated that presence of DBP in PLGA might play important roles in osteoblast differentiation of rBMSCs.
안현희 ( Hyun Hee Ahn ),김혜수 ( Hye Soo Kim ),이혜경 ( Hye Kyung Lee ),신소영 ( So Young Shin ),이형진 ( Hyung Jin Lee ),강길선 ( Gil Son Khang ),이해방 ( Hai Bang Lee ),이일우 ( Il Woo Lee ) 한국조직공학과 재생의학회 2005 조직공학과 재생의학 Vol.2 No.4
Adipose derived stromal cells (ADSCs) are very similar to MSCs and can differentiate into mesenchymal lineage and nonmesenchymal lineage. ADSCs are very easily obtained than MSCs and no pain to acquire cells. Recently researches revealed that MSCs have nonspecific immune suppressive effect on lymphocyte proliferation. We studied about whether nonself ADSCs have lymphocyte suppressive effect on mixed lymphocyte culture (MLR) like MSCs. First we obtained 1st passaged ADSCs and tested whether these cells have stem cell characteristics. We confirmed induced cell by ALP and Oil red O staining. For immune suppressive effect test, ADSC were co-cultured with reaction lymphocyte and stimulator lymphocyte. We defined nonself ADSCs could suppression lymphocyte proliferation in MLR and two more cell number treatment could suppression lymphocyte proliferation 5-fold. Accordingly we found out ADSCs can lymphocyte suppression by dose-dependent pattern. In nonspecific immune suppression test by PHA as non specific lymphocyte stimulator, ADSCs can suppression PHA induced lymphocyte proliferation. In conclusion, it is our belief that ADSCs cells can alternative sources of MSCs in clinically usage and also remain carefully treatment.