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Detection of Bifidobacteria by α - galactosidase activity
민해기,이시경,강국희 한국농화학회 1993 Applied Biological Chemistry (Appl Biol Chem) Vol.36 No.3
This method using the synthesis substrate of 5-bromo-4-chloro-3-indolyl-α-galactoside (X-α-Gal) was examined for the differential enumeration of Bifidobacteria and lactic acid-producing bacteria. Bifidobacteria possess a high level of α-galactosidase activity. Biffdobacterium longum KCTC 3215 exhibited the highest α-galactosidase specific activity (8.57 units/㎎ protein). Determination of α-galactosidase activity using the PNPG procedure showed that Lactobacillus, Streptococcus, Pediococcus, and Leuconostoc strain had lower α-galactosidase activity as compared to Bifidobacteria. The X-α-Gal based medium is useful to identify Bifidobacteria among lactic acid-producing bacteria since the enzyme action of α-glactosidase spilts X-α-Gal substrate and releases indol which impacts a blue color to Bifidobacterial colonies on agar plates. All strains of Bifidobacteria appeared as blue colonies on MRS agar medium supplemented with 100 μM X-α-Gal while colonies of other lactic acid-producing bacteria appeared white or light blue.
복압성 요실금의 정량적 평가를 위한 진단 알고리즘에 관한 연구
민해기 ( Hae Ki Min ),김주영 ( Ju Young Kim ),노시철 ( Si Cheol Noh ),최흥호 ( Heung Ho Choi ) 한국방사선학회 2018 한국방사선학회 논문지 Vol.12 No.2
골반저근은 골반기관을 지지하는 기능을 가지고 있으며 요자제를 유지하는 여성의 주요 하부조직이다. 골반저근의 약화는 복압성 요실금의 원인이 되는데, 이러한 골반저근의 기능 정도는 복압성 요실금의 병증 정도를 평가하는 지표로 사용될 수 있다. 이에 본 연구에서는 골반저근의 수축 압력을 측정하여 복압성 요실금의 병적 진행정도를 정량적으로 진단할 수 있는 요실금 진단 알고리즘을 제안하였다. 이를 위하여 골반저근의 수축압력 정보를 측정할 수 있는 시스템을 제작하였으며, 측정된 데이터의 특징 분석을 위한 측정 프로토콜을 제안하였다. 복압성 요실금 환자로부터 획득한 데이터를 이용하여 5개의 진단 파라미터를 추출하였으며, 이를 이용한 진단 알고리즘을 구현하였다. 임상시험을 통하여 진단 알고리즘의 정확성을 평가한 결과 80%의 정확성을 보였으며, 20%의 위양성 진단 결과를 보였다. 반면에 위음성 진단 결과는 확인되지 않았다. 본 연구에서 제안한 요실금 진단 알고리즘은 복압성 요실금의 병적 진행 정도를 정량적으로 진단할 수 있으며, 요실금 진단 시스템 개발에 활용될 수 있을 것으로 판단된다. Pelvic floor muscle is the main sub-system that maintains urinary continence. The weakness of pelvic floor muscles causes the stress urinary incontinence, and therefore the degree of functioning of pelvic floor muscles could be used as an index to assess the degree of stress urinary incontinence. In this study, the quantitative diagnosis algorithm was proposed to estimate the degree of stress urinary incontinence (SUI) by measuring the contraction pressure of pelvic floor muscle. For these reason, the contraction pressure measurement system from pelvic floor muscle was developed, and the measuring protocol was suggested to analysis the obtained data. As the results of clinical test, the proposed diagnosis algorithm shows the 80% of accuracy, and 20% of false positive diagnosis. On the other hand, false negative results were not confirmed. Consequentially, we thought that the proposed urinary incontinence diagnosis algorithm can quantitatively diagnose the progression of the stress urinary incontinence and it can be used for the development of the incontinence diagnosis system.
Streptococcus thermophilus SKD 1006의 β-Galactosidase 유전자 구조
민해기,강국희,성문희 成均館大學校 科學技術硏究所 1992 論文集 Vol.43 No.2
The results can be concluded that the pSK 001 contains a gene for β-gal from Str. thermophilus SKD 1006, and expressed in E. coli JM 109. A 4.2 Kb of Hpa II-Hpa II fragment(lac Z of SKD 1006) of pSK 001 was subcloned into the corresponding site of pUC 19, and named pSK 002. A 1.2 Kb of Bgl II -Bgl II fragment of pSK 002 was ligated into the corresponding site of pUC 19, and named pSK 003. The nucleotide sequences of two portions(222bp close to 5' , 143 by close to 3' end) of Bgl II -Bgl II fragment of pSK 003 were determined by Sanger, Chen and Seeburg methods. The nucleotide sequences are identical to those of the gene for β-gal of Str. thermophilus A 054.
Bifidobacterium longum KCTC 3215의 β-Galactosidase 유전자의 Cloning 및 대장균에의 발현
민해기,강국희,성문희 成均館大學校 科學技術硏究所 1992 論文集 Vol.43 No.2
The β-D-galactosidase(β-gal) gene from Bifidobacterium longum KCTC 3215 was cloned to isolate and characterize it for potential use as a selection marker in food-grade cloning vector. Chromosomal DNA from Bif. longum KCTC 3215 was cleaved with the restriction enzyme Sau3AI and ligated to pBR 322 for transformation into E. coli JM109. A β-galactosidase positive clone was detected by its blue color on a medium supplemented with 5-bromo-4-chloro-3-indolyl-β-D-galactoside. This transformant possessed a single plasmid, designated pSK 100, which contained, in addition to the vector DNA, a 5.2 Kilobase(Kb) Sau3A1 insertion fragment coding for β-gal activity. An extract from JM 109(pSK100) contained a β-gal protein with the same electrophoretic mobility as the β-gal from Bif. longum KCTC 3215. A restriction enzyme map of pSK 100 was consisted of PvuII, HincII, PstI,AccI, EcoRI, etc.