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김용호,김석동,홍은희 韓國作物學會 1994 한국작물학회지 Vol.39 No.4
콩 저장 단백질의 대부분은 globulin이며, 이중 7S와 11S가 70% 이상을 차지한다. 따라서 콩 단백질의 조성개량을 위해서는 11S/7S 비율 조정이 우선되는데, 본 연구에서는 몇가지 콩 품종을 사용하여 7S와 11S를 분리 확인하고, 이들 분획 단백질을 이용한 두부제조시험을 실시하였다. 7S와11S는 콩 분말을 탈지한 후 pH를 조정하고 원심분리를 이용함으로써 분획할 수 있었고, 전기영동상(SDS-PAGE)에서 이를 확인할 수 있었다. 공시계통들의 11S/7S 비율은 1.29∼1.38을 나타내어 계통간 차이가 없었다. 한편 콩 종실 단백질을 7S와 11S로 분획한 후 7S와 11S를 비율별로 혼합하여 두부를 제조하고 물성을 측정한 결과 11S량이 많아질수록 두부의 경도, 탄력성, 응집성 등이 높게 나타난다. 따라서 11S와 7S의 비율을 조정함으로써 여러가지 용도에 알맞는 식품개발이 가능하리라 판단된다. Soybean seed consists of two major storage protein, the 7S and 11S globulins. For improving the quality of soybean seed protein, an increase of 11S/7S ratio would be a desirable objective because the 11S globulin contains much more the sulfur-containing amino acids than the 7S globulin. In this study, some soybean varieties were used to investigate the analyzing method for 7S and 11S globulins. 7S and 11S globulins couble be fractionated by their different solubilities in tris buffers. Adjusting the pH and tris concentration were major factors affecting the precipitation of the two globulins. And it was possible to screen the soybean genotypes having aberrant subunit compositions of the two globulins by an sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of total soybean proteins. The ratio of 11S to 7S globulin ranged from 1.29 to 1.38. This paper also dealed with the contribution of protein components in soybean seeds to the physical properties of soycurd. It indicated that the soycurd from crude 11S was remarkably harder than that from crude 7S, and springiness and cohesiveness were slightly higher in soycurd having higher proportion of 11S. So, it may concluded that proportion of protein components in soybean seed can be important factor which controls the suitability for soycurd or other foods.
대학이미지 포지셔닝전략에 관한 연구 -부산지역 사립대학의 이미지 평가값을 중심으로-
김용호 대한경영정보학회 2004 경영과 정보연구 Vol.15 No.-
Local universities are faced with rapid environmental changes in 2000's. Therefore local universities are required to research for more specialized marketing strategy such as positioning strategy. The objectives of this study are to examine what are the most important university images and image position of 7 universities'. In order to this study, 276 questionnaires collected from university in Busan area are analyzed by t-test, anova. The results of this study are summarized as follows (1) the most important university images are ranked in the order 1) prospect of getting employment(4.78), 2) possibility of development (4.35), 3) confidence(4.31), 4) research ability(3.97). It is true that practical images get high grade, traditional image such as research ability remains yet as a very import image. (2) Except (A, F) & (B, E) university, common position is not found among the 7 universities, therefore each university cultivate important image as G university dose. (3) Private university are underestimated by student of national university. There is a necessity for cultivating and promoting private university images. (4) Students of private university, particularly students of F university, overestimated their university images seriously.
장막 침윤이 없는 위암 환자에서 수술 후 보조적 화학 요법에 대한 전향적 연구 -중간 보고-
김용호,김병식,이창환,김완수,육정환,오성태,박건춘,Kim Yong-Ho,Kim Byung-Sik,Lee Chang-Hwan,Kim Wan Su,Yook Jung-Whan,Oh Sung-Tae,Park Kun-Choon 대한위암학회 2001 대한위암학회지 Vol.1 No.4
Purpose: We have carried out prospective randomized clinical trial to compare survival benefit and side effect among three postoperative adjuvant chemotherapeutic regimens in serosa-negative gastric cancer patients. Materials and Methods: Total 317 cases were recognized as serosa negative and randomized into three groups at operating room. Out of them, 172 cases were excluded because of various reasons and 135 cases were analyzed finally; Group A 36 cases, Group B 49 cases, Group C 50 cases. Group A were treated with intravenous FP combination therapy, group B with MF combination therapy and group C with oral $UFT^{(R)}$ (mixture of Tegafur and Uracil) for one year. The median follow-up period was 30 months. Results: $88.9\%$ of Group A, $83.7\%$ of Group B and $90.4\%$ of Group C received adequate chemotherapy. The complication rates of Group A ($44.4\%$) was significantly higher than group B ($20.4\%$) and group C ($24.0\%$)(P<0.05). Most frequent complications were nausea and vomiting. The 3-year survival rates and disease-free survival rates were $92.2\%$ and $89.9\%$ respectively (Group A: $96.6\%,\;87.8\%$, B: $90.3\%,\;87.7\%$, C: $95.7\%,\;93.8\%$). There were no significant differences in survival rate and disease-free survival rate among the three groups (P>0.05). Conclusion: This study might suggest that the survival benefit of postoperative adjuvant chemotherapy for gastric Pseudomonas aeruginosa, and therefore it may be a useful adjunct tool for detection of Pseudomonas aeruginosa infection in combination with other conventional techniques.
Vibrio vulnificus의 Cell Wall Protein A와 Lipopolysaccharide (LPS)의 추출 및 이들이 효소활성에 미치는 영향
김용호,이봉헌,박장수,강신원,Kim, Yong-Ho,Lee, Bong-Hun,Park, Jang-Su,Kang, Shin-Won 생화학분자생물학회 1994 한국생화학회지 Vol.27 No.5
Vibrio vulnificus에서 lipopolysaccharide(LPS)와 cell wall protein A를 추출하여 치사독성 및 Limulus겔화 활성을 측정한 후 LPS의 효소활성에 미치는 cell wall protein A의 영향을 조사하였다. 이 결과들을 Escherichia coli와 Salmonella typhimurium LPS 및 cell wall protein A의 것들과 비교하였다. V. vulnificus LPS의 치사독성($LD_{50}$는 138.6 mg/kg)은 E. coli LPS와 S. typhimurium LPS의 것(56.3 mg/kg와 37.5mg/kg)보다 낮았지만 세가지 LPS의 Limulus 활성은 모두 같았다(0.1 ng/ml). LPS와 cell wall protein A에 의한 alanine aminotransferase(ALT), aspartate aminotransferase(AST)와 lactate dehydrogenase(LDH)의 활성은 증가하였다. 한편 LPS의 효소활성에 미치는 cell wall protein A의 영향은 커서 LPS와 cell wall protein A를 혼합한 것의 효소활성이 LPS만의 것보다 컸다. Lipopolysaccharide (LPS) and cell wall protein A from Vibrio vulnificus were extracted, were tested the lethal toxicity and Limulus amebocyte gelation activity, and were examined the effect of cell wall protein A on the enzyme activity of LPS. These results were compared to those of LPSs and cell wall protein As from Escherichia coli and Salmonella typhimurium. The lethal toxicity of V. vulnificus LPS ($LD_{50}$ was 138.6 mg/kg) was lower than those of E. coli LPS (56.3 mg/kg) and S. typhimurium LPS (37.5 mg/kg), but the Limulus gelation activities of all LPSs were the same value (0.1 ng/ml). The results of enzyme activity by LPS and cell wall protein A have shown that the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) were increased. The cell wall protein A on the enzyme activities of LPS was effective; the enzyme activities of LPS-cell wall protein A mixture were greater than those of LPS only.