Research on Thymopentin Loaded Oral N-Trimethyl Chitosan Nanoparticles

Yuan, Xiao-Jia,Zhang, Zhi-Rong,Song, Qing-Guo,He, Qin The Pharmaceutical Society of Korea 2006 Archives of Pharmacal Research Vol.29 No.9

Peptides, although high efficacy and specificity in their physiological function, usually have low therapeutical activities due to their poor bioavailability when administrated orally. Nanoparticles have been regarded as a useful vector for targeted drug delivery system because they can protect drug from being degraded quickly and pass the gastrointestinal barriers. Here we described a novel oral N-trimethyl chitosan nanoparticles formulation containing thymopentin (Tp5-TMC-NP). N-trimethyl chitosan (TMC) was synthesized and then used to prepare Tp5-TMC-NP by ionotropic gelation. A three-factor, five-level CCD (Central Composite Design) design was used in the optimization procedure, with HPLC as the analyzing method. The resulting Tp5-TMC-NP had a regular spherical surface and a narrow particle size range with a mean diameter of 110.6 nm. The average entrapment efficiency was 78.8%. The lyophilized Tp5-TMC-NP formulation was stable in $4^{\circ}C\;or\;-20^{\circ}C$ after storage of 3 months without obvious changes in morphology, particle size, pH and entrapment ratio. The results of the flow cytometer determination showed that the ratio of $CD4^+/CD8^+$ of Wistar female rat given Tp5-TMC-NP (ig) was 2.59 time that of the group given Tp5 (ig).

A New Microtiter Plate-based Screening Method for Microorganisms Producing Alpha-amylase Inhibitors

Zhi-Hua Feng,Yuan-Shan Wang,Yu-Guo Zheng 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.5

Alpha-amylase inhibitors are widely used by the pharmaceutical and agricultural industries, such as the treatment of diabetes and obesity and insect controller. Here, we developed a colorimetric method to screen for α-amylase inhibitor producing strains or mutants with higher α-amylase inhibitor productivity. This method relies on absorbance changes at 402 nm that are due to the inhibition of α-amylase catalyzed hydrolysis of 2-Chloro-4-nitrophenyl-4-O-β-D-galactopyranosyl-maltoside by α-amylase inhibitors. The assay can be performed on a microtiter plate, making it simple and convenient. Using this method,α-amylase inhibitor producing strains and mutants with higher α-amylase inhibitor productivity can be rapidly screened. One strain, ZJB-08196, with the highest α-amylase inhibition was isolated and identified as Actinoplanes utahensis, and one mutant with higher acarbose production was obtained by screening 3,000 variants using this method.

Insect-Proof Nets Affect Paddy Field Microclimate Parameters and Grain Quality of Different Japonica Rice Varieties

Zhi Guo,Hongjiang Liu,Jianchu Zheng,Liugen Chen,Haiyan Yuan 한국작물학회 2015 Journal of crop science and biotechnology Vol.18 No.2

Insect-proof nets (IPN) are widely used to protect crops from invertebrate pests. The effects of IPN on the main microclimate parameters of paddy fields, as well as grain quality, were investigated for three japonica Oryza sativa L. varieties (Nanjing 44, Nanjing 45, and Suxiangjing 3) that are widely grown in China. The environmental temperature of IPN-treated fields increased slightly, whereas the maximum value of photosynthetically active radiation (PAR), daily cumulative PAR, and daily cumulative global radiation all decreased. However, the ratio of PAR to global radiation increased significantly, from 15.08 to 45.48%. Compared to the no insect-proof net (NIPN) treatment, the head rice percentage increased significantly for all three varieties; however, the chalky grain percentage, area and degree, and the amylose content all decreased significantly. The response of gel consistency, protein concentration, and the main parameters of the rapid visco analyzer (RVA) profile of rice starch to IPN treatment differed between varieties. The peak viscosity and breakdown were increased compared to the NIPN treatment, whereas the final viscosity and setback were decreased. However, no significant differences were found between the IPN and NIPN treatments. Furthermore, statistical analysis indicated no interaction between rice planting pattern (RPP) and rice variety (RV) for the rice grain quality parameters. These results suggest that IPN treatment improved rice grain quality for all japonica rice varieties studied, possibly due to the slight increase in environmental temperature and increased ratio of PAR to global radiation in IPN treated paddy fields.

Extramedullary Plasmacytoma Involving the Bilateral Adrenal Glands on MR Imaging

Yuan Li,Ying-kun Guo,Zhi-gang Yang,En-sen Ma,Peng-qiu Min 대한영상의학회 2007 Korean Journal of Radiology Vol.8 No.3

We report here on a 64-year-old woman with extramedullary plasmacytoma involving the bilateral adrenal glands. Primary adrenal extramedullary plasmacytoma is extremely rare and only three cases of extramedullary plasmacytoma in the unilateral adrenal gland have currently been reported on. This case is of interest in that the bilateral adrenals were involved. In this article, we present the MRI findings and we briefly review the relevant literature.

Treatment Efficacy and Prognostic Factors for Huge HCC Based on Barcelona Clinic Liver Cancer Staging

Zhang, Zhi-Ming,Zhang, Yu-Mei,Gao, Sheng,Yuan, Wei-Ping,Zhao, Yin-Nong,Xiang, Bang-De,Wu, Fei-Xiang,Wu, Guo-Bin,Liu, Jian-Yong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.20

Objective: To explore the most appropriate treatment for patients with hepatocellular cancer (HCC) >10 cm by using the Barcelona Clinic Liver Cancer (BCLC) classification. Materials and Methods: A total of 124 HCC patients undergoing surgery were selected. Disease-free survival (DFS), overall survival (OS) and prognostic factors were respectively assessed. Results: This study showed that the cumulative 1-, 3-, 5-year survival rates were 79.7%, 59.8% and 41.6% in BCLC-A patients, 76.2%, 9.5% and 0% in BCLC-B patients and 44.9%, 0% and 0% in BCLC-C patients, respectively. The 1-, 3-, 5-year DFS rates were 49%, 24.5% and 9.1% in BCLC-A patients, 7.5%, 0% and 0% in BCLC-B patients, respectively. No BCLC-C patients survived 1 year after surgery. Multivariate analysis indicated that hepatitis B surface antigen (HBsAg), vascular invasion, intra-hepatic metastasis, curative resection, tumor rupture and pathologic differentiation were independent prognostic factors. Conclusions: Surgery is effective and safe for patients with HCC >10 cm with sufficient hepatic reserve.

Variations of Available Nitrogen along the Upland Meadow in Wugong Mountain

Zhiyang Yuan,Zhi Li,Dekui Niu,Wenyuan Zhang,Xiaomin Guo,Xu Chen,Xiao Cheng 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Research on Thymopentin Loaded Oral N-Trimethyl Chitosan Nanoparticles

Xiao-jia Yuan,Zhi-rong Zhang,Qing-guo Song,Qin He 대한약학회 2006 Archives of Pharmacal Research Vol.29 No.9

Peptides, although high efficacy and specificity in their physiological function, usually have low therapeutical activities due to their poor bioavailability when administrated orally. Nanoparticles have been regarded as a useful vector for targeted drug delivery system because they can protect drug from being degraded quickly and pass the gastrointestinal barriers. Here we described a novel oral N-trimethyl chitosan nanoparticles formulation containing thymopentin (Tp5-TMC-NP). N-trimethyl chitosan (TMC) was synthesized and then used to prepare Tp5- TMC-NP by ionotropic gelation. A three-factor, five-level CCD (Central Composite Design) design was used in the optimization procedure, with HPLC as the analyzing method. The resulting Tp5-TMC-NP had a regular spherical surface and a narrow particle size range with a mean diameter of 110.6 nm. The average entrapment efficiency was 78.8%. The lyophilized Tp5-TMC-NP formulation was stable in 4oC or -20oC after storage of 3 months without obvious changes in morphology, particle size, pH and entrapment ratio. The results of the flow cytometer determination showed that the ratio of CD4+/CD8+ of Wistar female rat givenTp5-TMC-NP (ig) was 2.59 time that of the group given Tp5 (ig).

Synthesis and characterization of monodisperse yttrium aluminum garnet (YAG) micro-crystals with rhombic dodecahedron

Xu, Tao,Yuan, Rui,Xu, Peng-Cheng,Pan, Dong-Jie,Yang, Woochul,Guo, Hai-Bo,Shen, Yu-Fang,Hu, Jian-Feng,Zhang, Zhi-Jun,Zhao, Jing-Tai Elsevier 2018 Journal of alloys and compounds Vol.762 No.-

<P><B>Abstract</B></P> <P>Uniform, monodisperse yttrium aluminum garnet (YAG) rhombic dodecahedron micro-crystals were synthesized using coprecipitation and hydrothermal methods. The Na<SUB>2</SUB>SO<SUB>4</SUB> as a surfactant was helpful in improving the dispersity of the crystallites and forming well-faceted, micro-sized dodecahedral YAG crystallites. The effect of the reaction time and the solvent has also been studied. The crystal growth mechanism called dissolution/crystallization was determined by analyzing the experimental and simulated results. And the specific well-developed crystal facets in the {110} family have been demonstrated by the theoretical calculation. A higher integrated emission intensity can be achieved for the YAG:Ce crystallites by improving the phase purity, morphology with better developed dodecahedrons, and dispersion. Furthermore, a rather high luminous efficacy (LE = 104.14 lm/W) for the white LED fabricated using the YAG:Ce<SUP>3+</SUP> phosphor prepared in this work was realized. The correlation between the crystal growth, morphology and luminescence properties of YAG crystallites has been established, which exhibits a great importance for the synthesis and application of mono-dispersed YAG crystallites.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Uniform and monodisperse YAG rhombic dodecahedron micro-crystals were synthesized. </LI> <LI> The growth mechanism of YAG rhombic dodecahedron was studied through experimental and computational simulation. </LI> <LI> The correlation between the crystal growth, morphology and luminescence of YAG crystallites was established. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Mechanical behavior and chloride resistance of cementitious composites with PE and steel fiber

Liao, Qiao,Guo, Zhen-wen,Duan, Xin-zhi,Yu, Jiang-tao,Liu, Ke-ke,Dong, Fang-yuan Techno-Press 2021 Advances in concrete construction Vol.12 No.6

The mechanical behaviors and chloride resistance performance of fiber reinforced cementitious composites (FRCC) with hybrid polyethylene (PE) and steel fiber (in total 2% by volume) were investigated. Based on micro-mechanics and fracture mechanics, the reason why the tensile strain capacity of FRCC changed obviously was obtained. Besides, the effects of the total surface area of fiber in FRCC on compressive strength and chloride content were clarified. It is found that the improvement of the tensile strain capacity of FRCC with hybrid fiber is attributed to the growth of strain-hardening performance index (the ratio of complementary energy to crack tip toughness). As the total surface area of fiber related with the interfacial transition zone (ITZ) between fiber and matrix increases, compressive strength decreases obviously. Since the total surface area of fiber is small, the chloride resistance performance of FRCC with hybrid PE and steel fiber is better than that of FRCC containing only PE fiber.

Study on Soil Eco-hydrological Function during the Degradation Process of Mountain Meadow

Zhang Xue-Ling,Guo Xiao-Min,Niu De-Kui,Zhang Wen-Yuan,Li Zhi 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6