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동물로부터 분리한 Thermophilic Campylobacter의 Biotype 및 Serotype
김용환,마점술,강호조,차인호,Kim, Yong-hwan,Mah, Jum-sul,Kang, Ho-jo,Cha, In-ho 대한수의학회 1987 大韓獸醫學會誌 Vol.27 No.2
A total of 145 strains of thermophilic Campylobacter spp. isolated from the fecal specimens of 108 cattle, 120 pigs and 104 chickens. The isolation rates of Campylobacter jejuni from cattle, pigs and chickens were 36.1%, 38.3% and 28.8%, respectively. In the biotyping of 115 strains of C. jejuni, 49.6% were belonged to biotype I, 33.9% biotype II, 10.4% biotype IV and 6.1 % biotype III. Twenty-eight strains of C. coli were 78.6% of biotype I, 21.4% biotype II. Two strains of C. laridis belonged to biotype I and II. One hundred of 105 C. jejuni cultures were typable serologically and represented 13 serogroups Serotype 4, 5, 26, 27 and 36 were encountered most frequently. Eighteen of 23 C. coli cultures were typable serologically and represented 6 serogroups. Serotype 8, 20, 21 and 31 were encountered most frequently. In the comparison of frequency of serotype between animal species, serotypes 4, 30, 5, 26 and 27 were encountered relatively common in the cattle source isolates, serotypes 26 and 36 in the pigs, and 36 and 17 in the chickens. The serotypes of C. coli encountered most frequently were serotype 8 and 31.
김용환,마점술,Kim, Yong-hwan,Mah, Jum-sool 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.3
To investigate the epidemiological trait of intestinal diseases of animals caused by thermophilic Campyllobacter spp., isolation of etiological agent was carried out and the profiles of plasmids and the transfer of resistance plasmid in the isolated Campylobacter spp. were examined. The results were as follows. 1. A total of 110 isolates of C jejuni and C coli were subjected to the test for the presence of plasmid DNA. Of the isolates examined, 60% of the isolates were noted to harbor plasmid DNA. Plasmid occurrencer ate from pigs, chickens and cattle were 76.2%, 61.7% and 37.7%, respectively. The plasmids of a large molecular weight, ranging from 36 Md to 86Md, were identified with the strains of tetracycline resistant. 2. Transfer frequency of tetracycline resistant plasmids was higher in the case of the filter mating method than in the broth mating method by the factor of 10~1,000. 3. Tetracycline resistant plasmids of C jejuni were transferrable to C jejuni and C coli by conjugation. In a low frequency, the transfer of tetracycline plasmid was also possible to Vibrio parahemolyticus. However, it was impossible to transfer to Streptococcus fecalis, E coli and Vibrio cholerae. 4. Tetracycline resistant plasmids of C jejuni were impossible to transfer to Campylobacter spp. and related bacteria by transformation.
김용환,마점술,Kim, Yong-hwan,Mah, Jum-sool 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.3
To investigate the epidemiological trait of intestinal diseases of animals caused by thermophilic Campylobacter spp., isolation of etiological agent was carried out. Isolated Campylobacter spp. were biotyped, serotyped and the susceptibility of the isolates to antimicrobial agents were examined. Th results were as follows. 1. Isolation rates of Campylobacter spp. from 649 fecal materials of 208 cattle, 300 pigs and 141 chickens were 25.5%, 23.7% and 38.3%, respectively. 2. The majority of the 130 isolates of C jejuni was classified as biotype I(50.6%) and biotype II (34.6%). Most of the 46 isolates of C coli were biotype I (71.7%). 3. Isolated C jejuni strains showed 14 different serotype, and serotype 4, 26, 36 were most frequent. Isolated C coli strains showed 5 different serotype and serotype 31 and 21 were relatively common. 4. Isolated Campylobacter spp. were highly susceptible to nalidixic acid, amikacin, gentamicin, colistin and chlorampehnocol.
Polyurethance foam을 이용한 환자고정효과
김용환,정상화,김화영,임신택,오세동,Kim, Yong-Hwan,Jeong, Sang-Hwa,Kim, Hwa-Yeong,Im, Sin-Taek,O, Se-Dong 대한방사선치료학회 1992 大韓放射線治療技術學會誌 Vol.5 No.1
The immogilzer for control the patient movement during the radiation beam on was made of the polyurethane foam. The time consumption is abut 8 minutes for completely making the immobilizer. In this experimental study, the effects of using the individual immbilizer have shown that the patients have had always a same plsition with comfortable and high reproducigilities. Furthermore, it has shown the time for patient setup was decreased by using the individual patient immobilizer.
김용환,Kim, Yong-Hwan 한국작물보호협회 2012 자연과 농업 Vol.278 No.-
농업이 지속가능한 산업으로 발전하기 위해서는 정부, 소비자, 대학 그리고 농산업이 서로 협력하여 농업인이 생산성과 지속가능성이라는 두 마리 토끼를 잡을 수 있도록 법적 시스템 구축과 미래생명산업으로의 투자가 있어야 한다.
김용환,Kim, Yong-Hwan 한국작물보호협회 2010 자연과 농업 Vol.262 No.-
사람들은 유기농과 일반 농산물 사이에 선을 그어놓고 차별하지만 과학에는 이런 선이 존재하지 않습니다. 단지 각기 다른 주장을 하는 사람들이 자기에게 유리한 결과만을 인용하기 때문입니다. 소비자들에게 중요한 것은 이데올로기적인 옳고 그름의 논쟁이 아니라 무엇을 어떻게 먹일 것인가 하는 것에 대한 해답입니다.