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RISS 인기검색어
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율무의 Callus 形成에 미치는 培地와 生長調節物質의 影響
皮玉姉,鄭丞根 충북대학교 농업과학기술연구소 1991 農業科學硏究 Vol.9 No.2
This study was conducted to provide the basic informations necessary for tissue culture of job's tears(Coix lacryma-jobi L.). Embryos excised from matured seeds of job's tears were cultured in different medium(MS, Miller, B5 and N6) and different concentrations and combinations of growth regulators (2, 4-D, IAA, NAA, BA and Kinetin) and leaf tissue of young job's tears plant was cultured in different sucrose concentrations and pH. The heighest ratio of callus formation was obtained in MS medium, which was followed in the order of N6, B5 and Miller medium. Fresh weight of callus was 454mg in MS medium supplemented with 2, 4-D 3mg/1. No other growth regulators alone but 2, 4-D induced callus. Callus formation was better in supplemented medium with 2, 4-D alone than with 2, 4-D+BA combination. The callus formation was best in the medium supplemented with 2-3 mg/1 of 2, 4-D. Callus was not induced in any combination of growth regulators without 2, 4-D. As BA and kinetin concentrations were increased, callus formation was reduced regardless of 2, 4-D conentration in 2, 4-D, BA and Kinetic combinations. 2, 4-D+BA combination was better than 2, 4-D+Kinetic combination for calls formation. Callus formation was induced best when medium was supplemented with sucrose 2% and its fresh weight was 161mg. Although callus was induced in pH range of 4,5-7.0, the best result was obtained in pH 6.0 and the fresh weight was 156mg.
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장해림,박병준,박승아,피옥자,박소영,백기엽 한국식물생명공학회 2016 JOURNAL OF PLANT BIOTECHNOLOGY Vol.43 No.1
Rosa rugosa is a medicinal, ornamental, and edible plant native to Eastern Asian countries, including Korea, Japan, and China. The aim of this study was to establish a system for biomass production and secondary metabolite accumulation during in vitro culture and acclimatization of Rosa rugosa. The highest rate of multiple shoot proliferation was achieved with 8.8 μM benzyladenine (BA) (83.3%). However, the number of shoots (14.4 per explant) at 4.4 μM BA was higher than that at 8.8 μM BA. Compared to BA, a combination of thidiazuron (TDZ) and indole butyric acid (IBA) exhibited significantly lower shoot induction, with only 50.0 ~ 79.2% and 4.2 ~ 16.7% relative shoot formation, respectively. During acclimatization, shoots were sampled every week and their total phenolic contents were analyzed. Among various growth factors, fresh weight showed the most dramatic increase from the 3rd week (88.0 mg/plant) to 4th week (132.7 mg/plant). Total phenolics and flavonoids contents were the highest at 1 st week of acclimatization. Depending on developmental stages, total phenolics and flavonoids contents were higher in 1-yr-old shoots grown ex vitro than in those of older field-grown or in vitro-grown plants. Amongst different ages of field grown plants, 6-year-old plants, the oldest in this study, showed the lowest content in total phenolics.
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