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박옥규,곽지나,정유정,김영호,홍현석,황병준,권승해,기윤 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.11
Precise 3D spatial mapping of cells and their connections within living tissues is required to fully understand developmental processes and neural activities. Zebrafish embryos are relatively small and optically transparent, making them the vertebrate model of choice for live in vivo imaging. However, embryonic brains cannot be imaged in their entirety by confocal or two-photon microscopy due to limitations in optical range and scanning speed. Here, we use light-sheet fluorescence microscopy to overcome these limitations and image the entire head of live transgenic zebrafish embryos. We simultaneously imaged cranial neurons and blood vessels during embryogenesis, generating comprehensive 3D maps that provide insight into the coordinated morphogenesis of the nervous system and vasculature during early development. In addition, blood cells circulating through the entire head, vagal and cardiac vasculature were also visualized at high resolution in a 3D movie. These data provide the foundation for the construction of a complete 4D atlas of zebrafish embryogenesis and neural activity.
各種添加物이 混合培養에서의 Salmonella 繁殖에 미치는 影響
朴玉圭 現代醫學社 1968 現代醫學 Vol.9 No.4
Amino acids, carbohydrates or vitamins were.added, respectively, into nutrient broth in which Salmonella typhi(I-1901) and Escherichia coli 0135 (0135) were inoculated, and growth response of 11901 to individual additives were studied. In mixed cultures, 0135 inhibited the growth of H901. The recovery of 1-1~01 was somewhat increased by cystine and methionine. Tryptophan had no effect on growth of 11901. However, h istidine and glutamic acid were inhibitory. Glucose and inositol increased the recovery of H901 at 5, 10, and 15 hr. after incubation,-whereas at 25 hr. the recovery of H901 was decreased to some extent. Lactose, mannose, ind PABA was inhibitory. Culture filtrates of 0135 inhibited the growth of 11901 and boiled filtrates for 30 min. almost restored the capacity of filtrates to support growth of 11901, indicating the presence of a, heat-labile inhibitor.