흰불나방 핵다각체병바이러스의 성상과 병원성에 관한 연구

임대준,현재선,백운하,임종성,Im Dae Joon,Hyun Jae Sun,Paik Woon Hah,Lim Jong Sung 한국응용곤충학회 1979 한국식물보호학회지 Vol.18 No.1

1975년 수원 잠업시험장에서 분리한 흰불나방 핵다각체 바이러스 성상과 령기, 화기별 및 병원체 보존조건에 따른 병원성을 검정하기 위하여 핵다각체 바이러스를 현탁액 및 건조시켜 실온$(18.5^{\circ}C)$, 냉장$(5^{\circ}C)$, 냉동$(-80^{\circ}C)$에 각각 보존, 이병사체는 그대로 양지 음와 지에 보존하였고 야외에서의 병원성, 가잠에 대한 교우감염을 검정한 결과 다음과 같다. 1. 흰불나방 핵다각체는 4면체와 6면체로 크기는 약 $2.0{\mu}$이고 바이러스 입자는 간상형의 $2\~12$본으로 $33m\mu\times35m\mu$이었다. 2. 6각형의 흰불나방 중장핵다각체 바이러스는 중장의 핵에서만 발견되었다. 3. 령기별 $LD_{50.}$ 2,3,4 및 5령에 대해 $8.377\times10^4,\;4.974\times10^5,\;2.621\times10^6$ 및 $9.471\times10^6PIBs/ml$ 였으며 $1.45\times10^6\;PIBs/ml$에 대한 $LT_{50}$은 각각 9.6, 11.5, 12.0 및 17.3일 이었다. 4. 핵다각체 바이러스는 1화기 유충보다 2화기 유충에서 감수성이 높았다. 5. 핵다각체 보존은 둔화건조하여 냉동 및 냉장보존하거나 이병사체를 음지에 보존하는 것이 바이러스 활성도의 감소가 적었다. 6. 야외에서의 효과적인 살포농도는 $6.4\times10^7\;PIBs/ml$이며 이의 $LT_{50}$은 3령은 4.8일, 5령은 14.2일이었다. 7. 흰불나방 핵다각체 바이러스와 가잠의 핵다각체 바이러스는 상호 교우감염을 일으키지 않았다. An inclusion forming virus isolated from a fan webworm, Hyphantria cunea, in 1975 was identified as a nuclear polyhedrosis virus. With the virus isolated in Korea, it was considered that the virus would be one of the valuable microorganism in microbial control. In this connection, 1) the shape and size of the virus for identification, 2) susceptibility of the various instar larvae to the virus, 3) the effects of storage condition on the pathogenicity and the cross infection of the virus to the larvae of Bombyx mori were examined. The results are summarized as follows; 1. The polyhedron was tetrahedron or hexahedron of $2\mu$ in size and the rod-shaped virus particles consisting of $2\~14$ rods in a bundle were $330m{\mu}\times35m{\mu}$ in size. 2. The hexagonal nuclear polyhedra were found only in the nucleus of the midgut cells but were variable in size. 3. The $LD_{50}$ values for the various instar larvae of H. cunea were $8.377\times10^4\;PIBs/ml$ for the second, $4.974\times10^5\;PIBs/ml$ for the fifth instar larvae. The $LT_{50}values$ for $10^6\;PIBs/ml$ were 9.6 days for the second, 11.5 days for the third, 12.0 days for the fourth and 17 days for the fifth instar larvae. 4. The susceptibility of H. cunea to the nuclear polyhedrosis virus was greater in the first generation than in the second generation. 5. The effect of the storage conditions on the pathogenicity of the nuclear polyhedra was less in refrigerator $(5^{\circ}C)$ and in freezing $(-80^{\circ}C)$ than in room temperature $(18.5^{\circ}C)$, especially as air-dried polyhedra than as suspension. The pathogenicity of the polyhedra seemed to decrease by sunlight during storage as cadavers, since rather greater decrease in pathogenicity was found in sunny condition than in shady condition. 6. The effective spray concentration was $6.4\times10^7\;PIBs/ml$ in the field and its $LT_50$ values for the third and the fifth instar larvae were 4.8 days and 14.2 days, respectively. 7. No cross infections were found in the nuclear polyhedrosis virus between H. cunea and B. mori. larvae.

뽕나무 가해(加害) 잎말이나방 류(類)에 관(關)한 조사(調査)

임대준 ( Dae Joon Im ),백현준 ( Hyun Jun Paik ) 한국잠사학회 1981 한국잠사곤충학회지 Vol.23 No.2

뽕나무를 가해하는 잎말이나방류의 종류와 우점종을 조사하기 위해 1979년부터 잠업시험장 포장에서 조사한 결과, 1. 뽕나무 가해잎말이나방류는 뽕나무 해충으로 미기록종인 뽕나무애기잎말이나방(가칭, Olethreutes hemiplaca MEYRICK)과 Olethreutes doubledayana BARRET을 포함 7종이 조사되었다. 2. 우리나라에서 우점종은 Olethreutes hemiplaca MEYRICK였으며 애모무늬잎말이나방(Adoxophyes orana F.&R.)이 그 다음이였다. This survey was carried out at the Sericultural Experiment Station. Suweon to clarify the kinds of leaf rollers damaging mulberry leaves and determine the dominant species of them. The results obtained are as follow: 1. A total of seven species including 2 unrecorded species, Olethreutes hemiplaca MAYRICK, and Olethreutes doubledayana BARRET, were found as mulberry attacking leaf rollers. 2. The dominant species of mulberry leaf rollers in Korea was identified as O. hemiplaca M. followed by Adoxophyes orana B.

콩 생식생장단계별 톱다리개미허리노린재(Riptortus clavatus)흡즙에 의한 콩 종실 피해

정진교,윤종탁,임대준,박종호,김욱한,Jung, Jin-Kyo,Youn, Jong-Tag,Im, Dae-Joon,Park, Jong-Ho,Kim, Uk-Han 한국응용곤충학회 2005 한국응용곤충학회지 Vol.44 No.4

콩 생식생장 단계별로 톱다리개미허리노린재를 접종하거나, 침으로 꼬투리를 치르는 인위적 가해를 통해 콩 종자 피해를 조사하였다. 착협기에 톱다리개미허리노리재 성충을 섭식시켰을 때, 빈 꼬투리와 종자로 전혀 발육하지 못한 판형종자의 생성비율이 종실비대성기에서의 가해보다 높은 것이 관찰되었다. 종실비대성기에 섭식시켰을 때는 종자모양은 거의 정상적이나 종피에 피해흔적이 뚜렷한 종자 수가 유의적으로 증가하였다. 종자모양이 많이 변형되거나 불완전하게 성숙한 기형종자의 비율은 처리 사이에 차이가 없었고 다른 종자형태들에 비해 상대적으로 낮은 비율을 차지하였다 착협시 피해에서 생성된 판 종자의 83%, 종실비대성기에 생성된 피해흔적이 있는 종자의 91%에서 종자 표면 위에 실험곤충의 빨대피막이 관찰되었다. 개화기 섭식은 피해종자 생성에 거의 영향을 주지않았다. 종실비대성기와 성숙기에 침을 이용하여 콩 꼬투리를 찔러 물리적인 가해를 하였을 때, 수확기에 건전한 종자들을 발견할 수 없었고, 피해gms적이 있는 종자의 비율이 기형종자 보다 많았다. 종실비대성기에 침으로 찌른 회수가 많은 경우 판형종자와 기형종자의 비율이 증가하였고, 피해흔적 종자 비율은 상대적으로 감소하였는데 무게감소 비율은 적게 찌른 경우 보다 상대적으로 켰다. 그러나 성숙기 인위적 가해에서는 수확종자의 무게감소가 나타나지 않았다. Soybean seed injury was analyzed in the experiments that soybean pods were allowed to be sucked by adults of the bean bug, R. clavatus, and were picked with a specimen pin. While attack by the bean bug at podding stage of soybean caused the increase of empty pods and completely-undeveloped seeds, attack at full seed stage caused the increase of seeds wi distinct injury mark on seed-coat. The ratio of deformed seed was lower than those of injury-marked seed and undeveloped seed when attacked during all stages. In at full bloom stage hardly produced injury-marked seeds and deformed seeds. When the injured seeds were dyed with acid-fuchsine solution, stylet sheaths of R. clavatus formed on seed-coat were observed in 83% of undeveloped seeds formed under attack during podding stage and in 91% of injury-marked seeds formed during full seed stage. In pods injured with a specimen pin at full seed and full maturity stages, no healthy seeds could be obtained from the pods. And the more picked at full seed stage, the more deformed seeds were produced and the higher weight reduction of injury-marked seed occurred. However, pin-injury at full maturity stage didn't give rise to weight reduction of seeds.

조명나방(Ostrinia furnacalis) 알에 대한 쌀좀알벌(T. evanescens)과 T. ostriniae의 기생특성

정진교,박종호,임대준,한태만,Jung Jin Kyo,Park Jong Ho,Im Dae Joon,Han Tae Man 한국응용곤충학회 2005 한국응용곤충학회지 Vol.44 No.1

Parasitism of the egg parasitoid, Trichogramma evanescens, to its main host insect, the Asian corn borer, Ostrinia furnacalis in Korea was compared with that of T. ostriniae that is the dominant species in China on the same host insect. Parasitoid adults of both species emerged more than 50 percent within 4 hours after lights-on in 16L/8D photo period regime and showed a circadian rhythm of emergence. The developmental period from oviposition to emergence in both parasitoids was ca. 11 days and there were no significant differences between the two species and between female and male of each species, either. Both species also showed superparasitism even when the parasitic rates in one egg mass were below 100 percent. Both species oviposited by 5 days after emergence, and maximum longevities of each female adult of both species were 8 day for T. evanescens and 6 day for T. ostriniae. The total number of eggs parasitized by T. evanescens was ca. 38 eggs and ca. 31 eggs by T. ostriniae. Newly emerged female parasitoid laid eggs on about $50\%$ of the host insect egg mass, and the parasitism decreased with the adult age of egg parasitoids in both species. The sex ratio of two species was female-biased about $80\%$. 조명나방(Ostrinia furnacalis Guenee)의 국내 우점 알기생벌인 쌀좀알벌(T. evanescens)과 같은 곤충을 숙주로 삼는 T. ostriniae의 기생특성을 조명나방 알을 이용하여 비교하였다. 일정한 사육조건 ($25^{\circ}C$, 16L/8D)에서 두 알기생벌은 명조건 후 4시간 안에 $50\%$ 이상 우화하는 일일주기성을 보였다. 산란에서 우화까지의 발육기간은 두 종 약 11일 정도였고, 두 종 모두 종간 및 종내 암수 간에 차이가 없었으며, 알기생율이 $100\%$ 미만임에도 불구하고 과기생을 보였다. 두 종 모두 암컷은 우화 후 5일째까지 산란하였고, 수명은 쌀좀알벌에서 최대 8일이었고, T. ostriniae에서 6일이었다. 조명나방의 기생알 수는 각 각 약 38개와 31개였는데, 두 종 모두에서 우화 첫째날 알기생벌이 산란한 알 수가 전 산란기간 동안 산란한 알 수의 약 $50\%$를 차지하였다. 성비는 두 종 모두 암컷이 약 $80\%$를 차지하는 암컷편향적이었다.

온도, 광, GA₃ 및 저장방법이 참당귀 발아에 미치는 영향

유홍섭(Hong Seob Yu),강병화(Byeung Hoa Kang),임대준(Dae Joon Im),김충국(Chung Guk Kim),김영국(Young Guk Kim),이승택(Seoung Tack Lee),장영희(Yeong Hee Chang) 한국약용작물학회 1995 한국약용작물학회지 Vol.3 No.1

Theses studies were conducted to clarify the germinating characteristics of seed treated with different temperature, light, and Gibberellin(GA₃) to produce the homogeneous seedlings Angelicagigas NAKAI. The results obtained are summarized as follows : Rate and speed of germinating at 25℃ were higher than 15℃ and 20℃ by 87.3% and 68.9% and also the required day for germination at 25℃ was shortest by 14.1 days. Germination rate at the light condition was 90.7% and that at the dark was 65.7%. Germinating ability of seed stored at 4℃ and -10℃ for 165 days as dried states was 92.0% and 91.3% while that at room temperature was 50.3%, but the longer storage of seed than 165 days decreased the ability. Gibberellin treatment by 10ppm to seed stored at 4℃ and by 6ppm to seed stored at -10℃ increased the germination rate more by 20.9% and 31.7% than nontreated seed.

미강추출물을 이용한 곤충병원성 곰팡이 Beauveria bassiana의 최적 배양조건 및 효소활성

김창수(Chang-Su Kim),이중복(Jung-Bok Lee),김범수(Beam-Soo Kim),이민혜(Min-Hye Lee),강경묵(Kyeong-Muk Kang),주우홍(Woo-Hong Joo),김진원(Jin-Won Kim),임대준(Dae-Joon Im),권기석(Gi-Seok Kwon) 한국생명과학회 2013 생명과학회지 Vol.23 No.8

본 연구에서는 온실가루이 방제를 위하여 감염된 이병체로부터 균주 분리 및 농촌진흥청 및 경상북도농업기술원에서 균주를 분양 받아 5 종의 균주를 확보하여, 최종적으로 활성이 가장 높은 분리균주 M130을 선발하였다. Beauveria bassiana M130을 이용하여 균주가 갖는 효소학적 활성을 분석하였다. Chitinase와 protease의 활성을 조사한 결과, 타 균주에 비하여 높은 효소학적 활성을 가지는 조건을 얻었으며, 선발된 균주의 포자생산의 최적화를 위한 방안으로 PDB (potato dextrose broth)배지와 미강추출물을 이용한 ERBM (extracted rice bran medium)을 비교하여, 더 높은 생육도와 포자 생성량을 보인 ERBM을 본 균주를 배양하는 기본배지로 선발하였다. ERBM을 이용한 액체배양시의 최적조건으로, 기본배지인 ERBM에 0.5% (NH₄)₂SO₄를 첨가하여 pH 5, 28℃에서 배양최적화를 하였다. 향후 곤충병원성곰팡이 Beauveria bassiana M130의 최적화 조건에 따라 배양을 한다면 포자의 생산량 및 방제효과가 높아질 것으로 사료된다. The greenhouse whitefly, Bemisia tabaci, is considered one of the most destructive pests of crops. In this study, we aimed to determine the optimal liquid culture conditions in shake flasks for maximal sporulation of Beauveria bassiana M130 using rice bran. The optimal initial pH for the spore production of B. bassiana using extracted rice bran medium was 5.2 and 28℃. The screening in shake flasks of carbon and nitrogen sources resulted in the identification of an optimal medium based on 0.5% (NH₄)₂SO₄, with extracted rice bran 8:1. Using this medium, a production level of 2.15×10<SUP>9</SUP> spores per ml was obtained after six days from culture inoculation at 28℃ in a rotary shaking incubator at 130 rpm. In addition, the specific activities of extracellular enzymes of chitinase and protease were 4,296μmol and 375 μmol, respectively. These results suggest that Beauveria bassiana M130 could be a biocontroller for the greenhouse whitefly.

한국산 천적 곤충 목록

박규택,백종철,임대준,백운하,최광렬 서울大學校 農科大學 1981 서울대농학연구지 Vol.6 No.2