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SHIN, PYONG KYUN,Koo, Ja Hyup,Lee, Woo Jong,SEO, JIN HO 한국화학공학회 1996 Korean Journal of Chemical Engineering Vol.13 No.1
A mathematical model was formulated to describe growth and cloned protein production in the recombinant Escherichia coli cells containing phoA-directed expression systems. Kinetic parameters for the strains with two fusion genes (phoA-lacZ either on the chromosome or on a multicopy plasmid and phoA-amyE on a multicopy plasmid) were estimated and compared to analyze the effects of cloning site (chromosome and plasmid), product type (E. coli β-galactosidase and Bacillus subtilis α-amylase), and culture temperature on the cell's behavior. The presence of a multicopy plasmid reduced the specific growth rate and the phosphate uptake rate of the cell, both by 10%, compared with those of the chromosome-integrated strain. The overexpression of B. subtilis α-amylase decreased the specific growth rate and the glucose consumption rate more than the β-galactosidase overproduction system. The presence of multiple copies of the phoA promoter on either an intact phoA gene or the fusion gene reduced both the repression and derepression efficiencies. Culture temperatures showed a significant effect on α-amylase production. A temperature of 30℃ is more desirable than 37℃ for α-amylase production in the recombinant E. coli containing the phoA promoter.
고분자의 혐기적 생분해도에 영향을 미치는 하수 소화 슬러지의 인자들
김명희,신평균,김종민,김병홍 한국화학공학회 1996 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.34 No.1
형기적 조건에서 플라스틱의 생분해도를 측정할 때 미생물원으로 사용되는 소화조 슬러지의 특성 중 플라스틱의 생분해도에 미치는 영향 인자에 대하여 조사하였다. 1994년 5월부터 7개월간 채취한 중랑 하수처리장(서울) 소화 슬러지의 유기물 함량(total organic solids)은 0.52-0.86%로 변하였다. 슬러지의 cellophane에 대한 생분해도는 슬러지의 유기물 함량과 일치하지 않았으며 여름철(7, 9월)에 채취한 슬러지의 생분해 활성이 특히 낮았다. 그러나 poly-β-hydroxybuty-rate/hydroxyvalerate 공중합체(PHB/HV, 92/8, W/W)의 생분해도는 슬러지의 유기물 함량과 일치하였다. 슬러지의 접종량이 증가할수록 cellophane과 PHB/HV의 생분해 속도는 증가하였고 분해 지체기는 감소하였다. 슬러지 접종의 최적농도는 배지 중의 총 유기성 고체의 농도 0.10-0.20%(w/v)이였다. The characteristics of anaerobic digested sludge affecting the biodegradation of plastic materials under anaerobic condition were studied. The percent organic matter(total solid×volatile solid, VSS) of sludges obtained from Joongrang Municipal Wastewater Treatment Plant in Seoul had varied from 0.52% to 0.86% during the period of 7 months from May, 1994. The biodegradation activity of sludges for cellophane didn't correspond to the VSS. Sludges of July and September showed lower activities than others. But biodegradation activity for poly-β-hydroxybutyrate/hydroxyvalerate copolymer(PHB/HV, 92/8, W/W) corresponded well to VSS value. The biodegradation rate for both cellophane and PHB/HV increased as the concentration of sludge inoculum increases along with the decrease of lag period. The optimal total organic solids in the test medium was determined as 0.10-0.20%(W/V).
SEO, JIN HO,SHIN, PYONG KYUN,Koo, Ja Hyup,Lee, Woo Jong 한국화학공학회 1996 NICE Vol.14 No.3
A mathematical model was formulated to describe groarth and cloned protein production in the recombinant Escherichia coli cells containing pho-directed expression systems. Kinetic parameters for the strains with two fusion genes phoA-lacZ either on the chromosome or an a multicopy plasmid and phoA-amyE on a multicopy plasmid were estimated and compared to analyze the effects of cloning site (chromosome md plasmid), product type (E, coli β-galactosidase and Bacillus subrilis α-amylase), and culture temperature on the cell's behavior. The presence of a multiatpy plasmid reduced the specific growth rate and the phosphate uptake rate of the cell, both by 10%, compared with those of the chromosome-integrated strain. The overexpression of B. suhtilis α-amylase decreased the specific growth rate and the glucose consumption rate more than the β-galactosidase overproduction system. The presence of multiple copies of the phoA promoter on either an intact phoA gene or the fusion gene reduced both the repression and derepression efficiencies. Culture temperatures showed a significant effect on α-amylase production. A temperature of 30℃ is more desirable than 37℃ for α-amylase production in the recombinant E. coli containing the phoA promoter.
Effects of compost and culture conditions on the biodegradation of degradable plastics
Jung, Eun Joo,Shin, Pyong Kyun,Bae, Hee Kyung 嶺南大學校 環境問題硏究所 1998 環境硏究 Vol.18 No.1
AbstractBiodegradable polymers have been regarded as a good alternative to solve the solid waste problem caused by non-degradable synthetic polymers such as polyethylene and polystyrene. Because of their biodegradability, they can be easily treated in the current solidwaste treatment systems like composting or anaerobic digestion. To promote the usage of biodegradable polymers, proper evaluation methods are critical. Many standard methods have been issued for biodegradable polymers. One of the methods is the test method to measure the biodegradability under controlled composting condition.To evaluate the effectiveness of current test method under controlled composting condition, the effects of compost conditions on the biodegradability of degradable polymers were studied with compost harvested after thermophilic degradation stage or cured for one month at two different temperatures, 55 and 15℃. Compost harvest right after thermophilic degradation stage showed highest biodegradation activities among three composts for both a synthetic aliphatic polyester and a natural polymer, cellulose at test temperature of 55℃. When the biodegradability test with the compost cured at ambient temperature was performed at the temperatures of 35 and 55℃, cellulose degradation was faster at 35℃ than at 55℃, while the degradations of the syntheitic aliphatic polyester at both temperatures were similar. Our results reveal that the biodegradation activity of the compost is greatly influenced by the test temperature and compost conditions.
Effect of pH on Phase Separated Anaerobic Digestion
Jung, Jin-Young,Lee, Sang-Min,Shin, Pyong-Kyun,Chung, Yun-Chul The Korean Society for Biotechnology and Bioengine 2000 Biotechnology and Bioprocess Engineering Vol.5 No.6
A pilot scale experiment was performed for a year to develop a two-phase anaerobic process for piggery wastewater treatment (COD: 6,000mg/L, BOD: 4,000mg/L, SS: 500mg/L, pH 8.4, alkalinity 6,000mg/L). The acidogenic reactor had a total volume of 3㎥, and the methanogenic reactor, an anaerobic up-flow sludge filter, combining a filter and a sludge bed, was also of total volume 3㎥(1.5㎥ of upper packing material). Temperatures of the acidogenic and methanogenic reactors kept at 20$^{\circ}C$ and 35$^{\circ}C$, respectively. When the pH of the acidogenic reactor was controlled at 6.0-7.0 with HCl, the COD removal efficiency increased from 50 to 80% over a period of six months, and as a result, the COD of the final effluent fell in the range of 1,000-1,500 mg/L. BOD removal efficiency over the same period was above 90%, and 300 to 400 mg/L was maintained in the final effluent. The average SS in the final effluent was 270 mg/L. The methane production was 0.32㎥ CH$_4$/kg COD(sub)removed and methane content of the methanogenic reactor was high value at 80-90%. When the pH of the acidogenic reactor was not controlled over the final two months, the pH reached 8.2 and acid conversion decreased compared with that of pH controlled, while COD removal was similar to the pH controlled operation. Without pH control, the methane content in the gas from methanogenic reactor improved to 90%, compared to 80% with pH control.