http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Ramiro Guerrero-Santos,Gabriela Cabrales-Orona,John Paul Délano-Frier,Judith Cabello-Romero,José Román Torres-Lubián,José Humberto Valenzuela-Soto 한국식물병리학회 2024 Plant Pathology Journal Vol.40 No.1
Huanglongbing (HLB) is a disease caused by the phloem- limited Candidatus Liberibacter asiaticus (CLas) that affects the citrus industry worldwide. To date, only indirect strategies have been implemented to eradicate HLB. Included among these is the population control of the psyllid vector (Diaphorina citri), which usually provides inconsistent results. Even though strategies for direct CLas suppression seem a priori more promising, only a handful of reports have been focused on a confrontation of the pathogen. Recent developments in polymer chemistry have allowed the design of polycationic self-assembled block copolymers with outstanding antibacterial capabilities. Here, we report the use of polymeric nano-sized bactericide particles (PNB) to control CLas directly in the phloem vasculature. The field experiments were performed in Rioverde, San Luis Potosí, and is one of the most important citrusproducing regions in Mexico. An average 52% reduction in the bacterial population was produced when PNB was injected directly into the trunk of 20 infected trees, although, in some cases, reduction levels reached 97%. These results position PNB as a novel and promising nanotechnological tool for citrus crop protection against CLas and other related pathogens.
Impact of Zinc Stress on Biochemical and Biophysical Parameters in Coffea Arabica Seedlings
Jacqueline Oliveira dos Santos,Cinthia Aparecida Andrade,Kamila Rezende Dázio de Souza,Meline de Oliveira Santos,Isabel Rodrigues Brandão,Jose Donizeti Alves,Iasminy Silva Santos 한국작물학회 2019 Journal of crop science and biotechnology Vol.22 No.3
Zinc is an essential micronutrient for the healthy development of plants, since its insufficient and supraoptimal doses can disrupt the metabolism and biomass production. We aimed to investigate the physiological responses of coffee seedlings to Zn deficiency and excess. Six-month-old seedlings were transferred to plastic pots containing a nutrient solution. The treatments were control (0.03 ppm), zinc deficiency (0.00 ppm), and zinc excess (0.12 ppm). The evaluations were performed in leaves and roots at the beginning of the treatments and after 30 and 60 d of treatments. Zn deficiency and excess increased the production of hydrogen peroxide, antioxidant enzymes activity, ascorbate, and lipid peroxidation contents. The imbalance in zinc nutrition reduced total chlorophyll content and increased carotenoids content throughout the experimental period. Lower biomass and proline accumulation were observed only for deficient seedlings at the end of the experiment. The characteristics analyzed showed that zinc deficiency caused greater damage to the Coffea arabica plants of (Catuai cultivar) than zinc excess.
Bárbara Jaime dos Santos,Débora Balabram,Virginia Mara Reis Gomes,Carolina Costa Café de Castro,Paulo Henrique Costa Diniz,Marcelo Araújo Buzelin,Cristiana Buzelin Nunes 대한암학회 2024 Cancer Research and Treatment Vol.56 No.1
Purpose Neoadjuvant chemotherapy (NACT) can change invasive breast carcinomas (IBC) and influence the patients’ overall survival time (OS). We aimed to identify IBC changes after NACT and their association with OS.Materials and Methods IBC data in pre- and post-NACT samples of 86 patients were evaluated and associated with OS.Results Post-NACT tumors changed nuclear pleomorphism score (p=0.025); mitotic count (p=0.002); % of tumor-infiltrating inflammatory cells (p=0.016); presence of <i>in situ</i> carcinoma (p=0.001) and lymphovascular invasion (LVI; p=0.002); expression of estrogen (p=0.003), progesterone receptors (PR; p=0.019), and Ki67 (p=0.003). Immunohistochemical (IHC) profile changed in 26 tumors (30.2%, p=0.050). Higher risk of death was significatively associated with initial tumor histological grade III (hazard ratio [HR], 2.94), high nuclear pleomorphism (HR, 2.53), high Ki67 index (HR, 2.47), post-NACT presence of LVI (HR, 1.90), luminal B–like profile (HR, 2.58), pre- (HR, 2.26) and post-NACT intermediate mitotic count (HR, 2.12), pre- (HR, 4.45) and post-NACT triple-negative IHC profile (HR, 4.52). On the other hand, lower risk of death was significative associated with pre- (HR, 0.35) and post-NACT (HR, 0.39) estrogen receptor–positive, and pre- (HR, 0.37) and post-NACT (HR, 0.57) PR-positive. Changes in IHC profile were associated with longer OS (p=0.050). In multivariate analysis, pre-NACT grade III tumors and pre-NACT and post-NACT triple negative IHC profile proved to be independent factors for shorter OS.Conclusion NACT can change tumor characteristics and biomarkers and impact on OS; therefore, they should be reassessed on residual samples to improve therapeutic decisions.
Sandra Castillo,Jorge Dávila-Aviña,Norma Heredia,Santos Garcia 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.2
Adherence and invasion to cells are the key processes during infection development by Campylobacter jejuni (C. jejuni). In this study, extracts from the byproducts of Citrus limon, Citrus aurantium, and Citrus medica were added to the cultures of C. jejuni, and the adherence and invasion of C. jejuni to HeLa cells and the expression of cadF and ciaB genes were analyzed. The relative expression of the genes was determined by quantitative reverse transcription PCR (qRT-PCR). The antioxidant activity was determined using spectrophotometric methods. Byproduct extracts at subinhibitory concentrations affected the adherence (reduced 2.3 to 99%) and invasion (reduced 71.3 to 99.2%) to the HeLa cells. The expression of cadF and ciaB was reduced from 66 to 99% and from 81 to 99%, respectively. The total phenolic content of the byproducts varied from 92 to 26 mg GAE/g and the total flavonoids varied from 161 to 29.29 mg QE/g. C. aurantium showed the highest percentage of radical scavenging activity (RSA, 90.1). These extracts can prove as effective alternatives for devising new strategies to control Campylobacter infections.
Dye-Perfused Human Placenta for Simulation in a Microsurgery Laboratory for Plastic Surgeons
Zambrano-Jerez Laura C.,Díaz-Santamaría Karen D.,Rodríguez-Santos María A.,Alarcón-Ariza Diego F.,Meléndez-Flórez Genny L.,Ramírez-Blanco Mónica A. 대한성형외과학회 2023 Archives of Plastic Surgery Vol.50 No.6
In recent decades, a number of simulation models for microsurgical training have been published. The human placenta has received extensive validation in microneurosurgery and is a useful instrument to facilitate learning in microvascular repair techniques as an alternative to using live animals. This study uses a straightforward, step-by-step procedure for instructing the creation of simulators with dynamic flow to characterize the placental vascular tree and assess its relevance for plastic surgery departments. Measurements of the placental vasculature and morphological characterization of 18 placentas were made. After the model was used in a basic microsurgery training laboratory session, a survey was given to nine plastic surgery residents, two microsurgeons, and one hand surgeon. In all divisions, venous diameters were larger than arterial diameters, with minimum diameters of 0.8 and 0.6 mm, respectively. The majority of the participants considered that the model faithfully reproduces a real microsurgical scenario; the consistency of the vessels and their dissection are similar in in vivo tissue. Furthermore, all the participants considered that this model could improve their surgical technique and would propose it for microsurgical training. As some of the model's disadvantages, an abundantly thick adventitia, a thin tunica media, and higher adherence to the underlying tissue were identified. The color-perfused placenta is an excellent tool for microsurgical training in plastic surgery. It can faithfully reproduce a microsurgical scenario, offering an abundance of vasculature with varying sizes similar to tissue in vivo, enhancing technical proficiency, and lowering patient error.
Priscila Vaz de Arruda,Júlio César dos Santos,Rita de Cássia Lacerda Brambilla Rodrigues,Débora Danielle Virgínio da Silva,Celina Kiyomi Yamakawa,George Jackson de Moraes Rocha,Jonas Nolasco Júnior,Jo 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.47 No.-
In this study, volumetric oxygen mass transfer coefficient (kLa) was selected as a criterion for facilitatingthe scale up of xylitol production by Candida guilliermondii at the bench and pilot-scale level. A kLa valueof 16 h 1 was applied in reactors with volumetric capacity of 2.4 L, 18 L and 125 L. Fermentation wassuccessfully scaled-up from the bench to pilot-scale level with all experiments demonstrating aminimum of 60% xylose to xylitol conversion efficiency. Under all evaluated conditions glycerol andethanol were also produced as by-products of xylose metabolism. Only minor differences were observedin the fermentation profile when reactor volumes ranging from 2.4 L to 125 L were used forexperimentation purposes, reaching, at pilot scale, yield and volumetric productivity of 0.55 g g 1and 0.31 g L 1 h 1, respectively, with maximum specific growth rate of 0.26 h 1. This demonstrates andreinforces the feasibility of using kLa as scale up criterion. The use of this parameter allowed precisereproduction of results obtained at bench bioreactor level to a larger scale; this is extremely crucial andimportant information considering that the aim of the proposed biotechnological process is to reach thelevel required for the industrial viability.
Handray Fernandes de Souza,Giovana Felicio Monteiro,Victor Dédalo Di Próspero Gonçalves,João Vitor dos Santos,Amanda Cristina Dias de Oliveira,Karina Nascimento Pereira,Mariana Fronja Carosia,Marina V 한국식품과학회 2024 Food Science and Biotechnology Vol.33 No.7
Mead is a fermented alcoholic beverage produced by yeast action on a diluted solution of honey. In this study, for the first time, sensory acceptance, purchase intention and color parameters of potentially probiotic mead with Saccharomyces boulardii were evaluated. The mead with S. boulardii presented yeast counts higher than 106 CFU/mL, being considered potentially probiotic, and tended to be yellow in color. About 160 tasters participated in the sensory evaluation, and 69.38% knew mead, but only 35.62% had tried the beverage. In terms of acceptance, the mead were within the acceptable range (above 5), and F2 (with initial soluble solids of 30° Brix and S. boulardii concentration of 0.030 g/L) was the most accepted, with an overall average of 7.63 ± 1.42 on the nine-point hedonic scale. In addition, F2 presented the highest purchase intention. In conclusion, the mead showed a tendency towards the color yellow and good sensory acceptance.
Bursectomy, Curettage, and Chemotherapy in Tuberculous Trochanteric Bursitis
Luis R. Ramos-Pascua,José A. Carro-Fernández,José A. Santos-Sánchez,Paula Casas Ramos,Luis J. Díez-Romero,Francisco M. Izquierdo-García 대한정형외과학회 2016 Clinics in Orthopedic Surgery Vol.8 No.1
We presented three patients with trochanteric tuberculosis and described the clinical and imaging findings of the infection. Histology revealed a necrotizing granulomatous bursitis and microbiology confirmed tuberculosis. All cases were successfully treated with bursectomy and curettage of the trochanteric lesion and antituberculous chemotherapy including isoniazid, pyrazinamide, rifampicin, and ethambutol.
Leandro Fernandes Andrade,Gleika Larisse Oliveira Dorasio de Souza,Silvia Nietsche,Adelica Aparecida Xavier,Marcia Regina Costa,Acleide Maria Santos Cardoso,Marlon Cristian Toledo Pereira,Débora Franc 한국미생물학회 2014 The journal of microbiology Vol.52 No.1
A total of 40 endophytic bacterial isolates obtained from banana tree roots were characterized for their biotechnological potential for promoting banana tree growth. All isolates had at least one positive feature. Twenty isolates were likely diazotrophs and formed pellicles in nitrogen-free culture medium, and 67% of these isolates belonged to the genus Bacillus sp. The isolates EB-04, EB-169, EB-64, and EB-144had N fixation abilities as measured by the Kjeldahl method and by an acetylene reduction activity assay. Among the 40isolates, 37.5% were capable of solubilizing inorganic phosphate and the isolates EB-47 and EB-64 showed the highest solubilization capacity. The isolate EB-53 (Lysinibacillus sp.)had a high solubilization index, whereas 73% of the isolates had low solubilization indices. The synthesis of indole-3-acetic acid (IAA) in the presence of L-tryptophan was detected in 40% of the isolates. The isolate EB-40 (Bacillus sp.)produced the highest amount of IAA (47.88 μg/ml) in medium supplemented with L-tryptophan and was able to synthesize IAA in the absence of L-tryptophan. The isolates EB-126 (Bacillus subtilis) and EB-47 (Bacillus sp.) were able to simultaneously fix nitrogen, solubilize phosphate and produce IAA in vitro. The results of this study demonstrated that the isolates analyzed here had diverse abilities and all have the potential to be used as growth-promoting microbial inoculants for banana trees.
J-PLUS: Identification of low-metallicity stars with artificial neural networks using SPHINX
Whitten, D. D.,Placco, V. M.,Beers, T. C.,Chies-Santos, A. L.,Bonatto, C.,Varela, J.,Cristó,bal-Hornillos, D.,Ederoclite, A.,Masseron, T.,Lee, Y. S.,Akras, S.,Borges Fernandes, M.,Caballero, J. Springer-Verlag 2019 Astronomy and astrophysics Vol.622 No.-
<P><I>Context.</I> We present a new methodology for the estimation of stellar atmospheric parameters from narrow- and intermediate-band photometry of the Javalambre Photometric Local Universe Survey (J-PLUS), and propose a method for target pre-selection of low-metallicity stars for follow-up spectroscopic studies. Photometric metallicity estimates for stars in the globular cluster M15 are determined using this method.</P><P><I>Aims.</I> By development of a neural-network-based photometry pipeline, we aim to produce estimates of effective temperature, <I>T</I>eff, and metallicity, [Fe/H], for a large subset of stars in the J-PLUS footprint.</P><P><I>Methods.</I> The Stellar Photometric Index Network Explorer, SPHINX, was developed to produce estimates of <I>T</I>eff and [Fe/H], after training on a combination of J-PLUS photometric inputs and synthetic magnitudes computed for medium-resolution (<I>R</I> ~ 2000) spectra of the Sloan Digital Sky Survey. This methodology was applied to J-PLUS photometry of the globular cluster M15.</P><P><I>Results.</I> Effective temperature estimates made with J-PLUS Early Data Release photometry exhibit low scatter, σ(<I>T</I>eff) = 91 K, over the temperature range 4500 < <I>T</I>eff (K) < 8500. For stars from the J-PLUS First Data Release with 4500 < <I>T</I>eff (K) < 6200, 85 ± 3% of stars known to have [Fe/H] < −2.0 are recovered by SPHINX. A mean metallicity of [Fe/H] = − 2.32 ± 0.01, with a residual spread of 0.3 dex, is determined for M15 using J-PLUS photometry of 664 likely cluster members.</P><P><I>Conclusions.</I> We confirm the performance of SPHINX within the ranges specified, and verify its utility as a stand-alone tool for photometric estimation of effective temperature and metallicity, and for pre-selection of metal-poor spectroscopic targets.</P>